SPLENIC SEQUESTRATION SCINTIGRAPHY IN THE DOG: A COMPARISON OF DENATURING TECHNIQUES     

Clifford R.  Berry  DVM  John H.  Kuperus  PhD  Daniel  Malone  BA 《Veterinary radiology & ultrasound》1995,36(1):57-63

Five mixed-breed dogs underwent splenic sequestration scintigraphy following intravenous injection of 647.5 to 740 MBq (17.5–20 mCi) of ^99mTechnetium labeled autologous red blood cells (RBCs) that had been chemically denatured using two μg of stannous chloride. Left lateral dynamic images were obtained for 20 minutes after injection. Regions of interest (ROI) were drawn around the splenic body and ventral extremity, ventral liver and caudal abdominal great vessels and time activity curves created. Count density information was obtained and uptake ratios were calculated for the spleen ROI/vessel ROI, liver ROI/vessel ROI and spleen ROI/liver ROI at 1, 2.5, 5, 10, 15 and 20 minutes after injection. Two additional studies using different RBC denaturing procedures were done in four of the five dogs. In the second study, the stannous chloride level was doubled to 4.0 μg, while in the third study, the RBCs were denatured by addition of 2.0 μg of stannous chloride and heating at 49°C for 15 minutes. Progressive splenic uptake of denatured RBCs was seen in all dogs during the first 15 to 20 minutes of each study, no matter which denaturing technique was utilized. Significant increases in the spleen ROI:/vessel ROI and spleen ROI:liver ROI ratios were obtained at 5, 10, 15 and 20 minutes when compared to the 1 minute values for each of the labeling techniques. A significant difference was not identified between the three ratios at 15 minutes between the three labeling techniques. There was a trend of increasing values for each ratio where 2.0 μg of stannous chloride technique being the lowest, 4.0 μg of stannous chloride having an intermediate value and the heated technique had the highest values. The heating technique resulted in higher liver activity and increased variability of the mean liver ROI/vessel ROI ratios at all times (1, 2.5, 5,10, and 15 minutes). Using a non-linear least squares regression analysis, a double exponential equation fit the spleen ROI/vessel ROI and spleen ROI/liver ROI ratios for all three labeling techniques. Imaging of the spleen using all three techniques was acceptable, and a persistent blood pool image would allow for vascular imaging and cardiac gated studies 30 minutes after injection of the denatured, labeled autologous red blood cells. Veterinary Radiology & Ultrasound, Vol. 36, No. I, 1995, pp 57–63.  相似文献   

COMPARATIVE STUDY OF INDIUM-111 LEUKOCYTES AND NEPHROSONOGRAPHY FOR DETECTION OF EXPERIMENTAL PYELONEPHRITIS IN DOGS     

L. Neuwirth  DVM  MS  J.H. Kuperus  PhD  M. Calderwood-Mays    DVM  PhD  J. Gaskin  DVM  PhD 《Veterinary radiology & ultrasound》1995,36(3):253-258

Nephrosonography was compared to In-111 WBC for detection of experimentally induced pyelonephritis in 12 dogs. Nephrosonography was performed during the acute and chronic phase of pyelonephritis in all dogs. Sonographic abnormalities were found in all infected kidneys imaged early after infection. In the chronic phase of pyelonephritis, the sonographic abnormalities had decreased in severity in 9 kidneys, increased in severity in 2 kidneys, and had resolved in 2 kidneys. After histopathologic evaluation, eleven of fifteen kidneys (73%) with pyelonephritis had been identified by sonography. The In-111 WBC scan was performed during the acute phase of pyelonephritis in 9 dogs and during the chronic phase in all dogs. During the acute phase of pyelonephritis, the WBC scan was positive in 8 of the 9 dogs (89%) on images acquired 24 and 48 hr after injection of the labelled WBC. During the chronic phase of pyelonephritis, the WBC scan was negative in all 12 dogs. The sensitivity of the In-111 WBC scan was poor compared to nephrosonography for detection of chronic pyelonephritis. In this study, optimal detection of renal infection/pyelonephritis by In-111 WBC may be dependent on the presence of clinical signs and leukocytosis in dogs. Even when leukocytosis is present, the In-111 WBC may fail to accumulate in the kidney in dogs with pyelonephritis, particularly if no clinical signs (fever, depression, inappetence) are present.  相似文献