Production and characterization of monoclonal antibodies to budgerigar fledgling disease virus major capsid protein VP.     

A Fattaey  L Lenz  R A Consigli 《Avian diseases》1992,36(3):543-553

Eleven hybridoma cell lines producing monoclonal antibodies (MAbs) against intact budgerigar fledgling disease (BFD) virions were produced and characterized. These antibodies were selected for their ability to react with BFD virions in an enzyme-linked immunosorbent assay. Each of these antibodies was reactive in the immunofluorescent detection of BFD virus-infected cells. These antibodies immunoprecipitated intact virions and specifically recognized the major capsid protein, VP1, of the dissociated virion. The MAbs were found to preferentially recognize native BFD virus capsid protein when compared with denatured virus protein. These MAbs were capable of detecting BFD virus protein in chicken embryonated cell-culture lysates by dot-blot analysis.  相似文献   

Evaluation of the diagnosis of subclinical endometritis in dairy cattle using ultrasound     

Lenz M  Drillich M  Heuwieser W 《Berliner und Münchener tier?rztliche Wochenschrift》2007,120(5-6):237-244

The aim of this study was to determine signs of subclinical endometritis found by ultrasound that are associated with reduced fertility in dairy cows.The maximum diameter of the uterine lumen was determined by ultrasound in 324 cows without clinical signs of endometritis after evaluation of the genital tract 21 to 27 days postpartum. Cows were classified into healthy or with subclinical endometritis by three threshold values for the maximum uterine lumen diameter of 0.2 cm, 0.5 cm or 0.8 cm. Examinations by rectal palpation and ultrasound as well as classifications were repeated 14 days later. In addition, ovaries were scanned by ultrasound to determine the stage of the estrous cycle. In a subgroup of 103 cows the echotexture of the uterus and its contents was evaluated. In these cows the diagnosis of subclinical endometritis was performed by a scoring system. The diameter of the uterine lumen was significantly affected by stage of the estrous cycle at the time of examination. However, no effects were found for the stage of the cycle at the time of examination on subsequent reproductive performance. A uterine lumen with a maximum diameter of more than 0.2 cm showed a significant negative association with conception rate and proportion of cows pregnant. Classification based on higher threshold values did not reveal an association with reproductive performance. Echogenic content in the uterus also decreased reproductive performance. A classification based on the echotexture of the uterus and its contents revealed significant differences between healthy cows and cows with subclinical endometritis regarding the proportion of cows inseminated and pregnant.The results of this study showed that the diagnostic of bovine endometritis should be broadend by ultrasonography. The definition of subclinical endometritis diagnosed by means of ultrasonography has to be evaluated in further studies.  相似文献   

Evaluation of mechlorethamine,vinblastine, procarbazine,and prednisone for the treatment of resistant multicentric canine lymphoma     

Kelley Zimmerman  Koranda A. Walsh  Jonathan T. Ferrari  Nicholas S. Keuler  Matthew J. Atherton  Jennifer A. Lenz 《Veterinary and comparative oncology》2023,21(3):503-508

Multi-agent chemotherapy successfully induces remission in most naïve, high-grade canine lymphoma patients; however, disease recurrence is common. MOPP (mechlorethamine, vincristine, procarbazine, and prednisone) is an effective rescue protocol used to re-induce remission, but is associated with gastrointestinal toxicity and can be a less desirable option for patients that previously failed vincristine-containing protocols. Therefore, alternative members of the vinca alkaloid family, such as vinblastine, could be potentially advantageous as substitutes for vincristine to reduce gastrointestinal toxicity and chemoresistance. The objective of this study was to report the clinical outcomes and toxicity of 36 dogs with relapsed or refractory multicentric lymphoma treated with a modified MOPP protocol whereby vincristine was replaced with vinblastine (MVPP). The overall response rate to MVPP was 25% with a median progression free survival of 15 days and a median overall survival of 45 days. MVPP at the prescribed doses resulted in modest and transient clinical benefit, but was well tolerated with no treatment delays or hospitalizations secondary to side effects. Given the minimal toxicity, dose intensification could be considered to improve clinical responses.  相似文献   

Coccygeal muscle injury in English Pointers (limber tail)     

Steiss J  Braund K  Wright J  Lenz S  Hudson J  Brawner W  Hathcock J  Purohit R  Bell L  Horne R 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1999,13(6):540-548

A condition colloquially referred to as "limber tail" and "cold tail" is familiar to people working with hunting dogs, primarily Pointers and Labrador Retrievers. The typical case consists of an adult dog that suddenly develops a flaccid tail. The tail either hangs down from the tail base or is held out horizontally for several inches from the tail base and then hangs straight down or at some degree below horizontal. Initially, the hair on the dorsal aspect of the proximal tail may be raised and dogs may resent palpation of the area 3-4 inches (8-10 cm) from the tail base. Most dogs recover spontaneously within a few days to weeks. Anecdotal reports suggest that anti-inflammatory drugs administered within 24 hours after onset hasten recovery. Less than one half of affected dogs experience a recurrence. Affected Pointers almost always have a history of prolonged cage transport, a hard workout the previous day, or exposure to cold or wet weather Most owners and trainers familiar with the condition do not seek veterinary assistance. In cases where people are not familiar with this disease, other conditions such as a fracture, spinal cord disease, impacted anal glands, or prostatic disease have been incorrectly diagnosed. We examined 4 affected Pointers and found evidence of coccygeal muscle damage, which included mild elevation of creatine kinase early after onset of clinical signs, needle electromyographic examination showing abnormal spontaneous discharges restricted to the coccygeal muscles several days after onset, and histopathologic evidence of muscle fiber damage. Specific muscle groups, namely the laterally positioned intertransversarius ventralis caudalis muscles, were affected most severely. Abnormal findings on thermography and scintigraphy further supported the diagnosis.  相似文献   

