Prevalence of Mycoplasma agassizii and Chelonian herpesvirus in captive tortoises (Testudo sp.) in the United Kingdom.     

Jorge F Soares  Victoria J Chalker  Kerstin Erles  Sonya Holtby  Michael Waters  Stuart McArthur 《Journal of zoo and wildlife medicine》2004,35(1):25-33

During the months of April to August in 1999 and 2002, oral swabs were collected from 146 tortoises (Testudo sp.) in private collections in the United Kingdom and tested by polymerase chain reaction (PCR) for the presence of Mycoplasma agassizii and Chelonian herpesvirus (ChHV). The presence of M. agassizii was confirmed by restriction digestion of the PCR product. A 307-bp fragment of the ChHV UL5 homologue gene was sequenced and found to show most similarity to equine herpesvirus type 1. A prevalence of 15.8 and 8.2% was found for M. agassizii and ChHV, respectively. Comparison of the carriage of both M. agassizii and ChHV in different species of tortoises correlated the presence of M. agassizii with Testudo horsfieldii and ChHV with Testudo marginata and Testudo graeca iberia. An association of ChHV with stomatitis was also found. Mixed infections with both agents were detected. The findings further demonstrate this pathogen-tortoise association and the cross transmission of these infections if different tortoise species are housed together.  相似文献   

A novel non‐azole topical treatment reduces Malassezia numbers and associated dermatitis: a short term prospective,randomized, blinded and placebo‐controlled trial in naturally infected dogs          下载免费PDF全文

Ylva Sjöström  Paul Mellor  Kerstin Bergvall 《Veterinary dermatology》2018,29(1):14-e7

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Inheritance, mode of inheritance, and candidate genes for primary hyperparathyroidism in Keeshonden     

Goldstein RE  Atwater DZ  Cazolli DM  Goldstein O  Wade CM  Lindblad-Toh K 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(1):199-203

BACKGROUND: Primary hyperparathyroidism (PHPT) is caused by inappropriate secretion of parathyroid hormone (PTH) by autonomously functioning neoplastic or hyperplastic parathyroid "chief" cells. Keeshonden are thought to be over-represented in studies on canine PHPT, but no proof of heritability or mode of inheritance has been published. The canine disease clinically resembles human familial isolated hyperparathyroidism (FIHP). HYPOTHESIS: Primary hyperparathyroidism in Keeshonden is genetically transmitted and is caused by a mutation in 1 of 4 genes implicated in human FIHP: MEN1, CASR, HRPT2, or RET. ANIMALS: Pedigrees consisting of 1647 Keeshonden were created including 219 Keeshonden with known PHPT phenotypes (69 positive). DNA samples were obtained from 176 of the 219 Keeshonden (34 positive). METHODS: Heritability and mode of inheritance were determined by segregation analysis. Canine homologs to the human genes were identified. Exons and surrounding intron regions were sequenced and scanned for sense-altering polymorphisms or polymorphisms that segregated with the disease. Messenger RNA from a parathyroid tumor of an affected Keeshond was analyzed for polymorphisms and splice alterations. RESULTS: PHPT follows an autosomal dominant mode of inheritance in Keeshonden with possible age-dependent penetrance. No polymorphisms identified in the genes analyzed were associated with a change in predicted protein or in hypothesized splice sites. CONCLUSIONS AND CLINICAL IMPORTANCE: PHPT is an autosomal dominant, genetically transmitted disease in Keeshonden. Once the mutation locus is identified, genetic testing should quickly decrease the incidence of PHPT in this breed. It is unlikely that mutations in MEN1, CASR, HRPT2, or RET cause PHPT in Keeshonden.  相似文献   

Responses of the fall armyworm (Spodoptera frugiperda) to different host plants: Implications for its management strategy     

Birhanu Sisay  Subramanian Sevgan  Christopher W. Weldon  Kerstin Krüger  Baldwyn Torto  Amanuel Tamiru 《Pest management science》2023,79(2):845-856

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Effects of pulsation rate and viscosity on pulsation-induced taste enhancement: new insights into texture-taste interactions     

Burseg KM  Camacho S  Bult JH 《Journal of agricultural and food chemistry》2011,59(10):5548-5553

Oral stimulation with high-tastant concentrations that are alternared with low-tastant concentrations or water rinses (pulsatile stimulation) results in taste intensity ratings that are higher than continuous stimulation with the same average tastant concentration. This study tested the combined effects of taste pulsation rate and viscosity on pulsation-induced taste enhancement in apple juice. According to a tastant-kinetics hypothesis, less pulsation-induced taste enhancement is expected at enhanced pulsation rates in the high-viscous proximal stimulus compared to lower viscous stimuli. High-concentration sucrose apple juice pulses and low-concentration sucrose apple juice intervals were alternated at different pulsation periods (pulse + interval in seconds) every 2.5 s (period length = 5 s) or every 1.25 s (period length = 2.5 s). Pulsed stimuli were presented at two viscosity levels by the addition of pectin (0 and 10 g/L). Sweetness intensities of pulsed stimuli were compared to a continuous reference of the same net but nonalternating sucrose concentration. Sweetness ratings were higher for pulsatile stimuli than for continuous stimuli. In low-viscous stimuli, enhancement depended on the pulsation period and peaked at 5 s periods. In high-viscous stimuli, the same enhancement was observed for both pulsation periods. These results contradict a tastant-kinetics hypothesis of viscosity-induced taste suppression because impaired tastant kinetics by viscosity would predict the opposite: lower pulsation-induced taste enhancement for viscous stimuli, especially at higher pulsation rates. Instead, these observations favor an explanation based on perceptual texture-taste interactions, which predict the observed independence between viscosity and pulsation rate.  相似文献   

