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Nitrogen fertilizer practices affect nitrous oxide (N2O) emissions from agricultural soils. The “4R” nutrient stewardship framework of using N fertilizer at the right rate, right source, right placement and right time can reduce N2O emissions while maintaining or improving yield of field crops, but understanding of how the various factors affect N2O emissions from irrigated processing potato is lacking. We examined the effects of selected 4R practices on emissions, using results from two irrigated processing potato studies each conducted in 2011 and 2012 in Manitoba, Canada. Experiment 1 examined combinations of source (urea, ESN), placement (pre-plant incorporation [PPI], banding), and rate (100 and 200 kg N ha-1) on a clay loam soil. Experiment 2 examined timing and source treatment combinations (urea PPI, ESN PPI, urea split, urea split/fertigation) on a loamy fine sandy soil. For Experiment 1, use of ESN at 200 kg ha-1 did not reduce area-, yield- and applied fertilizer N- based N2O emissions compared to urea at 200 kg ha-1, irrespective of placement. Emissions from pre-plant banding ESN at 200 kg ha?1, however, were 32% lower than from PPI ESN. For Experiment 2, compared to single pre-plant urea application, fertigation simulated by in-season application of urea ammonium nitrate (UAN) gave lower area-, yield- and applied fertilizer N- based emissions. Split urea ( \( \raisebox{1ex}{$2$}\!\left/ \!\raisebox{-1ex}{$3$}\right. \) pre-plant, \( \raisebox{1ex}{$1$}\!\left/ \!\raisebox{-1ex}{$3$}\right. \) hilling) also reduced area- and yield- based N2O emissions compared to single pre-plant urea application. Emissions were generally lower at the site with loamy fine sandy soil than the site with clay loam soil. These results demonstrate that combinations of “4R” practices rather than source alone are best to achieve reductions in N2O emissions from irrigated potato production.  相似文献   
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Twenty-one male Broad Breasted White turkeys which had been selected for high or low blood pressure were put on an exercise regimen to determine its effect on semen quality. Eleven were from the hypertensive line and 10 from the hypotensive line. Eleven (6 hypertensive and 5 hypotensive) turkeys were placed on an exercise regime of walking (herding) about 1 mile daily for 6 weeks before the experiment began, and then for a further 6 weeks during semen collection. Semen was collected from 11 turkeys on the exercise regime and 10 nonexercised control birds which were kept in their experimental pens during the trial. Both hypertensive and exercised groups showed an improved semen colour score at the first collection period (P less than or equal to 0.09). Only the exercised group showed a significantly (P less than or equal to 0.05) better score than the control group during periods 2 and 3. The hypertensive line showed significantly higher spermatozoa count than the hypotensive line during the first collection period. There was no effect of either exercise or selection for high or low blood pressure on semen volume.  相似文献   
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A single dose of digoxin was injected, IV, into 5 mature male turkeys (0.066 mg/kg of body weight), 8 male ducks (0.066 mg/kg), and 6 roosters (0.33 mg/kg). Twenty-three serial venous blood samples were collected before (baseline) and after the administration of digoxin to turkeys, ducks, and roosters. Plasma concentrations of digoxin were determined in duplicate by a radioimmunoassay that was validated for avian species. The plasma concentrations were best fitted by a 3 (turkeys, ducks)- and 2 (roosters)-compartment open model, with first-order elimination from the central compartment. Significant (P less than 0.05) kinetic differences were determined among species. Mean half-life (t1/2) for ducks, roosters, and turkeys were 8.30 +/- 2.70 (mean +/- SD), 6.67 +/- 3.50, and 23.7 +/- 4.8 hours, respectively. The volume of distribution at steady state (Vss) was 14.7 +/- 2.9, 3.13 +/- 0.49, and 2.27 +/- 0.36 L/kg, and total body clearance (CL) of drug was 1.54 +/- 0.43, 0.461 +/- 0.187, and 0.136 +/- 0.022 L/h/kg for ducks, roosters, and turkeys, respectively. The mean residence time was 10.3 +/- 3.9, 8.37 +/- 4.97, and 16.8 +/- 2.2 hours, respectively. Volume of distribution at steady state and CL in ducks were several fold higher than that in turkeys. The terminal half-life of digoxin determined for ducks and roosters in this study was considerably shorter than those previously reported for several mammalian species.  相似文献   
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Mistakenly classifying morphologically cryptic endemic species as populations of widespread species potentially interferes with the conservation of biodiversity because undetected endemics that are imperilled may lack appropriate protection. It also impedes the reconstruction of the evolutionary history of a taxon by obscuring the number and distributional limits of species. Here, we present genetic and phylogenetic evidence corroborated by morphology that Philippine populations of seven widespread, non-migratory passerine birds might represent unrecognized, distinct species. An extrapolation based on this finding suggests that the proportion of endemic bird species in the Philippines could be much higher than currently estimated. This high degree of cryptic diversity in a well-studied, volant taxon implies that large numbers of unrecognized species can be expected in less thoroughly studied groups. We predict that genetic investigations of insular populations of widespread species will frequently reveal unrecognized island endemics, and because of the vulnerability of island habitats and their biota, these taxa may be particularly susceptible to extinction.  相似文献   
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By virtue of their accumulated genetic alterations, tumor cells may acquire vulnerabilities that create opportunities for therapeutic intervention. We have devised a massively parallel strategy for screening short hairpin RNA (shRNA) collections for stable loss-of-function phenotypes. We assayed from 6000 to 20,000 shRNAs simultaneously to identify genes important for the proliferation and survival of five cell lines derived from human mammary tissue. Lethal shRNAs common to these cell lines targeted many known cell-cycle regulatory networks. Cell line-specific sensitivities to suppression of protein complexes and biological pathways also emerged, and these could be validated by RNA interference (RNAi) and pharmacologically. These studies establish a practical platform for genome-scale screening of complex phenotypes in mammalian cells and demonstrate that RNAi can be used to expose genotype-specific sensitivities.  相似文献   
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Fundamental to our understanding of erosional and transport phenomena in earth-surface dynamics and engineering is knowledge of the conditions under which sediment motion will begin when subjected to turbulent flow. The onset criterion currently in use emphasizes the time-averaged boundary shear stress and therefore is incapable of accounting for the fluctuating forces encountered in turbulent flows. We have validated through laboratory experiments and analytical formulation of the problem a criterion based upon the impulse imparted to a sediment grain. We demonstrate that in addition to the magnitude of the instantaneous turbulent forces applied on a sediment grain, the duration of these turbulent forces is also important in determining the sediment grain's threshold of motion, and that their product, or impulse, is better suited for specifying such conditions.  相似文献   
9.
Common sites for blood sampling in the pekin duck and other avian species include the basilic vein, jugular vein, superficial plantar metatarsal vein, heart, and occipital sinus. The use of each of these sites is described and/or illustrated. In the present study using the pekin duck, the superficial plantar metatarsal vein proved most satisfactory for collecting repeated samples (every 5 minutes for 30 minutes) with minimal trauma to the duck.  相似文献   
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