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Prevalence and intensity of gastrointestinal nematodes in slaughter lambs from central Alberta 
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下载免费PDF全文 Colwell DD Goater CP Jacobson KM 《The Canadian veterinary journal. La revue veterinaire canadienne》2002,43(10):775-777
Two trichostronglyes, Teladorsagia ostertagi and Nematodiru helvetianus, accounted for > 99% of nematodes recovered from gastrointestinal tracts of 47 lambs pastured in central Alberta during the summer of 2000. Their prevalence and mean intensity increased from < 10% and < 50 worms/host, in late June, to > 80% and approximately 1000 worms/host, by mid-July, respectively. 相似文献
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Jankowski MK Ogilvie GK Lana SE Fettman MJ Hansen RA Powers BE Mitchener KM Lovett SD Richardson KL Parsley L Walton JA 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(1):105-108
Matrix metalloproteinases (MMPs) are enzymes that play key roles in angiogenesis, tumor invasion, and metastasis in a wide variety of species. The purpose of this study was to evaluate pro and active MMP 2 and 9 concentrations in tumor, normal stromal tissue, and serum from tumor-bearing cats. We hypothesized that serum concentrations of pro and active forms of MMPs 2 and 9 would be predictive of MMP concentrations in tumor tissue and that these MMP concentrations would correlate with the histopathologic grade of the malignancies. Pro and active forms of MMPs 2 and 9 were determined by gelatin zymography and subsequent computerized densitometry from tumor and nearby stromal tissue and serum from 49 cats with various malignancies. The serum concentrations of MMPs from these tumor-bearing cats were compared with serum concentrations of MMPs from 44 normal cats of similar age and gender. Measurable concentrations of MMPs 2 and 9 were found within tumor, stromal, and serum samples. Mean concentrations of total pro and active MMPs 2 and 9 within tumor tissue were significantly higher (P values <.0001, .0031, <.001, and .0064, respectively) when compared with stromal tissue from the same animals. Serum MMP concentrations from tumor-bearing cats were higher than those from normal cats. Poor correlation was found between serum MMP concentrations and tissue MMP concentrations of increasing histologic grades of malignancies. 相似文献
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Kristi Kabyashree Rahul Kumar Piyali Sen Siddhartha S. Satapathy Suvendra K. Ray 《Plant pathology》2020,69(7):1347-1356
Ralstonia solanacearum causes a lethal bacterial wilt disease in many plants by colonizing the vascular tissues of the hosts. Upon inoculation of tomato seedlings through either leaf or root, the wilting symptoms occur first at the apical region and then proceed downward along the shoot. The systemic order of the disease initiation and progression in the host, independent of the site of pathogen inoculation, is yet to be investigated. To understand the disease progression more clearly, we have carried out a systematic study of the pathogen localization by GUS staining of inoculated tomato seedlings, at 24-hour intervals from 0 days post-inoculation (dpi) to 5 dpi. In both inoculation methods, pathogen colonization was observed at 1 dpi at the apical meristem as well as the cotyledon leaves, where the disease initiates. As the disease progressed, colonization by the pathogen towards the lower region of the shoot was observed. Disease consistency and pathogenicity magnitude were observed to be higher using the leaf inoculation method than the root inoculation method. Several R. solanacearum transposon-induced mutants that were reduced in virulence by root inoculation but virulent by leaf inoculation were obtained. Using GUS staining, it was observed that these mutants were unable to localize in the shoot region when inoculated in the root. Our study indicates that the apical meristem and the cotyledon leaves are the first regions to be colonized in inoculated tomato seedlings, which might explain the disease initiation from this region. 相似文献
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Xiong N Brewer MT Anderson KL Watrous GK Weeks KE Barnhill AE Day TA Kimber MJ Carlson SA 《Veterinary journal (London, England : 1997)》2012,192(3):535-537
