Sequence and unique phylogeny of G genes of bovine respiratory syncytial viruses circulating in Japan     

Asuka Kumagai  Kyoko Kawauchi  Kiyohiko Andoh  Shinichi Hatama 《Journal of veterinary diagnostic investigation》2021,33(1):162

Bovine respiratory syncytial virus (BRSV) is an etiologic agent of bovine respiratory disease. The rapid evolutionary rate of BRSV contributes to genetic and antigenic heterogeneity of field strains and causes occasional vaccine failure. We conducted molecular epidemiologic characterization of BRSV circulating in Japan to obtain genetic information for vaccine-based disease control. Phylogenetic analysis of G and F gene sequences revealed that all of the isolated Japanese BRSV strains clustered in the same genetic subgroup, which was distinct from the 9 known groups. We assigned the Japanese group to subgenotype X. The Japanese isolates formed 2 temporal clusters: isolates from 2003 to 2005 clustered in lineage A; isolates from 2017 to 2019 formed lineage B. The alignment of the deduced amino acid sequences of the G gene revealed that the central hydrophobic region responsible for viral antigenicity is conserved in all of the isolates; unique amino acid mutations were found mainly in mucin-like regions. Our results suggest that BRSV has evolved uniquely in Japan to form the new subgenotype X; the antigenic homogeneity of the viruses within this group is inferred.  相似文献   

Identification of the core rumen bacterial taxa and their population dynamics during the fattening period in Japanese Black cattle     

Hiroto Miura  Takuya Hashimoto  Yukiko Kawanishi  Hiroki Kawauchi  Ryo Inoue  Noriaki Shoji  Kunihiko Saito  Mario Sekiya  Yosuke Saito  Jumpei Yasuda  Chiemi Yonezawa  Tetsushiro Endo  Hirotaka Kasuya  Yutaka Suzuki  Yasuo Kobayashi  Satoshi Koike 《Animal Science Journal》2021,92(1):e13601

The rumen microbiota comprises a vast range of bacterial taxa, which may affect the production of high-quality meat in Japanese Black cattle. The aim of this study was to identify core rumen microbiota in rumen fluid samples collected from 74 Japanese Black cattle raised under different dietary conditions using 16S rRNA gene amplicon sequencing. In the rumen of fattening Japanese Black cattle, 10 bacterial taxa, showing >1% average relative abundance and >95% prevalence, irrespective of the dietary conditions and the fattening periods, were identified as the core rumen bacterial taxa, which accounted for approximately 80% of the rumen microbiota in Japanese Black cattle. Additionally, population dynamics of the core rumen bacterial taxa revealed two distinct patterns: Prevotella spp. and unclassified Bacteroidales decreased in the mid-fattening period, whereas unclassified Clostridiales, unclassified Ruminococcaceae, Ruminococcus spp., and unclassified Christensenellaceae increased during the same period. Therefore, the present study reports the wide distribution of the core rumen bacterial taxa in Japanese Black cattle, and the complementary nature of the population dynamics of these core taxa, which may ensure stable rumen fermentation during the fattening period.  相似文献   

Isolation of myxoviruses from migratory waterfowls in San-in district,western Japan in winters of 1997-2000     

Shengqing Y  Shinya K  Otsuki K  Ito H  Ito T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(11):1049-1052

Between November 1997 and February 2000, winter migratory waterfowls of several species staying in San-in district, western Japan were surveyed for influenza A virus and paramyxovirus at four stations. A total of 18 influenza A viruses was isolated from 1,404 fecal samples of whistling swans, pintails, mallards, and white-fronted geese. Five different hemagglutinins and eight neuraminidases were identified in the viruses isolated, in 11 different combinations, including H7N8 related to a subtype of a highly pathogenic chicken virus. In 2000, five lentogenic (non-pathogenic) Newcastle disease viruses were also isolated from white-fronted geese. These results suggested that possible precursor viruses for highly pathogenic avian myxoviruses are still brought into Japan by migratory waterfowls. The results also support the contention that continued surveillance of wild waterfowl population should be an integral part of control policies for these serious poultry diseases.  相似文献   

Erratum     

Haruhiko Yamaguchi  Kyoko Yoshino  Akiko Kido 《Journal of Wood Science》2002,48(5):455

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Simple method for synthesizing phenolicβ-O-4 dilignols     

Shingo Kawai  Kyoko Okita  Kazuhiro Sugishita  Ai Tanaka  Hideo Ohash 《Journal of Wood Science》1999,45(5):440-443

A modified synthetic method for phenolic-O-4 lignin substructure model dimers was developed involving protection of the phenolic hydroxyl group of acetophenons with benzoyl chloride, bromination with 4-dimethylaminopyridiniumbromide perbromide, condensation with phenols in the presence of 18-crown-6-ether, condensation with paraformaldehyde, reduction with NaBH₄ and debenzoylation. This method results in shorter reaction times and increasing yields without the application of strict anhydrous and drastic conditions or chloric solvents. This alternative route could be applied to the-O-4 dilignol syntheses of four combinations of guaiacyl and syringyl derivatives.  相似文献   

