Surgical implantation of temperature‐sensitive transmitters and data‐loggers to record body temperature in koalas (Phascolarctos cinereus)          下载免费PDF全文

D Adam  SD Johnston  L Beard  V Nicholson  A Lisle  J Gaughan  R Larkin  P Theilemann  A Mckinnon  W Ellis 《Australian veterinary journal》2016,94(1-2):42-47

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Plasma lipopolysaccharide elevations in cattle associated with early-stage infection by Fasciola hepatica     

D Marendy  L Gabor  SD George  A Parker  E Doyle 《Australian veterinary journal》2023,101(9):334-338

Fasciolosis is an endemic zoonotic parasitic disease with significant impacts on human health and both animal health and production. Early post-infection impacts on the host remain unclear. The objective of this study was to determine the changes, if any, to levels of endotoxin in cattle plasma in response to early-stage infection with Fasciola hepatica. Thirty-six (36) commercial bred cattle were experimentally infected with approximately 400 viable metacercariae. Plasma lipopolysaccharide (endotoxin) levels were examined on 24 occasions from 0 h before infection to 336 h after infection using the Limulus Amoebocyte Lysate chromogenic end point assay and compared with that of six (6) uninfected control animals. Peak lipopolysaccharide levels in infected animals were reached at 52 h after infection and returned to pre-infection levels at time 144 h after infection. Infected animals had significantly elevated lipopolysaccharide levels between 24 and 120 h after infection when compared to uninfected animals. The mean change in endotoxin units (EU)/mL over time after infection was statistically significant in infected animals. Elevations of lipopolysaccharide occurred in all infected animals suggesting a possible repeatable and titratable endotoxemia conducive to therapeutic agent model development.  相似文献   

Matrix metalloproteinase activity in tumor, stromal tissue, and serum from cats with malignancies     

Jankowski MK  Ogilvie GK  Lana SE  Fettman MJ  Hansen RA  Powers BE  Mitchener KM  Lovett SD  Richardson KL  Parsley L  Walton JA 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(1):105-108

Matrix metalloproteinases (MMPs) are enzymes that play key roles in angiogenesis, tumor invasion, and metastasis in a wide variety of species. The purpose of this study was to evaluate pro and active MMP 2 and 9 concentrations in tumor, normal stromal tissue, and serum from tumor-bearing cats. We hypothesized that serum concentrations of pro and active forms of MMPs 2 and 9 would be predictive of MMP concentrations in tumor tissue and that these MMP concentrations would correlate with the histopathologic grade of the malignancies. Pro and active forms of MMPs 2 and 9 were determined by gelatin zymography and subsequent computerized densitometry from tumor and nearby stromal tissue and serum from 49 cats with various malignancies. The serum concentrations of MMPs from these tumor-bearing cats were compared with serum concentrations of MMPs from 44 normal cats of similar age and gender. Measurable concentrations of MMPs 2 and 9 were found within tumor, stromal, and serum samples. Mean concentrations of total pro and active MMPs 2 and 9 within tumor tissue were significantly higher (P values <.0001, .0031, <.001, and .0064, respectively) when compared with stromal tissue from the same animals. Serum MMP concentrations from tumor-bearing cats were higher than those from normal cats. Poor correlation was found between serum MMP concentrations and tissue MMP concentrations of increasing histologic grades of malignancies.  相似文献   

Bluetongue virus serotypes 1 and 3 infection in Poll Dorset sheep   总被引：1，自引：0，他引：1  

C. HAMBLIN  JS SALT  SD GRAHAM  K. HOPWOOD  AM WADE-EVANS 《Australian veterinary journal》1998,76(9):622-629

Objective To study the clinical signs following bluetongue virus serotypes 1 and 3 infection in Poll Dorset sheep.
Design A clinical and pathological study.
Procedure Twenty Poll Dorset sheep were inoculated with bluetongue virus serotypes 1 or 3, each inoculum having a different passage history. The sheep were examined daily and their clinical appearance and rectal temperatures recorded. Heparinised and non-heparinised blood samples were taken at intervals for virological and serological study. Gross pathological findings were recorded for several sheep at necropsy and tissue samples were collected from three sheep for virological studies.
Results All inoculated sheep developed clinical disease. The clinical signs and gross pathological changes varied considerably but were consistent with damage to the vascular endothelial system. There was a decline in the titres of infectious bluetongue virus and of antigen in tissues collected between 7 and 12 days after infection.
Conclusions The severity of disease was related to the speed of onset and duration of pyrexia and not the development or titre of viraemia. Generally, those animals with sensitive mouths, depression, coronitis, recumbency and reluctance to move were the most debilitated. Whole blood was the most reliable source of infectious virus from acutely and chronically infected and convalescent animals. However, tissue samples particularly spleen, collected from dead or killed animals suffering from either peracute or acute forms of disease were most appropriate for the rapid confirmation of a clinical diagnosis.  相似文献   

