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1.
A. S. Timoshchenko V. F. Semikhov L. O. Nurminskaya P. N. Kharchenko 《Russian Agricultural Sciences》2014,40(1):1-4
During 1–7-day stages of ontogenesis (for example, in winter rye), biochemical changes in the aerial part of perennial grasses with adaptive Triticum-type proteins in the process of entering into the state of anabiosis were studied. It was shown that, at this point, biomass of the aerial part of plant was much higher than of the root system, and it accumulated raffinose, sucrose, glucose, and half-cystine. Synthesis of raffinose and accumulation of half-cystine continued until the stabilization of negative temperatures; as for sucrose and glucose, it ceased upon reaching the average temperature of the physiological zero, then the content of half-cystine and raffinose increased sharply, and the content of sucrose and glucose decreased at the same time. In the aerial parts of plants, fructose and phenylalanine were found, and their content increased upon reaching freezing temperatures. It was concluded that the polymerization of water-soluble proteins occurred in perennial grasses in a state of anabiosis. These proteins, along with raffinose, serve as cryoprotectants; sucrose and glucose are substrates for respiration; fructose is a substrate for respiration and protective metabolite; and phenylalanine is a substrate for the future synthesis of antioxidants. 相似文献
2.
E. N. Baranova A. A. Gulevich V. Yu. Polyakov P. N. Kharchenko 《Russian Agricultural Sciences》2007,33(4):236-238
The anatomic structure of cotyledons of clone 124 and cultivar Nadezhda during germination in various isosmotic sodium chloride and mannitol concentrations is compared. The possibility of using a quantitative method for revealing the character of salt tolerance in plants obtained by biotechnological methods is shown. 相似文献
3.
Yu. V. Aniskina A. N. Matveev E. I. Khor℉kov I. A. Shilov P. N. Kharchenko 《Russian Agricultural Sciences》2007,33(5):295-297
A technology has been developed for genotyping spring rape cultivars on the basis of microsatellite analysis, which makes it possible to reliably distinguish cultivars, including those closely related, and it can be used for their passportization and certification, compiling genealogies, and protecting the intellectual property of breeders. 相似文献
4.
Phosphate sorption in soil is controlled largely by Fe-oxihydroxides, and so important changes in P dynamics are expected when the redox potential is modified. Such changes in P sorption when acid soil is flooded, as for rice cultivation, have been evaluated. Samples from an acid sulphate soil in the Mekong Delta of Vietnam were flooded for up to 56 d at 20°C and 30°C. Some of the samples incubated at 30°C were dried in open air for 30 d after flooding. Small redox potential (Eh<0) and pH>6 were rapidly reached in soil flooded at 30°C; less drastic reducing conditions (Eh ?0.2 V) and pH 4–5 occurred at 20°C. Phosphate sorption increased during flooding. The increase was twofold at 20°C, and 10-fold at 30°C. Phosphate sorption index decreased in the soil that was air dried after flooding at 30°C, but still remained two to three times greater than before flooding. These results were compared to the changes in oxalate-extractable Fe, i.e. poorly crystalline or amorphous Fe-oxihydroxides. The increase of P sorption per unit increase of oxalate-Fe was seven to eight fold larger at 30°C than at 20°C. 相似文献
5.
A. S. Timoshchenko V. F. Semikhov P. N. Kharchenko L. O. Nurminskaya 《Russian Agricultural Sciences》2011,37(6):441-446
The state of overwintering cereals during their transition into anabiosis is studied. It is shown that by the time growth
processes associated with a decrease of temperature below physiological zero ceases, free amino acids are utilized, the quantitative
ratio of protein amino acids changes, and raffinose, sucrose, and phenylalanine accumulate in the aboveground part of the
plants. It is concluded that the transition of cereals to a state of anabiosis occurs upon maximum binding of intracellular
water by the protein-carbohydrateamino acid complex after stabilization of negative ambient temperatures. The results of this
work and a number of previous publications permitted formulating an idea about the structure of the physiological and biochemical
mechanism of adaptation to negative temperatures acquired by perennial cereals during evolution. 相似文献
6.
T. G. Satina Yu. V. Aniskina V. V. Karpachev I. A. Shilov P. N. Kharchenko 《Russian Agricultural Sciences》2010,36(1):12-14
Sixteen microsatellite loci suitable for identifying rapeseed varieties are established; 16 original primer pairs are proposed
for analyzing polymorphism of these microsatellite loci. Unique genetic profiles of 13 homebred and foreign-bred rapeseed
varieties are obtained. 相似文献
7.
D. V. Grishin V. P. Gudov O. V. Sergienko V. G. Lunin P. N. Kharchenko 《Russian Agricultural Sciences》2008,34(5):329-331
A genetic engineering construct is created which encodes a chimeric protein for nonviral transgenesis that includes the SSB DNA-binding domain and VirD2 nuclear localization signal. A method is developed for purifying the target protein. 相似文献
8.
E. K. Serenko V. N. Ovchinnikova L. V. Kurenina E. N. Baranova A. A. Gulevich A. N. Maisuryan P. N. Kharchenko 《Russian Agricultural Sciences》2009,35(4):223-226
Agrobacterial transformation of tomatoes by an expression vector containing the Fe-SOD gene is carried out. The transgenic
status of the transformants obtained is confirmed by PCR analysis. Differences are noted in the cell cycle and ultrastructure
of transgenic and nontransgenic plants under the effect of salt stress. 相似文献
9.
M. S. Gins V. K. Gins G. Yu. Rabinovich N. G. Kovalev V. A. Kharchenko 《Russian Agricultural Sciences》2009,35(5):313-315
The complex effect of multipurpose compost (MPC) on the system soil-associative microflora-plant, increasing the numbers of
soil microorganisms, their enzymatic activity, and NPK content and promoting an increase of celery yield by 1.5–2 times, is
revealed. In this case, the content of nitrates in organs is within the limits of the maximum allow concentration. 相似文献
10.