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AIM: To Study the influence of plasma TG level on the contents of serum HDL subpopulations. METHODS: Classified by the contents of serum TG, the serum level of HDL subpopulations in 106 normal TC and 183 high TC subjects were analyzed by two-dimensional gel electrophoresis coupled with immunodection method. RESULTS: The apo-AⅠcontents of per-β1-HDL, HDL3c, HDL3b and HDL3a were higher in a certain extent in TC high subjects vs corresponding TC desirable subjects. The apo-AⅠ contents of per-β1-HDL （in borer-line high TG subgroup） and HDL3b （in very high TG subgroup） were significantly higher (P<0.05 or P<0.01), while the apo-AⅠ contents of HDL2b and HDL2a were all lower in TC high subjects vs corresponding TC normal subjects. With the increase in plasma TG levels, the apoA-Ⅰ content of pre-β1-HDL increased, and it was significantly higher (P<0.05 or P<0.01) in borderline-high TG(except TC desirable subjects), high TG and very high TG subgroups vs corresponding normal TG subgroup. Contents of HDL3b and HDL3a had the same tendency, and in TC high subjects contents of HDL3b (in very high TG subgroup) and HDL3a (in every subgroups, in which levels of TG were higher) were significantly higher (P＜0.05 and P＜0.01） than in normal TG subgroup. On contrast, the apoA-Ⅰcontents of HDL2b and HDL2a following with the increase of plasma TG levels tended to become lower. Contents of HDL2b were significantly lower (P<0.05) in high TG subgroup and very high TG subgroup vs corresponding normal TG subgroup, and in TC high subjects contents of HDL2a were significantly lower (P<0.05) in very high TG subgroups vs normal TG subgroup. CONCLUSION: Our data show the general shift toward smaller size of HDL particle size with the increase in TC and TG levels. Contents of TC and TG are very important to contents of HDL subclasses. 相似文献
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【目的】 研究噻虫嗪与氯虫苯甲酰胺两种药剂,对非靶标害虫土耳其斯坦叶螨生长发育及种群参数的影响。【方法】 采用玻片浸渍法、叶碟法,研究土耳其斯坦叶螨对两种药剂的敏感性、生命参数变化。【结果】 经氯虫苯甲酰胺和噻虫嗪处理的土耳其斯坦叶螨成螨死亡率与对照均无明显差异;氯虫苯甲酰胺处理叶螨成螨后,次代除若Ⅱ期外,未成熟期的存活率均高于对照,而噻虫嗪处理时,次代若Ⅱ期的存活率高于对照,卵期、幼螨期和若Ⅰ期的存活率低于对照;经氯虫苯甲酰胺处理后,与对照和噻虫嗪处理相比,产卵前期明显缩短,世代显著延长,且日产卵量显著增多;氯虫苯甲酰胺处理的种群净增殖率R0 、内禀增长率rm 、周限增长率λ比对照和噻虫嗪处理增大,而施用噻虫嗪的叶螨平均世代历期T、种群加倍时间t比对照和施用氯虫苯甲酰胺延长。【结论】 氯虫苯甲酰胺有刺激土耳其斯坦叶螨增长的作用,而噻虫嗪无影响。 相似文献
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Yueh SC Wang YH Lin KY Tseng CF Chu HP Chen KJ Wang SS Lai IH Mao SJ 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(4):379-387
Porcine haptoglobin (Hp) is an acute phase protein. Its plasma level increases significantly during inflammation and infection. One of the main functions of Hp is to bind free hemoglobin (Hb) and inhibit its oxidative activity. In the present report, we studied the Hp phenotype of Taiwanese Lanyu miniature pigs (TLY minipigs; n=43) and found their Hp structure to be a homodimer (beta-alpha-alpha-beta) similar to human Hp 1-1. Interestingly, Western blot and high performance liquid chromatographic (HPLC) analysis showed that 25% of the TLY minipigs possessed low or no plasma Hp level (<0.05 mg/ml). The Hp cDNA of these TLY minipigs was then cloned, and the translated amino acid sequence was analyzed. No sequences were found to be deficient; they showed a 99.7% identity with domestic pigs (NP_999165). The mean overall Hp level of the TLY minipigs (0.21 +/- 0.25 mg/ml; n=43) determined by enzyme-linked immunosorbent assay (ELISA) was markedly lower than that of domestic pigs (0.78 +/- 0.45 mg/ml; p<0.001), while 25% of the TLY minipigs had an Hp level that was extremely low (<0.05 mg/ml). In addition, the initial recovery rate (first 40 min) in the circulation of infused fluorescein isothiocyanate (FITC)-Hb was significantly higher in the TLY minipigs with extremely low Hp levels than those with high levels. This data suggests that the low concentration of Hp-Hb complex is responsible for the higher recovery rate of Hb in the circulation. TLY minipigs have been used as an experimental model for cardiovascular diseases; whether they can be used as a model for inflammatory diseases, with Hp as a marker, remains a topic of interest. However, since the Hp level varies significantly among individual TLY minipigs, it is necessary to prescreen the Hp levels of the animals to minimize variation in the experimental baseline. The present study may provide a reference value for future use of the TLY minipig as an animal model for inflammation-associated diseases. 相似文献
