心脏体外循环系统检测植物外啡肽活性的研究     

孙镇平  华棣  吕立力  熊万斌  周业飞 《猪业科学》2003,20(7):71-72

本文利用猪胃蛋白酶在37℃条件下降解中筋粉,采集不同反应时间的酶解液样品,低温离心处理后,分别加入蛙心体外循环系统中,测定心肌收缩力变化,并随即加入阿片肽阻断剂-纳洛酮,检测植物外啡肽活性。结果表明,酶解液能够引起心肌收缩力加强,并可被纳洛酮逆转,证明酶解液具有植物外啡肽活性。  相似文献   

乳清清凉饮料的研制--干酪副产物乳清的利用     

史容  朱芊娣 《乳业科学与技术》2002,25(1):12-14

通过对稳定剂的稳定效果、糖酸比例、工艺条件等几个方面的研究,表明乳清清凉饮料的最佳条件:稳定剂含量为0.25％,白砂糖10.0％,采用90-95℃,5min杀菌,发酵温度为42±0.5℃,时间5.5h。  相似文献   

犬瘟热病毒的静水压灭活   总被引：4，自引：1，他引：3  

池元斌  夏咸柱  王雪梅  余春  王琰第  何洪彬  范泉水  黄耕  王允海  刘海涛  张春红 《中国兽医学报》2001,21(1):35-37

以250MPa的静水压力对犬瘟热病毒（CDV）处理30min之后，CDV被灭活，由其制成的灭活疫苗诱导犬产生中和抗体的能力明显优于传统的福尔马林灭活疫苗，免疫保护率达90％。电镜观察显示，经不同程度静水压力处理后的CDV粒子均发生变形，表面呈现凸起，但CDV H基因部分片段的RT－PCR扩增结果揭示，高达510MPa的静水压力作用30min也未能使其基因片段受到破坏。  相似文献   

Haematological and biochemical investigations of diseased koalas (Phascolarctos cinereus)     

PJ CANFIELD  ME O'NEILL†  EF SMITH† 《Australian veterinary journal》1989,66(9):269-272

Haematological and biochemical investigations were performed on 14 koalas with uncomplicated cystitis, 8 with complicated cystitis, 8 with conjunctivitis, 8 with lymphosarcoma, and 14 with miscellaneous diseases. Changes were limited and inconsistent in individual koalas with uncomplicated cystitis and conjunctivitis. In contrast, individual koalas with complicated cystitis were more likely to have anaemia, leukocytosis due to neutrophilia, hypoproteinaemia due to hypoalbuminaemia, and azotaemia due to elevated urea concentration. Although these changes were non-specific they did allow assessment of prognosis for survival and response to treatment. Koalas with lymphosarcoma were invariably anaemic, leukaemic, azotaemic and hypoalbuminaemic. Elevated enzymes (aspartate transaminase [AST]. lactate dehydrogenase [LD] and gamma glutamyl transferase [GGT]) were more common in koalas with lymphosarcoma. Koalas affected by miscellaneous conditions showed variable changes but once again anaemia, leukocytosis, azotaemia, elevated AST and LD, and hypoalbuminaemia were not uncommon. On the basis of these findings a minimal profile is suggested for the investigation of sick koalas and would include haematocrit, total and differential leukocyte counts, urea, total protein and albumin concentrations and AST, GGT and LD activities.  相似文献   

Salmonella serotypes isolated in geckos kept in seven collections in southern Italy          下载免费PDF全文

T. P. Russo  L. Varriale  L. Borrelli  A. Pace  M. Latronico  L. F. Menna  A. Fioretti  L. Dipineto 《The Journal of small animal practice》2018,59(5):294-297

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Effect of resistant starch on the intestinal health of old dogs: fermentation products and histological features of the intestinal mucosa          下载免费PDF全文

M. C. Peixoto  É. M. Ribeiro  A. P. J. Maria  B. A. Loureiro  L. G. di Santo  T. C. Putarov  F. N. Yoshitoshi  G. T. Pereira  L. R. M. Sá  A. C. Carciofi 《Journal of animal physiology and animal nutrition》2018,102(1):e111-e121

The effects of resistant starch (RS) intake on nutrient digestibility, microbial fermentation products, faecal IgA, faecal pH, and histological features of the intestinal mucosa of old dogs were evaluated. The same formulation was extruded in two different conditions: one to obtain elevated starch cooking degree with low RS content (0.21%) and the other lower starch cooking with high RS content (1.46%). Eight geriatric Beagles (11.5 ± 0.38 years old) were fed each diet for 61 days in a crossover design. Food intake, nutrient digestibility, fermentation products, faecal pH, and faecal IgA were examined via variance analysis. Histological results of intestinal biopsies were assessed via Wilcoxon test for paired data. The morphometric characteristics of large intestine crypts were evaluated via paired t tests (p < .05). Protein, fat, and energy digestibilities were higher for the low‐RS diet (p < .05). Dogs receiving the high‐RS diet had lower faecal pH and higher values for propionate, butyrate, total volatile fatty acids, and lactate (p < .05). No differences between diets were found in the histological parameters of the gut mucosa, and only a tendency for deeper crypts in the descending colon was observed for dogs fed the high‐RS diet (p = .083). The intake of a corn‐based kibble diet manufactured with coarse ground raw material and low starch gelatinization to obtain 1.4% of RS affected microbial fermentation products and faecal pH and tended to increase crypt depth in the descending colon of old dogs.  相似文献   

