Increasing number of Salmonella paratyphi B isolates from slaughtered poultry sent in to the national Salmonella reference laboratory.]     

C Dorn  A Schroeter  A Miko  D Protz  R Helmuth 《Berliner und Münchener tier?rztliche Wochenschrift》2001,114(5-6):179-183

In the last years the number of isolations of Salmonella enterica subspecies enterica serovar paratyphi B (S. paratyphi B) sent to the national salmonella reference laboratory of Germany has increased steadily. Most of the isolates originated from fowl or poultry products. The bacteriological, serological and biochemical properties of the isolates were investigated. Special emphasis was given to the utilization of d-tartrate which subgroups the serovar. All of them belonged to the d-tartrate positive variant, which is generally considered less virulent for humans and was formerly called S. java. The performance of various tests is compared and in addition the possibility of the spread within the production line is discussed.  相似文献   

Detection and significance of tetracycline residues in eggs     

J Frieser  W Gedek  P Dorn 《DTW. Deutsche tier?rztliche Wochenschrift》1986,93(1):17-20

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Estimation of the costs of mastitis, using National Animal Health Monitoring System and milk somatic cell count data     

J K Lightner  G Y Miller  W D Hueston  C R Dorn 《Journal of the American Veterinary Medical Association》1988,192(10):1410-1413

Data collected by the National Animal Health Monitoring System in Ohio for a 12-month period during 1986 and 1987 were used to determine the relative magnitude of costs associated with mastitis in the following categories: milk production loss, veterinary services, drugs, producer labor, and "other" factors. The cost of milk loss associated with mastitis that was reported by producers cooperating in the National Animal Health Monitoring System program was compared with estimates based on bulk tank somatic cell counts and individual cow milk somatic cell counts. Using producer-reported estimates, milk loss accounted for about one third of the total cost associated with mastitis. When estimates of milk loss were replaced by estimates based on bulk tank somatic cell counts, milk loss accounted for over 80% of the total cost of mastitis. Estimates of the cost of milk loss based on studies relating milk yield to somatic cell counts differed considerably. Consequently, it was unclear how to best estimate the relative magnitude of the milk loss component of mastitis costs.  相似文献   

Stable carbon isotope ratios of rock varnish organic matter: a new paleoenvironmental indicator     

Dorn RI  Deniro MJ 《Science (New York, N.Y.)》1985,227(4693):1472-1474

Stable carbon isotope ratios of organic matter in rock varnishes of Holocene age from western North America and the Middle East show a strong association with the environment. This isotopic variability reflects the abundance of plants with different photosynthetic pathways in adjacent vegetation. Analyses of different layers of varnish on late Pleistocene desert landforms indicate that the carbon isotopic composition of varnish organic matter is a paleoenvironmental indicator.  相似文献   

Time-frequency and complexity analyses for differentiation of physiologic murmurs from heart murmurs caused by aortic stenosis in Boxers   总被引：1，自引：0，他引：1  

Höglund K  Ahlstrom CH  Häggström J  Ask PN  Hult PH  Kvart C 《American journal of veterinary research》2007,68(9):962-969

OBJECTIVE: To investigate whether time-frequency and complexity analyses of heart murmurs can be used to differentiate physiologic murmurs from murmurs caused by aortic stenosis (AS) in Boxers. ANIMALS: 27 Boxers with murmurs. PROCEDURES: Dogs were evaluated via auscultation and echocardiography. Analyses of time-frequency properties (TFPs; ie, maximal murmur frequency and duration of murmur frequency > 200 Hz) and correlation dimension (T(2)) of murmurs were performed on phonocardiographic sound data. Time-frequency property and T(2) analyses of low-intensity murmurs in 16 dogs without AS were performed at 7 weeks and 12 months of age. Additionally, TFP and T(2) analyses were performed on data obtained from 11 adult AS-affected dogs with murmurs. RESULTS: In dogs with low-intensity murmurs, TFP or T(2) values at 7 weeks and 12 months did not differ significantly. For differentiation of physiologic murmurs from murmurs caused by mild AS, duration of murmur frequency > 200 Hz was useful and the combination assessment of duration of frequency > 200 Hz and T(2) of the murmur had a sensitivity of 94% and a specificity of 82%. Maximal murmur frequency did not differentiate dogs with AS from those without AS. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that assessment of the duration of murmur frequency > 200 Hz can be used to distinguish physiologic heart murmurs from murmurs caused by mild AS in Boxers. Combination of this analysis with T(2) analysis may be a useful complementary method for diagnostic assessment of cardiovascular function in dogs.  相似文献   

