Features of transition of perennial grasses with adaptive Triticum-type proteins into anabiosis     

A. S. Timoshchenko  V. F. Semikhov  L. O. Nurminskaya  P. N. Kharchenko 《Russian Agricultural Sciences》2014,40(1):1-4

During 1–7-day stages of ontogenesis (for example, in winter rye), biochemical changes in the aerial part of perennial grasses with adaptive Triticum-type proteins in the process of entering into the state of anabiosis were studied. It was shown that, at this point, biomass of the aerial part of plant was much higher than of the root system, and it accumulated raffinose, sucrose, glucose, and half-cystine. Synthesis of raffinose and accumulation of half-cystine continued until the stabilization of negative temperatures; as for sucrose and glucose, it ceased upon reaching the average temperature of the physiological zero, then the content of half-cystine and raffinose increased sharply, and the content of sucrose and glucose decreased at the same time. In the aerial parts of plants, fructose and phenylalanine were found, and their content increased upon reaching freezing temperatures. It was concluded that the polymerization of water-soluble proteins occurred in perennial grasses in a state of anabiosis. These proteins, along with raffinose, serve as cryoprotectants; sucrose and glucose are substrates for respiration; fructose is a substrate for respiration and protective metabolite; and phenylalanine is a substrate for the future synthesis of antioxidants.  相似文献   

Comparative analysis of anatomic structure of cotyledons for determining salt and osmotic tolerance of modified plants     

E. N. Baranova  A. A. Gulevich  V. Yu. Polyakov  P. N. Kharchenko 《Russian Agricultural Sciences》2007,33(4):236-238

The anatomic structure of cotyledons of clone 124 and cultivar Nadezhda during germination in various isosmotic sodium chloride and mannitol concentrations is compared. The possibility of using a quantitative method for revealing the character of salt tolerance in plants obtained by biotechnological methods is shown.  相似文献   

Genotyping spring rape cultivars by the microsatellite polymorphism analysis method     

Yu. V. Aniskina  A. N. Matveev  E. I. Khor℉kov  I. A. Shilov  P. N. Kharchenko 《Russian Agricultural Sciences》2007,33(5):295-297

A technology has been developed for genotyping spring rape cultivars on the basis of microsatellite analysis, which makes it possible to reliably distinguish cultivars, including those closely related, and it can be used for their passportization and certification, compiling genealogies, and protecting the intellectual property of breeders.  相似文献   

Exogenous paraoxonase‐1 during oocyte maturation improves bovine embryo development in vitro          下载免费PDF全文

JAA Rincón  EM Madeira  FT Campos  B Mion  JF Silva  VA Absalón‐Medina  WR Butler  MN Corrêa  LMC Pegoraro  A Schneider 《Reproduction in domestic animals》2016,51(5):827-830

Paraoxonase‐1 (PON1) is an enzyme found in serum and follicular fluid that protects cell membrane and circulating lipids against oxidative damage. The aims of this study were to measure the direct effects of recombinant PON1 (rPON1) on bovine oocyte maturation at the molecular level (gene expression) and to measure the carry‐over effects of PON1 on pre‐implantation embryo development in vitro. COCs were submitted to IVM with the addition of 0.0, 0.02, 0.04 and 0.08 mg ml^?1 of rPON1, corresponding to an average PON1 arylesterase enzyme activity of 2.2 ± 0.4, 15.5 ± 1.5, 30.2 ± 3.0 and 57.9 ± 5.0 U ml^?1, respectively. The results indicated that addition of rPON1 during IVM improved embryo development in a dose‐dependent manner as D7 embryo development was 22.2%, 29.4%, 32.2% and 37.0% for the treatment groups, respectively (p = 0.02). In conclusion, addition of PON1 enzyme during IVM exerted dose‐related positive effects on embryo development rates to blastocysts.  相似文献   

The influence of diel period on fish assemblage in the unstructured littoral of reservoirs     

M. ŘÍHA  J. KUBEČKA  M. PRCHALOVÁ  T. MRKVIČKA  M. ČECH  V. DRAŠTÍK  J. FROUZOVÁ  E. HOHAUSOVÁ  T. JŮZA  M. KRATOCHVÍL  J. PETERKA  M. TUŠER  M. VAŠEK 《Fisheries Management and Ecology》2011,18(4):339-347

