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Haematological and biochemical investigations were performed on 14 koalas with uncomplicated cystitis, 8 with complicated cystitis, 8 with conjunctivitis, 8 with lymphosarcoma, and 14 with miscellaneous diseases. Changes were limited and inconsistent in individual koalas with uncomplicated cystitis and conjunctivitis. In contrast, individual koalas with complicated cystitis were more likely to have anaemia, leukocytosis due to neutrophilia, hypoproteinaemia due to hypoalbuminaemia, and azotaemia due to elevated urea concentration. Although these changes were non-specific they did allow assessment of prognosis for survival and response to treatment. Koalas with lymphosarcoma were invariably anaemic, leukaemic, azotaemic and hypoalbuminaemic. Elevated enzymes (aspartate transaminase [AST]. lactate dehydrogenase [LD] and gamma glutamyl transferase [GGT]) were more common in koalas with lymphosarcoma. Koalas affected by miscellaneous conditions showed variable changes but once again anaemia, leukocytosis, azotaemia, elevated AST and LD, and hypoalbuminaemia were not uncommon. On the basis of these findings a minimal profile is suggested for the investigation of sick koalas and would include haematocrit, total and differential leukocyte counts, urea, total protein and albumin concentrations and AST, GGT and LD activities. 相似文献
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Kirtz G Meli M Leidinger E Ludwig P Thum D Czettel B Kölbl S Lutz H 《The Journal of small animal practice》2005,46(6):300-303
A diagnosis of Anaplasma phagocytophilum infection was confirmed in a two-year-old male golden retriever displaying few clinical and haematological abnormalities. This was achieved by demonstrating ehrlichial organisms in circulating neutrophils, by indirect immunofluorescence assay using A phagocytophilum as an antigen, and by detecting DNA specific for the 16S rRNA gene of granulocytic Anaplasma by PCR. After treatment with doxycycline for 10 days the dog showed improvement and the laboratory values returned to normal. 相似文献
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Ernst F. Leidinger Judith Leidinger Julia Figl Bettina Rumpler Ilse Schwendenwein 《Acta veterinaria Scandinavica》2015,57(1)
Background
Despite the increasing popularity of Icelandic horses, published reference intervals (RIs) in this breed are rare. Due to their isolation and their small gene pool, alterations in some variables are likely and some possible breed-specific peculiarities have been described. The purpose of the present study was the establishment of comprehensive RIs in Icelandic horses according to recently published guidelines.In a prospective observational study, blood samples were collected from the jugular vein of 142 Icelandic horses into EDTA and serum tubes. Reference intervals were established for haematologic and biochemical analytes on the Advia 2120i™ and the Dimension ExL™ by established methods. RIs were defined as central 95 % intervals bounded by the 2.5th and 97.5th percentiles with their 90 % confidence intervals, calculated according to recently published ASVCP guidelines. An inhouse-developed quality control system using observed total allowable error was used for the surveillance of the internal quality control preceding the measurements.Results
The RIs were as follows: haematocrit: 0.29–0.39, RBC: 5.79–8.63 T/l, haemoglobin: 102.0–142.3 g/l, MCV: 42–51 fl, platelets: 146–263 G/l, WBC: 4.13–8.57 G/l, segs: 1.98–4.73 G/l, lymphocytes: 1.25–3.49 G/l, monocytes: 0.06–0.31 G/l, eosinophils: 0.04–0.50 G/l, glucose: 4.0–5.7 mmol/l, urea: 3.2–6.4 mmol/l, creatinine: 79.6–141.4 μmol/l, total protein: 54.4–72.9 g/l, albumin: 27.7–36.8 g/l, total bilirubin: 8.1–21.1 μmol/l, triglycerides: 0.03–0.44 mmol/l, cholesterol: 1.75–2.90 mmol/l, ALP: 1.35–3.55 μkat/l, AST: 4.52–8.80 μkat/l, GLDH: 0.0–0.18 μkat/l, GGT: 0.11–0.39 μkat/l, CK: 2.53–6.52 μkat/l, LDH: 3.32–7.95 μkat/l, iron: 16.4–39.9 μmol/l, calcium: 2.69–3.19 mmol/l, phosphate: 0.5–1.3 mmol/l, magnesium: 0.6–0.9 mmol/l, sodium: 134–141 mmol/l, potassium: 3.6–4.7 mmol/l, chloride: 100–105 mmol/l.Conclusions
Reference intervals of several haematologic and biochemical analytes differed from the transferred historical reference intervals applied to equine samples in the authors’ laboratory. These might be of clinical importance in some analytes such as creatine kinase.Electronic supplementary material
The online version of this article (doi:10.1186/s13028-015-0120-4) contains supplementary material, which is available to authorized users. 相似文献5.