Binding Sites for Ca2+-Channel Effectors and Ryanodine in Periplaneta americana—Possible Targets for New Insecticides     

Melanie Schmitt  Andreas Turberg  Michael Londershausen  August Dorn 《Pest management science》1996,48(4):375-388

The calcium channel and the ‘calcium release channel’ of muscle membrane of the cockroach Periplaneta americana have been characterized. Biological assays with calcium channel blockers and ryanodine on different insects and acari revealed pronounced insecticidal effects with ryanodine, but not with calcium channel blockers, at concentrations between 0·1 and 300 μg ml⁻¹. Skeletal muscle membranes derived either from the tubular network or from the sarcoplasmatic reticulum of P. americana were characterized with respect to the binding of the dihydropyridine (DHP) [³H]isradipine (PN 200-110), the phenyl-alkylamine [³H]verapamil and the alkaloid [³H]ryanodine. Preliminary binding studies with the benzothiazepine [³H]diltiazem suggest a low-affinity binding site with a IC₅₀ value of 3·3 μM . All binding sites tested were sensitive to treatment with proteinase K. Optimal conditions for binding of the radioligand ryanodine revealed the highest specific binding at pH 8 and at calcium chloride concentrations between 100 and 500 μM . EGTA at 10 μM abolished 95% of the ryanodine binding. Binding studies with calcium channel binding sites revealed a pronounced effect of low Ca²⁺ concentrations on specific isradipine binding, whereas verapamil and diltiazem binding were only reduced by the presence of 200 μM EGTA. With respect to high Ca²⁺ concentrations, specific binding of diltiazem, isradipine and verapamil was reduced by 73, 40 and 20%, respectively, at 5 mM Ca²⁺. Radioligand binding experiments showed high-affinity binding sites for ryanodine and isradipine. K_D values of 0·95 nM (B_max=550 fmol mg⁻¹ protein) and 0·75 nM (B_max=213 fmol mg⁻¹ protein) were determined respectively. A lower-affinity binding site was identified in binding studies with verapamil (K_D=7·4 nM and B_max=27 fmol mg⁻¹ protein). [³H]isradipine displacement studies with several dihydropyridines revealed the following ranking of affinity: nitrendipine>isradipine>Bay K8664≪nicardipine. Displacement of [³H]verapamil binding by effectors of the phenylalkylamine binding site showed that bepridil and S(-)verapamil had the highest affinities of the compounds tested followed by (±)verapamil, nor-methylverapamil and R(+)verapamil.  相似文献   

Respiration pattern and microbial use of field-grown transgenic Bt-maize residues     

Markus Raubuch  Katja Roose  Florian Wichern 《Soil biology & biochemistry》2007,39(9):2380-2389

A 49-day incubation experiment was carried out with the addition of field-grown maize stem and leaf residues to soil at three different temperatures (5, 15, and 25 °C). The aim was to study the effects of two transgenic Bt-maize varieties in comparison to their two parental non-Bt varieties on the mineralization of the residues, on their incorporation into the microbial biomass and on changes in the microbial community structure. The stem and leaf residues of Novelis-Bt contained 3.9 μg g⁻¹ dry weight of the Bt toxin Cry1Ab and those of Valmont-Bt only 0.8 μg g⁻¹. The residues of the two parental non-Bt varieties Nobilis and Prelude contained higher concentrations of ergosterol (+220%) and glucosamine (+190%) and had a larger fungal C-to-bacterial C ratio (+240%) than the two Bt varieties. After adding the Bt residues, an initial peak in respiration of an extra 700 μg CO₂-C g⁻¹ soil or 4% of the added amount was observed in comparison to the two non-Bt varieties at all three temperatures. On average of the four varieties, 19-38% of the maize C added was mineralized during the 49-day incubation at the three different temperatures. The overall mean increase in total maize-derived CO₂ evolution corresponded to a Q₁₀ value of 1.4 for both temperature steps, i.e. from 5 to 15 °C and from 15 to 25 °C. The addition of maize residues led to a strong increase in all microbial properties analyzed. The highest contents were always measured at 5 °C and the lowest at 25 °C. The variety-specific contents of microbial biomass C, biomass N, ATP and adenylates increased in the order Novelis-Bt ? Prelude<Valmont-Bt ? Nobilis. The mineralization of Novelis-Bt residues with the highest Bt concentration and lowest N concentration and their incorporation into the microbial biomass was significantly reduced compared to the parental non-Bt variety Nobilis. These negative effects increased considerably from 5 to 25 °C. The transgenic Bt variety Valmont did not show further significant effects except for the initial peak in respiration at any temperature.  相似文献   