Effects of road transportation or droving on the weight and metabolism of young bulls   总被引：1，自引：0，他引：1  

Marcella Cândia D’Oliveira  Maria Inês Lenz Souza  Ruy Alberto Caetano Corrêa Filho  Maria da Graça Morais  Camila Celeste Brandão Ferreira Ítavo  Gumercindo Loriano Franco 《Tropical animal health and production》2014,46(8):1447-1453

This study evaluated the effects of the mode of transportation on weight and metabolism of young bulls, which are generally transported by two systems: droving and truck. This experiment consisted of two phases: transportation and refeeding and involved transportation by droving and truck. Fifty young Nellore bulls were separated in equal numbers in both groups, droving group and truck group. In the transportation phase, animals were moved, in a 640-km journey. The refeeding phase consisted of 84 days and commenced after the animals arrived at the fattening farm. The effect of the transportation system on animals’ weight, metabolic hormones, and serum biochemistry was assessed after 28, 56, and 84 days. During the transportation phase, animals in the droving group lost 10.11 kg and animals in the truck group gained 13.1 kg. The truck group showed 20.5 kg of weight gain at the end of the refeeding phase. The highest triiodothyronine (T₃) and thyroxine (T₄) concentrations were observed on days 0 and 60 in the transportation phase in both droving and truck groups. The serum cortisol concentration was higher in the droving group than in the truck group on day 56 of the refeeding phase. The IGF-I concentrations were higher during the refeeding phase in the droving group than in the truck group. Our results showed that transportation by droving leads to increased weight loss and reduced further weight gain compared to transportation by truck and that serum concentrations of T₄, cortisol, and insulin-like growth factor (IGF-I) hormones are altered by the transportation system.  相似文献   

Herrn Prof. Dr. Dr. h. c. K. Schmalfuß zum 65. Geburtstag     

K. Lenz 《Archives of Agronomy and Soil Science》2013,59(10):908-911

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Sequential establishment of stripe patterns in an expanding cell population     

Liu C  Fu X  Liu L  Ren X  Chau CK  Li S  Xiang L  Zeng H  Chen G  Tang LH  Lenz P  Cui X  Huang W  Hwa T  Huang JD 《Science (New York, N.Y.)》2011,334(6053):238-241

Periodic stripe patterns are ubiquitous in living organisms, yet the underlying developmental processes are complex and difficult to disentangle. We describe a synthetic genetic circuit that couples cell density and motility. This system enabled programmed Escherichia coli cells to form periodic stripes of high and low cell densities sequentially and autonomously. Theoretical and experimental analyses reveal that the spatial structure arises from a recurrent aggregation process at the front of the continuously expanding cell population. The number of stripes formed could be tuned by modulating the basal expression of a single gene. The results establish motility control as a simple route to establishing recurrent structures without requiring an extrinsic pacemaker.  相似文献   

THE EFFECT OF X-RAYS ON POTATO TUBERS FOR "SEED"     

Sprague HB  Lenz M 《Science (New York, N.Y.)》1929,69(1797):606

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Determination of five macrolide antibiotic residues in raw milk using liquid chromatography-electrospray ionization tandem mass spectrometry     

Wang J  Leung D  Lenz SP 《Journal of agricultural and food chemistry》2006,54(8):2873-2880

A confirmatory method using liquid chromatography-electrospray ionization tandem mass spectrometry for determination of five macrolide antibiotics including spiramycin, tilmicosin, oleandomycin, erythromycin, and tylosin in raw milk is presented. Macrolides were extracted from raw milk by acetonitrile, and sample extracts were further cleaned up using solid-phase extraction cartridges. Data acquisition was achieved using multiple reaction monitoring, that is, two transitions, to provide a high degree of sensitivity and specificity. Matrix-matched standard calibration curves with the use of roxithromycin as an internal standard were utilized to achieve the best accuracy of the method. Both a conventional validation procedure and a designed experiment were applied to study the accuracy and precision of the method. The measurement uncertainty arising from accuracy and precision was estimated. The method accuracy, expressed as a percentage of overall recovery, was approximately 100%, and its intermediate precision was <10%. LC-ESI/MS/MS method detection limits (S/N > or = 3:1) of five macrolides were <0.3 microg/kg.  相似文献   

Effects of Growing Location and Irrigation on Attributes and Ethanol Yields of Selected Grain Sorghums     

X. Wu  R. Zhao  L. Liu  S. Bean  P. A. Seib  J. McLaren  R. Madl  M. Tuinstra  M. Lenz  D. Wang 《Cereal Chemistry》2008,85(4):495-501

Nine sorghum cultivars (five inbred lines and four hybrids) were grown in 2006 in three locations (Mount Hope, KS, Halstead, KS, and Plainview, TX) under different irrigation conditions and were evaluated for composition and ethanol fermentation efficiency. The objective was to study, in one growing season, the effects of genotype, growing location, and irrigation on the physical and chemical properties and fermentation efficiencies of grain sorghum. Genotype had a significant effect on chemical composition, physical properties, and ethanol yield. The cultivars showed a large variation in starch (61.0–74.8%), protein (7.56–16.35%), crude fat (2.79–4.77%), crude fiber (0.58–2.57%), ash (1.25–2.26%), kernel weight (20.0–35.9 mg), kernel hardness (49.6–97.5), and kernel size (1.9–2.7 mm) and were the most important factors affecting ethanol fermentation efficiency (87.5–93.9%). Starch and protein contents were significantly affected by growing location but not by irrigation. Environment had a significant effect on ethanol yields. Unexpectedly, irrigation somewhat reduced fermentation efficiency.  相似文献