THE ELECTRONIC MEASUREMENT OF SMALL HERBAGE YIELDS USING A HIGH SENSITIVITY CAPACITANCE METER     

J. V. Lovett  G. J. Burchi 《Grass and Forage Science》1972,27(2):83-86

The capacitance meters available in the past for estimating herbage yields have lacked sensitivity at the extremes of their range of measurement. The preliminary evaluation of a highly sensitive capacitance meter described here indicates that accurate measurement is possible in the range 0.1100 kg DM/ha. The implications of this development for the measurement of herbage of low availability are discussed.  相似文献   

Effect of Oil Overlay on Inhibition Potential of Roscovitine in Sheep Cumulus‐Oocyte Complexes          下载免费PDF全文

LF Crocomo  WC Marques Filho  CMV Ulian  NS Branchini  DT Silva  CL Ackermann  FC Landim‐Alvarenga  SD Bicudo 《Reproduction in domestic animals》2015,50(3):410-416

Inhibitors of cyclin‐dependent kinases, as roscovitine, have been used to prevent the spontaneous resumption of meiosis in vitro and to improve the oocyte developmental competence. In this study, the interference of oil overlay on the reversible arrest capacity of roscovitine in sheep oocytes as well as its effects on cumulus expansion was evaluated. For this, cumulus‐oocyte complexes (COCs) were cultured for 20 h in TCM 199 with 10% foetal bovine serum (Control) containing 75 μm roscovitine (Rosco). Subsequently, they were in vitro matured (IVM) for further 18 h in inhibitor‐free medium with LH and FSH. The culture was performed in Petri dishes under mineral oil (+) or in 96 well plates without oil overlay (?) at 38.5°C and 5% CO₂. At 20 and 38 h, the cumulus expansion and nuclear maturation were evaluated under stereomicroscope and by Hoechst 33342 staining, respectively. No group presented cumulus expansion at 20 h. After additional culture with gonadotrophins, a significant rate of COCs from both Control groups (+/?) exhibited total expansion while in both Rosco groups (+/?) the partial expansion prevailed. Among the oocytes treated with roscovitine, 65.2% were kept at GV in the absence of oil overlay while 40.6% of them reached MII under oil cover (p < 0.05). This meiotic arrest was reversible, and proper meiosis progression also occurred in the Control groups (+/?). So, the culture system without oil overlay improved the meiotic inhibition promoted by roscovitine without affecting the cumulus expansion rate or the subsequent meiosis progression.  相似文献   

Isolation and identification of Listeria monocytogenes in dairy products   总被引：12，自引：0，他引：12  

J Lovett 《Journal of the Association of Official Analytical Chemists》1988,71(3):658-660

After an outbreak of listeriosis in Massachusetts in 1983, the ability of Listeria monocytogenes to survive in raw and pasteurized milk was investigated. An enrichment broth (EB) containing acriflavine, nalidixic acid, and cycloheximide was used to eliminate overgrowth of the culture by competing organisms, and a modification of McBride's agar (MMA) was used as the isolation medium. The culture was incubated 24 h at 30 degrees C. To isolate Listeria from soft cheese, the incubation period was lengthened to 1 week, and the EB culture was streaked to MMA at 1 and 7 days. Physical and biochemical patterns, the CAMP test, serological tests, and mouse pathogenicity studies were helpful in determining the identity of L. monocytogenes.  相似文献   

PLANETARY SCIENCE: 'Spiders' Channel Mars Polar Ice Cap     

Lovett RA 《Science (New York, N.Y.)》2000,289(5486):1853a-1854a

Scientists studying the latest high-resolution photos of the martian south polar ice cap think they may have found additional clues to its ebb and flow. These hints of the planet's bizarre atmosphere come from a new class of dramatic-looking terrain features whose dark, multilimbed, vaguely radial designs have earned them the moniker "black spiders," and another group of dusky, spreading features called "dark fans." At a recent gathering here of Mars researchers, a planetary scientist proposed that the spiders might be subsurface gas channels, visible through an unusually transparent section of the martian ice.  相似文献   

Global warming. Rain might be leading carbon sink factor     

Lovett RA 《Science (New York, N.Y.)》2002,296(5574):1787

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Enzyme immunoassay for screening sulfamethazine residues in swine blood   总被引：12，自引：0，他引：12  

J R Fleeker  L J Lovett 《Journal of the Association of Official Analytical Chemists》1985,68(2):172-174

An enzyme immunoassay (EIA) was developed to screen for residues of sulfamethazine (SMT) and its metabolites in swine blood. Swine blood was treated with perchloric acid and centrifuged. The supernatant solution was neutralized with K2HPO4, centrifuged, and applied to a reverse phase C8 cartridge. The analytes were eluted with methanol-water (2 + 3), and the eluate was diluted and assayed. Average recoveries, using 14C-labeled compounds, were 73, 72, 61, and 62% for SMT, N4-glucosylSMT, N4-acetylSMT, and N4-desaminoSMT, respectively. Tubes coated with antibody were incubated with the eluate and an SMT-beta-galactosidase conjugate. Bound enzyme was detected with fluorogenic substrate. When blood was fortified with 0.1 ppm SMT or a molar equivalent of metabolite, the average relative response of the EIA was 100%, control blood; 61%, SMT; 66%, glucosylSMT; 60%, acetylSMT; and 77% desaminoSMT.  相似文献