首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   2522篇
  免费   149篇
林业   210篇
农学   291篇
基础科学   31篇
  562篇
综合类   123篇
农作物   194篇
水产渔业   300篇
畜牧兽医   683篇
园艺   69篇
植物保护   208篇
  2023年   35篇
  2022年   71篇
  2021年   82篇
  2020年   105篇
  2019年   124篇
  2018年   153篇
  2017年   154篇
  2016年   123篇
  2015年   86篇
  2014年   122篇
  2013年   227篇
  2012年   177篇
  2011年   153篇
  2010年   133篇
  2009年   94篇
  2008年   105篇
  2007年   99篇
  2006年   83篇
  2005年   58篇
  2004年   49篇
  2003年   43篇
  2002年   30篇
  2001年   40篇
  2000年   34篇
  1999年   27篇
  1998年   21篇
  1997年   19篇
  1996年   11篇
  1995年   6篇
  1994年   7篇
  1993年   6篇
  1992年   11篇
  1991年   15篇
  1990年   14篇
  1989年   10篇
  1988年   11篇
  1987年   13篇
  1986年   7篇
  1985年   6篇
  1984年   7篇
  1983年   8篇
  1982年   5篇
  1981年   9篇
  1980年   5篇
  1979年   14篇
  1974年   8篇
  1973年   8篇
  1971年   7篇
  1969年   6篇
  1967年   4篇
排序方式: 共有2671条查询结果,搜索用时 125 毫秒
1.
2.
Manipulated Mouse Embryos as Bioassay System for Water Quality Control   总被引:1,自引:0,他引:1  
Mouse pronuclear stage embryos with intact slit zona pellucida (manipulated) were cultured in vitro until the hatched blastocyst stage in simplex optimized medium with higher K+ concentration (KSOM) prepared with three different water types: tap, deionized reverse osmosis (D‐O) water and Milli‐Q system (M‐Q) water. The culture media were supplemented with or without protein and ethylenediaminetetraacetic acid (EDTA, disodium salt). The rates of hatched blastocysts were significantly affected (p < 0.01) by micromanipulation, protein supplement and water source. The water source has no influence (p > 0.05) on development in EDTA‐supplemented protein‐free culture media, whereas in EDTA‐ and protein‐free culture media, the water quality significantly (p < 0.001) affected the rates of development, with higher rates in media prepared with M‐Q water. The micromanipulated embryos showed higher sensitivity to the water quality (p < 0.01). It worth mentioning that the rates of hatched blastocysts in protein‐free culture media were very low (0–7.5%). Furthermore, the three different water types were analysed by measuring the electrical conductivity, inorganic ions, total organic carbon and endotoxins to evaluate the purity. M‐Q water showed the lowest levels of inorganic ion, total organic carbon and endotoxin concentrations. We concluded that manipulated mouse embryos are good system to evaluate the quality of water used in biological system.  相似文献   
3.
The relative amounts of primary and secondary sulfates in atmospheric aerosols and precipitation can be estimated from measurements of the stable oxygen isotope ratios. The oxygen-18 content of sulfates formed in power plant stack gases before emission into the atmosphere is significantly higher than that of sulfates formed from sulfur dioxide after emission. Results show that 20 to 30 percent of the sulfates in rain and snow at Argonne, Illinois, are of primary origin.  相似文献   
4.
5.
To assess the effect of the angiotensin‐converting enzyme (ACE) inhibition on the efficiency of the fixed‐time artificial insemination (TAI), 69 goats were divided randomly into two groups: enalapril (n = 35) and control (n = 34). In the experiment, all animals underwent the protocol of fixed‐time artificial insemination for 12 days. Enalapril group received enalapril maleate dissolved in saline (Enalapril, Lab Teuto Ltda) subcutaneously at the following doses: 0.2 mg/kg/day in D0‐D2; 0.3 mg/kg/day in D3‐D6 and 0.4 mg/kg/day in D7‐D11. The control group received the corresponding volume of 0.9% saline solution. We performed a single insemination 36 hr after sponge removal using frozen semen from two adult male goats with recognized fertility. The ultrasound pregnancy diagnosis was 30 days after the artificial insemination (AI). There was significant increase in pregnancy rates and twinning as well as a decrease in foetal loss in animals receiving enalapril (p < .01). The use of ACE inhibitors during the TAI protocol was shown to be a promising alternative to increase the efficiency of such reproductive biotechnology.  相似文献   
6.
Trace minerals feeding had significant effects on sperm production and fertility with better absorption and proper utilization within the body for optimum reproductive function. Several studies have shown that more influenced trace elements in the diets of animals are copper (Cu) and zinc (Zn). Bucks showing deficiency of this mineral might affect the quality of semen production which in turn would affect the fertility. This experiment was thus designed to test the effects of organic Cu and Zn supplementation on antioxidants enzyme activities and sperm functional attributes in fresh semen of bucks. Forty bucks (n = 40, Aged 5 months) were assigned to ten groups of four animals in each group, supplemented (for a period of 8 months) with different levels of organic Zn: 20 mg (T2), 40 mg (T3) and 60 mg (T4), organic Cu: 12.5 mg (T5), 25 mg (T6), 37.5 mg (T7) and combined organic Zn and Cu: 20 + 12.5 mg (T8), 40 + 25 mg (T9), 60 + 37.5 mg (T10), respectively, per kg dry matter and no additional mineral diet (control; T1). One hundred and sixty semen samples were collected through electro‐ejaculator and analysed for sperm quantity, quality, acrosome intactness and plasma membrane integrity and correlated with the catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase enzyme activities in seminal plasma. The results indicated organic Cu and zinc supplemented bucks produced more sperm cells, had higher sperm concentrations, maintained higher (< .01) sperm livability, plasma membrane and acrosome integrities, more motility and velocity. The increased antioxidant enzyme activities, reduced oxidative stress and lowered lipid peroxidation were positively correlated (< .05) with the sperm functional attributes. In conclusion, organic Cu and Zn supplement to male goats showed protective roles against oxidative damage and maintained better fresh semen characteristics.  相似文献   
7.
8.
9.
10.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号