Influence of Eimeria tenella infection on the cecal pH, oxidation-reduction potential and concentration of volatile fatty acids in gnotobiotic chicken     

T Fukata  T Myokai  E Baba  A Arakawa 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1989,51(2):425-427

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Search for regulatory factors of the pituitary-specific transcription factor PROP1 gene     

Naoto NISHIMURA  Hiroki UEHARU  Hiroto NISHIHARA  Shiori SHIBUYA  Saishu YOSHIDA  Masashi HIGUCHI  Naoko KANNO  Kotaro HORIGUCHI  Takako KATO  Yukio KATO 《The Journal of reproduction and development》2016,62(1):93-102

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Phylogenetic analysis of Myanmar indigenous chickens using mitochondrial D-loop sequence reveals their characteristics as a genetic resource     

Su Lai Yee Mon  Moe Lwin  Aye Aye Maw  Lat Lat Htun  Saw Bawm  Kotaro Kawabe  Yasuhiko Wada  Shin Okamoto  Takeshi Shimogiri 《Animal Science Journal》2021,92(1):e13647

Myanmar indigenous chickens play important roles in food, entertainment, and farm business for the people of Myanmar. In this study, complete mitochondrial D-loop sequences (1232 bp) were analyzed using 176 chickens, including three indigenous breeds, two fighting cock populations, and three indigenous populations to elucidate genetic diversity and accomplish a phylogenetic analysis of Myanmar indigenous chickens. The average haplotype and nucleotide diversities were 0.948 ± 0.009 and 0.00814 ± 0.00024, respectively, exhibiting high genetic diversity of Myanmar indigenous chickens. Sixty-four haplotypes were classified as seven haplogroups, with the majority being haplogroup F. The breeds and populations except Inbinwa had multiple maternal haplogroups, suggesting that they experienced no recent purifying selection and bottleneck events. All breeds and populations examined shared haplogroup F. When 232 sequences belonging to haplogroup F (79 from Myanmar and 153 deposited sequences from other Asian countries/region) were analyzed together, the highest genetic diversity was observed in Myanmar indigenous chickens. Furthermore, Myanmar indigenous chickens and red junglefowls were observed in the center of the star-like median-joining network of 37 F-haplotypes, suggesting that Myanmar is one of the origins of haplogroup F. These findings revealed the unique genetic characteristic of Myanmar indigenous chickens as important genetic resources.  相似文献   

Molecular dynamics of heterochromatin protein 1beta, HP1beta, during mouse preimplantation development     

Yamazaki T  Kobayakawa S  Yamagata K  Abe K  Baba T 《The Journal of reproduction and development》2007,53(5):1035-1041

To elucidate the molecular dynamics of HP1beta in mouse preimplantation embryos, we examined the localization, dynamics, and mobility of HP1beta in the (pro)nucleus by live cell imaging. Time-lapse observation revealed that the chromatin association of HP1beta is regulated in a cell cycle-dependent manner. HP1beta was localized in the interphase nucleus and was dynamically dissociated from the nucleus during the metaphase stage. The HP1beta assembly and clustered heterochromatin structure were both found in the nuclei of 2-cell and later-stage embryos. Moreover, fluorescent recovery after photobleaching analysis implied that HP1beta is more freely mobile in the pronucleus of the 1-cell embryo than in the 4-cell nucleus. These results suggest that the chromatin configuration may be regulated by the stability and mobility of chromatin-associated proteins including HP1beta during early embryonic stages.  相似文献   

The effect of estrogen on phosphorylation of prolactin in the mouse pituitary gland     

Horiguchi K  Naito J  Ishida M  Harigaya T 《The Journal of reproduction and development》2007,53(3):515-523

Several studies have indicated that prolactin (PRL) assumes oligomeric, proteolytically cleaved, phosphorylated and glycosylated forms. Phosphorylated PRL (PPRL) is considered to be the most important posttranslationally modified form in the rat. In the present study, we examined whether or not PRL is present in the mouse pituitary gland in the phosphorylated form. Mouse pituitary PRL was digested with acid phosphatase, resolved by two-dimensional gel electrophoresis, stained with Coomassie brilliant blue, and then immunoblotted against the anti-PRL, anti-phosphoserine and anti-phosphothreonine antibodies. We also examined whether PRL is phosphorylated by protein kinases and semi-quantified the ratios of PPRL to PRL in the pituitary gland. The results indicated that three types of PRL are present in the pituitary glands of both male and female mice. One was non-phosphorylated (isoform 1), and the other two were immunoreactive to anti-phosphoserine (isoform 2) and/or anti-phosphothreonine (isoform 3) antibodies. The ratio between isoforms 2 and 1 of the 30-day-old female mice was higher than that of the 20-day-old female mice. However, the ratios among the three isoforms in the male pituitary glands did not differ with age. The ratio of PPRL to isoform 1 was obviously reduced after ovariectomy (OVX), and it recovered with estrogen replacement. These results suggest that estrogen influences PRL phosphorylation in female mice.  相似文献   

Effect of Oyster Mushroom,Pleurotus ostreatus,Extract on Hemato‐Immunological Parameters of Rainbow Trout,Oncorhynchus mykiss          下载免费PDF全文

Gülşen Uluköy  Esİn Baba  Canan Öntaş 《Journal of the World Aquaculture Society》2016,47(5):676-684

