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Keld Sorensen PhD Dean P. Neely VMD PhD William Read Paul M. Grappell MD 《Journal of Equine Veterinary Science》1990,10(6):417-421
An assay for the determination of the concentration of an equine fetal protein (EQFP) in pregnant mares serum was developed and used to quantitate more than 2000 serum samples from Thoroughbred mares during pregnancy. The concentration of EQFP increased throughout pregnancy. Mares with unproblematic (normal) pregnancies were used to establish the reference values at given gestational ages (GA). Mares with pregnancy failures (n=58) were found to have EQFP concentrations outside the reference values established at given GA's; 10 were high and 48 were low. Twin pregnancies (n=21), placentitis and impending abortion (n=71) were associated with elevated concentration. Twin pregnancies were detected by elevated EQFP concentration, in one case as early as 12 days after breeding. 相似文献
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Said M.S. Massomo Hanne Nielsen Robert B. Mabagala Keld Mansfeld-Giese John Hockenhull Carmen N. Mortensen 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(8):775-789
Black rot, caused by Xanthomonas campestris pv. campestris (Xcc), is a major disease constraint to cabbage production by smallholder farmers in Africa. Variability exists within the pathogen, and yet differentiation of Xcc strains from other closely-related xanthomonads attacking crucifers is often difficult. The Biolog system, fatty acid methyl ester analysis using microbial identification system (MIS), rep-PCR and pathogenicity tests were used to identify and characterise Xcc strains from Tanzania. Great diversity was observed among Xcc strains in their Biolog and rep-PCR profiles. Specific rep-PCR genomic fingerprints were linked to some geographical areas in the country. Most of the Xcc strains were clustered in two groups based on their fatty acid profiles and symptom expression in cabbage although some deviant strains were found. Each of the methods allowed a degree of identification from species, pathovar to the strain level. Biolog and MIS identified all Xcc strains at least to the genus level. Additionally, Biolog identified 47% of Xcc strains to the pathovar and 43% to strain level, whereas MIS identified 43% of the strains to pathovar level. In the absence of a database, the utility of rep-PCR for routine diagnosis of strains was limited, although the procedure was good for delineation of Xcc to the strain level. These findings indicate the existence of Xcc strains in Tanzania that are distinct from those included in Biolog and MIS databases. The limitations noticed warrant continued improvement of databases and inclusion of pathogenicity testing, using universally susceptible cultivars, as an integral part of strain identification. 相似文献
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Andersen KE Bjergegaard C Møller P Sørensen JC Sørensen H 《Journal of agricultural and food chemistry》2005,53(14):5809-5817
The contents of raffinose family oligosaccharides (RFO) and sucrose in Brassica, Lupinus, Pisum, and Hordeum species were investigated by chemometric principal component analysis (PCA). Hordeum samples contained sucrose and raffinose, and Brassica samples all contained sucrose, raffinose, and stachyose. In addition to these, the Pisum samples contained verbascose and the Lupinus samples also contained ajugose. High stachyose and low ajugose contents were found in Lupinus albus in contrast to Lupinus angustifolius, having low stachyose and high ajugose contents. Lupinus luteus had average stachyose and ajugose contents, whereas large amounts of verbascose were accumulated in these seeds. Lupinus mutabilis had high stachyose and low ajugose contents, similar to the composition in L. albus but showing higher raffinose content. The Brassica samples also showed compositional RFO variations within the species, and subgroup formations were discovered within the investigated Brassica napus varieties. PCA results indicated compositional variations between the investigated genera and within the various species of value as chemotaxonomic defined parameters and as tools in evaluations of authenticity/falsifications when RFO-containing plants are used as, for example, feed and food additives. 相似文献
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Keld Sorensen PhD Dean P. Neely VMD PhD Paul M. Grappell MD William Read 《Journal of Equine Veterinary Science》1990,10(3)
A serological enzyme linked immunosorbent assay (ELISA) for equine antibodies to Borrelia burgdorferi, the causative agent of Lyme disease, was utilized on three groups of Thoroughbred mares 14 days after breeding. Group A mares were located in Kentucky, Groups B and C mares were in Florida.Group A were broodmares from a breeding farm, where early embryonic losses were reported by the farm to be substantially higher than previous years. Of 78 mares a total of 19 (24%) had experienced early embryonic resorption. The early embryonic loss rate was 47% (8/17) in the seropositive group and 18% (11/61) in the seronegative group. The excessive loss rate in the Lyme positive group was statistically significant, with the Chi-square test yielding a p value of .014.Group B had 51 mares that did not conceive on the first breeding; Group C consisted of 51 mares that became pregnant on the first breeding, and maintained pregnancy for at least 60 days. Group C was age and farm matched to serve as controls to Group B. Group B showed a higher incidence (22%) of elevated concentrations of antibodies against B burgdorferi, than did Group C mares (16%). 相似文献
