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1.
Mandeep Atray Mulinti Raghunath Tarunbir Singh Narinder Singh Saini 《The Canadian veterinary journal. La revue veterinaire canadienne》2012,53(8):860-864
The diagnosis and treatment of double intestinal intussusception in 3 pups with persistent vomiting, diarrhea, dehydration, anemia, leucocytosis, and electrolyte imbalance are described. Ultrasonography confirmed intussusception and laparotomy revealed double intussusceptions. Intussusceptions were corrected by manual reduction in 1 pup and intestinal resection and anastomosis in 2 pups. Two pups survived and 1 pup died on the 4th day after surgery. 相似文献
2.
Subhash Verma Mandeep Sharma Shailja Katoch Lovit Verma Sandeep Kumar Vishal Dogra Rajesh Chahota Prasenjit Dhar Geetanjali Singh 《Veterinary research communications》2013,37(1):83-89
Pasteurella multocida is a causative agent of many major diseases of which haemorrhagic septiciemia (HS) in cattle & a buffalo is responsible for significant losses to livestock sector in India and south Asia. The disease outcome is affected by various host- and pathogen-specific determinants. Several bacterial species-specific putative virulence factors including the capsular and virulence associated genes have been proposed to play a key role in this interaction. A total of 23 isolates of P. multocida were obtained from 335 cases of various clinically healthy and diseased cattle. These isolates were examined for capsule synthesis genes (capA, B, D, E and F) and eleven virulence associated genes (tbpA, pfhA, toxA, hgbB, hgbA, nanH, nanB, sodA, sodC, oma87 and ptfA) by PCR. A total of 19 P. multocida isolates belonging to capsular type B and 4 of capsular type A were isolated. All isolates of capsular type B harboured the virulence associated genes: tbpA, pfhA, hgbA, sodC and nanH, coding for transferrin binding protein, filamentous hemagglutinin, haemoglobin binding protein, superoxide dismutase and neuraminidases, respectively; while isolates belonging to capsular type A also carried tbpA, pfhA, hgbA and nanH genes. Only 50 % of capsular type A isolates contained sodC gene while 100 % of capsular type B isolates had sodC gene. The gene nanB and toxA were absent in all the 23 isolates. In capsular type A isolates, either sodA or sodC gene was present & these genes did not occur concurrently. The presence of virulence associated gene ptfA revealed a positive association with the disease outcome in cattle and could therefore be an important epidemiological marker gene for characterizing P. multocida isolates. 相似文献
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Sridhar BHAVANI David P. HODSON Julio HUERTA-ESPINO Mandeep S. RANDHAWA Ravi P. SINGH 《农业科学与工程前沿(英文版)》2019,6(3):210
Races belonging to the Ug99 (TTKSK) lineage of the wheat stem rust fungus, carrying complex virulence combinations, and their migration to countries in Africa, Middle East and Asia continue to pose a significant threat to global wheat production. The rapid spread of additional races, e.g., TKTTF or the Digalu lineage, in several countries causing localized epidemics reminds us of the vulnerability of wheat germplasm to stem rust disease, a formidable foe referenced as early as biblical times. A global rust monitoring system reflecting increased surveillance efforts has identified 13 races within the Ug99 lineage in 13 countries and unrelated lineages are emerging, spreading and posing serious threats to wheat production. Race TKTTF has caused localized epidemics in Ethiopia and its variants have been recently implicated in stem rust outbreaks in Europe. Concerted research efforts have resulted in the identification of several new resistance genes and gene combinations for use in breeding. Combining multiple adult plant resistance (APR) genes in high-yielding backgrounds and discovery of new quantitative trait loci conferring stem rust resistance has progressed in the recent years, enhancing the durability of resistance. Effective gene stewardship and new generation breeding materials and cultivars that combine multiple race-specific or minor to intermediate effect APR genes, complemented by active surveillance and monitoring, have helped to limit major epidemics and increase grain yield potential in key target environments. 相似文献
4.
