The Influence of Morphology,Follicle Size and Bcl‐2 and Bax Transcripts on the Developmental Competence of Bovine Oocytes     

THC De Bem  PR Adona  FF Bressan  LG Mesquita  MR Chiaratti  FV Meirelles  CLV Leal 《Reproduction in domestic animals》2014,49(4):576-583

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The evolutionary taxonomy of culture     

Lomax A  Berkowitz N 《Science (New York, N.Y.)》1972,177(4045):228-239

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Recombinant 47-kilodalton cytosol factor restores NADPH oxidase in chronic granulomatous disease   总被引：40，自引：0，他引：40  

K J Lomax  T L Leto  H Nunoi  J I Gallin  H L Malech 《Science (New York, N.Y.)》1989,245(4916):409-412

A 47-kilodalton neutrophil cytosol factor (NCF-47k), required for activation of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase superoxide (O2-.) production, is absent in most patients with autosomal recessive chronic granulomatous disease (AR-CGD). NCF-47k cDNAs were cloned from an expression library. The largest clone predicted a 41.9-kD protein that contained an arginine and serine-rich COOH-terminal domain with potential protein kinase C phosphorylation sites. A 33-amino acid segment of NCF-47k shared 49% identity with ras p21 guanosine triphosphatase activating protein. Recombinant NCF-47k restored O2-. -producing activity to AR-CGD neutrophil cytosol in a cell-free assay. Production of active recombinant NCF-47k will enable functional regions of this molecule to be mapped.  相似文献   

Hairballs in feedlot-raised calves     

RM. HERD  LG. COOK 《Australian veterinary journal》1989,66(11):372-373

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The toxicity of cyclopiazonic acid in weaned pigs     

L G Lomax  R J Cole  J W Dorner 《Veterinary pathology》1984,21(4):418-424

Five- to six-week-old crossbred pigs weighing 5 to 14 kg were given purified cyclopiazonic acid at dosages of 10, 1.0, 0.1, and 0.01 mg/kg body weight orally for 14 days. Clinical signs observed by day 7 in pigs given 10 mg/kg body weight were weakness, inactivity, anorexia, rough hair coats, and reduced body weights. These pigs also developed diarrhea during week 2 of the experiment. The pigs given 1.0 mg/kg body weight had rough hair coats and were moderately inactive during the second week of the experiment. At necropsy, lesions were observed only in pigs given 10 and 1.0 mg/kg body weight of cyclopiazonic acid. Lesions were gastric ulcers, mucosal hyperemia, and hemorrhage throughout the small and large intestine in pigs given 10 mg/kg body weight of cyclopiazonic acid. The pigs also had yellow, fibrononecrotic material in the lumen of the small intestine and pale livers. One pig given 1.0 mg/kg body weight had gastric ulceration. Microscopic lesions in pigs given 10 mg/kg body weight were necrotizing gastroenteritis, focal hepatocellular necrosis, hepatic peripheral lobular fatty change, and focal renal tubular nephrosis with focal suppurative tubulointerstitial nephritis. Pigs given 1.0 mg/kg body weight of cyclopiazonic acid had necrotizing gastritis and villous blunting in the jejunum and ileum.  相似文献   

Characterization of the horseweed (Conyza canadensis) transcriptome using GS‐FLX 454 pyrosequencing and its application for expression analysis of candidate non‐target herbicide resistance genes     

Yanhui Peng  Laura LG Abercrombie  Joshua S Yuan  Chance W Riggins  R Douglas Sammons  Patrick J Tranel  C Neal Stewart Jr 《Pest management science》2010,66(10):1053-1062

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Topical anaesthesia alleviates short-term pain of castration and tail docking in lambs     

S Lomax  H Dickson  M Sheil  PA Windsor 《Australian veterinary journal》2010,88(3):67-74

Objective To investigate the effect of a topical anaesthetic formulation on pain alleviation, wound healing and systemic levels of local anaesthetic actives in lambs undergoing castration and tail docking. Design Three placebo-controlled and/or randomised experiments were conducted using three groups of Merino lambs (n = 62, 68 and 19) undergoing routine castration and tail docking. Procedure Surgical castration, with either surgical or hot-iron tail docking, was performed with and without the application of topical anaesthetic (Tri-Solfen®) or placebo. The effects of this procedure were compared with those of rubber ring castration and tail docking, and of the handled but unmarked controls. Wound pain was assessed using calibrated Von-Frey monofilaments over a 4-h period, pain-related behaviour was assessed over 5 h, wound healing was assessed at 14 and 28 days, and the plasma levels of lignocaine and bupivacaine were determined. Results Rapid and up to 4 h primary hyperalgesia developed following surgical castration and tail docking in the untreated and placebo-treated lambs. It was absent in the castration wounds, and significantly reduced in the tail-docking wounds, of the treated lambs. Hot-iron docking was associated with mild and transient secondary hyperalgesia, which was abolished by the topical anaesthesia. There was a significant reduction in pain-related behaviours in treated lambs, which were not significantly different in their behaviour to the sham-operation handled controls. Plasma lignocaine and bupivacaine levels were below the toxic thresholds in all tested lambs. Conclusion Topical anaesthesia alleviates wound pain and significantly reduces pain-related behaviours in lambs undergoing surgical castration plus surgical or hot-iron tail docking, without a negative effect on wound healing or a risk of systemic toxicity.  相似文献   

Topical vapocoolant spray reduces nociceptive response to ear notching in neonatal piglets     

Sabrina Lomax  Evelyn Hall  Lauren Oehlers  Peter White 《Veterinary anaesthesia and analgesia》2018,45(3):366-373

Objective

To evaluate the efficacy of a vapocoolant spray (VS) to provide local anaesthesia for piglets during ear notching.