Nickel-catalyzed carbonization of wood for coproduction of functional carbon and fluid fuels I: production of crystallized mesoporous carbon     

Tsutomu Suzuki  Kyoko Suzuki  Yukio Takahashi  Mitsuhiro Okimoto  Tetsuo Yamada  Noriyasu Okazaki  Yuichi Shimizu  Masashi Fujiwara 《Journal of Wood Science》2007,53(1):54-60

Japanese larch wood loaded with nickel (1%–4%) alone or with nickel and calcium (0.25%–1.5%) was carbonized at 800°–900°C for 0–120min with a heating rate of 5°–20°C min⁻¹ in a helium flow of 5.8−46.4 ml STP cm⁻² min⁻¹ to examine the influence of these variables on the crystallization of carbon (the formation of T component) and the development of mesoporosity. From the obtained results, reaction conditions suitable for effective production of carbon with the dual functions of adequate electroconductivity and adsorption capacity in liquid phase were established, thereby explaining the factors that govern the process. It was also confirmed that mesopore having a diameter of about 4 nm was selectively produced at the cost of specific (BET) surface area in parallel with the formation of T component. This result provided good insight into how the simultaneous dual function could be realized.  相似文献   

Effects of current-year and previous-year PPFDs on shoot gross morphology and leaf properties in Fagus japonica     

Kimura K  Ishida A  Uemura A  Matsumoto Y  Terashima I 《Tree physiology》1998,18(7):459-466

We investigated how shoot gross morphology and leaf properties are determined in Fagus japonica Maxim., a deciduous species with flush-type shoot phenology, in which all leaves are produced in a single flush at the start of each season. We examined relationships between current-year shoot properties and local light environment in a 14-m tall beech tree growing in a deciduous forest. Leaf number (LN), total leaf area (TLA), and total leaf length (SL) of the current-year shoot increased with increasing photosynthetic photon flux density (PPFD). Leaf thickness, dry mass per leaf area and nitrogen content on a leaf area basis increased, whereas the chlorophyll/N ratio decreased with increasing PPFD. To separate the effects of current-year PPFD from those of previous year(s), we artificially shaded a part of the uppermost leaf tier. Reciprocal transfers of beech seedlings between controlled PPFD regimes were also made. Characteristics of shoot gross morphology such as LN, TLA and SL were largely determined by the PPFD of the previous year. The exception was the length of the longest "long shoots" with many leaves, in which elongation appeared to be influenced by both previous-year and current-year PPFD. In contrast, leaf properties were determined by current-year PPFD. The ecological implications of our findings are discussed.  相似文献   

The duality of teleost gonadotropins   总被引：5，自引：0，他引：5  

Hiroshi Kawauchi  Kunimasa Suzuki  Hiromichi Itoh  Penny Swanson  Nobuko Naito  Yoshitaka Nagahama  Masumi Nozaki  Yasumitu Nakai  Seiga Itoh 《Fish physiology and biochemistry》1989,7(1-6):29-38

The duality of salmon gonadotropins has been proved by biochemical, biological, and immunological characterization of two chemically distinc gonadotropins. GTH I and GTH II were equipotent in stimulating estradiol production, whereas GTH II appears to be more potent in stimulating maturational steroid synthesis. The ratio of plasma levels and pituitary contents of GTHs and the secretory control by a GnRH suggest that GTH I is the predominant GTH during vitellogenesis and early stages of spermatogenesis in salmonids, whereas GTH II is predominant at the time of spermiation and ovulation. GTH I and GTH II are found in distinctly separate cells. In trout, GTH I is expressed first in ontogeny, whereas GTH II cells appear coincident with the onset of spermatogenesis and vitellogenesis, and increase dramatically at the time of final reproductive maturation. Comparison of the amino acid sequences of polypeptides and the base sequences of cDNA revealed that salmon GTH I β is more similar to bovine FSHβ than bovine LHβ and salmon GTH II β shows higher homology to bovine LHβ than to bovine FSHβ. The existence of two pituitary gonadotropins in teleosts as well as tetrapods suggests that the divergence of the GTH gene took place earlier than the time of divergence of teleosts from the main line of evolution leading to tetrapods.  相似文献   

New Visible Endotracheal Intubation Method Using the Endoscope System for Mice Inhalational Anesthesia     

Kenjiro KONNO  Naoki ITANO  Teppei OGAWA  Mika HATAKEYAMA  Kyoko SHIOYA  Noriyuki KASAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(6):863-868

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Improvement of Preimplantation Development of In Vitro-Fertilized Bovine Zygotes by Glucose Supplementation to a Chemically Defined Medium     

Nobutada SAKAGAMI  Osamu NISHINO  Satoshi ADACHI  Hidenobu UMEKI  Hiroko UCHIYAMA  Kyoko ICHIKAWA  Kazuhisa TAKESHITA  Etsushi KANEKO  Kiyoshi AKIYAMA  Shuji KOBAYASHI  Hiromichi TAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(10):1403-1405

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