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Performance, serum characteristics, carcase traits and lipid metabolism of broilers as affected by supplement of chromium picolinate. 总被引：3，自引：0，他引：3
1. This study investigated the effects of dietary supplementation with chromium picolinate on the performance, serum and carcase traits and lipid metabolism of broilers. In trial 1, 120 1-day-old broilers with an equal number of males and females were assigned at random to 4 groups with 3 replicates. Four treatments with dietary supplements of 0 (control), 800, 1600 and 3200 microg/kg of chromium picolinate were used. In trial 2, 6-week-old broilers (20) were used to determine how supplements of 0, 200, 400 and 800 microg/kg chromium in an incubation medium influence their hepatocyte lipogenic capacity and adipocyte lipolysis, in vitro. 2. Dietary supplements of 1600 and 3200 microg/kg chromium in broiler diets significantly increased food consumption (P<0.05); 1600 microg/kg markedly improved weight gain (P<0.05); 1600 and 3200 microg/kg groups showed increased liver lipid content (P<0.05). However, the abdominal fat content tended to decrease in these 2 groups. 3. Dietary supplements of 1600 and 3200 microg/kg of chromium decreased serum glucose and nonesterified fatty acids (NEFA) concentrations while increasing serum phospholipid content (P<0.05). Insulin concentration decreased only in birds receiving 3200 ppb chromium (P<0.05). Serum triacylglycerol (TG) clearance rate in chromium-supplemented groups was markedly enhanced (P<0.05). 4. In addition, chromium supplemented groups had increased serum HDL contents and also reduced serum VLDL and LDL contents (P<0.05). 5. Trial 2 indicated that lipogenesis from [U-14C]glucose by isolated hepatocytes was significantly enhanced by 400 ppb chromium (P<0.05). 6. The results from this study demonstrate that a supplement of 1600 microg/kg of chromium picolinate in the ration influences the growth, carcase, serum traits and lipid metabolism of broilers. 相似文献
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大豆（Glycine max (L.) Merr.）GmFTL3和GmFTL5基因具有很高的序列相似性,均参与大豆开花调节,但表达模式并不相同,本研究以启动子为突破口,分析这两个基因之间的表达差异。从大豆品种天隆1号中克隆GmFTL3和GmFTL5的启动子,构建载体pGmFTL3pro::GUS和pGmFTL5pro::GUS,转化拟南芥进行GUS染色分析。结果显示：无论长日条件还是短日条件,苗期和花器官中GmFTL3启动子均不驱动GUS基因在拟南芥中表达,而GmFTL5启动子则驱动GUS基因在拟南芥中表达,且在苗期的不同阶段呈现组织表达特异性。在花器官中,GmFTL5启动子驱动GUS基因主要在花萼、花瓣、花药和花粉粒中表达。实时定量结果显示,在大豆中,GmFTL3和GmFTL5基因均有表达。综上所述,大豆GmFTL3启动子在大豆中有活性但在拟南芥中无活性;GmFTL5启动子在大豆和拟南芥中均有活性,但表达可能存在差异; GmFTL5启动子在花器官中表达可能暗示GmFTL5基因与花器官发育有关。 相似文献
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Maya Matsunami Toshinori Matsunami Ikuko Kodama Atsushi Ogawa Kyoko Toyofuku Junko Ishikawa－Sakurai 《Plant Production Science》2016,19(1):173-180
This study aimed to characterize the physiological and morphological traits that are associated with adaptation to unflooded soil conditions in rice. Four indica rice cultivars (Puluik Arang, Badari Dhan, Shwe Nang Gyi, and Ratul), which were previously identified as highly or less adaptable to unflooded soil conditions, were grown under flooded and unflooded (soil water potential; －0.10 MPa) soil conditions. Water uptake was measured every day for three weeks, and then the leaf water potential, the stomatal conductance, the dry matter weight, shoot and root morphological traits were measured. Puluik Arang and Badari Dhan exhibited greater leaf area expansion and higher maintenance of root development under the unflooded condition than that by other cultivars. The leaf water potential and stomatal conductance of fully expanded highest leaf in Puluik Arang and Badari Dhan were not affected by unflooded soil regime. Leaf area and root morphological traits were significantly correlated with water uptake regardless of soil moisture regimes. These results suggested that Puluik Arang and Badari Dhan exhibited great water uptake capacity through physiological and morphological adaptation of shoot and root traits to unflooded condition, resulting in great biomass productivity under the condition. 相似文献
