Research note: Postnatal development of electrolyte transport in calf rumen as affected by weaning time.     

G Breves  R Zitnan  B Schr?der  C Winckler  H Hagemeister  K Failing  J Voigt 《Archives of Animal Nutrition》2002,56(5):371-377

In a previous study we found a positive correlation between early weaning in calves and morphological parameters which were indicative of ruminal development, i.e. the length and width of the papillae. The objective of the present study was to determine to what extent this observation could be reflected by modulations of absorptive and secretory functions of the rumen mucosa. For this purpose the short-circuit currents (Isc) as a measure of electrogenic net ion fluxes and the transepithelial conductances (G(T)) as a measure of the overall tissue permeability were measured in vitro applying the Ussing-chamber technique. Simultaneously, the unidirectional flux rates of sodium and chloride across rumen wall epithelia were determined in the absence of electrochemical gradients. Under these conditions, significant positive net flux rates (Jnet) clearly indicate active mechanisms for electrolyte absorption. For the experiments 12 male Holstein calves 7 d of age were assigned to three groups of 4 animals each: milk group (I, slaughtered after 6 weeks of age), late weaning group (II, slaughtered after 9 weeks of age) and early weaning group (II, weaned after 6 weeks of age and slaughtered after 9 weeks of age). Whereas G(T) values remained unaffected by different age and feeding, Isc values were significantly affected by early weaning but were not influenced by age. Irrespective of weaning time active absorption of Na+ tended to be higher by about 60% in 9 weeks old animals. Active absorption of chloride was significantly increased in milk fed 9 weeke old calves and this effect was further stimulated by early weaning. In conclusion, the data show an increasing active Na+ absorption with age in calf rumen that could not be influenced by early weaning. Similarly, active Cl- absorption was initially increased during postnatal development and this effect could be stimulated further by early weaning.  相似文献   

Time-frequency and complexity analyses for differentiation of physiologic murmurs from heart murmurs caused by aortic stenosis in Boxers   总被引：1，自引：0，他引：1  

Höglund K  Ahlstrom CH  Häggström J  Ask PN  Hult PH  Kvart C 《American journal of veterinary research》2007,68(9):962-969

OBJECTIVE: To investigate whether time-frequency and complexity analyses of heart murmurs can be used to differentiate physiologic murmurs from murmurs caused by aortic stenosis (AS) in Boxers. ANIMALS: 27 Boxers with murmurs. PROCEDURES: Dogs were evaluated via auscultation and echocardiography. Analyses of time-frequency properties (TFPs; ie, maximal murmur frequency and duration of murmur frequency > 200 Hz) and correlation dimension (T(2)) of murmurs were performed on phonocardiographic sound data. Time-frequency property and T(2) analyses of low-intensity murmurs in 16 dogs without AS were performed at 7 weeks and 12 months of age. Additionally, TFP and T(2) analyses were performed on data obtained from 11 adult AS-affected dogs with murmurs. RESULTS: In dogs with low-intensity murmurs, TFP or T(2) values at 7 weeks and 12 months did not differ significantly. For differentiation of physiologic murmurs from murmurs caused by mild AS, duration of murmur frequency > 200 Hz was useful and the combination assessment of duration of frequency > 200 Hz and T(2) of the murmur had a sensitivity of 94% and a specificity of 82%. Maximal murmur frequency did not differentiate dogs with AS from those without AS. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that assessment of the duration of murmur frequency > 200 Hz can be used to distinguish physiologic heart murmurs from murmurs caused by mild AS in Boxers. Combination of this analysis with T(2) analysis may be a useful complementary method for diagnostic assessment of cardiovascular function in dogs.  相似文献   

Distribution and diversity of banana (Musa spp.) in Wadi Tiwi,northern Oman     

Stephan Behrendt  Alexandra zum Felde  Edmond De Langhe  Sulaiman Al Khanjari  Katja Brinkmann  Andreas Buerkert 《Genetic Resources and Crop Evolution》2015,62(8):1135-1145

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Barrier island forest ecosystem: role of meteorologic nutrient inputs     

Art HW  Bormann FH  Voigt GK  Woodwell GM 《Science (New York, N.Y.)》1974,184(4132):60-62

The Sunken Forest, located on Fire Island, a barrier island in the Atlantic Ocean off Long Island, New York, is an ecosystem in which most of the basic cation input is in the form of salt spray. This meteorologic input is sufficient to compensate for the lack of certain nutrients in the highly weathered sandy soils. In other ecosystems these nutrients are generally supplied by weathering of soil particles. The compensatory effect of meteorologic input allows for primary production rates in the Sunken Forest similar to those of inland temperate forests.  相似文献   

