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Locatelli C. Caronte I. Scaccabarozzi L. Migliavacca R. Pagani L. Moroni P. 《Veterinary research communications》2009,33(1):141-144
Veterinary Research Communications - 相似文献
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Giuliano Pisoni Patrizia D’Amelio Francesca Sassi Giovanni Manarolla Licia Scaccabarozzi Clara Locatelli Nausicaa Mazzocchi Mirko Baruscotti Dario DiFrancesco Paolo Moroni 《Veterinary journal (London, England : 1997)》2011,189(3):361-363
Formation of multinucleated giant cells (MGCs) by macrophage fusion is a typical cytopathic effect of lentiviral replication in caprine monocytes and MGC formation from cultured caprine peripheral blood mononuclear cells (PBMCs) has been considered to be diagnostic for small ruminant lentivirus (SRLV) infection. In this study, formation of MGCs was observed after 7–14 days when PBMCs were cultured from healthy goats free from SRLV infection. These MGCs expressed tartrate-resistant acid phosphatase, calcitonin receptor, integrin αVβ3, cathepsin K and matrix metalloproteinase 9 and were able to resorb bone in vitro in the absence of RANKL and macrophage colony stimulating factor, consistent with an osteoclast phenotype. 相似文献
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A. Harouna M. Zecchini C. Locatelli L. Scaccabarozzi C. Cattaneo A. Amadou V. Bronzo H. Marichatou P. J. Boettcher M. G. Zanoni L. Alborali P. Moroni 《Tropical animal health and production》2009,41(5):705-710
The prevalence of intra-mammary infections in dairy herds was studied in Hamdallaye, Niger. A total of 956 milk samples were
collected in 2007 from 239 lactating cows of four local breeds in eight traditional herds; the first sampling was undertaken
in the dry season at morning milking, and the second in the rainy season at evening milking. Staphylococcus aureus, Coagulase-Negative Staphylococci (CNS) and environmental microorganisms were detected in significantly (p < 0.05) more samples in the
rainy season, 55.2%, than in the dry season, 27.1%. Statistically significant (P < 0.05) differences in prevalence were observed
among herds and according to lactation number. Infections were assigned to four classes, according to the major pathogen,
and the respective mean somatic cell counts during the dry season were: S. aureus, 775 × 103 cells/ml; CNS, 447 × 103 cells/ml; environmental microorganisms, 407 × 103 cells/ml; and non-infected, 262 × 103 cells/ml. Most of the tested strains were sensitive to antibiotics, and selected strains of S. aureus (n = 15) were negative to the multiplex PCR tests for production of enterotoxins. 相似文献
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Caruso M Galgano F Castiglione Morelli MA Viggiani L Lencioni L Giussani B Favati F 《Journal of agricultural and food chemistry》2012,60(1):7-15
In this study the characterization of white wines produced from the monovarietal 'Greco bianco' grape variety is presented for the first time. A total of 40 commercial wines, from two different southern Italian regions, Calabria and Campania, from the same grape variety and two different vintages, were investigated. The analyses were performed by means of chromatographic methods, conventional analyses, and nuclear magnetic resonance (NMR) spectroscopy. No differentiation was observed according to the year of production but a significant discrimination was achieved using principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). In particular, PLS-DA allowed the selection of compounds (total acidity; citric, malic, succinic, and lactic acids; total polyphenol index; glucose and proline/arginine ratio) useful for differentiating the studied wines on the basis of geographical origin. 相似文献
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We explored the possibility of differentiating Italian wines produced in different regions by means of nuclear magnetic resonance (NMR) techniques. Ten commercial red Aglianico wines were selected from different areas of the Basilicata region in the south of Italy. Some important components of these wines were identified by the assignments of their (1)H and (13)C resonances using one- and two-dimensional homonuclear and heteronuclear NMR experiments. These data were compared with those obtained from 10 Aglianico wines produced in Campania, another southern Italian region. Differences were found among the wines according to their geographical origin and vintage. A fine discrimination of Aglianico wines from Basilicata and Campania was obtained, suggesting that the selected NMR parameters may be a valuable tool for wine authenticity control. 相似文献
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Lucchini B Ponti W Turin L Bronzo V Scaccabarozzi L Luzzago C 《Veterinary journal (London, England : 1997)》2012,192(1):126-128
The in vitro permissivity to infection with homologous and heterologous bovine viral diarrhoea virus (BVDV) strains of bovine peripheral blood mononuclear cells (PBMCs) from eight na?ve and eight BVDV-1b immune animals was studied. Four reference strains (BVDV-1a NADL, BVDV-1b NY-1, BVDV-2 125 and BVDV-2 890) were selected, based on genotype, prevalence and biotype. Virus neutralizing antibody titres were determined at bleeding and the viral loads were measured in PBMCs by end point titration in cell culture and by real-time PCR. PBMCs from both na?ve and immune animals became infected by all BVDV strains tested, although virus titres were lower for immune heifers than na?ve ones; the differences were significant for NADL (P<0.05) and 890 (P<0.001) strains. The in vitro model used in this study showed that PBMCs from immune animals are susceptible to re-infection with both homologous and heterologous BVDV strains, albeit at a lower extent than na?ve cattle. 相似文献
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Licia Scacco Carlo Bolaffio Antonio Romano Maurizio Fanciulli Alfonso Baldi Enrico Pierluigi Spugnini 《Journal of Equine Veterinary Science》2013
A 25-year-old gelding sport horse was referred for the treatment of recurring bilateral anal amelanotic melanoma. On physical examination, the horse presented bilateral poorly delimited perianal masses that recurred 2 months after surgical excision. The owner elected the lesions to be treated with surgery combined with electrochemotherapy using the drug cisplatin. The two masses were removed, and the surgical bed was treated with electrochemotherapy. A second session was performed 3 weeks later. The horse remained disease-free for 5 months and then experienced a marginal recurrence in one side. The nodule was re-treated, and the horse remained disease-free for 2 additional months, when it died of abdominal metastases. Electrochemotherapy can be added to the current strategies to palliate recurrent melanomas in horses. 相似文献
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