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Eight heifers, aged 16–17 months and showing normal oestrous cycles, were immunized against a recombinant porcine inhibin α subunit immunogen, together with another 10 heifers of the same age as controls and treated with placebo immunogen. Primary (1 mg immunogen) and two booster (0.5 mg immunogen each) immunizations were administered at 28‐day intervals. Ten days after the second booster immunization, both groups of heifers underwent a superovulation treatment. Each animal was given an intravaginal progesterone releasing sponge, which was withdrawn 7 days following an i.m. injection of 0.5 mg cloprostenol. Heifers were treated with FSH for 4 days and artificially inseminated after oestrus occurred. The embryos were flushed and evaluated 7 days after insemination. Immunization significantly (p < 0.01) increased blood antibody titres against recombinant porcine inhibin α subunit, from pre‐immunizaion and control values of approximately 0.06 of ELISA 450 nm reading to 0.6 to 0.7 after two or three immunizations. The immunized heifers produced on average 15.8 ± 2.8 embryos, significantly (p < 0.05) higher than the yield of 8.3 ± 1.5 in the controls. The number of transferable embryos were non‐significantly higher in immunized than in control heifers (9.6 ± 3.1 vs 5.8 ± 1.6, p > 0.05). The peak plasma oestradiol concentrations were significantly higher in immunized than in control heifers, both immediately after FSH treatment and 20 days thereafter. Plasma P4 concentrations after superovulation were in the range of 20 ng / ml in the immunized heifers, significantly (p < 0.05) higher than the values approximately 15 ng / ml in control heifers. These results indicated that prior immunization against inhibin α subunit stimulated production of antibodies against inhibin, which enhanced follicular developmental response to superovulation and lead to higher yield of total and transferable embryos. Therefore immunization combined with the conventional superovulatory gonadotrophin treatment, can be a simple and efficient method to produce low cost bovine embryos. 相似文献
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S. Q. Lafi A. Q. Talafha N. Giadinis E. Kalaitzakis K. Pourliotis N. Panousis 《Tropical animal health and production》2009,41(8):1755-1764
A prospective cohort study was conducted using 32 randomly selected Awassi sheep flocks to identify factors hypothesized to be associated with the occurrence of pregnancy, twinning and fetal loss between August 2005 and May 2006 in the region of Al-Safawi (northeast of Jordan). Vitamins A and E and selenium concentrations were determined on 448 blood samples. Using the forward selection procedure of the logistic regression module, models with statistically significant risk factors (P?<?0.05) were constructed for three outcomes; pregnancy, twinning and fetal loss. Serum vitamin A concentration levels were associated with pregnancy (OR?=?2.26, 2.48), twinning (OR?=?6.49, 17.74) and fetal loss (OR?=?0.13, 0.19). Primiparous ewes were 48% less likely to become pregnant than fourth or higher parity ewes. The likelihood of twinning increased significantly in ewes up to the third parity. Ewes that were fed 700-900 g barley, 250-300 g wheat-bran per head per day and grazed on vegetables residues were 4.15 times more likely to have twins than ewes that were fed 600 g barley and 200 g wheat-bran per head per day. Fetal loss in first and second parity ewes was about 3 times more likely than that in third or higher-parity ewes. Ewes pregnant with twins were about 14 times more likely to have fetal loss than ewes carrying single fetus. Pregnant ewes of the stationary flocks were 37% less likely to have fetal loss than ewes of the semi-nomadic flocks. These results demonstrate that stationary Awassi sheep flocks had higher pregnancy and twinning rates and less pregnancy loss. Supplementation of vitamin A, providing sufficient quantity of dry feed and increasing ram: ewe ratio for primiparous ewes of semi-nomadic flocks is essential to improve Awassi sheep reproductive performance. 相似文献
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Giadinis ND Papadopoulos E Panousis N Papazahariadou M Lafi SQ Karatzias H 《Journal of veterinary pharmacology and therapeutics》2007,30(6):578-582
