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Kumiko TAKEDA Masahiro SATOH Shreeram P. NEOPANE Bahadur S. KUWAR Hari D. JOSHI Nanda P. SHRESTHA Hiroshi FUJISE Mariko TASAI Takahiro TAGAMI Hirofumi HANADA 《Animal Science Journal》2004,75(2):103-110
Dwarf Lulu cattle, the only Bos Taurus type of cattle in Nepal, are raised under severe environments in the mountainous zone of that country. In the present study, the body measurement traits, cytogenetic and molecular genetic characteristics of the Lulu cattle are investigated. Blood samples were collected from 31 animals in four villages (altitudes 2590–3550 m) in the southern part of Mustang. The Lulu cattle had a normal karyotype with 2n = 60, XY or XX. Only one male examined had a large submetacentric X‐chromosome and a small submetacentric taurine type Y‐chromosome. The mitochodrial DNA (mtDNA) genotypes were analyzed by PCR mediated restriction fragment length polymorphisms, displacement (D)‐loop region PCR mediated single strand conformation polymorphisms, and D‐loop region sequences. Many base substitutions were found in the D‐loop region, suggesting that the Lulu cattle originated from at least 10 maternal lines. Three types of mtDNA from these cattle were found, the Bos taurus type (n = 23), the Bos indicus type (n = 6), and the Bos grunniens type (n = 2). In the village at the lowest altitude, four of the five cows were of the Bos indicus type. These results indicated that mtDNA types of the Lulu cattle mostly belong to Bos taurus, but have been hybridized with Bos indicus cattle in lower‐elevation regions in their maternal lineage. 相似文献
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Kanae SHIOKAWA Chandika D. GAMAGE Nobuo KOIZUMI Yoshihiro SAKODA Kenta SHIMIZU Yoshimi TSUDA Kumiko YOSHIMATSU Jiro ARIKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):221-230
The applicability of the recombinant LipL32 for serodiagnosis of leptospiral infection in field rodents was
assessed in this study. An immunodominant region of LipL32 was determined by monoclonal antibodies, and then,
truncated LipL32 (tLipL32) was designed to contain the region (87–188th amino acid). The tLipL32 was compared
between two recombinant expression hosts Escherichia coli and Pichia
pastoris in ELISA. With field rat sera, tLipL32 expressed by P. pastoris
(tLipL32p) had high antigenicity without background reactions, while tLipL32 expressed by E.
coli (tLipL32e) showed high background reactions, which were reduced by pre-adsorption of sera with
E. coli. To evaluate tLipL32-ELISA, field rat sera were tentatively divided into a
Leptospira infection positive (12 sera) and a negative group (12 sera) based on the results
from flaB gene PCR of kidney samples and WB with whole Leptospira cell.
Consequently, the sensitivity of tLipL32p-ELISA for field rat sera was 83% . A similar result was obtained
from tLipL32e-ELISA with adsorbed sera, (92%). However, sensitivity of tLipL32e-ELISA using sera without an
adsorption treatment was 50%. Regardless of the expression host, tLipL32-ELISA had 100% specificity and
sensitivity in experimentally infected laboratory rats. These results suggest that recombinant LipL32
expressed by P. pastoris is more applicable for serodiagnosis in field rats due to a lack of
background reaction. 相似文献
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Okabayashi K Kano R Sirouzu K Yanai T Mizuno M Yamamura H Saegusa S Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(8):953-955
Two feline cases were diagnosed as systemic cryptococcosis due to Cryptococcus neoformans (teleomorph: Filobasidiella neoformans) by PCR assay with CAP59 gene primers using urine, serum and biopsy samples. The results of molecular analysis were consistent with the mycological findings. 相似文献
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Saito K Nakanishi M Hasegawa A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(6):495-497
