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Seshadri Shivashankar Manoharan Sumathi Vala Keshava Rao 《The Journal of Horticultural Science and Biotechnology》2017,92(2):113-119
Uneven ripening (UR) is a physiological disorder of unknown origin in ‘Bangalore blue’ grape (Vitis vinifera × Vitis labrusca) leading to wine of inferior quality. A preliminary study found wide variations in total dehydrogenase activity (TDH) of seeds from unevenly ripe berries. In our experiments, gibberellin (GA3) applied to young grapes increased seed TDH activity and reduced the incidence of uneven ripening to 2% compared with 35% in the control. In contrast, paclobutrazol (PBZ) decreased TDH activity and increased the incidence of the disorder to 58%. GA3-treated berries had higher concentrations of sucrose and TDH activity in seed representing mature seeds with high viability. In contrast, PBZ-treated and control berries had higher concentrations of glucose and lower TDH activity, indicating immature seeds with low viability. These results suggested that competition among developing berries can lead to differences in seed gibberellin content, seed viability and the rate of berry growth resulting in green, purple, and black berries at harvest. The study established the role of seed viability in uneven ripening and demonstrated that the incidence of the disorder is reduced by the application of GA3 to immature berries. 相似文献
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The use of a peroxidase labelled PCR generated probe followed by enhanced chemiluminescence hybridization assay detected infectious bursal disease virus directly from bursal imprints on a nylon membrane. Tissue imprint hybridization proved to be a simple, rapid and safe means of detecting IBD virus for screening large numbers of field samples. The PCR generated probe was highly specific for IBD virus and did not hybridize with cellular nucleic acids in control imprints. Tissue imprint hybridization was found to be a more sensitive method than conventional antigen detection assays. 相似文献
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Agroforestry Systems - Agroforestry is identified as a potential carbon sequestration strategy, involved in carbon capture and storage in woody perennials. However, the use of default values of... 相似文献
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Mak Chanratana Gwang Hyun Han Manoharan Melvin Joe Aritra Roy Choudhury Seshadri Sundaram Md. Abdul Halim 《Archives of Agronomy and Soil Science》2018,64(11):1489-1502
Methylobacterium oryzae CBMB20, a promising plant growth promoting bacteria (PGPB) and a biocontrol agent, was immobilized in different formulations such as wet chitosan, dry chitosan, wet alginate and dry alginate and were tested for tomato plant growth promotion. Chitosan solution (1.5%) with pH 5.5–6.0 and 90 min contact time was found optimal for immobilization. The chitosan formulations showed better entrapment efficiency and good degradability resistance apart from slow release of cells under prolonged incubation. Survivability of bacteria (80%) was observed in wet chitosan formulation even after 90 days of storage at 4°C. The spermosphere survival of bacteria was high in both dry and wet chitosan formulations applied soils even after 21 days under greenhouse conditions. While the alginate formulation degraded fully, partial degradation of chitosan formulation was observed even after 30 days, indicating its ability to support the survival of M. oryzae CBMB20 in soil. Plants inoculated with wet chitosan formulation registered 1.3 fold increase in the shoot and root length and dry weight compared to other treatments. Hence, chitosan formulation supporting better plant growth compared to alginate will be a better carrier for taking bacteria to the plant rhizosphere and thereby promote plant growth. 相似文献
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Manoharan S Sindhu G Nirmal MR Vetrichelvi V Balakrishnan S 《Pakistan journal of biological sciences: PJBS》2011,14(20):918-932
Investigation of expression pattern of molecular markers in oral epithelial tissues would help to assess the cell differentiation and proliferation as well as early diagnosis of precancerous and cancerous lesions of the oral cavity. Aim of the present study was to investigate the protective effect of berberine on expression pattern of apoptotic, cell proliferative, inflammatory and angiogenic markers during 7,12-dimethylbenz(a)anthracene (DMBA) induced hamster buccal pouch carcinogenesis. Immunohistochemical staining [p53, Bcl-2, Bax, Proliferating Cell Nuclear Antigen (PCNA) and Vascular Endothelial Growth Factor (VEGF)], Enzyme Linked Immuno Sorbent Assay (ELISA) [c-fos, COX-2, caspase-3 and -9] and Real-Time PCR [Cyclin D1 and NFkappaB] were utilized to assess the expression pattern of molecular markers in DMBA induced hamster buccal pouch carcinogenesis. Over expression of mutant p53, PCNA, Bcl-2 and VEGF were noticed in hamsters treated with DMBA alone. Decreased expression of Bax protein was noticed in hamsters treated with DMBA alone. Increased expression of C-fos, COX-2, NFkappaB and Cyclin D1 and decreased activities of caspase-3 and -9 were also noticed in hamsters treated with DMBA alone. Oral administration ofberberine at a dose of 75 mg kg(-1) b.w. brought back the expression of above mentioned molecular markers to near normal pattern in hamsters treated with DMBA. The present results thus suggest that berberine has potent anti-inflammatory, anti-angiogenic, anti-cell proliferative and apoptosis inducing properties in DMBA induced oral carcinogenesis. 相似文献
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Seshadri Shivashankar Manoharan Sumathi Hari Shankar Singh 《The Journal of Horticultural Science and Biotechnology》2016,91(2):138-147