Development of a continuous cell line from larval Atlantic cod (Gadus morhua) and its use in the study of the microsporidian,Loma morhua 
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下载免费PDF全文 Michael J. MacLeod Nguyen T. K. Vo Michael S. Mikhaeil S. Richelle Monaghan J. Andrew N. Alexander Mandeep K. Saran Lucy E. J. Lee 《Journal of fish diseases》2018,41(9):1359-1372
In vitro cell culture methods are crucial for the isolation, purification and mass propagation of intracellular pathogens of aquatic organisms. Cell culture infection models can yield insights into infection mechanisms, aid in developing methods for disease mitigation and prevention, and inform commercial‐scale cultivation approaches. This study details the establishment of a larval cell line (GML‐5) from the Atlantic cod (Gadus morhua) and its use in the study of microsporidia. GML‐5 has survived over 100 passages in 8 years of culture. The line remains active and viable between 8 and 21°C in Leibovitz‐15 (L‐15) media with 10% foetal bovine serum and exhibits a myofibroblast phenotype as indicated by immuno‐positive results for vimentin, α‐smooth muscle actin, collagen I and S‐100 proteins, while being desmin‐negative. GML‐5 supports the infection and development of two microsporidian parasites, an opportunistic generalist (Anncaliia algerae) and cod‐specific Loma morhua. Using GML‐5, spore germination and proliferation of L. morhua was found to require exposure to basic pH and cool incubation temperatures (8°C), in contrast to A. algerae, which required no cultural modifications. Loma morhua‐associated xenoma‐like structures were observed 2 weeks postexposure. This in vitro infection model may serve as a valuable tool for cod parasitology and aquaculture research. 相似文献
5.
Mass rearing for commercial production of high quality beneficial insects is considered as an important tool for biological control programmes worldwide, especially those based on augmentative releases. Low temperature storage is a valuable method for increasing the shelf life of entomophagous insects. Insect predators and parasitoids are used extensively in biological control programmes and, because of this, studies on cold storage started over 90 years ago. The ability to store reared biocontrol agents at low temperatures for certain duration provides an opportunity to accumulate or stockpile sufficient number of entomophagous insects for field release at proper weather conditions and make them available during high demand periods to the concerned farmers. Cold stored natural enemies can be synchronously released in the fields during critical stages of pest outbreaks. Cold storage also helps to keep viable stock of natural enemies when not needed and to minimize laboratory operations by prolonging their survival and delaying eclosion. Cold storage tolerance is highly plastic trait influenced by a range of biotic and abiotic factors experienced before, during and after cold exposure. These factors ultimately affect the development, longevity, fecundity, parasitization, sex-ratio and other fitness parameters along with morphology, behaviour and physiology of entomophagous insects. For the successful implementation of a cold storage project, knowledge of these diverse factors that cause storage and post-storage effects is essential. The potential of cold storage protocols in improving mass rearing and commercial production of bioagents is thus reviewed to access the strategies, innovations, techniques, devices and wisdom involved in the process of cold storage of entomophagous insects worldwide. 相似文献
6.
Singh BB Sharma JK Ghatak S Sharma R Bal MS Tuli A Gill JP 《Veterinary parasitology》2012,186(3-4):503-506
Echinococcosis is an important medical, veterinary and economic concern in India. Ten cysts were randomly selected from each intermediate host species (cattle, buffalo, sheep, goat and pigs). Either the germinal layer (sterile cysts) or protoscoleces (fertile cysts) were collected for molecular characterization. A 434 base pair fragment of the mitochondrial cytochrome oxidase-1 gene was amplified using PCR from each isolate. Ten representative samples (2 from each intermediate host species) were sequenced in both the directions from which readable sequences were obtained from nine for phylogenetic analysis (NCBI, Blast). Phylogenetic analysis of cytochrome oxidase I gene revealed that seven (77.7%) isolates, from cattle (2), pigs (2), buffaloes (1) and goat (2) were clustered with the Indian Buffalo (G3) strain of Echinococcus granulosus, while two (22.2%) isolates from sheep were clustered with the sheep strain (G1) of E. granulosus. Phylogenetic analysis of the cytochrome oxidase-1 gene revealed that the buffalo strain (G3) and common sheep strain (G1) are cycling among livestock in north India and that these strains are highly adapted to cattle, buffalo, sheep, goats and pigs. 相似文献
7.
