The efficiency of nitrogen in cattle manures when applied to a double‐annual forage cropping system          下载免费PDF全文

B. Perramon  À. D. Bosch‐Serra  F. Domingo‐Olivé  J. Boixadera 《Grass and Forage Science》2017,72(4):676-690

The use of cattle manure (CM) for fertilization presents challenges for optimizing nitrogen (N) use. Our work aimed to assess N efficiencies, in a 6‐year experiment with three biennial rotations of four crops: oat–sorghum (first year) and ryegrass–maize (second year) in a rainfed humid Mediterranean area of Spain. Fertilization treatments included the following: control (no N), 250 kg mineral N ha^?1 year^?1 (250MN), three CM rates (supplying 170, 250 and 500 kg N ha^?1 year^?1) and four treatments where the two lowest CM rates were complemented with either 80 or 160 kg mineral N ha^?1 year^?1. Treatments were distributed randomly in each of three blocks. Maximum dry‐matter yield (~44–49 t ha^?1 rotation^?1) was achieved in the third rotation, and only the control and the 170CM yielded significantly less. Within the limitations of the EU Nitrate Directive, the N steady state supply of 170CM always requires a complement of mineral N (80 kg N ha^?1) to maximize N agronomic efficiency. The maximum N‐fertilizer replacement value (250CM vs. 250MN) was 0·67, without significant differences between the two treatments in other N‐related efficiency indexes, which indicates that plants took advantage of residual‐N effects. Nitrogen losses by leaching in the 250CM treatment were around 5–7% of the N applied. This reinforces the sustainability of manure recycling in long cropping seasons.  相似文献   

Pathologic and immunohistochemical findings in an outbreak of systemic toxoplasmosis in a mob of red kangaroos     

Mariano Carossino  Rudy Bauer  Mark A. Mitchell  Charles O. Cummings  Anke C. Sthr  Nobuko Wakamatsu  Kimberly Harper  Ingeborg M. Langohr  Kendra Schultz  Maria S. Mitchell  Daniel K. Howe  Udeni B. R. Balasuriya 《Journal of veterinary diagnostic investigation》2021,33(3):554

Toxoplasma gondii is a zoonotic protozoan pathogen that infects many endothermic vertebrates, including humans; the domestic cat and other felids serve as the definitive host. Macropodids are considered highly susceptible to toxoplasmosis. Here, we describe the clinical, pathologic, and immunohistochemical findings of an outbreak of systemic toxoplasmosis in a mob of 11 red kangaroos (Macropus rufus), with high morbidity (73%) and mortality (100%) rates. Affected animals had either severe and rapidly deteriorating clinical conditions or sudden death, which was correlated with widespread necrotizing lesions in multiple organs and intralesional T. gondii organisms identified via MIC3-specific immunohistochemistry and confirmed by REP529-specific rtPCR. Quantification of parasites demonstrated the highest parasite density in pulmonary parenchyma compared with other tissues. Our study highlights the continued importance of this severe condition in Australian marsupials.  相似文献   

Immunohistochemical characterisation of PCV2 associate lesions in lymphoid and non-lymphoid tissues of pigs with natural postweaning multisystemic wasting syndrome (PMWS)   总被引：9，自引：0，他引：9  

Chianini F  Majó N  Segalés J  Domínguez J  Domingo M 《Veterinary immunology and immunopathology》2003,94(1-2):63-75

The lymphoid, renal, pulmonary, and hepatic lesions of naturally occurring postweaning multisystemic wasting syndrome (PMWS) affected pigs have been studied by means of immunohistology. Ten conventionally reared pigs showing acute clinical signs of PMWS were selected from a farm on which animal were seronegative to porcine reproductive and respiratory virus and to Aujeszky's disease virus. All pigs were positive in tests for porcine circovirus type 2 by ISH and IHC. Monoclonal and polyclonal antibodies to CD3, CD79alpha, CD45RA (3C3/9), lysozyme, SLA-II-DQ (BL2H5), and MAC387 were used to characterise cells in PMWS lesions. The most relevant changes were reduction or loss of B and T lymphocytes, increased numbers of macrophages, and partial loss and redistribution of antigen presenting cells throughout lymphoid tissues compared to uninfected controls. The characteristics of lymphoid lesions in the present study strongly suggest an immunosuppressive effect of PMWS in affected pigs.  相似文献   