This study aimed to investigate the effects of halofuginone lactate (100 mug/kg BW) on treatment and prevention of cryptosporidiosis in lambs. It consisted of three field trials. The first trial was designed to assess the efficacy of halofuginone in treating lamb diarrhoea caused by cryptosporidiosis, and in preventing the disease, using two schemes; halofuginone given for seven and for three consecutive days respectively. Halofuginone was effective in the treatment of diarrhoea caused by cryptosporidiosis (P < 0.01). In addition, halofuginone administered as a 7-day treatment was significantly (P < 0.05) more effective than a 3-day treatment in preventing diarrhoea in the infected flocks. The second trial was designed to evaluate the preventive effect of halofuginone, which was administered for 7 days in lambs infected with cryptosporidiosis, on diarrhoea incidence, oocyst shedding and body weight gain. Halofuginone significantly (P < 0.01) reduced the diarrhoea incidence, the time of oocyst shedding and the mean intensity of shedding, but did not affect body weight gain. The third trial was designed to examine the ability of halofuginone to reduce the death rate in flocks with cryptosporidiosis. Halofuginone treatment was effective in preventing and in reducing the death rate of cryptosporidiosis in these flocks. 相似文献
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Soha Al-Amoudi Magbubah Essack Marta F. Sim?es Salim Bougouffa Irina Soloviev John A. C. Archer Feras F. Lafi Vladimir B. Bajic 《Marine drugs》2016,14(9)
Microorganisms that inhabit unchartered unique soil such as in the highly saline and hot Red Sea lagoons on the Saudi Arabian coastline, represent untapped sources of potentially new bioactive compounds. In this study, a culture-dependent approach was applied to three types of sediments: mangrove mud (MN), microbial mat (MM), and barren soil (BS), collected from Rabigh harbor lagoon (RHL) and Al-Kharrar lagoon (AKL). The isolated bacteria were evaluated for their potential to produce bioactive compounds. The phylogenetic characterization of 251 bacterial isolates based on the 16S rRNA gene sequencing, supported their assignment to five different phyla: Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, and Planctomycetes. Fifteen putative novel species were identified based on a 16S rRNA gene sequence similarity to other strain sequences in the NCBI database, being ≤98%. We demonstrate that 49 of the 251 isolates exhibit the potential to produce antimicrobial compounds. Additionally, at least one type of biosynthetic gene sequence, responsible for the synthesis of secondary metabolites, was recovered from 25 of the 49 isolates. Moreover, 10 of the isolates had a growth inhibition effect towards Staphylococcus aureus, Salmonella typhimurium and Pseudomonas syringae. We report the previously unknown antimicrobial activity of B. borstelensis, P. dendritiformis and M. salipaludis against all three indicator pathogens. Our study demonstrates the evidence of diverse cultured microbes associated with the Red Sea harbor/lagoon environments and their potential to produce antimicrobial compounds. 相似文献
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Abu-Dalbouh MA Ababneh MM Giadinis ND Lafi SQ 《Tropical animal health and production》2012,44(1):49-54
Two hundred and fifty-five biological samples (106 aborted foetal tissue samples and 149 blood samples from aborted sheep
and goats) were collected from 188 animals during the lambing season from September 2009 to April 2010 from the Mafraq region
of Jordan. The sampled animals belonged to 93 goat and sheep flocks that had cases of abortion. A total of 169 (66.3%) biological
samples were collected from sheep and 86 (33.7%) from goats. Seventy-six (29.8%) biological samples (45 blood and 31 tissue
samples) were positive for Toxoplasma gondii by PCR assay. The positive samples were obtained from 43 sheep and 23 goats. The overall toxoplasma-specific prevalence rate
was 35.1% (66/188). Forty flocks (43%) had at least one T. gondii PCR-positive animal. The risk factors related to flock health status and farm management that are hypothesized to be associated
with T. gondii PCR positivity were also assessed using multiple logistic regressions. The presence of cats (OR = 4.74), a large flock size
(OR = 2.76) and the method of disposing the aborted foetuses (OR = 3.77) were all statistically significant (P < 0.05) risk factors that were positively associated with toxoplasma positivity in goat and sheep flocks. 相似文献
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近些年来 ,葫芦岛地区冬麦生产在逐年扩大 ,为了提高冬麦产量和以冬麦为前茬调整和优化种植业结构 ,以增加农民收入 ,我们围绕冬麦生产开展了一些试验与示范。现将试验结果初报如下 ,供参考。1 冬麦品种筛选1 .1 抗寒性鉴定通过在建昌县和尚房子乡、兴城市大寨乡和连山区金星镇试验田上鉴定结果表明 ,京 41 1越冬抗寒性居首 ,其越冬死亡率在 0～ 3.0 %。越冬死亡率最高的品种为 950 2 1 ,达 76.3%(引自金州区种子公司 )。京冬 8号越冬死亡率 0～ 1 8.4%。据调查 ,越冬死亡率在 1 5.0 %以内 ,返青后每667ｍ2 存活苗数达 1 5万株左右 ,仍然… 相似文献