Application of acoustic Doppler current profiler combined with a scientific echo sounder for krill Euphausia pacifica density estimation     

Kyounghoon  LEE  Tohru  MUKAI  Donhyug  KANG  Kohji  IIDA 《Fisheries Science》2004,70(6):1051-1060

ABSTRACT:   An application of the acoustic Doppler current profiler (ADCP, 153.6 kHz) in combination with a scientific echo sounder (EK60, 38 and 200 kHz) was investigated to estimate the density of krill Euphausia pacifica . The acoustic backscattering strength from sound scattering layers was compared with biomass estimates from midwater trawls. Euphausia pacifica was targeted among mixed species populations in the sound scattering layer in the offshore Funka Bay area of Hokkaido, Japan. The frequency characteristics of acoustic backscattering by krill were calculated using a distorted wave Born approximation scattering model at three frequencies. Krill aggregations identified from the EK60 data were extracted as the mean volume backscattering strength difference between two frequencies. They were then used to identify similar aggregations in the ADCP data by matching observation times and depths for the two methods, which were applied simultaneously. Results from the comparison of the mean volume backscattering strength and the density calculated from the ADCP and EK60 showed that ADCP can be used to measure density and spatial–temporal distribution of krill aggregations. Current speed and direction at the study site were found to be 16.1 cm/s and 187.0°, respectively, and krill speed and direction (including the current component) were found to be 19.8 cm/s and 172.2°, respectively. Based on the ADCP data, the net speed and direction of the krill aggregations were found to be 5.9 cm/s and 128.0°, respectively.  相似文献   

The palliative efficacy of modified Mohs paste for controlling canine and feline malignant skin wounds     

Yasuhiro Fukuyama  Tetsushi Tezuka  Atsushi Kawabata  Takuya Maruo 《The Veterinary quarterly》2016,36(3):176-182

In veterinary medicine, the management of malignant skin wounds is highly challenging. We conducted a study on seven case animals (four dogs and three cats) which presented with malignant skin wounds. All seven animals had signs and symptoms which were controlled following treatment with a modified Mohs paste. Upon obtaining informed consent from their owners, the animals requiring management of malignant wounds were enrolled in this study. The modified Mohs paste was prepared by mixing zinc chloride, zinc oxide starch powder, glycerin, and distilled water. The modified Mohs paste was topically applied to and left to remain on the malignant wounds for one hour, under controlled conditions. Once the paste was removed, the wounds were irrigated with a solution of sterile saline. At the first examination, the wounds of each animal were observed for signs of exudate, malodor, and bleeding. In every case, visible improvement was observed immediately after the modified Mohs paste treatment. Specifically, the size of the malignant wounds, and the number of times the dressing gauze required changing, significantly decreased (p < 0.05 and p < 0.01, respectively). The open malignant skin wounds caused by mammary gland tumors disappeared in two cases. The Mohs paste has been shown to be a viable option for the palliative treatment in canine and feline malignant skin wound management.  相似文献   

Abnormal structural luteolysis in ovaries of the senescence accelerated mouse (SAM): expression of Fas ligand/Fas-mediated apoptosis signaling molecules in luteal cells     

Kiso M  Manabe N  Komatsu K  Shimabe M  Miyamoto H 《The Journal of reproduction and development》2003,49(6):457-463

  相似文献   

Verification of mean volume backscattering strength obtained from acoustic Doppler current profiler by using sound scattering layer     

Kyounghoon Lee  Tohru Mukai  Daejae Lee  Kohji Iida 《Fisheries Science》2008,74(2):221-229

ABSTRACT:   Acoustic Doppler current profilers (ADCPs) have recently been used to estimate the dynamic characteristics and biomass of sound scattering layers (SSLs) or swimming speed of fish schools and to analyze SSL spatial distribution or various behavior patterns. This paper shows that it is necessary to verify mean volume backscattering strength (MVBS, dB) values acquired from each beam for quantitative analysis of the spatial distribution or the biomass estimates of such specific targets as SSL or a fish school when using an ADCP. In this study, the SSL was selected to be a homogeneous density layer over a large area and two methods were used to verify the MVBS values from each beam of the ADCP. First, a mutual comparison among four beams was conducted after calculating MVBS from the measured echo intensity. Second, the MVBS values were verified using comparison between the calculated MVBS from the 153.6 kHz ADCP and MVBS from three frequencies of a well-calibrated scientific echosounder. Moreover, the dominant scatterers (euphausiids) were collected by a framed midwater trawl. From these samples, biological data were used to identify the different frequency characteristics between two systems, using a distorted-wave Born approximation (DWBA) theoretical backscattering model in order to assess the averaged target strength and target strength TS differences for the three frequencies.  相似文献   

Anti-allergic effects of the brown alga <Emphasis Type="Italic">Eisenia arborea</Emphasis> on Brown Norway rats     

Yoshimasa Sugiura  Kohji Matsuda  Takashi Okamoto  Makoto Kakinuma  Hideomi Amano 《Fisheries Science》2008,74(1):180-186

To investigate the anti-allergic effects of the brown alga Eisenia arborea. A strain of Brown Norway rats know to strongly respond to immunoglobulin E (IgE) were used as an allergy model animal. The rats were immunized with ovalbumin by oral administration. The levels of serum IgE and histamine were suppressed in the rats fed a diet supplemented with dried E. arborea powder. As for the cytokine pattern, the interferon-γ production in the spleens and mesenteric lymph nodes (MLN) was enhanced, and the interleukin-4 (IL-4) production in the spleens and/or IL-10 production in the spleens and MLN were suppressed. These results, together with the change in the Th1/Th2 balance, indicate that the rats fed with E. arborea became more anti-allergic, suggesting that E. arborea might possess anti-allergic effects.  相似文献   