The permeation barrier of plant cuticles: uptake of active ingredients is limited by very long‐chain aliphatic rather than cyclic wax compounds     

Simona Staiger  Pascal Seufert  Katja Arand  Markus Burghardt  Christian Popp  Markus Riederer 《Pest management science》2019,75(12):3405-3412

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Ripening reduces the shrinkage of processed dredged material     

Oing  Katja  Gröngröft  Alexander  Eschenbach  Annette 《Journal of Soils and Sediments》2020,20(1):571-583

Purpose

The utilization of dredged material in dike construction as a substitute for traditionally used materials is considered as an option to preserve natural resources such as marsh sediments. As a prerequisite for this application, the equivalency with respect to soil physical and mechanical properties of the materials must be assessed. Previous investigations have shown pronounced differences in shrinkage behavior and desiccation cracking between sediments and dredged material. The key objective of the study was to assess whether shrinkage of processed dredged material can be reduced by further processing, i.e., dewatering, which can be referred to as ripening.

Materials and methods

To compare the shrinkage behavior of the materials, three different methods of different scales were applied. Small-scale methods conducted were the standard procedure for the determination of the shrinkage limit and the determination of the coefficient of linear extensibility (COLE_rod). Large-scale shrink-swell experiments were carried out in a specially constructed test system with 90 l capacity for a period of up to 385 days. Here the materials were ripened, i.e., air-dried, until shrinkage almost ceased, and a rewetting-air-drying cycle was conducted. Shrinkage and swelling were determined during the processes by measuring the changes in volume. On the ripened materials, COLE_rod was determined.

Results and discussion

The experiments show that the shrinkage behavior of processed dredged material can be ameliorated by ripening. COLE_rod of the ripened materials were about 20–80% lower than COLE_rod of the un-ripened materials. The large-scale shrink-swell experiments showed that shrinkage in the second drying cycle amounted to less volume than in the first drying cycle and that shrinkage behavior in contrast to the first drying cycle, where pronounced proportional shrinkage was observed, was dominated by structural and residual shrinkage in this cycle.

Conclusions

Ripening of processed dredged material is considered a useful pre-treatment option to ameliorate the shrink-swell behavior of processed dredged material and to obtain a better functional equivalency with traditionally used dike construction materials such as fine-grained marsh sediments.

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Antibody response to Raboral VR-G® oral rabies vaccine in captive and free-ranging black-backed jackals (Canis mesomelas)     

Katja N. Koeppel  Peter Geertsma  Brian F. Kuhn  Ockert L. van Schalkwyk  Peter N. Thompson 《The Onderstepoort journal of veterinary research》2022,89(1)

Rabies is a zoonotic disease that remains endemic in large parts of southern Africa because of its persistence in wildlife and domestic dog vectors. The black-backed jackals (Canis mesomelas) is primarily the wildlife vector responsible for rabies outbreaks in northern parts of South Africa. Two trials were carried out to investigate antibody responses to the oral rabies vaccine Raboral V-RG® in black-backed jackals under captive and free-ranging conditions. In captive jackals 10/12 (83%; 95% confidence interval [CI]: 52% – 98%), seroconverted after single oral vaccination. Nine captive jackals had protective antibody titres (> 0.5 IU/mL) at 4 weeks (median: 2.1 IU/mL; inter quartile range [IQR]: 0.6–5.7) and 10 jackals had at 12 weeks (median: 3.5 IU/mL; IQR: 1.5–8.3) and three maintained antibody titres for up to 48 weeks (median: 3.4 IU/mL; IQR: 2.0–6.3). Four sites were baited with Raboral V-RG® vaccine for wild jackals, using fishmeal polymer and chicken heads. Baits were distributed by hand or from vehicle at three sites in north-eastern South Africa, with an average baiting density of 4.4 baits/km² and at one site in central South Africa, at 0.12 baits/km². This resulted in protective antibody titres in 3/11 jackals (27%; 95% Cl: 6–61) trapped between 3 and 12 months after baiting in north-eastern South Africa, compared with 4/7 jackals (57%; 95% Cl: 18–90) trapped after 3–18 months in central South Africa. This study shows the potential utility of oral rabies vaccination for the control of wildlife-associated rabies in north-eastern and central South Africa, but extensive studies with wider distribution of bait are needed to assess its potential impact on rabies control in wild jackals.  相似文献