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The study indicated that intravenously administered DF32P was a suitable label for mink erythrocytes. The mean survival time of erythrocytes of 29 adult male mink labelled with diisopropyl phosphorofluoridate32P(DF32P) was 92.2 ± 4.1 (SEM) days. Three colour types were tested: standard dark, pastel and sapphire. No significant difference in erythrocyte life span relating to colour type was apparent in these animals. 相似文献
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Viral Plasmacytosis (Aleutian Disease) in Mink: VI. The Development of Positive Coombs Tests in Experimental Infections
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Coombs tests became positive coincident with the development of plasmacytosis in experimentally infected mink. These observations are discussed as possible evidence of an auto-immune phenomenon. Several mechanisms are suggested in which a virus as primary etiologic agent might promote the observed plasmacytosis, hypergammaglobulinemia, and erythrocyte sensitization. 相似文献
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Viral Plasmacytosis (Aleutian Disease) in Mink: IV. Cytoplasmic Glycoprotein Inclusions and Their Differentiation from the Viral Inclusions of Distemper 总被引:3,自引:2,他引:1
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Lars Karstad 《Canadian journal of veterinary research》1964,28(6):143-147
The periodic acid-Schiff (PAS) method was found to be a convenient stain for differentiating the specific intracytoplasmic inclusions of distemper from similar eosinophilic inclusions seen in plasmacytosis and occasionally in other disease conditions of mink. These latter inclusions stained strongly with PAS and appeared to be composed of glycoprotein. Although their significance is not known, it is probable that they represent abnormal globulins produced in great excess by the proliferating plasma cells. A pathogenetic hypothesis is offered. 相似文献
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Six different serological tests were used to examine Kenyan cattle sera for antibodies to the herpesvirus of malignant catarrhal fever. Significantly higher levels of indirect immunofluorescent antibody to early and late virus antigens and of complement fixing antibody were found in the sera of 13 naturally infected cattle than in 482 sera collected from four different groups of normal cattle. Virus neutralising and immunoprecipitating antibodies were also found in some infected cattle sera but not in normal cattle sera. Many non-specific reactions occurred using counterimmunoelectrophoresis. These preliminary results indicate that the serological diagnosis of wildebeest-associated malignant catarrhal fever may be possible. 相似文献
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The effects of the herbicide, DNOC, 4,6-dinitro-o-cresol, a model pollutant, have been studied by comparing the potential of soil microbial communities present in individual soil aggregates or in larger soil microcosms as samples of soil aggregates to mineralise the herbicide 2,4-D. We have shown that 2-3 mm soil aggregates vary widely in their 2,4-D mineralisation potential and that ageing or exposure to DNOC considerably simplified the distribution patterns of this capacity. The main factors of variation have been quantified and classified using a quasi-likelihood method derived from the Generalised Linear Model approach. Besides DNOC concentration and duration of exposure, an additional ‘rank’ factor reflecting a desiccation gradient of the aggregates on the microtiter plates was found to have statistical significance. We concluded that it should be possible to derive an experimental approach, designated as ‘functional profiling’, with potential use to detect soil chemical contamination. Curves of 2,4-D mineralisation in individual soil aggregates could be classified according to three different types of kinetics, which were assumed to reflect heterogeneous spatial distribution, differences in microbial community composition and varying efficiency of the microbial consortia involved in 2,4-D degradation. Exposure to DNOC considerably simplified the distribution patterns of the different types of kinetics with one type, showing slow rate and low cumulative mineralisation, becoming predominant as ageing, concentration and duration of DNOC exposure increased. We argue on the possible use of ‘kinetic profiling’ as a sensitive bioindicator of soil quality. By comparison, in soil microcosms, 2,4-D mineralisation showed an extra mineralisation potential of 64% over individual aggregates in the control soil and exposure to DNOC was followed by concentration and time-dependent recovery of the 2,4-D mineralisation potential. It is likely that 2 g size soil microcosms gather a larger number of biochemical capacities which could complement each other to increase the potential of soil to mineralise xenobiotic compounds. 相似文献