Analysis of genomic mutation and immunohistochemistry of platelet‐derived growth factor receptors in canine vascular tumours          下载免费PDF全文

S. Abou Asa  T. Mori  K. Maruo  A. Khater  A. El‐sawak  E. Abd el‐Aziz  T. Yanai  H. Sakai 《Veterinary and comparative oncology》2015,13(3):237-245

We examined whether mutation of the platelet‐derived growth factor receptor protein tyrosine kinase (PDGFR)‐α and PDGFR‐β genes contributes to their overexpression in canine vascular tumours. Genomic sequences of trans‐ or juxtamembrane regions of PDGFR‐α and PDGFR‐β were analysed with immunohistochemical staining and polymerase chain reaction‐direct sequencing using DNA from paraffin‐embedded neoplastic tissues of 27 hemangiosarcomas (HSAs) and 20 hemangiomas (HAs). Immunohistochemically, 75% of the HA cases were positive for PDGFR‐α and almost most of the HA cases were negative for PDGFR‐β. Of the HSA cases, 55.6% were negative for PDGFR‐α and 63% were strongly positive for PDGFR‐β. Among the HA cases, 1 missense mutation was detected in PDGFR‐α exon 18 and 1 in PDGFR‐β exon 17. Two HSA cases had missense mutations in exon 14 and 1 in exon 17 of PDGFR‐β. Thus, genomic mutation of trans‐ or juxtamembrane regions of PDGFRs was not the main mechanism driving the activation of receptors in HSA and HA.  相似文献   

Collection,mapping, and annotation of over 28,000 cDNA clones from japonica rice   总被引：2，自引：0，他引：2  

Rice Full-Length cDNA Consortium;National Institute of Agrobiological Sciences Rice Full-Length cDNA Project Team  Kikuchi S  Satoh K  Nagata T  Kawagashira N  Doi K  Kishimoto N  Yazaki J  Ishikawa M  Yamada H  Ooka H  Hotta I  Kojima K  Namiki T  Ohneda E  Yahagi W  Suzuki K  Li CJ  Ohtsuki K  Shishiki T;Foundation of Advancement of International Science Genome Sequencing & Analysis Group  Otomo Y  Murakami K  Iida Y  Sugano S  Fujimura T  Suzuki Y  Tsunoda Y  Kurosaki T  Kodama T  Masuda H  Kobayashi M  Xie Q  Lu M 《Science (New York, N.Y.)》2003,301(5631):376-379

  相似文献   

Analysis of KIT expression and KIT exon 11 mutations in canine oral malignant melanomas     

Murakami A  Mori T  Sakai H  Murakami M  Yanai T  Hoshino Y  Maruo K 《Veterinary and comparative oncology》2011,9(3):219-224

KIT, a transmembrane receptor tyrosine kinase, is one of the specific targets for anti-cancer therapy. In humans, its expression and mutations have been identified in malignant melanomas and therapies using molecular-targeted agents have been promising in these tumours. As human malignant melanoma, canine malignant melanoma is a fatal disease with metastases and the poor response has been observed with all standard protocols. In our study, KIT expression and exon 11 mutations in dogs with histologically confirmed malignant oral melanomas were evaluated. Although 20 of 39 cases were positive for KIT protein, there was no significant difference between KIT expression and overall survival. Moreover, polymerase chain reaction amplification and sequencing of KIT exon 11 in 17 samples did not detect any mutations and proved disappointing. For several reasons, however, KIT expression and mutations of various exons including exon 11 should be investigated in more cases.  相似文献   

Cytologically atypical anal sac adenocarcinoma in a dog     

Sakai H  Murakami M  Mishima H  Hoshino Y  Mori T  Maruo K  Yanai T 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2012,41(2):291-294

A 10-year-old intact female Shetland Sheepdog with tenesmus had a subcutaneous mass at the left ventral aspect of the anus. On cytologic examination, 2 types of cells were observed. Most of the cells were oval to polygonal and had elliptical or elongate nuclei and a moderate amount of pale to basophilic cytoplasm. The remaining cells had round to oval nuclei and pale to basophilic cytoplasm. Cells of both types were loosely adhered to each other and were arranged in rosette-like structures. Both neoplastic cell types had fine homogenous chromatin and either a small indistinct nucleolus or no visible nucleolus. Mild anisokaryosis and anisocytosis were observed. Histologically, the mass consists of glandular structures formed by cuboidal cells admixed with bundles of spindle cells. Eosinophilic PAS- and Alcian blue-positive secretory material was found in the center of some glandular structures. Both neoplastic cell types had positive staining with paradoxical concanavalin A and expressed cytokeratin, but not vimentin, S-100, α-smooth muscle actin, or desmin. Based on location and histologic and immunohistochemical features, the final diagnosis was adenocarcinoma of the apocrine gland of the anal sac, which should be included as a cytologic differential diagnosis when spindle cells and typical epithelial cells are observed in masses in the region of the anal sac of dogs.  相似文献   

Investigation of the active site of an unclassified glutathione transferase in Bombyx mori by alanine scanning     

Kohji Yamamoto  Misuzu Yamaguchi  Naotaka Yamada 《Journal of Pesticide Science》2020,45(4):238

  相似文献