Pluripotent stem cells--model of embryonic development,tool for gene targeting,and basis of cell therapy   总被引：2，自引：0，他引：2  

Prelle K  Zink N  Wolf E 《Anatomia, histologia, embryologia》2002,31(3):169-186

Embryonic stem (ES) cells are pluripotent cell lines with the capacity of self-renewal and a broad differentiation plasticity. They are derived from pre-implantation embryos and can be propagated as a homogeneous, uncommitted cell population for an almost unlimited period of time without losing their pluripotency and their stable karyotype. Murine ES cells are able to reintegrate fully into embryogenesis when returned into an early embryo, even after extensive genetic manipulation. In the resulting chimeric offspring produced by blastocyst injection or morula aggregation, ES cell descendants are represented among all cell types, including functional gametes. Therefore, mouse ES cells represent an important tool for genetic engineering, in particular via homologous recombination, to introduce gene knock-outs and other precise genomic modifications into the mouse germ line. Because of these properties ES cell technology is of high interest for other model organisms and for livestock species like cattle and pigs. However, in spite of tremendous research activities, no proven ES cells colonizing the germ line have yet been established for vertebrate species other than the mouse (Evans and Kaufman, 1981; Martin, 1981) and chicken (Pain et al., 1996). The in vitro differentiation capacity of ES cells provides unique opportunities for experimental analysis of gene regulation and function during cell commitment and differentiation in early embryogenesis. Recently, pluripotent stem cells were established from human embryos (Thomson et al., 1998) and early fetuses (Shamblott et al., 1998), opening new scenarios both for research in human developmental biology and for medical applications, i.e. cell replacement strategies. At about the same time, research activities focused on characteristics and differentiation potential of somatic stem cells, unravelling an unexpected plasticity of these cell types. Somatic stem cells are found in differentiated tissues and can renew themselves in addition to generating the specialized cell types of the tissue from which they originate. Additional to discoveries of somatic stem cells in tissues that were previously not thought to contain these kinds of cells, they also appear to be capable of developing into cell types of other tissues, but have a reduced differentiation potential as compared to embryo-derived stem cells. Therefore, somatic stem cells are referred to as multipotent rather than pluripotent. This review summarizes characteristics of pluripotent stem cells in the mouse and in selected livestock species, explains their use for genetic engineering and basic research on embryonic development, and evaluates their potential for cell therapy as compared to somatic stem cells.  相似文献   

Biomass equations for seven different tree species growing in coppice-with-standards forests in Central Germany     

Katja Albert  Peter Annighöfer  Jens Schumacher  Christian Ammer 《Scandinavian Journal of Forest Research》2014,29(3):210-221

With an increasing demand of sustainable raw materials for bioenergy use, coppicing as management approach to increase the biomass production of forests is becoming of greater importance. This study describes the parameterization of biomass equations for six tree species traditionally used in coppices forests, namely sycamore maple (Acer pseudoplatanus L.), field maple (Acer campestre L.), European ash (Fraxinus excelsior L.), European hornbeam (Carpinus betulus L.), downy birch (Betula pubescens Ehrh.), and common hazel (Corylus avellana L.) growing in coppice-with-standard systems in Lower Saxony, Germany. The parameterization was based on measurements of over 950 trees sampled from two forest sites. The sampled trees were felled and separated into three biomass compartments (stem, coarse branches, and fine brushwood) and weighed on site. The dry weight of sub samples from each compartment was measured. Equations were derived for total aboveground biomass, stem biomass, and crown biomass using regression analyses. We either used diameter at breast height as single independent explanatory variable or in combination with tree height. Biomass production of stump sprouts and generatively grown stems was compared for ash and sycamore maple. In the same age classes, it was found that ash stump sprouts had a slightly higher production than seed-grown stems. For sycamore maple, no difference was detected.  相似文献   

Eradication of ovine pulmonary adenocarcinoma by motherless rearing of lambs     

Voigt K  Krämer U  Brügmann M  Dewar P  Sharp JM  Ganter M 《The Veterinary record》2007,161(4):129-132

