哈茨木霉对水稻恶苗病菌的拮抗作用   总被引：11，自引：0，他引：11       下载免费PDF全文

产祝龙  丁克坚  檀根甲  苏贤岩  朱圣杰  陈琪 《植物保护》2003,29(3):35-39

PDA平板拮抗试验表明 ,哈茨木霉对水稻恶苗病菌有强烈的拮抗作用 ,其孢子悬浮液的含孢量为 10⁶～10⁷个 /mL时 ,对恶苗病菌的抑制力达 92.33%。通过哈茨木霉菌液和 3种药剂对水稻恶苗病菌抑制效果的比较 ,哈茨木霉孢子悬浮液含孢量为 10⁷个 /mL与施保克质量浓度 1μg/mL的抑菌效果接近 ,分别为 76.7%、75.4%。显微摄影结果显示 ,哈茨木霉以附着胞附着在恶苗病菌菌丝上 ,然后穿透菌丝在其内生长 ,或与恶苗病菌的菌丝平行生长 ,然后再侵入病菌内寄生。  相似文献   

变风量空调系统控制优化方法研究     

陈友明  刘健  程时柏  陈永康  秦建英  周强 《湖南农业大学学报(自然科学版)》2013,40(6):1-6

提出了“总风量-阀位控制法”和“最大负荷率-最小风量法”分别优化变风量空调系统送风静压和送风温度，并应用到一座实际建筑的变风量空调系统中.建立了变风量空调风系统模型，利用TRNSYS对大楼典型层风系统进行模拟，用实测数据验证模型正确性，模拟分析了典型日变风量系统优化前后的风机能耗.模拟结果表明，与定静压定送风温度的原控制方法相比，优化控制能节约风机能耗，在部分负荷下效果尤为明显，冬季和过渡季试验日的节能率分别为19.38%和15.58%.对2种控制方法下的全年能耗和室内热舒适性进行了试验对比测试，结果表明，全年的节能率为7.16%，而且室内热舒适性得到显著提高.  相似文献   

Safeguarding Capability Index System of Regional Sustainable Development     

WU Ying  LIN Yong  CHAN Qi-bing 《保鲜与加工》2006,(2):155-158

According to the new requirement of sustainable development in the new stage, this paper puts forward the definition of the safeguarding capacity of regional sustainable development and uses some statistical methods of frequency statistics and principal component analysis to construct the index system of safeguarding capacity. Then it takes the data of the Three Gorges reservoir area for example to make empirical analysis, and provides efficient measurement tools to further measure the regional sustainability together with its safeguarding capacity, and therefore helps the government and the relevant organizations to establish more scientific policies of sustainable development.  相似文献   

挖掘机主油泵工作异常现象的原因探究     

单艳芬 《湖南农机》2017,44(2)

挖掘机主油泵是主要的能量转换部件,它的好坏直接影响着车辆的正常工作.文章从挖掘机主油泵的结构组成、工作原理,逐渐探究挖掘机主油泵工作异常的检查方法.最后,文章以挖掘机主油泵油封漏油故障现象,理清思路,逐步分析探究出最后故障原因.  相似文献   

Suppression of influenza virus infection by the orf virus isolated in Taiwan     

Fong-Yuan LIN  Yeu-Yang TSENG  Kun-Wei CHAN  Shu-Ting KUO  Cheng-Hsiung YANG  Chi-Young WANG  Masaki TAKASU  Wei-Li HSU  Min-Liang WONG 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1055-1062

Orf virus (ORFV), a member of parapoxvirus, is an enveloped virus with genome of double-stranded DNA. ORFV causes contagious pustular dermatitis or contagious ecthyma in sheep and goats worldwide. In general, detection of viral DNA and observing ORFV virion in tissues of afflicted animals are two methods commonly used for diagnosis of orf infection; however, isolation of the ORFV in cell culture using virus-containing tissue as inoculum is known to be difficult. In this work, the ORFV (Hoping strain) isolated in central Taiwan was successfully grown in cell culture. We further examined the biochemical characteristic of our isolate, including viral genotyping, viral mRNA and protein expression. By electron microscopy, one unique form of viral particle from ORFV infected cellular lysate was demonstrated in the negative-stained field. Moreover, immunomodulating and anti-influenza virus properties of this ORFV were investigated. ORFV stimulated human monocytes (THP-1) secreting proinflammatory cytokines IL-8 and TNF-α. And, pre-treatment of ORFV-infected cell medium prevents A549 cells from subsequent type A influenza virus (IAV) infection. Similarly, mice infected with ORFV via both intramuscular and subcutaneous routes at two days prior to IAV infection significantly decreased the replication of IAV. In summary, the results of a current study indicated our Hoping strain harbors the immune modulator property; with such a bio-adjuvanticity, we further proved that pre-exposure of ORFV protects animals from subsequent IAV infection.  相似文献   

Hatchery production of Florida red tilapia seed in brackishwater tanks: the influence of broodstock age     

S. J. SMITH  W. O. WATANABE  J. R. CHAN  D. H. ERNST  R. I. WICKLUND  B. L. OLLA 《Aquaculture Research》1991,22(2):141-147

