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A horse presented with a discharging wound over the proximal aspect of the right metatarsal region. Examination, radiography and ultrasonography diagnosed a septic process within the accessory ligament of the deep digital flexor tendon (ALDDFT). Due to previous unsuccessful treatment with systemic antibiotics as well as financial restrictions, an ALDDFT desmectomy was performed with the patient under standing sedation and local analgesia. Histopathology confirmed the septic process within the affected structure. The patient made a rapid recovery and was discharged from the hospital 7 days post operatively and 2 years later was still in full work. This report describes the first case of infective hindlimb ALDDFT and the first desmectomy of this structure performed under standing sedation. 相似文献
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Budiño B Lamas J Pata MP Arranz JA Sanmartín ML Leiro J 《Veterinary parasitology》2011,175(3-4):260-272
Research on intraspecific variation in ciliates is scarce, and in scuticociliate parasite of fish, virtually nonexistent. In this study, seven isolates obtained from turbots affected by scuticociliatosis in different parts of the Iberian Peninsula (northwest Spain and southwest Portugal) were morphologically and genetically characterized to investigate the intraspecific divergence in these amphizoic ciliates. The isolates were stained with ammoniacal silver carbonate and examined in an optical microscope; all were found to have the typical morphological characteristics described for Philasterides dicentrarchi (syn. Miamiensis avidus). Sixteen biometric characteristics of the seven isolates were used in a canonical discrimination analysis (CDA) to select a subset of those that best identified each isolate. Discriminant analysis indicated that the OPK3 width, length of the PM2, length of the buccal field, the body width, L:W ratio, the body length, the OPK1 width and the distance between OPK2 and OPK3 were the most important morphological variables for discriminating the isolates. The first three canonical functions accounted for 86% of the total variance. The scatter plots of the first two canonical variables grouped and separated the P. dicentrarchi isolates into five clusters. Flow cytometry analysis of isolates also indicated intraspecific polymorphisms among P. dicentrarchi isolates. Nuclear markers (a 349-bp and a 390-bp fragment of 18S rRNA and β-tubulin genes) and a 398-bp of the mitochondrial cytocrome oxidase subunit I (Cox1) gene were then used to investigate the intraspecific genetic variation in P. dicentrarchi. Haplotype analysis and neighbour-joining phylogenies of nucleotide sequences of seven isolates revealed a high degree of intraspecific genetic variation among the isolates. Analysis of Cox1 and β-tubulin genes revealed six haplotypes (and clusters) in both cases; however, analysis of the 18S rRNA gene revealed only two haplotypes. The results show clear intraspecific variation at morphological and genetic levels in the scuticociliate P. dicentrarchi, and verify the suitability of mitochondrial (Cox1) and nuclear (β-tubulin) genes for detecting intraspecific genetic variation within populations of scuticociliates that infect cultured turbot. The existence of this intraspecific variation must be taken into account in the design of an effective vaccine to control scuticociliatosis. 相似文献
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Lamas LP Edmonds J Hodge W Zamora-Vera L Burford J Coomer R Munroe G 《Equine veterinary journal》2012,44(4):399-403
Reasons for performing this study: Intra‐articular ethanol has been described to promote distal tarsal joint ankylosis. Its use and results in clinical cases affected by osteoarthritis (OA) have not been reported. Objectives: To describe and evaluate the results of treatment of distal tarsal joint OA by facilitated ankylosis stimulated by intra‐articular ethanol injection. Methods: Twenty‐four horses met the inclusion criteria of tarsometatarsal and centrodistal joint OA diagnosed by a positive response to intra‐articular analgesia, radiographic evaluation and recurrence of lameness ≤4 months after intra‐articular medication with a corticosteroid. Horses were sedated and, following a radiographic contrast study of the tarsometatarsal joint, medication with 2–4 ml of either 100% pure ethanol (G100) or a 70% ethanol (G70) solution was applied. Horses were classified as improved based on a 50% reduction from initial lameness grade combined with an increase in exercise level. Results: Of the 24 horses included in this study, 20 had the treatment performed bilaterally and 4 unilaterally. All horses were available for initial follow‐up examination and 21 for a second one 6–9 months after treatment. This represented a total of 44 treated limbs and 35 available for long‐term follow‐up. Of these, 21/35 (60%) were considered improved, which corresponds to 11/21 horses (52%). Of 21 horses, 4 (19%) deteriorated and 2 of these developed significant complications related to treatment. Conclusions: Distal tarsal joint ankylosis with ethanol should be considered a safe and economic treatment in cases of distal tarsal joint OA that fail to show long‐term improvement with intra‐articular corticosteroid treatment. Potential relevance: Ethanol should be considered in the treatment of certain cases of distal tarsal joint OA. The importance of performing an adequate radiographic contrast study of the tarsometatarsal joint prior to treatment is highlighted. 相似文献
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M Asakawa JW Ludders B Badgley HN Erb LP Posner RD Gleed 《Veterinary anaesthesia and analgesia》2005,32(4):12-13