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Andersen KE Bjergegaard C Sørensen H 《Journal of agricultural and food chemistry》2003,51(25):7234-7239
A micellar electrokinetic capillary chromatography method for determination of low molecular weight carbohydrates (dp 1-2) with an unbound carbonyl group as in aldoses or other reducing carbohydrates has been developed. Reductive amination of aldoses on the carbonyl group using tryptamine introduced a chromophor system to the carbohydrates enabling their sensitive UV detection at 220 nm and identification based on the indole group using diode array detection. Twelve carbohydrates including pentoses (d-ribose, l-arabinose, and d-xylose), hexoses (d-glucose, d-mannose, and d-galactose), deoxy sugars (l-rhamnose and l-fucose), uronic acids (d-glucuronic acid and d-galacturonic acid), and disaccharides (cellobiose and melibiose) are included in the study, using d-thyminose (2-deoxy-d-ribose) as the internal standard. Detection of all 12 carbohydrates is performed within 30 min. Linearity with correlation coefficients from 0.9864 to 0.9992 was found in the concentration range of 25-2500 micromol/L for all carbohydrates; the relative standard deviation on the migration times was between 0.27 and 0.80 min, and limits of quantification and limits of determination were in the picomole range. 相似文献
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Andersen KE Bjergegaard C Møller P Sørensen JC Sørensen H 《Journal of agricultural and food chemistry》2003,51(22):6391-6397
A rapid, easy, and reproducible capillary electrophoresis method for determination of raffinose family oligosaccharides (alpha-galactosides) was developed. Sucrose, raffinose, stachyose, verbascose, and ajugose were determined with indirect UV detection at moderate alkaline pH 9.2, using pyridine-2,6-dicarboxylic acid as background electrolyte in a sodium tetraborate buffer with added cetyltrimethylammonium bromide. The separation efficiency measured by the number of theoretical plates (N) ranged from 1.4 x 10(5) to 2.3 x 10(5). The precision of the method, measured by the relative standard deviation (RSD), was less than 0.53% for the migration times and better than 3.4% for normalized areas (NA), considering all sugars except verbascose (RSD(NA) = 11.8%). Detection limits were about 110 microg/mL, corresponding to 150-320 microM. Relative response factors (RRF) were calculated on the basis of linearity studies and used for quantification of alpha-galactosides in a lupine sample (Lupinus angustifolius). 相似文献
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Heidi Blok Frandsen Sren Krogh Jensen Hanne Maribo Keld Ejdrup Markedal Flemming Schmidt Hilmer Srensen Susanne Srensen Jens Christian Srensen 《Journal of animal physiology and animal nutrition》2019,103(3):822-835
The nutritional quality of rapeseed press cakes (RPCs) in piglet feed is closely linked to its digestibility and the content of glucosinolates. This study investigates the significance of intact glucosinolate (glc) levels and degree of glc transformations on piglets performance. Four different RPCs were made from a low glc (11 µmol/g seed DM) containing B. napus L. seed variety Lioness (RPC‐LW, RPC‐LXW, RPC‐LC, RPC‐LCD). RPC made from the variety Excalibur containing the upper level of glc (24 µmol/g seed DM) of double rapeseed and produced at higher and prolonged temperature (RPC‐UXW) served as negative control, while soya bean protein concentrate served as positive control. Piglets (8 kg) were fed ad libitum diets balanced for RPC protein content, with RPC inclusion of 84–98 g/kg (day 0–14) and 151–178 g/kg (day 15–50). Glc transformation was reduced from 42% to 24% (7.3–4.2 µmol/g RPC) when the temperature input was lowered in the warm pressing of oil, while the glc loss was less pronounced (17%) when cold pressing was applied. The following feed pelleting process further reduced Glc concentration from 11% to 40% in warm‐pressed RPCs and 54 to 85% in cold‐pressed RPCs. The RPC products replaced soya bean protein without any negative effects on performance, except for piglets served cold‐pressed RPC, which had a reduction in average daily weight gain (ADG) (5%–7%, p < 0.05, Day 15–50). RPC in the feed led to increased liver weight in all piglets (p = 0.026). This may point at long‐term effects from feeding with RPC. Intestinal absorption of intact glcs was proven by their detection in urine. In conclusion, warm‐pressed RPC can be used as feed for piglet, while the presence of active myrosinase may have a negative effect on performance and cakes should either be included in lower amounts than used in the present study (18%) or include myrosinase inactivation before use. 相似文献
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丹麦农业生产中的肥料使用及其对中国的启示 总被引：1，自引：0，他引：1
现代农业中大量使用的化肥在提高作物产量的同时,也带来了一些负面影响如土壤肥力下降、土壤板结等。文章综述了丹麦先进的肥料使用和管理方面的经验。丹麦政府通过根据作物需要制定N的允许施用量和P、K的推荐施用量、对肥料的施用季节和施肥方式进行规定、根据土壤检测的结果和作物产量调整营养元素施用量等措施较好地避免了化肥污染问题。这些先进的经验为中国解决化肥污染问题提供了借鉴。笔者提出中国在农业生产过程中应从限制化肥使用量、提高农民知识水平、提倡增施有机肥、改进施肥方式等方面避免对环境的污染。 相似文献
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