E. Leidinger E. Kramberger-Kaplan Manfred Gemeiner 《Comparative immunology, microbiology and infectious diseases》1993,16(4):289-298
Feline splenocytes were fused to the murine myeloma lines NSO or Ag8. Autologous serum and taurine were used as media supplements for the cat × mouse heterohybridomas. The best results were obtained by the use of NSO as fusion line with taurine-supported media. 相似文献
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E. Furman E. Leidinger E.H. Hooijberg N. Bauer G. Beddies A. Moritz 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(5):1391-1397
Background
Frequency and classification of anemia in terms of regeneration status and erythrocyte indices are not well described in cats.Objective
To determine frequency and regenerative status of anemia in samples from adult cats, to assess the sensitivity and specificity of macrocytosis and hypochromasia for detecting regenerative anemia (RA), and to evaluate the association of anemia with increased serum creatinine concentration (SC).Study Population
Laboratory records from 30,503 blood samples from cats (2003–2011).Methods
Clinicopathologic data reviewed retrospectively. Anemia defined as hematocrit (Ht) ≤27%, red blood cell count (RBC) ≤5.5 × 106/μL and hemoglobin (Hb) ≤9.0 g/dL. RA defined by manual absolute reticulocyte count >50 × 103/μL. Macrocytosis was defined as mean corpuscular volume (MCV) >55 fL and hypochromasia as mean corpuscular hemoglobin concentration (MCHC) <31 g/dL. Cutoff for increased serum creatinine concentration was 1.6 mg/dL.Results
Overall, 1,098 of 30,503 blood samples (3.6%) from cats fulfilled criteria for anemia, 633 of 1,098 (57.7%) classified as nonregenerative (NRA) and 465 of 1,098 (42.3%) as regenerative. RBC, Ht, and Hb were significantly lower in the RA compared to NRA group (P < .05). Sensitivity and specificity of the combined high MCV and low MCHC to detect samples with RA were 19.5 and 90.7%. SC was increased in 572 of the 1,098 anemic samples (52.1%) and in 11,121 of 29,405 of nonanemic samples (37.8%).Conclusions and Clinical Importance
Majority of anemic samples were classified as NRA. Anemia was more severe in cats with RA. Erythrocyte indices were not sensitive indicators of RA. 相似文献7.