Adenosine is a potent analgesic in people and reduces the MAC of halothane in dogs. The purpose of this study was to determine whether adenosine reduces the MAC of isoflurane in dogs. Seven beagles (four males and three females) were anesthetized and randomly assigned to receive adenosine (0.3 mg kg–1 minute–1; 6 mL kg–1 hour–1, IV) or saline (0.9%, 6 mL kg–1 hour–1IV). After an interval of ≥7 days, each dog was reanesthetized and treated with the alternate infusion. Anesthesia was induced and maintained with isoflurane in oxygen. Dogs were intubated and instrumented for measurement of mean systemic arterial blood pressure and airway concentration of isoflurane and end‐tidal partial pressure of carbon dioxide. The MAC for isoflurane was determined using the tail‐clamp technique. Baseline MAC values were 1.25 (1.15, 1.35)% [median (minimum, maximum)] and 1.25 (1.05, 1.45)% before the saline and adenosine treatments, respectively. After 2 hours of infusion with saline or adenosine, MAC values were not different (p = 0.156) and were 1.25 (0.95, 1.35)% and 1.05 (1.00, 1.25)%, respectively. Two hours following the end of each infusion, the MAC values for saline and adenosine treatment groups differed significantly (p = 0.015), but by no more than the normal variation inherent in this study, and were 1.15 (1.15, 1.35)% and 1.05 (1.05, 1.25)%, respectively. Mean arterial blood pressure was 93 (74, 123) mm Hg for saline treated dogs and 67 (52, 72) mm Hg (p = 0.008) during adenosine infusion. End‐tidal carbon dioxide was not different between the two treatment groups. We conclude that adenosine administered at 0.3 mg kg–1 minute–1had no effect on isoflurane MAC in dogs, but decreased mean arterial blood pressure. 相似文献
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Aftertaste is a taste perceiption after a food or beverage is either swallowed or spat out.The primary taste processing area located in the insula was considered to be involved in aftertaste perception.The changes in chemical compositions lead to the changes in aftertaste of tea.Tea fermentation,in which tea catechins are transformed into thearubigins,lead to a strong,distinctive flavor but a plain aftertaste.Theanine has a sweet taste,with little or no aftertaste.The bitter aftertaste of transglucosylated steviosides was reduced when they were converted through transglucosylation of stevioside by α-amylase.Characteristics of aftertaste include quality,intensity,and duration.Foods that have lingering aftertastes typically have long sensation durations.A surface plasmon resonance （SPR） system immobilized β-cyclodextrin indicated larger responses for the gallate-type catechins in comparison to the non-gallate-type catechins.The β-cyclodextrin/SPR system can sense the bitter-astringent taste intensity of the green tea.The SPR system detected the stability of the complex between the gallate-type catechins and β-cyclodextrin,which can be interpreted as the aftertaste produced in humans by the gallate-type catechins. 相似文献
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以发生于本市的一起兔病毒性出血症与大肠杆菌病混合感染的病例为题材，从发病情况、临床症状、病变、实验室检查、防治等几个方面作了详尽的阐述，为临床上治疗该病提供参考依据。 相似文献
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Objective— To report repair of a longitudinal scapular fracture in a horse.
Study Design— Case report.
Animals— A 2-year-old Paint Horse colt.
Methods— A longitudinal scapular fracture was surgically repaired using four 4.5 mm dynamic compression plates.
Results— An acute longitudinal scapular fracture repaired surgically returned the horse to soundness within 6 months.
Conclusions— Internal fixation of longitudinal scapular fracture is possible with multiple 3–5 hole dynamic compression plates.
Clinical Relevance— Longitudinal fractures of the scapula should be considered when there is lateral instability of the shoulder after trauma. A displaced fracture can be adequately stabilized by internal fixation with a reasonable prognosis for soundness. 相似文献
Study Design— Case report.
Animals— A 2-year-old Paint Horse colt.
Methods— A longitudinal scapular fracture was surgically repaired using four 4.5 mm dynamic compression plates.
Results— An acute longitudinal scapular fracture repaired surgically returned the horse to soundness within 6 months.
Conclusions— Internal fixation of longitudinal scapular fracture is possible with multiple 3–5 hole dynamic compression plates.
Clinical Relevance— Longitudinal fractures of the scapula should be considered when there is lateral instability of the shoulder after trauma. A displaced fracture can be adequately stabilized by internal fixation with a reasonable prognosis for soundness. 相似文献
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The scuticociliatosis produced by the endoparasite Philasterides dicentarchi is a severe parasitic infection of farmed turbot (Scophthalmus maximus) characterized by several histopathological effects including extensive inflammation. Indomethacin is a nonsteroidal anti-inflammatory drug that specifically inhibits synthesis of the proinflammatory mediator prostaglandins. The effect of indomethacin on the in vitro growth of P. dicentrarchi was investigated. In vitro growth of the scuticociliate was significantly inhibited by treatment with 100 microM indomethacin for 48 h. Higher concentrations of indomethacin (mM levels) did not affect the gelatinolytic activity of the cysteine proteinases of P. dicentrarchi. In vitro treatment with 25, 50 or 100 microM indomethacin for 3 days did not significantly affect the enzymatic activity of cysteine proteinases, as assayed with p-nitroanilide as substrate. Immunoblot analysis with anti-cysteine proteinase antibodies revealed an increase in proteinase expression (molecular weights of 80, 32 and 40-45 kDa) in parasite lysates originating from in vitro cultures incubated with 25 microM indomethacin for 72 h. Degradation of genomic DNA of the ciliates was observed in cultures incubated with 100 microM indomethacin for 1, 3 and 7 days. The results suggest that indomethacin is capable of inhibiting in vitro growth of the scuticociliate P. dicentrarchi by a mechanism related to the induction of programmed cell death, without affecting the enzymatic activation of parasite proteinases, which demonstrates the potential therapeutic use of this drug in the control of turbot scuticociliatosis. 相似文献