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2007～2008年,以云南省建水县酸石榴园为基地,采用Malaise网诱捕器对园内昆虫群落结构进行了调查,根据节肢动物群落中物种的营养和取食关系将总群落划分为天敌亚群落、害虫亚群落、中性昆虫亚群落,利用群落多样性指标对石榴不同生育期昆虫群落结构及其动态进行了分析比较。结果表明,从2007年5月～2008年9月,共诱捕获得昆虫纲12目75科375种1708头。物种丰富度、多样性指数和均匀度分别为5025,508,086。其中寄生性天敌有79种300头,捕食性天敌有46种162头,害虫有193种959头,中性昆虫有57种287头。害虫亚群落、天敌亚群落和中性昆虫亚群落物种丰富度分别为2796,2021,989,多样性指数分别为423,436,326。天敌昆虫群落与害虫群落及中性昆虫群落相对多度呈互补关系 相似文献
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Tsung Ming Tsao Yue Ming Chen Hwo Shuenn Sheu Shung Yao Zhuang Ping Hua Shao Hua Wen Chen Kai Shuan Shea Ming Kuang Wang Yen Horng Shau Kai Yin Chiang 《Journal of Soils and Sediments》2012,12(7):1054-1065
Purpose
Information on the physicochemical properties, mineral species and micromorphology of lateritic soils and gravel soil layers in paleo-environmental soil profile is severely lacking. Red soil profile of the Taoyuan terrace was employed to demonstrate its different extents of lateritic weathering. The objectives of this study were to compare the physicochemical properties of lateritic soils and gravel soil layers and identify using conventional and synchrotron X-ray diffraction (XRD) analyses mineral species in nanoparticles separated by automated ultrafiltration device (AUD) apparatus.Materials and methods
Soil samples were collected from paleo-environmental lateritic soils. Soil samples were examined using elemental analysis, conventional and synchrotron XRD analyses, high gradient magnetic separation, separation and collection of nanoparticles by AUD apparatus, and transmission electron microscopy (TEM).Results and discussion
The soil pH, redness index, quantities of free Al- and Fe-oxides (Ald and Fed), and clay content of lateritic soils are higher than those of gravel soil layers. Illite, kaolinite, gibbsite, quartz, goethite, and hematite were identified in clay fractions and nanoparticles by conventional and synchrotron XRD analyses. TEM images show presence of hematite nanoparticles on the surface coating of kaolinite nanoparticles and aggregated hematite nanoparticles overlapping the edge of a kaolinite flake in a size range of 4?C7?nm. Synchrotron XRD techniques are more straightforward and powerful than conventional XRD with random powder methods for identifying nanoparticles in red soils, particularly for illite, kaolinite, goethite, and hematite nanoparticles. According to chemical compositions of clay fractions and red soil features in the Taoyuan terrace, these red soils can be taken as lateritic red earths or red earths.Conclusions
This work suggests that physicochemical properties, mineral species, and micromorphology of red soil at all depths can shed light on the extent of paleo-environmental lateritic weathering. 相似文献10.
AIM:To study effects of tumour necrosis factor alpha(TNFα) and interleukin-10(IL-10) on human cytomegalovirus AD169 (HCMV AD169) infection in human embryonic lung fibroblasts (HEL),and the ability of the infected HEL to produce TNFα. We have attempted to understand the effect of cytokines in the immune of HCMV infection. METHODS:TNFα、IL-10 were added separately with different concentrations before 24 h HCMV infection of HEL to study the effect of TNFα and IL-10 on multiplication of HCMV. HCMV was incubated with HEL, TNFα in culture supernatants were measured at 4, 24, 48, 72, 96 h postinfection. The level of TNFα was measured by enzyme-linked immunosorbent assay( ELISA). RESULTS:The addition of TNFα with the concentration of 10-100 μg/L or IL-10 with the concentration of 1-10 μg/L before 24 h HCMV infection of HEL could remarkably inhibited the multiplication of HCMV in HEL. Level of TNFα didn't increase in infected cell supernatants. CONCLUSION:TNFα and IL-10 play an important role in the immune of HCMV infection. 相似文献