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CA Souza NM Ocarino JF Silva JN Boeloni EF Nascimento IJ Silva RD Castro LP Moreira FRCL Almeida H Chiarini‐Garcia R Serakides 《Reproduction in domestic animals》2011,46(1):e7-e16
The aim of this study was to evaluate the effects of thyroxine administration on morphometric parameters, expression of vascular endothelial growth factor (VEGF) and vascularization in the uterus and placenta and reproductive parameters in gilts at 70 days of gestation. At 150 days of age, i.e., before first heat, 20 gilts were randomly divided into two experimental groups: treated (n = 10) and control (n = 10). The treated group received a daily dose of 400 μg of l ‐thyroxine (T4) in their diet until slaughter and the control group received only typical meals. Before artificial insemination, blood was collected to determine plasma total T4. The gilts were inseminated in the second oestrus and slaughtered at 70 days of gestation. The foetal thyroid follicular epithelium height, number, size and weight of foetuses; foetal myogenesis, corpora lutea number, embryonic mortality rate, uterine weight, placental weight and placental fluid volume were measured. Histomorphometric and immunohistochemical analysis of uterus and placenta were determined. The averages of all variables were compared by the Student’s t‐test. The gilts treated with thyroxine showed significant increase of plasma total T4. At 70 days of gestation, the heights of the trophoblastic epithelium, endometrial epithelium and endometrial gland epithelium were significantly higher in the group treated with T4. The expression of cytoplasmatic and nuclear VEGF in trophoblastic cells and the number of blood vessels per field in endometrial stroma were significantly higher in the gilts treated with T4. No other significant differences between groups were obtained with respect to other parameters (p > 0.05). We conclude that oral administration of T4 up to 70 days of pregnancy in gilts affects the morphometric parameters, the expression of placental VEGF and the uterine vascularization but does not affect reproductive parameters in gilts during early gestation. 相似文献
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MR N'Diaye SS Sun SP Fanua KJ Loseth EF Shaw Wilgis BG Crabo 《Reproduction in domestic animals》2002,37(5):305-309
Supplementation of exogenous growth hormone (GH) during prepuberty advances onset of spermatogenesis in boars, but the mechanism of action is unknown. The present study is an investigation of the presence and characteristics of testicular growth hormone receptors (GHR). A total of 36 boars were castrated, three boars every 10 days, between the ages of 10 and 120 days. Testicular membrane preparations of 10, 20, 30, 50, 70, 100 and 120‐day‐old boars were used to determine 125I‐bGH binding and Scatchard analysis. Liver from a 60‐kg barrow was used for comparison. Specific 125I‐bGH binding to testicular membrane preparations occurred in all age groups with the exception of 20‐day‐old boars at levels of 30–40% of liver binding. At 30 days of age the unlabelled bGH at 1.1 ng/tube achieved half maximal inhibition (ID50). Results of Scatchard analysis indicated a single class of binding sites. Binding affinity was 2.89 × 109m with a binding capacity of 12 fmole/mg membrane protein. The results from this study suggest that GH may act directly on the cells of the prepubertal boar testis. 相似文献
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JF Silva NM Ocarino ALS Vieira EF Nascimento R Serakides 《Reproduction in domestic animals》2013,48(4):691-698
Although thyroid dysfunction occurs frequently in humans and some animal species, the mechanisms by which hypo‐ and hyperthyroidism affect the corpus luteum have not been thoroughly elucidated. This study evaluated the levels of proliferative activity, angiogenesis, apoptosis and expression of cyclooxygenase‐2 in the corpus luteum of female rats with thyroid dysfunction. These processes may be important in understanding the reproductive changes caused by thyroid dysfunction. A total of 18 adult female rats were divided into three groups (control, hypothyroid and hyperthyroid) with six animals per group. Three months after treatment to induce thyroid dysfunction, the rats were euthanized in the dioestrus phase. The ovaries were collected and immunohistochemically analysed for expression of the cell proliferation marker CDC‐47, vascular endothelial growth factor (VEGF), VEGF receptor Flk‐1 and cyclooxygenase‐2 (COX‐2). Apoptosis was evaluated using the TUNEL assay. Hypothyroidism reduced the intensity and area of COX‐2 expression in the corpus luteum (p < 0.05), while hyperthyroidism did not alter COX‐2 expression in the dioestrus phase. Hypothyroidism significantly reduced the expression of CDC‐47 in endothelial cells and pericytes in the corpus luteum, whereas hyperthyroidism did not induce a detectable change in CDC‐47 expression (p > 0.05). Hypothyroidism reduced the level of apoptosis in luteal cells (p < 0.05) and increased VEGF expression in the corpus luteum. In contrast, hyperthyroidism increased the level of apoptosis in the corpus luteum (p < 0.05). In conclusion, thyroid dysfunction differentially affects the levels of proliferative activity, angiogenesis and apoptosis and COX‐2 expression in the corpus luteum of female rats. 相似文献