Study design

Randomized study.

Animals

Eleven 10 day old and forty 3–5 day old Large White piglets.

Methods

Temperature validation studies using thermocouples and a temperature data logger were conducted on dead and live tissue to determine optimal spray distance and duration to reduce tissue temperature to below 10 °C. A behavioural trial was conducted to assess efficacy for ear notching. Piglets were randomly assigned to one of four treatment groups: positive control (POS; n = 10), VS (n = 10), lignocaine (LIG; n = 10) and negative control (NEG; n = 10). Spray was administered to the margin of each ear, from a distance of 10 cm, and for a duration of 2 seconds immediately prior to ear notching. Behavioural observation was used to assess movement and vocal response, with responses categorized as no response (0) and response (1).

Results

Temperature and tissue validation studies indicated that a 2 second spray from 10 cm reduced tissue temperature to below 10 °C, and reduced response to stimulation of the skin with an 18 gauge needle (p < 0.001). There was a significant effect of treatment on response to ear notching (p < 0.001). The probability of a piglet to respond to ear notching was 98.7% for NEG piglets, compared with those treated with VS (5.3%), LIG (1.1%) and sham-notched piglets (0.9%).

Conclusions and clinical relevance

This study contributes new data on the pain of ear notching in piglets. The application of a topical VS prior to ear notching reduced the antinociceptive response of piglets to the procedure, similar to that of a local anaesthetic. Cryoanaesthesia presents a simple and effective option for reducing the pain response to this simple husbandry procedure.  相似文献   

Analysis of tidal breathing flow volume loop in dogs with tracheal masses     

KK Adamama-Moraitou  D Pardali  NN Prassinos  LG Papazoglou  D Makris  KI Gourgoulianis  N Papaioannou  TS Rallis 《Australian veterinary journal》2010,88(9):351-356

Objectives To investigate whether there are any changes in the tidal breathing flow volume loop (TBFVL) in calm, non-dyspnoeic dogs with intratracheal masses. Methods We compared 4 dogs with intratracheal masses (group 1) with 10 healthy dogs (group 2). Routine clinical and laboratory examinations of the dogs were unremarkable, except for episodic upper respiratory obstructive signs in the dogs in group 1. Lateral radiography of the neck and thorax showed that group 1 dogs had masses that appeared to protrude into the tracheal lumen. Tracheoscopy and surgery or necropsy was performed to confirm the presence of the mass. Arterial blood gas and TBFVL analysis was carried out in all dogs to assess respiratory status. Results The shape of the TBFVL for dogs in group 1 was narrower and ovoid compared with that for the group 2 dogs. Tidal volume and expiratory and inspiratory times were significantly reduced, whereas the respiratory rate was increased for dogs in group 1 compared with dogs in group 2. Arterial blood gas analysis was unremarkable for all dogs. Conclusions TBFVL is a non-invasive technique that is easy to perform and well tolerated by dogs. In the absence of abnormalities detected by routine diagnostic evaluations and arterial blood gas analysis in dogs with intratracheal masses, the TBFVL contributes to the definition of the physiologic status of the airways at the time of testing, and results suggests that these dogs breathe quite normally when they are calm and non-dyspnoeic.  相似文献   

The effect of dietary polyunsaturated fatty acids (PUFA) on infection with the nematodes Ostertagia ostertagi and Cooperia oncophora in calves     

Muturi KN  Scaife JR  Lomax MA  Jackson F  Huntley J  Coop RL 《Veterinary parasitology》2005,129(3-4):273-283

Diet-induced changes in the polyunsaturated fatty acid (PUFA) content of immune cells influences the immune phenotype that develops following infection. The aim of this study was to examine the effect of manipulating dietary PUFA supply on tissue fatty acids composition and immunity to a mixed infection with an abomasal and an intestinal nematode parasite in calves. Calves (n=24) were allocated into two treatment groups and fed 25 g/day of either fish oil (n-3 group) or a binary mixture of palm/rapeseed oil (normal group) as a supplement in milk replacer. Within each treatment group eight calves were infected with 2000 L3 Ostertagia ostertagi and Cooperia oncophora, three times per week for 8 weeks, the remaining calves were pair-fed uninfected controls. Faecal egg counts (FEC) were carried out twice weekly. At slaughter, the whole gut was removed intact for worm counts and tissue samples were taken for fatty acid analysis. Samples of abomasum, duodenum and mid-gut were also collected for immunohistological analysis. FEC were not significantly influenced by oil supplement but tended to remain higher in the palm/rapeseed oil-fed group (normal infected). The number of intestinal immature worms was significantly (p<0.05) higher in the n-3 group. Mucosal mast cell (MMC) and eosinophil numbers were significantly increased (p<0.05) by infection and were significantly lower (p<0.05) in the intestinal tissue of the fish oil supplemented and infected group (n-3 infected group). These results suggest that feeding an n-3 PUFA-rich supplement (fish oil) can influence cellular mediators of immunity to nematode infection. This is the first report of the establishment of patency and the subsequent development of immunity to a mixed infection with O. ostertagi and C. oncophora in calves undergoing early rumen development. The trend in the FEC, MMC and eosinophil numbers in the n-3 group suggests that decreasing the dietary n-6/n-3 PUFA ratio may be a worthwhile immunonutritional strategy for potentiating the immune response to nematode parasite infection in the calf.  相似文献