In vitro and in vivo pathogenicity studies of Pasteurella multocida strains harbouring different ompA 总被引：1，自引：0，他引：1
Shailja Katoch Mandeep Sharma R. D. Patil Sandeep Kumar Subhash Verma 《Veterinary research communications》2014,38(3):183-191
Pasteurella multocida is a pathogenic, Gram-negative bacterium that is commonly found as normal flora in nasopharynx of variety of wild and domestic animals. Numerous virulence factors have been described for P. multocida isolates which include adherence and colonization factors, iron-regulated and acquisition proteins, extracellular enzymes such as neuraminidase, lipopolysaccharide (LPS), capsule and a variety of outer membrane proteins (Omp). OmpA has a significant role in stabilizing the cell envelope structure by providing physical linkage between the outer membrane & peptidoglycan. It has been shown to mediate P. multocida -host cells interaction via heparin and/or fibronectin binding and therefore act as an important invasive molecule which could determine the final outcome of initial infection. Comparative nucleotide sequence analysis of ompA gene of P. multocida has revealed that despite extensive genetic diversity in ompA of P. multocida, most sequences could be classified into two major allele classes namely ompA allele (I) and allele (II). The P. multocida recovered from nasal cavity of bovine and belonging to two ompA classes were tested for their differential virulence. In vitro pathogenicity studies on Madin Darby Bovine Kidney (MDBK) cell line employing adhesion and invasion assays indicated that P. multocida strain with ompA (I) is more invasive than P. multocida strain with ompA (II). In vivo studies in mice further reiterated that the isolates harbouring ompA(I) were comparatively more virulent to isolates harbouring ompA (II). 相似文献
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Investigation of an outbreak of sheeppox among native sheep breeds in the Western Himalayas of India
Chahota Rajesh Sharma Pardeep Kumar Rakesh Gupta Tania Sharma Mandeep 《Veterinary research communications》2022,46(1):101-107
Veterinary Research Communications - An outbreak of sheeppox was investigated in a cluster of villages situated in Western Himalayan ranges of a Northern Indian state. Non-migratory sheep... 相似文献
10.
Ravinder S. Chandel Sumit Vashisth Mandeep Pathania Pawan K. Mehta Abhishek Rana 《国际虫害防治杂志》2013,59(4):348-362
AbstractWhite grubs are highly polyphagous and most destructive soil pests inflicting damage to a wide variety of crops. In India, more than 1000 species of white grubs are known of which over 40 species attack wide range of plants. White grubs are naturally infected by various entomopathogens which include fungi, bacteria and nematodes. Entomopathogenic fungi offer great potential and members of genera Beauveria and Metarhizium are widely used against white grubs. Several commercial products of entomopathogenic fungi like Bio Green, ORY-X, Grub X 10G, Betel, Biotrol FMA and Meta-Guard have been developed for the control of white grubs. In India, good control of white grubs in paddy, ginger and sugarcane has been achieved with different entomofungi. Among EPNs, Heterorhabditis bacteriophora is moderately effective against Popillia japonica and Rhizotrogus majalis. H. indica and H. bacteriophora are effective against potato white grubs in India. Paenibacillus popilliae cause milky disease in P. japonica grubs. The bacterium is pathogenic to Holotrichia consanguinea, H. serrata and Leucopholis lepidophora. In north-western Himalaya, B. cereus is highly toxic to the grubs of H. seticollis and Anomala dimidiata. 相似文献