Dynamics of porcine circovirus type 2 infection in a herd of pigs with postweaning multisystemic wasting syndrome   总被引：6，自引：0，他引：6  

Rodríguez-Arrioja GM  Segalés J  Calsamiglia M  Resendes AR  Balasch M  Plana-Duran J  Casal J  Domingo M 《American journal of veterinary research》2002,63(3):354-357

OBJECTIVE: To determine the pattern of infection for porcine circovirus type 2 (PCV2) in a herd of pigs with postweaning multisystemic wasting syndrome (PMWS). ANIMALS: 29 sows and 250 pigs. PROCEDURE: Blood samples were collected from all 3-, 7-, and 12-week old pigs and 59 pigs at 28 weeks of age. Pigs that died during the study were necropsied. Porcine parvovirus and PCV2 antibodies were assayed. A polymerase chain reaction (PCR) was used to detect PCV2 genome in serum of selected pigs. RESULTS: The PMWS started when pigs were 8 weeks old, with a prevalence of 30% in 8- to 10-week-old pigs. Eighty-three pigs died during the period between 3 and 12 weeks of age. Microscopic lesions consistent with PMWS were observed, and PCV2 nucleic acid was detected (50 of 68 pigs). Antibodies to PCV2 decreased from 3 to 7 weeks of age, increased at 12 weeks of age, and were maintained until 28 weeks of age. One sow had a positive result for PCR of serum. Nine, 37 and 8 pigs had PCV2 genome in serum obtained at 7, 12, and 28 weeks of age, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Infection with PCV2 coincided with severe clinical signs; however, infected 28-week-old pigs did not have evidence of disease. Immunity declined over time in young pigs. A long duration of PCV2 viremia was apparent in a high percentage of infected pigs, which may affect transmission and persistence of the virus in a herd.  相似文献   

Foot-and-mouth disease virus   总被引：11，自引：0，他引：11  

Domingo E  Baranowski E  Escarmís C  Sobrino F 《Comparative immunology, microbiology and infectious diseases》2002,25(5-6):297-308

Foot-and-mouth disease virus (FMDV) is an aphthovirus of the family Picornaviridae and the etiological agent of the economically most important animal disease. As a typical picornavirus, FMD virions are nonenveloped particles of icosahedral symmetry and its genome is a single stranded RNA of about 8500 nucleotides and of positive polarity. FMDV RNA is infectious and it replicates via a complementary, minus strand RNA. FMDV RNA replication is error-prone so that viral populations consist of mutant spectra (quasispecies) rather than a defined genomic sequence. Therefore FMDV in nature is genetically and antigenically diverse. This poses important challenges for the diagnosis, prevention and control of FMD. A deeper understanding of FMDV population complexity and evolution has suggested requirements for a new generation of anti-FMD vaccines. This is relevant to the current debate on the adequacy of non-vaccination versus vaccination policies for the control of FMD.

Résumé

Le virus de la fièvre aphteuse est un aphtovirus de la famille des Picornaviridae et l'agent de la maladie animale la plus importante sur le plan économique. En tant que picornavirus typique, le virus de la fièvre aphteuse est nu, sous forme d'icosaèdre et son génome comprend un acide ribonucléique monobrin avec environ 8500 nucléotides et une polarité positive. L'acide ribonucléique de ce virus est infectieux et il se réplique par l'intermédiaire d'un brin d'ARN moins, complémentaire. La réplication de l'acide nucléique de ce virus conduit à des erreurs, de telle sorte que les populations virales comprennent un ensemble de mutants (quasi espèce) plutôt qu'une séquence génomique bien définie. Par suite, le virus de la fièvre aphteuse est génétiquement et antigéniquement varié. Ceci entraîne des difficultés importantes pour le diagnostic, la prévention et la maîtrise de la fièvre aphteuse. Une connaissance plus approfondie de la complexité et de l'évolution de la population de ce virus a conduit à des besoins pour une nouvelle génération de vaccines aphteux. Ceci est lié au débat actuel sur le choix d'une politique de vaccination ou de non-vaccination dans la lutte contre la fièvre aphteuse.  相似文献   

Mycobacterium infection in a captive-reared capercaillie (Tetrao urogallus)     