Abstract. Production of Florida red tilapia seed (eggs, sacfry and fry) in 34-m² above-ground tanks using recirculated brackishwater (12 ppt) was studied over a 5-month period on Lee Stocking Island (Exuma Cays, Bahamas). Four tanks were stocked with year class 1 (YCI) breeders, while 2 tanks were stocked with year class 2 (YCII) breeders at densities of 200 and 240/tank (5·9 and 7·1/m²), respectively, and at a sex ratio of 3 females to 1 male, beginning in November 1988. Seed production was measured by removing free-swimming fry from tanks and eggs and sacfry from the mouths of brooding females, and assessing the number of each at 15 to 16-day intervals from February to June 1989. Average seed production (seed/m²/day) was significantly higher in YCI (52·3) than in YCII (36·0) broodstock, indicating the advantages of replacing broodstock with yearling breeders each year.  相似文献   

水稻恶苗病菌对使百克、多菌灵的抗性遗传研究   总被引：4，自引：0，他引：4  

赵淑元  产祝龙  丁克坚 《安徽农业大学学报》2008,35(1):46-48

采用使百克、多菌灵对病原菌直接进行诱变,获得相应的抗性菌株,对其抗性遗传规律进行研究.结果表明,安徽省水稻恶苗病菌对多菌灵已产生了中等程度的抗药性,对使百克的抗性较低.通过多菌灵、使百克诱变均都能产生抗性菌株,但就抗性增长速度而言,多菌灵远高于使百克.产生抗性的突变菌株后代表现说明,多菌灵的抗性突变菌株遗传较稳定.单剂诱变的抗性菌株致病性高于复配剂诱变菌株和敏感菌株.  相似文献   

论多校区大学学生党建工作   总被引：2，自引：0，他引：2  

严冰  产佳 《河北农业大学学报(农林教育版)》2006,8(3):24-27

近年来,高校多校区办学已成为全国高校普遍的运作方式,这给我国传统的大学运作管理格局带来了许多全新的冲击与挑战,其中,学生党建工作尤为突出。本文通过对现阶段多校区高校党建工作面临的问题与困难展开分析,在此基础上提出了建立健全各类党建制度、充分利用网络资源并在学生公寓开展党建工作等相应对策。  相似文献   

Changes in plasma biochemistry in horses competing in a 160 km endurance ride     

R. J. ROSE  D. R. HODGSON  D. SAMPSON  W. CHAN 《Australian veterinary journal》1983,60(4):101-105

SUMMARY Plasma biochemical values were measured in 14 horses, before a 160 km endurance ride, immediately after 85 km, immediately after 160 km, after 30 min recovery period and the day after the ride. For statistical analysis, a group of 7 horses that completed the ride at a mean speed of 234 metres per min (m/min) (Fast Group) was compared with 7 horses that completed the ride at a mean speed of 144 m/min (Slow Group). Estimations were made of sodium, potassium, chloride, bicarbonate, glucose, creatinine, urea, bilirubin, iron, total protein, albumin, calcium, phosphate, cholesterol, alkaline phosphate, gammaglutamyl transferase, lactate dehydrogenase, a hydroxybutyrate dehydrogenase, asparate amino transferase, alanine amino transferase, Cortisol and insulin. Disturbances in boichemical values associated with endurance exercise reflected dehydration, hepatic dysfunction, reduced renal function and muscle damage. The faster the speed of exercise, the greater was the stress as reflected by Cortisol values. The Fast Group of horses also demonstrated lower glucose and higher CK and LD values than the Stow Group of horses. However, biochemical values the day after the ride were similar to values before the ride.  相似文献   

COⅠ和16S rRNA基因在高原裂腹鱼物种鉴定中的应用     

产久林  姜华鹏  刘一萌  胡星星  王丛丛  许强华 《水生态学杂志》2015,36(4):98-104

针对裂腹鱼种类繁多、种间形态学差异不明显的特点,利用分子标记对高原裂腹鱼进行准确的物种鉴定具有重要价值。对采集的60尾高原裂腹鱼进行了COⅠ和16S rRNA基因的部分序列测定,并通过GenBank数据库进行鱼种鉴定;运用邻接法（NJ）构建系统发育树,对实验鱼进行群系划分;通过DNASP软件分析比较实验鱼COⅠ和16S rRNA基因的序列变异;依据MEGA 6.0的Kimura-2-parameter模型计算实验鱼的种内遗传距离和种间遗传距离,并对COⅠ和16S rRNA基因在高原裂腹鱼物种鉴定中的适用性进行探讨。结果表明,采集的样本包含3属、5种裂腹鱼,分别为尖裸鲤属的尖裸鲤（Oxygymnocypris stewartii）、裸鲤属的软刺裸鲤（Gymnocypris dobula）、裂腹鱼属的拉萨裂腹鱼（Schizothorax waltoni）、巨须裂腹鱼（Schizothorax macropogon）和异齿裂腹鱼（Schizothorax oconnori）。在COⅠ基因构建的系统发育树中,5种高原裂腹鱼均形成独立的一支,而16S rRNA基因不能准确地对裂腹鱼属的3个鱼种实现区分。研究显示,5种高原裂腹鱼COⅠ基因核苷酸的变异水平（Pi）和单倍型多样性指数（Hd）均高于16S rRNA基因;COⅠ基因计算得到实验鱼种间遗传距离和种内遗传距离平均值分别为0.025和0.002,种内和种间的最大遗传差异约为13倍;16S rRNA基因得出结果为0.026和0.005,而种内和种间的最大遗传差异约为6倍。综合COⅠ和16S rRNA基因能有效鉴定高原裂腹鱼物种,而在单个基因的选择上,COⅠ基因具有更高的适用性。  相似文献