Analysis of milk-derived isolates of E. coli indicating drug resistance in central Ethiopia     

Messele  Yohannes Equar  Abdi  Reta Duguma  Tegegne  Desiye Tesfaye  Bora  Shelema Kelbesa  Babura  Mosisa Dire  Emeru  Bezina Arega  Werid  Gebremeskel Mamu 《Tropical animal health and production》2019,51(3):661-667

Mastitis is one of the most important diseases in dairy cows throughout the world and is responsible for significant economic losses to the dairy industry. This study was performed to characterize the genetic basis of drug resistance in Escherichia coli isolated from cases of clinical and sub-clinical bovine mastitis. A total of 224 California mastitis test (CMT)-positive milk samples were collected from December 2015 to April 2016 to characterize the phenotypic and genetic basis of antimicrobial resistance in E. coli isolated from raw milk from dairy farms found in Burayu, Sebeta, and Holeta areas of Ethiopia. The prevalence of E. coli was 7.1% (16) and both phenotypic and molecular techniques were used to identify E. coli antimicrobial susceptibility trait. The most commonly observed phenotypic resistance was against ampicillin (68.7%), sulphamethazole-trimethoprim (50%), and streptomycin (25%). Multidrug resistance phenotypes were found in 11 of 16 (68.7%) of E. coli isolates. Tetracycline (tet (A)) and chloramphenicol (cml (A)) genes were the most predominant encoding resistance genes identified (50%) each, followed by gentamycin resistance encoding gene (aac (3)-IV) (37.5%). Overall, 11 (68.7%) of the isolates had multidrug resistance genes responsible to two or more classes of antibiotics. The most common pattern detected was cml (A) and tet (A) together 37.5% followed by aac (3)-IV and tet (A) 25%. The current study indicated that raw milk could be regarded as critical source of antibiotic-resistant pathogenic E. coli.

  相似文献   

Prognostic value of serum cystatin C concentration in dogs with myxomatous mitral valve disease     

Naoki Iwasa  Rie Kumazawa  Saki Nomura  Mamu Shimizu  Munetaka Iwata  Mayuka Hara  Mifumi Kawabe  Yui Kobatake  Satoshi Takashima  Naohito Nishii 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(2):412-419

  相似文献   

Effect of age,sex, and breed on serum cystatin C and creatinine concentrations in dogs     

Iwasa  Naoki  Takashima  Satoshi  Iwasa  Tatsuo  Kumazawa  Rie  Nomura  Saki  Asami  Sara  Shimizu  Mamu  Kobatake  Yui  Nishii  Naohito 《Veterinary research communications》2022,46(1):183-188

Veterinary Research Communications - This study investigated the effects of age, sex and breed on serum cystatin C (Cys-C) and creatinine in small breed dogs. This retrospective study included 250...  相似文献