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Veterinary Research Communications - 相似文献
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S. Morandi M. Brasca R. Lodi L. Brusetti C. Andrighetto A. Lombardi 《Research in veterinary science》2010,88(3):427-435
The study concerns 130 Staphylococcus aureus strains isolated from different raw-milk dairy products (122 isolates) and human samples (eight isolates). Four different typing techniques were applied: biochemical profiles (Biolog GP), restriction fragment length polymorphism of coagulase gene (coaRFLP), random amplified polymorphic DNA (RAPD) and multilocus variable number tandem repeat analysis (MLVA). Moreover multiplex-PCR was used to study the distribution of genes encoding staphylococcal enterotoxins. The results of this study reveal marked genomic and phenotypic variability among the tested S. aureus. The considered techniques were all found useful for strain typing, but, based on discriminatory power as the key parameter of the typing system, MLVA and Biolog GP were found to be the most powerful techniques. The methods showed little concordance in terms of discerning the clusters of related strains. 相似文献
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Molecular changes in soy and wheat breads during storage as probed by nuclear magnetic resonance (NMR) 总被引：1，自引：0，他引：1
Addition of raw ground almond has been shown to improve loaf quality (e.g., loaf specific volume) of soy bread. To better understand the effects of almond addition to soy bread and to follow these through storage, nuclear magnetic resonance spectroscopy relaxation times and cross-relaxation experiments were performed. Spin-spin relaxation times of water protons were similar for the two soy breads, and therefore changes of water interactions with the other components of the soy breads (with and without almond) were not considered to be major contributors to the differences in loaf quality observed between these breads. Additionally, T2 values of water protons were found to have a similar decreasing trend during storage, especially up to day 3, for all of the products studied. On the other hand, during storage, lipid proton relaxation times exhibited only small changes in wheat and regular soy bread, whereas the soy-almond bread showed a major decrease of lipid proton mobility in particular after day 3 and up to day 10. These findings may indicate that, after a few days of storage, the lipid fraction contributes to better plasticization of the soy bread with almond, which can affect acceptability and storage stability of the final product. Thus, the higher amount of lipids introduced in the almond-enriched soy bread is likely to be responsible for the improved loaf quality and may significantly affect shelf stability of the soy-containing product. 相似文献
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Proverbio D Spada E Perego R Grieco V Lodi M Di Giancamillo M Ferro E 《Journal of the American Animal Hospital Association》2012,48(2):132-138
This report describes multiple endocrine neoplasia in a dog, which is a rare hereditary disorder characterized by the presence of two or more neoplasms of different endocrine tissues within a patient. A 14 yr old dog was evaluated for polyuria/polydipsia, polyphagia, and abdominal enlargement. Adrenal-dependent hyperadrenocorticism with concomitant left thyroid enlargement and a presumed abdominal metastatic lesion were diagnosed by an adrenocorticotropic hormone stimulation test, ultrasonography, and computed tomography. Trilostane therapy was initiated and resolved the clinical signs for 2 yr at which time the dog presented with left testicular enlargement. The dog was euthanized and was diagnosed with adrenocortical carcinoma, thyroid carcinoma, an abdominal mass compatible with a metastatic lymph node, and bilateral interstitial cell testicular adenomas. To the authors' knowledge, this is the first report to describe the concomitant association of these types of endocrine neoplasms in a dog. The concomitant presence of these neoplasms could represent a potential variant of multiple endocrine neoplasia; however, the presence of the interstitial cell testicular adenomas may have only been an incidental finding. If any of these tumors are diagnosed, veterinarians should perform a thorough clinical assessment to evaluate for the presence of additional endocrine neoplasms or hyperplasia. 相似文献
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Milk and dairy products are frequently contaminated with enterotoxigenic Staphylococcus aureus, which is often involved in staphylococcal food poisoning. The distribution of genes encoding staphylococcal enterotoxins (SE) in S. aureus isolated from bovine, goat, sheep and buffalo milk and dairy products was verified by the presence of the corresponding SE production. A total of 112 strains of S. aureus were tested for SE production by immuno-enzymatic (SEA-SEE) and reversed passive latex agglutination (SEA-SED) methods, while multiplex-PCR was applied for SE genes (sea, sec, sed, seg, seh, sei, sej and sel). Of the total strains studied, 67% were detected to have some SE genes (se), but only 52% produced a detectable amount of the classic antigenic SE types. The bovine isolates frequently had enterotoxin SEA, SED and sej, while SEC and sel predominated in the goat and sheep strains. The results demonstrated (i) marked enterotoxigenic S. aureus strain variations, in accordance with strain origin and (ii) the two methods resulted in different information but concurred on the risk of foodstuff infection by S. aureus. 相似文献
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