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微管、微丝特异性抑制剂处理对水稻抗病性的影响 总被引：3，自引：0，他引：3
微管特异性抑制剂oryzalin、微丝特异性抑制剂细胞松弛素A (cytochalasin A,CA)和细胞松弛素D (cytochalasin D,CD)的试验表明:oryzalin在5~50μmol/L、CA在0.5~1.0μg/mL、CD在1~20μg/mL的浓度范围内,对稻瘟病菌孢子的萌发和附着胞的形成基本上没有影响。采用以上几种细胞骨架特异性抑制剂处理水稻叶鞘都可以不同程度地抑制寄主细胞抗病菌扩展的能力。在抑制剂处理的水稻叶鞘细胞中,病菌扩展的速度加快。进一步的观察发现,抑制剂处理抑制水稻细胞抗病菌的扩展能力与水稻的抗病防卫反应如原生质颗粒化、多酚类物质的积累和HR发生的延迟是相关的。 相似文献
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Abstract Following an outbreak of botulism in the fish stock of a British trout farm in 1980, a survey of the residual contamination of the farm was carried out. Commencing in November 1980, the farm was examined monthly for one year after the disease had been eliminated. A nearby unaffected farm was examined as a control. Examination of pond muds showed that the affected farm remained contaminated throughout the year, counts of Clostridium botulinum type E ranging from 1-800/g compared with counts at the control farm of 0.1-0.7/g. Both C. botulinum types B and E were found in the control farm, either singly or together in individual samples of pond mud. Fish taken from the affected farm in May and August of 1981 showed a 77.1 % (s.e. ±7.7) contamination by type E. Examination of the environs of the affected farm yielded C. botulinum in 36 of 60 samples of mud and soil using enrichment culture techniques. Clostridium botulinum type E predominated in muds, occurring in 26 of 28 samples taken from the waters supplying and by-passing the farm. In contrast, of the 32 soil samples tested from around the farm, eight yielded type B whereas only three contained type E. 相似文献
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水稻受稻瘟病菌侵染后过氧化物酶定位的超微观察 总被引：10，自引：1，他引：10
利用联苯胺蓝（3，3'-diaminobenzidine, DAB）染色法原位检测了水稻 稻瘟病菌互作过程中H2O2和过氧化物酶被诱导产生和积累的过程。结果表明在病原菌接种后，水稻叶鞘内表皮细胞在伤口、气孔保卫细胞及病菌侵染点等3种情况下可以检测到染色反应。在水稻-稻瘟病菌非亲和性互作中，H2O2产生和过氧化物酶活性上升快，并逐渐积累到较高的水平；而在亲和性互作反应中，H2O2产生和过氧化物酶活性上升被延迟，积累水平较低。超微结构研究表明，在非亲和性互作反应中，过氧化物酶主要定位于被侵染寄主细胞的细胞壁、细胞质、细胞膜、侵染菌丝周围及由膜系统构成的囊泡膜上；而在亲和性互作反应中，早期（接种后16 h）几乎难以观察到过氧化物酶的聚集，后期（接种后30 h）过氧化物酶聚集增多，但仍明显低于非亲和性互作反应。 相似文献
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D. A. FRENCH DVM MVSC S. M. BARBER DVM DiplomateACVS D. H. LEACH PhD C. E. Doige DVM PhD 《Veterinary surgery : VS》1989,18(4):312-321
Arthroscopic surgery was performed on 12 horses (2-4 years of age) to create a 7 x 14 mm full-thickness cartilage defect in one radial carpal bone and in the contralateral third carpal bone. Six horses remained confined to a small paddock and six horses underwent a program of increasing exercise consisting of walking, trotting, and cantering for 13 weeks. All lesions showed evidence of healing at week 6 that progressed to more complete healing at week 13. There was no difference in the amount of repair tissue covering the defect. Histologically, the lesions healed with a combination of fibrous tissue and fibrocartilage. The repair tissue was significantly thicker in the exercised horses but there was no difference in repair quality. It was concluded that radial carpal and third carpal lesions have an equal ability to heal and that early postoperative exercise is not detrimental to the repair tissue within these carpal cartilage defects. 相似文献
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Infection by Erysiphe polygoni was followed on the components of five mixed populations containing different proportions of a partially resistant swede variety, Ruta Øtofte (RØ), and a highly susceptible variety, Ne Plus Ultra (NPU) (100:0; 75:25; 50:50; 25:75; 0:100). Disease levels remained much lower on RØ than on NPU in all populations. For several weeks, disease on NPU was greatest in pure plots and was progressively reduced in mixtures with increasing proportions of RØ. Later, disease levels on NPU were similar in all populations. Disease on RØ was not increased in mixtures with NPU. Root yield of NPU, but not of RØ, was greater in mixtures than in pure stands. The possible use of variety mixtures to control powdery mildew in swede crops is discussed. 相似文献
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Comparative Aspects of the Healing of Excisional Wounds on the Leg and Body of Horses 总被引：1，自引：0，他引：1
K. A. JACOBS BVSc MVSc MACVSc D. H. LEACH MSc PhD P. B. FRETZ VMD DiplomatEACVS H. G. G. TOWNSEND DVM MSC 《Veterinary surgery : VS》1984,13(2):83-90
Standardized 20 mm diameter excisional skin wounds were made on the body and distal limbs of 6 ponies at 18,12, 4, and 2 weeks prior to biopsy. The results of comparative area analysis of leg and body wounds show that leg wounds take significantly longer to heal than body wounds. There is a longer preparatory phase and a greater amount of wound retraction in the leg wounds. At 12 weeks postwounding, the area of the epithelium covering the wound site is significantly larger in the leg wounds compared to the flank wounds. Histological examination of the wounds showed that there is less epithelialization of leg wounds at 2 and 4 week postwounding, and that the granulation tissue of these wounds extends slightly above the level of the adjacent skin. Within the granulation tissue of leg wounds, there are clefts filled with fibrin and inflammatory cells. Once epithelialization is completed, the leg wounds are covered by acanthotic epidermis with numerous large epidermal projections. In contrast, the flank wounds are covered by thin epidermis devoid of epidermal projections. One wound at 18 weeks postwounding had histological features typical of a keloid. 相似文献
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M. J. LEES DVM MVSC C. V. BOWEN FRCSC P. B. FRETZ VMD DiplomateACVS D. H. LEACH PhD 《Veterinary surgery : VS》1989,18(4):292-299
Eighteen deep circumflex iliac flaps were elevated in healthy adult horses. Four flaps survived well when elevated as islands, but five orthotopic flaps and nine heterotopic flaps transferred to the tarsus and face failed. Technical reasons could explain the failure of the orthotopic flaps, but not the heterotopic flaps. Failure of the heterotopic flaps was apparently caused by the no-reflow phenomenon. 相似文献