Abstract Diel changes in littoral fish assemblage were studied in four reservoirs in the Czech Republic (Central Europe). The sampling was performed by beach seining in an unstructured littoral zone. Perch, Perca fluviatilis L., roach, Rutilus rutilus (L.), bream, Abramis brama (L.), carp, Cyprinus carpio L., ruffe, Gymnocephalus cernua (L.), pikeperch, Sander lucioperca (L.), eel, Anguilla anguilla (L.) and A. brama × R. rutilus hybrids exhibited higher densities at night. Only bleak, Alburnus alburnus (L.) exhibited higher densities during the day. The number of species was higher in night hauls, and mean body size of roach and bleak was also higher at night. Diel changes in fish densities resulted in the change in species composition between day and night. The study has implication for the sampling design to assess littoral fish assemblages using beach seine netting and recommends night sampling for a representative assessment or sampling during both diel periods for a robust assessment.  相似文献   

Reaction of triticeae cereals (spring and winter wheat and winter rye) to a change in ambient temperature in the fall-winter period of ontogeny     

A. S. Timoshchenko  V. F. Semikhov  P. N. Kharchenko  L. O. Nurminskaya 《Russian Agricultural Sciences》2011,37(6):441-446

The state of overwintering cereals during their transition into anabiosis is studied. It is shown that by the time growth processes associated with a decrease of temperature below physiological zero ceases, free amino acids are utilized, the quantitative ratio of protein amino acids changes, and raffinose, sucrose, and phenylalanine accumulate in the aboveground part of the plants. It is concluded that the transition of cereals to a state of anabiosis occurs upon maximum binding of intracellular water by the protein-carbohydrateamino acid complex after stabilization of negative ambient temperatures. The results of this work and a number of previous publications permitted formulating an idea about the structure of the physiological and biochemical mechanism of adaptation to negative temperatures acquired by perennial cereals during evolution.  相似文献   

Identification of rapeseed varieties by the microsatellite analysis method     

T. G. Satina  Yu. V. Aniskina  V. V. Karpachev  I. A. Shilov  P. N. Kharchenko 《Russian Agricultural Sciences》2010,36(1):12-14

Sixteen microsatellite loci suitable for identifying rapeseed varieties are established; 16 original primer pairs are proposed for analyzing polymorphism of these microsatellite loci. Unique genetic profiles of 13 homebred and foreign-bred rapeseed varieties are obtained.  相似文献   

Creation of a protein vector construct including an SSBTne DNA-binding domain and VirD2 nuclear localization signal     

D. V. Grishin  V. P. Gudov  O. V. Sergienko  V. G. Lunin  P. N. Kharchenko 《Russian Agricultural Sciences》2008,34(5):329-331

A genetic engineering construct is created which encodes a chimeric protein for nonviral transgenesis that includes the SSB DNA-binding domain and VirD2 nuclear localization signal. A method is developed for purifying the target protein.  相似文献   

Production of transgenic tomato plants with the Fe-dependent superoxide dismutase gene     

E. K. Serenko  V. N. Ovchinnikova  L. V. Kurenina  E. N. Baranova  A. A. Gulevich  A. N. Maisuryan  P. N. Kharchenko 《Russian Agricultural Sciences》2009,35(4):223-226

Agrobacterial transformation of tomatoes by an expression vector containing the Fe-SOD gene is carried out. The transgenic status of the transformants obtained is confirmed by PCR analysis. Differences are noted in the cell cycle and ultrastructure of transgenic and nontransgenic plants under the effect of salt stress.  相似文献   

Effect of multipurpose compost on economically valuable celery traits and soil microbiocenosis     

M. S. Gins  V. K. Gins  G. Yu. Rabinovich  N. G. Kovalev  V. A. Kharchenko 《Russian Agricultural Sciences》2009,35(5):313-315

The complex effect of multipurpose compost (MPC) on the system soil-associative microflora-plant, increasing the numbers of soil microorganisms, their enzymatic activity, and NPK content and promoting an increase of celery yield by 1.5–2 times, is revealed. In this case, the content of nitrates in organs is within the limits of the maximum allow concentration.  相似文献