The type, ages of occurrence, primary complaints, clinical signs and mortality in forty-seven cases of uterine disorders diagnosed by ventrotomy in rabbits were analyzed retrospectively. Endometrial hyperplasia (29.8%) was most frequently observed, followed by uterine adenocarcinoma (21.3%). Tumorous lesions were seen in 46.8% of the cases. The age of occurrence ranged from two years and two months to seven years and six months, with a peak at four to five years of age. The most common primary complaint was bleeding (62.2%), followed by mammary gland abnormality (12.8%) and increased abdominal circumference (10.6%). Physical examinations revealed mammary gland disorders such as mammary cysts in 31.9% of the cases. Uterine disorders were detected by palpation in 15 out of 32 cases with a primary complaint of bleeding. Ultrasonography showed uterine disorders in 21 out of 24 cases, suggesting that ultrasonography could be useful in the diagnosis of uterine disorders. The outcome seemed to be influenced by physical status rather than malignancy of lesions. The mortality was higher in cases with symptoms such as anorexia, emaciation, severe anemia, and dehydration. 相似文献
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Kumiko Oka Akiko Okubo Motoichiro Kodama Hiroshi Otani 《Journal of General Plant Pathology》2006,72(3):152-158
In tomato plants, α-tomatine, a steroidal glycoalkaloid saponin, inhibits fungal growth. Tomato pathogens that produce host-specific
toxins, Alternaria alternata tomato pathotype causing Alternaria stem canker and Corynespora cassiicola causing Corynespora target spot, were investigated for sensitivity to α-tomatine. Although spore germination of A. alternata pathogenic and nonpathogenic to tomato and of C. cassiicola pathogenic to tomato was not affected by 0.1 mM α-tomatine, spore germination of C. cassiicola nonpathogenic to tomato was significantly inhibited. This result showed that A. alternata, regardless of its pathogenicity, and only the C. cassiicola pathogenic to tomato are resistant to α-tomatine. Germinating spores of A. alternata and C. cassiicola resistant to α-tomatine detoxified α-tomatine by degrading it to a less polar product. After inoculation of tomato leaves,
spores of A. alternata and C. cassiicola nonpathogenic to tomato germinated and formed appressoria, but did not form infection hyphae in host tissues. When a host-specific
toxin (CCT-toxin) produced by C. cassiicola pathogenic to tomato was added to nonpathogenic spores, colonization within leaves was observed in A. alternata, but not in C. cassiicola. On the other hand, when spores of C. cassiicola nonpathogenic to tomato were suspended in spore germination fluid of nonpathogenic A. alternata with α-tomatine detoxification activity, the fungus could be induced to colonize leaves in the presence of CCT-toxin. These
results indicate that A. alternata tomato pathotype and C. cassiicola pathogenic to tomato detoxify α-tomatine during infection and that this detoxification is essential for host colonization
by pathogens that produce host-specific toxins. 相似文献
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Fumiko SUZUTA Kumiko KIMURA Ryo URAKAWA Yukio KUSUDA Shogo TANAKA Yasuko HANAFUSA Makoto HARITANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):1029-1031
This report presents a new case of mucormycosis encountered in penguin characterized by morphological variation of hyphae and presence of sporangia with numerous sporangiospores. A 4.5-year-old Magellanic penguin (Spheniscus magellanicus) died after exhibiting anorexia, poor nutritional condition and dyspnea. Multiple nodular lesions were observed in the thoracic and abdominal regions. Histopathologically, hyphae of various sizes were seen in the lungs, air sac and nodular lesions. Myriad sporangiospores and several sporangia were observed in/around the bronchi or parabronchi. The very narrow and short hyphae in the nodules were not consistent with the characteristics of Mucorales. However, for most hyphae, including those in the nodules, sporangiospores and sporangia, immunohistochemistry revealed Mucorales-positive reactions. In addition, these fungi were identified as Rhizomucor pusillus by gene
analysis. 相似文献
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This study was performed to obtain information on the occurrence of multiple paternities in three species of viviparous Japanese
surfperch using allelic markers of microsatellite DNA loci. Direct evidence for multiple fertilizations was established by
reconstructing paternal genotypes from the progeny of gravid females. Multiple paternities were ascertained in five of 10
broods of Ditrema temmincki and in three of nine broods of Neoditrema ransonneti, but not in Ditrema viride. The number of patrilines detected in the progeny of D. temmincki and N. ransonneti females were two or three, respectively, as determined by the GERUD v2.0 algorithm for reconstructing parental genotypes
from half-sib progeny arrays. 相似文献