The current study investigated the effects of oyster mushroom Pleurotus ostreatus, extract on immunological and hematological parameters of rainbow trout, Oncorhynchus mykiss. The fish were fed diets containing 0 (control), 1, and 2% supplementation of the oyster mushroom extract for 6 wk. Blood samples were collected weekly for the first 6 weeks. After 6 wk of feeding, the fish were challenged with Lactococcus garvieae and mortality was recorded. The results of this study showed that feeding rainbow trout an oyster mushroom extract–supplemented diet stimulated phagocytic activity of phagocytic cells, lysozyme activity, and myeloperoxidase activity in serum, but it did not significantly affect the total serum immunoglobulin level. At the same time, a significant increase was found in the number of neutrophils, monocytes, and total white blood cells. Fish fed the diet supplemented with oyster mushroom extract showed reduced mortalities following L. garvieae infection compared with controls. These results show that supplementation of fish diets with oyster mushroom extract at 1 and 2% concentrations significantly imrpoves hematological parameters and modulates the immune response against L. garvieae in rainbow trout.  相似文献   

Long-term case study of myelodysplastic syndrome in a dog     

Miyamoto T  Horie T  Shimada T  Kuwamura M  Baba E 《Journal of the American Animal Hospital Association》1999,35(6):475-481

A 10-year-old, female shih tzu was diagnosed as having myelodysplastic syndrome (MDS) based on the presence of a nonregenerative anemia, dysplastic changes in the three hematopoietic cell lines, a normal to hypercellular bone marrow, and less than 30% blast cells of all nucleated cells in the bone marrow. Low-dose aclarubicin, a differentiation-induction therapy for MDS and atypical leukemias in humans, was administered. Hematological improvement was observed, and the dog lived for 809 days after the first presentation.  相似文献   

Secretion of Salmonella-specific antibodies in the oviducts of hens experimentally infected with Salmonella enteritidis     

Withanage GS  Sasai K  Fukata T  Miyamoto T  Baba E 《Veterinary immunology and immunopathology》1999,67(2):185-193

The production and secretion of Salmonella enteritidis whole cell antigen-specific antibodies in the oviducts and in the serum of laying hens experimentally infected with Salmonella enteritidis, was analyzed by ELISA. The dynamics of the antibody levels in the oviducts were identical to that in the serum. Subclasses of antibodies (IgA, IgG, and IgM) in the infected hens were found to increase significantly (p < 0.01) compared to those in the control uninfected hens throughout the experiment. IgG and IgM levels in both oviducts and in sera reached to a peak by 14 days post-inoculation, and remained elevated throughout. The secretion of IgA seemed to be transient since the IgA levels increased to a peak 7 days after both primary and secondary inoculations, and declined rapidly. The elevated levels of antibodies were followed by partial clearance of Salmonella organisms from the oviducts. The present results indicate a significant local immune reaction against the Salmonella infection and suggest an association of the local antibodies with the clearance of Salmonella from the oviducts at least partially.  相似文献   

Effects of chicken thymic stromal cells on the growth and differentiation of thymocytes in vitro   总被引：2，自引：0，他引：2  

Mukamoto M  Okada T  Kodama H  Baba T 《Veterinary immunology and immunopathology》1999,68(1):25-37

We examined contact-mediated effects of chicken thymic stromal cells (TSC) on thymocyte differentiation by co-cultivation of these cell populations. The primary cultures of TSC isolated from thymus mainly have consisted of epithelial cells which were polygonal in shape, possessed long processes and expressed MHC class II antigen. When thymocytes were co-cultured with TSC, 60% to 70% of thymocytes attached to TSC and some of them engulfed underneath TSC. These attached thymocytes were CD4-CD8- and CD4+CD8+ subsets and expressed alpha/beta TCRhigh or gamma/delta TCRlow. Some of the thymocytes attaching to TSC showed an increase of intracellular and nuclear density, fragmentation of cytoplasm and nuclei, and DNA fragmentation. And also, thymocytes attaching to TSC contained a higher percentage of cycling (S and G2 + M phase) cells than nonattaching cells. These results indicate that specific subsets in thymocytes selectively bind to TSC and undergo apoptotic death or proliferation because of interaction with TSC. Chicken TSC may play an important role in thymic differentiation by direct contact within the thymus as in mammals.  相似文献   

Effects of cage contamination with coccidia and salmonella on acute salmonellosis in young chickens     

Y Kosugi  E Baba  T Fukata  A Arakawa 《Avian diseases》1986,30(2):313-318

The effects of concurrent cage contamination with Salmonella typhimurium and Eimeria tenella on the establishment of salmonella infection in day-old chickens were investigated. Chickens were divided into five groups: uninfected recipient birds placed in a cage contaminated by donor birds infected with E. tenella and S. typhimurium; E. tenella-infected recipients placed in a cage contaminated by S. typhimurium-infected donors; uninfected recipients placed in a cage contaminated by S. typhimurium-infected donors; E. tenella-infected recipients placed in a cage contaminated by uninfected donors; and uninfected recipients placed in a cage contaminated by uninfected donors. Three identical trials were conducted. Recipient birds were necropsied 4, 7, and 11 days after caging. In the cage where donor birds infected with both organisms had been reared, S. typhimurium counts in feces and number of feces positive for S. typhimurium were significantly (P less than 0.05) greater than those in the other cages on days 0, 4, and 7 after caging. Moreover, in this cage, more chicks died, counts of S. typhimurium in cecal contents were greater, and more birds were positive for S. typhimurium than in the other groups. This suggests that S. typhimurium infection in day-old chickens is enhanced in cages contaminated with E. tenella and S. typhimurium compared with infection in cages contaminated with S. typhimurium alone.  相似文献