Tundra plants protect the soil surface from UV     

Kevin A. Hughes  Kerstin Scherer  Petra Rettberg  Pete Convey 《Soil biology & biochemistry》2006,38(6):1488-1490

In the Arctic, seasonal ozone depletion is resulting in periods of enhanced UV-B radiation at ground level while regional climate change is associated with increasing temperatures. These changes are likely to alter plant distribution, biodiversity and morphology, which may have knock-on effects for microbially driven biogeochemical cycling and other soil processes. Our study examined the transmission of solar UV radiation through arctic tundra plants using a portable UV radiometer and the DLR-biofilm biological UV dosimeter. A strong negative correlation was found between vegetation cover and UV transmission to the soil surface. Penetration of UV to the soil beneath tundra plants varied depending upon plant morphology, being greater through low creeping plants than cushion plants, grasses or mosses. UV transmission to the soil surface beyond the foliage edge also varied with plant morphology and the presence of flowers.  相似文献   

A high-resolution radiation hybrid map of the human genome draft sequence     

Olivier M  Aggarwal A  Allen J  Almendras AA  Bajorek ES  Beasley EM  Brady SD  Bushard JM  Bustos VI  Chu A  Chung TR  De Witte A  Denys ME  Dominguez R  Fang NY  Foster BD  Freudenberg RW  Hadley D  Hamilton LR  Jeffrey TJ  Kelly L  Lazzeroni L  Levy MR  Lewis SC  Liu X  Lopez FJ  Louie B  Marquis JP  Martinez RA  Matsuura MK  Misherghi NS  Norton JA  Olshen A  Perkins SM  Perou AJ  Piercy C  Piercy M  Qin F  Reif T  Sheppard K  Shokoohi V  Smick GA  Sun WL  Stewart EA  Fernando J  Tejeda  Tran NM  Trejo T  Vo NT  Yan SC  Zierten DL  Zhao S 《Science (New York, N.Y.)》2001,291(5507):1298-1302

We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.  相似文献   

Macrolid resistance in Staphylococcus aureus strains from chicken synovitis and dermatitis in industrial production     

W Witte  H Kühn 《Archiv fuer experimentelle veterinaermedizin》1978,32(1):105-114

In outbreaks of synovitis and dermatitis in chicken caused by Staphylococcus (Staph.) aureus we observed erythromycin-resistant strains. These strains belong to the host-specific variety gallinae of Staph. aureus or can not be alloted to one of the known host-specific varieties. The macrolide-resistance-determinant is not carried by a plasmid, presumably it is located on the chromosome. The strains show constitutively cross-resistance to erythromycin, thurimycin, linomycinc and pristinamycin (M-L-S-phenotype). An ecological investigation in a chicken farm has shown that there is no distribution of Staph. aureus-strains from chicken to workers and no occurence of macrolide-resistance in Staph. aureus-strains from the workers.  相似文献   

Pentobarbital poisoning in Sumatran tigers (Panthera tigris sumatrae).     

Kerstin Jurczynski  Erhard Zittlau 《Journal of zoo and wildlife medicine》2007,38(4):583-584

Three Sumatran tigers (Panthera tigris sumatrae) at the Heidelberg Zoo in Germany presented with severe neurologic signs. Physical examination and diagnostic tests did not reveal a definitive diagnosis. Two days after initial presenting signs, all of the animals appeared clinically normal. An investigation into this outbreak revealed that all animals received horse meat on the evening before the incident. A toxicologic examination was initiated and serum analysis of the affected female tiger cub and the horse meat revealed contamination with pentobarbital.  相似文献   

Molecular characterization of the incitant of cowpea bacterial blight and pustule, Xanthomonas campestris pv. vignicola     

Valérie Verdier  Komi Assigbétsé  Gopal Khatri-Chhetri  Kerstin Wydra  Klaus Rudolph  Jean-Paul Geiger 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(6):595-602

Strains of Xanthomonas campestris pv. vignicola (Xcv), isolated from cowpea leaves with blight or minute pustules and collected from various geographic areas, were selected on the basis of pathological and physiological features. All strains were analyzed for genotypic markers by two methods: ribotyping with EcoRI endonuclease, and RFLP analysis with a plasmid probe (pthB) containing a gene required for pathogenicity from Xanthomonas campestris pv. manihotis. Ribotyping revealed a unique pattern for all the strains that corresponded to the previously described ribotype rRNA7. Based on polymorphism detected by pthB among Xcv strains, nine haplotypes were defined. The observed genetic variation was independent of the geographic origin of the strains and of pathogenic variation. Some haplotypes were widely distributed, whereas others were localized. In some cases, we could differentiate strains isolated from blight symptoms and pustules according to haplotypic composition. However, in most cases, no significant differences were observed. Our results and the previous pathogenic and biochemical characterizations suggest that the strains isolated from leaves with blight symptoms or minute pustules belong to the same pathovar. We provide information on pathogen diversity that can be used to identify and characterize resistant germplasm.  相似文献