Effects of Clinical Mastitis on Ovarian Function in Post-partum Dairy Cows     

Gy Huszenicza  Sz Jánosi  M Kulcsár  P Kóródi  J Reiczigel  L Kátai  AR Peters  F De Rensis 《Reproduction in domestic animals》2005,40(3):199-204

Mastitis-induced ovarian abnormalities were studied in a field trial. At 1-3 day after calving, > or = 2 parity cows not affected with chronic recurrent mastitis and yielding < 400,000/ml somatic cell count (SCC) individual milk in the previous lactation, were enrolled in the study. Thereafter milk samples were collected three times weekly for 95-100 day for progesterone (P4) assay. Individual P4 profiles were used to monitor ovarian cyclicity. When mastitis was diagnosed in the first 80 day post-partum (pp), clinical signs were recorded and scored, and aseptic milk samples were taken to identify the mastitis pathogens. Depending on the isolated pathogens the cows were blocked into one of the three sub-groups affected by either Gram-positive (GP), or Gram-negative (GN) bacteria, or of those with no detected pathogens (NDP). Cows suffering from any type of mastitis between days 15 and 28 (n = 27) showed a delay in the onset of ovarian cyclicity, and estrus was postponed compared to cows affected during the first 14 day pp (n = 59) and controls (n = 175) (38.6 +/- 2.3 vs 33.4 +/- 2.1 and 32.0 +/- 1.0 day, respectively, for onset of ovarian cyclicity and 90.7 +/- 2.5 vs 80.2 +/- 2.8 and 83.9 +/- 2.1 day, respectively, for estrus; both p < 0.05). The percentage of cows ovulating by day 28 was lower in those affected by mastitis between days 14 and 28 compared to cows between days 1 and 14 and controls (22.2% vs 47.5 and 50.3%, respectively; p < 0.05). A significantly higher rate of premature luteolysis was observed in GN + NDP compared to GP mastitis and healthy cows (46.7% vs 8.3 and 2.0%, respectively; p < 0.001). If the mastitis outbreak occurred during the follicular phase, the duration of this cycle segment was lengthened in GN + NDP mastitis compared to GP mastitis and healthy cows (10.8 +/- 0.9 vs 7.9 +/- 0.1 and 7.2 +/- 0.1, respectively; p < 0.001). The results indicate that mastitis can affect the resumption of ovarian activity in pp dairy cows. Mastitis may also impair reproduction also in cyclic cows: this effect can be the consequence of premature luteolysis or a prolonged follicular phase.  相似文献   

应用RT-PCR方法检测水稻植株和介体昆虫体内的水稻齿叶矮缩病毒   总被引：3，自引：0，他引：3  

雷娟利  吕永平  金登迪  陈声祥  陈剑平 《植物病理学报》2001,31(4):306-309

 将生物学接种和RT-PCR检测水稻植株体内的水稻齿叶矮缩病毒的方法进行了比较,发现结果基本趋于一致,但总体上RT-PCR检测阳性率稍高于生物学接种的发病率。将生物学接种检测褐飞虱介体内水稻齿叶矮缩病毒方法与RT-PCR检测方法进行比较分析,发现RT-PCR方法可以检测单头褐飞虱体内的水稻齿叶矮缩病毒,具有很高的准确性和灵敏度。  相似文献   

The identification of Mortierella wolfii isolated from cases of abortion and pneumonia in cattle and a search for its infection source     

M E Di Menna  M E Carter  D O Cordes 《Research in veterinary science》1972,13(5):439-442

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Experimental field trial with an immunostimulating complex (ISCOM) vaccine against contagious bovine pleuropneumonia     

Hübschle OJ  Tjipura-Zaire G  Abusugra I  di Francesca G  Mettler F  Pini A  Morein B 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(6):298-303

The experimental field trial with an immunostimulating complex (ISCOM) vaccine has been an occasion to explore the role of a Th1 response in the pathogenesis caused by Mycoplasma mycoides subsp. mycoides small colony (MmmSC) and in immune protection. The ISCOM complex is known to promote Th1 response. Antibodies to MmmSC were detected by indirect enzyme-linked immunosorbent assay (ELISA) in the vaccinated cattle, although the levels were lower than in a previous study. No antibodies were detected by complement fixation test (CF). After the challenge infection, vaccinated animals developed CF antibody response. They showed significantly reduced mortality compared with controls. However, gross pathological and histopathological score for vaccinated animals was as high as for the non-vaccinated, characterized by a high inflammatory reaction with histopathology dominated by interlobular pneumonia with vasculitis.  相似文献