Jelly seed (JS) in ‘Amrapali’ mango (Mangifera indica L.) is a physiological disorder, the cause of which has long remained obscure. The disorder is distinguished by the appearance of jelly-like tissue in the pulp adjoining the stone, although the fruit show no external symptoms. The objective of this study was to determine the causative factor inducing the JS disorder in ‘Amrapali’ mango. Studies showed, for the first time, that JS in ‘Amrapali’ mango arose at the start of germination-associated events in the seed of developing fruit. The trigger for premature seed germination originated from reduced synthesis of very-long-chain fatty acids (VLCFAs) in the seed of developing fruit. This then promoted the production of cytokinins, leading to the onset of premature germination-associated events in the seed. Consequently, a large increase in the activities of pectinolytic enzymes in JS pulp occurred that led to the rapid degradation of pectin and excessive softening of the pulp, to the consistency of jelly. The application of plant growth regulators to developing fruit showed that gibberellic acid (GA3) increased the incidence of JS, while paclobutrazol reduced the incidence of JS, confirming that the onset of early germination during fruit maturation and ripening played a primary role in the incidence of the JS disorder. 相似文献
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Kandasamy Rajamanickam Venkatasubramanian Leela Gopalakrishnan Suganya Sabiha Hayath Basha Manoharan Parthiban Natesan Pazhanivel Angappan Mangala Gowri 《Reproduction in domestic animals》2020,55(8):931-942
In swine, even though the pregnant sows were with iron abundance, the inborn iron reserve of piglets was compromised. This indicates the insufficiency of molecular machinery involved in local placental iron flux. Here, we investigated the expression of iron regulatory proteins like hepcidin and ferroportin and also their association with iron reserve, inflammation and oxidative stress in placenta of full-term pregnant sows (n = 6). Amplification and sequencing of placental DNA confirmed the presence of hepcidin (MN579557) and ferroportin (MN565887) sequences and their 100% identity with existing GenBank data. Real-time amplification of placental mRNA revealed significant higher expression of hepcidin (p < .05) than ferroportin. Western blot analysis of placental tissues revealed specific bands for both hepcidin (~8 kDa) and ferroportin (~62 kDa) molecules. Immunohistochemistry revealed the immunoreactivity for both proteins in the cytoplasm and membrane of trophoblastic cells of the placenta. Hepcidin and ferroportin expressions were positively associated with placental non-haem iron reserve (p < .0001; p = .033), lipid peroxidation (p = .0060; p < .0001) and reactive oxygen species level (p = .0092; p = .0292). Hepcidin expression was positively associated with interleukin – 6 (p = .0002) and interferon gamma (p < .0001) expressions but ferroportin expression was negatively associated with interleukin-6 (p = .0005), interleukin-1β (p = .0226) and interferon gamma (p = .0059) expressions. This indicates hepcidin and ferroportin may have a role in controlling the local placental iron flux by acting as a molecular bridge between iron trafficking and inflammation. 相似文献
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Becalski A Lau BP Lewis D Seaman SW Hayward S Sahagian M Ramesh M Leclerc Y 《Journal of agricultural and food chemistry》2004,52(12):3801-3806
The free amino acid profile and sugar (fructose, glucose, and sucrose) composition were determined in potato samples selected to give a large range of variation (a total of 66 samples). From these samples French fries were produced in a laboratory-scale simulation of an industrial process followed by a finish fry at 180 degrees C for 3.5 min using a restaurant fryer. The final product was blast frozen and analyzed for acrylamide. Acrylamide was detected in all samples, but its concentration varied significantly from 50 to 1800 ng/g. For isotope dilution (13C3) acrylamide analysis, samples were extracted with water, cleaned up on HLB Oasis polymeric and Accucat mixed mode anion and cation exchange SPE columns, and analyzed by LC-MS/MS. Statistical analysis of the data indicates that the effect of sugars and asparagine on the concentration of acrylamide in French fries is positive and significant (p < 0.001). It appears that one of the ways acrylamide formation in French fries can be effectively controlled is by the use of raw products with low sugar (and to a lesser degree, asparagine) content. 相似文献
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Periyasamy Rathinapriya Lakkakula Satish Subramani Pandian Ramakrishnan Rameshkumar Manoharan Balasangeetha Kasinathan Rakkammal 《Journal of plant nutrition》2020,43(19):2857-2875
Abstract In recent decades, chemical fertilizers create a thread to organic agriculture and food security. To overcome this issue, the present study aimed to investigate the influence of liquid seaweed extracts (LSEs) prepared from Padina boergesenii (PB) and Gracilaria edulis (GE) on growth, development, biochemical characteristics and yield traits of foxtail millet. The various concentrations of individual (20, 40, 60, and 80%; v/v) and combined (10?+?10, 20?+?20, 30?+?30, and 40?+?40%; v/v) PB and GE LSEs were supplied through foliar spray method. The results imply that germination bioassays, vegetative plant growth were significantly increased in lower concentration of LSEs. Further, exposure of foxtail plants to combined foliar spray application of (20?+?20%; v/v) LSEs enriched photosynthetic pigments, total soluble sugar and total soluble protein, compared with single LSEs and control foliar spray methods. Additionally, LSEs enhanced the yield attributes such as the average number of seeds (971.5/panicle) and mean length of panicle (18.4?cm) in PB?+?GE LSEs (20?+?20%; v/v) treatment. This is the first report, to assess the synergistic biostimulant ability of PB and GE LSEs in plant growth, quality improvement and yield attributes of foxtail millet. In conclusion, this present study suggests that combination of PB?+?GE LSEs foliar spray application could serve as an ideal biostimulant and a potential alternate to hazards chemical fertilizer in the green agriculture. 相似文献