This study assessed the capacity of β-lactam antibiotics to prevent salmonella-mediated encephalopathy in calves given the putative neuroprotective effects of these drugs of increasing glutamate export from the brain. Both ampicillin and ceftiofur prevented the development of encephalopathy despite resistance of the inoculated Salmonella enterica serovar Saint-Paul isolate to both drugs. A glutamate receptor antagonist also prevented this salmonella-mediated encephalopathy. Glutamate exporters were hyper-expressed in the presence of β-lactam antibiotics while a glutamate export inhibitor obviated the effects of these antibiotics, demonstrating a neuroprotective effect through glutamate export from the brain. The findings indicate that β-lactam antibiotics remain an important treatment option for this atypical form of bovine salmonellosis. 相似文献
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Mueller RS Fettman MJ Richardson K Hansen RA Miller A Magowitz J Ogilvie GK 《American journal of veterinary research》2005,66(5):868-873
OBJECTIVE: To determine essential fatty acid concentrations in plasma and tissue before and after supplementation with n-3 fatty acids in dogs with atopic dermatitis. ANIMALS: 30 dogs with atopic dermatitis. PROCEDURE: Dogs received supplemental flaxseed oil (200 mg/kg/d), eicosapentaenoic acid (EPA; 50 mg/kg/d)-docosahexaenoic acid (DHA; 35 mg/kg/d), or mineral oil as a placebo in a double-blind, placebo-controlled, randomized trial. Clinical scores and plasma and cutaneous concentrations of linoleic acid, arachidonic acid, alpha-linolenic acid (alpha-LLA), EPA, DHA, prostaglandin E2, and leukotriene B4 were determined. RESULTS: Total plasma concentrations of alpha-LLA and EPA increased and those of arachidonic acid decreased significantly with administration of EPA-DHA, and concentrations of alpha-LLA increased with flaxseed oil supplementation; nevertheless, there was no significant change in the concentrations of these fatty acids or eicosanoids in the skin. There was no correlation between clinical scores and plasma or cutaneous concentrations for any of the measured fatty acids or eicosanoids. CONCLUSION AND CLINICAL RELEVANCE: Results indicated that at the dose used, neither the concentrations of fatty acids in skin or plasma nor a decrease in the production of inflammatory eicosanoids was a major factor involved in the mechanism of action in dogs with atopy that responded to fatty acid supplementation. 相似文献
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Peterson KL MacLeod AG Wisner ER Larson RF Pollard RE 《American journal of veterinary research》2008,69(1):45-50
OBJECTIVE: To determine effects of regional variation, interobserver variability, and vessel selection on quantitative vascular variables derived by dynamic contrast-enhanced computed tomography (DCE-CT) of the brain of clinically normal dogs. ANIMALS: 14 adult dogs with no evidence of CNS dysfunction. PROCEDURES: Dogs were randomly assigned to 4 groups, and DCE-CT was performed at the level of the frontal lobe, rostral portion of the parietal-temporal lobes, caudal portions of the parietal-temporal lobes, or occipital lobe-cerebellum for groups 1 to 4, respectively. Cerebral blood flow (CBF), cerebral blood volume (CBV), and permeability in gray and white matter for both a large and small artery were calculated and compared. Values among 3 observers and 4 regions of the brain were calculated and compared. RESULTS: Significant interobserver variability was detected for CBF and permeability in white matter. Values calculated for large and small arteries were correlated for CBV and CBF but not for permeability. Overall mean +/- SD for CBF, CBV, and permeability in gray matter was 53.5 +/- 27.7 mL/min/100 g, 2.9 +/- 1.4 mL/100 g, and 1.4 +/- 2.2 mL/min/100 g, respectively. Mean for CBF, CBV, and permeability in white matter was 44.2 +/- 28.5 mL/min/100 g, 2.5 +/- 1.5 mL/100 g, and 0.9 +/- 0.7 mL/min/100 g, respectively. Values did not differ significantly among brain regions. CONCLUSIONS AND CLINICAL RELEVANCE: Significant regional variations were not detected for quantitative vascular variables in the brain of clinically normal dogs. However, interobserver variability and vessel selection have an important role in variable estimation. 相似文献
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