The principles of maedi-visna eradication programmes were applied to a field trial for the eradication of ovine pulmonary adenocarcinoma (OPA). In two maternal flocks the prevalence of gross and histological lesions in slaughtered animals was 18.3 per cent and 29.8 per cent, respectively. The lambing period was supervised for three consecutive years from 1999 to 2001, during which the lambs were taken away from their mothers at birth, deprived of maternal colostrum, and hand-reared away from other sheep. Over the three-year period, 322 hand-reared animals, mainly male lambs between 10 and 14 months old, were slaughtered; their lungs were examined grossly, 52.5 per cent of them were examined histologically, and 105 samples of caudal mediastinal lymph nodes were examined by PCR. No OPA tumours were detected in the slaughter specimens from the derived flock, but one lamb had histological lesions in one lung location; intrauterine transmission was ruled out in this case. No clinical OPA has subsequently been observed in the hand-reared flock. Bronchoalveolar lavage samples from the breeding stock were examined by PCR in order to rule out further subclinical cases of OPA. No Jaagsiekte retrovirus was detected in any of the 488 samples.  相似文献   

Potential effects of acid precipitation on soils in the humid temperate zone     

C. R. Frink  G. K. Voigt 《Water, air, and soil pollution》1977,7(3):371-388

Unmanaged soils in the northeast United States generally tend toward increasing acidity which results from several natural processes in the soil. Possible effects of acid precipitation on these processes may be caused by hydrogen ions associated with excess anions in the precipitation and, in addition, the anions may also have specific effects. This is especially true for N and S both of which have important biological implications. It is likely that direct effects of the H ion will be modest. As an example, the acidity in 114 cm of rainfall per year (Connecticut's average) with a pH of 4.3 would require 28.5 kg ha ^?1 of limestone for neutralization. By contrast agronomic practice in Connecticut frequently dictates limestone additions as high as several thousand kg ha^?1 to neutralize acidity generated by a combination of natural processes and fertilizer amendments. Studies of rainfall composition in Connecticut from 1929 to 1948 showed no clear trends with time and indicated levels of sulfate and nitrate in rainwater similar to those reported today. In a 10 yr lysimeter study in Connecticut in which tobacco was grown using conventional fertilization techniques, soil pH decreased from 5.4 to 5.0 and both gains and losses of soil cations were recorded. Thus it appears that changes in unmanaged soils induced by acid precipitation alone will be modest and subtle and probably cannot be expressed completely by measuring changes in soil pH value.  相似文献   

Toward the recognition of structure-function relationships in galactomannans     

Daas PJ  Grolle K  Van Vliet T  Schols HA  De Jongh HH 《Journal of agricultural and food chemistry》2002,50(15):4282-4289

In this paper the determination of the physical/rheological characteristics is described for a series of commercial galactomannans of which the structural details have been reported previously. Both solubility of the galactomannans and rheological properties of galactomannan solutions and galactomannan/xanthan mixtures were determined. Using a statistical analysis approach an attempt was undertaken to recognize correlations between structural and rheological data. The best correlation found was between the abundance of galactose substituents at a regular distance (type of galactomannan) and the storage modulus (G') of mixed galactomannan/xanthan gels, underscoring the hypothesis that branching hinders the formation of a network with xanthan gum. Also, the G' for the group of locust bean gums correlated with the degree of blockiness, that is, the size and occurrence of nonsubstituted regions on the mannose backbone. In addition, galactomannans displayed an apparent decrease in gelling ability with increasing average molecular weight. That G' also relates to the type of galactomannan can therefore partly be attributed to differences in average molecular weight for the various galactomannan types. However, within the series of locust bean gums tested, also an increase of G' with molecular weight was observed. This can be explained by the decreasing number of loose ends of the polymers and the concomitant increasing efficiency in network participation with increasing molecular weight.  相似文献   

Significance of vitamin E supplementation, dietary content of polyunsaturated fatty acids, and preslaughter stress on oxidative status in pig as reflected in cell integrity and antioxidative enzyme activities in porcine muscle     

Young JF  Rosenvold K  Stagsted J  Nielsen JH  Andersen HJ 《Journal of agricultural and food chemistry》2005,53(3):745-749

The present study investigates the combined effects of feed-induced increase in polyunsaturated fatty acids (PUFA) content and/or alpha-tocopherol content in pig muscles and preslaughter stress on cell integrity. Cell integrity was determined by plasma lactate dehydrogenase (LDH) activity, and antioxidative status of muscle was measured by activities of the antioxidative enzymes catalase, superoxide dismutase, and glutathione peroxidase. Preslaughter stress increased LDH activity, reflecting loss in cell membrane integrity independent of increased content of PUFA and/or alpha-tocopherol. However, feed-induced increase of PUFA decreased the LDH activity in plasma immediately after slaughter. Catalase activity in the muscle tissue increased as a consequence of the high-PUFA diet, which may indicate an increased demand caused by introduction of oxidative labile PUFA.  相似文献