Marco I  Domingo M  Lavin S 《Avian diseases》2000,44(1):227-230

One captive male capercaillie (Tetrao urogallus) was found dead on December 1993 at the breeding center of capercaillie in Catalonia, Spain. The bird was emaciated and, at necropsy, had numerous nodules of various sizes subcutaneously in the cervical region, pleura, lungs, liver, spleen, and mesentery. Microscopic examination revealed granulomatous lesions with central caseous necrosis, epithelioid cells, giant cells, and few lymphocytes in all affected organs. Numerous acid-fast bacilli were demonstrated in the tubercles with Ziehl-Nielsen stain.  相似文献   

Response of apricot trees to deficit irrigation strategies     

A. Pérez-Pastor  R. Domingo  A. Torrecillas  Ma. C. Ruiz-Sánchez 《Irrigation Science》2009,27(3):231-242

During four growing seasons, 10-year-old apricot trees (Prunus armeniaca L., cv. ‘Búlida’) were submitted to three different drip irrigation regimes: (1) a control treatment, irrigated at 100% of seasonal crop evapotranspiration (ETc), (2) a continuous deficit irrigation (DI) treatment, irrigated at 50% of the control treatment, and (3) a regulated deficit irrigation (RDI) treatment, irrigated at 100% of ETc during the critical periods, which correspond to stage III of fruit growth and 2 months after harvest (early postharvest), and at 25% of ETc during the rest of the non-critical periods in the first two growing seasons and at 40% of ETc in the third and fourth. Soil–plant–water relation parameters were sensitive to the water deficits applied, which caused reductions in leaf and soil water potentials. The longer and severer deficits of the RDI treatment decreased fruit yield in the first two seasons. The RDI treatment pointed to two threshold values that defined the level at which both plant growth and yield were negatively affected with respect to the control treatment: (1) a predawn leaf water potential of around −0.5 MPa during the critical periods, and (2) a 22% drop in irrigation water. The total yield obtained in the DI treatment was significantly reduced in all the years studied due to the lower number of fruits per tree. No changes in the physical characteristics of fruits were observed at harvest. RDI can be considered a useful strategy in semiarid areas with limited water resources.  相似文献   

How much variation can be explained by seasonal,spatial and environmental effects in nekton assemblages of the Terminos Lagoon?     

Julia Ramos Miranda  David Mouillot  Atahualpa Sosa Lopez  Thang Do Chi  Domingo Flores Hernández 《水产资源保护:海洋与淡水生态系统》2008,18(5):508-517

• 1. The coastline is very sensitive to both climatic and human influences but also provides essential goods and services upon which human welfare depends. Species inhabiting these coastal areas provide valuable resources and enhance ecosystem functioning: it is therefore essential to understand the main factors influencing species abundance patterns. The aim of this study is to estimate the amount of variation in nekton coastal assemblages explained by spatial, temporal and environmental variables.
• 2. Nekton sampling was carried out over 17 months in the Terminos Lagoon, a reserve area situated in the Gulf of Mexico, using a shrimp trawl. At each station, 11 environmental variables were recorded. The influence of seasonal, spatial and environmental variables on nekton species abundance was analysed using Partial Canonical Correspondence Analysis (PCCA).
• 3. The explanatory variables explained only 19.7% of the total observed variation in the nekton community for the Terminos Lagoon. The major part of the explained variation was due to the spatial effect (9.2%), while environmental variables and seasonality explained only 3.6% and 0.8%, respectively, suggesting the necessity to consider experimental fishing design in such artisanal fisheries.
• 4. Results also suggest focusing on more integrative indicators of community structure such as measures of taxonomic diversity based on presence/absence or on measures related to functional attributes of communities rather than using species abundance patterns to predict changes in coastal ecosystems.

Copyright © 2007 John Wiley & Sons, Ltd.  相似文献   

Molecular evidence of Leishmania spp. in spider monkeys (Ateles geoffroyi) from The Tuxtlas Biosphere Reserve,Veracruz, Mexico     

Pérez-Brígido  Carlos D.  Romero-Salas  Dora  Pardío-Sedas  Violeta T.  Cruz-Romero  Anabel  González-Hernández  Milagros  Delprá-Cachulo  Joyce Mara  Ascencio  Mariano  Florin-Christensen  Mónica  Schnittger  Leonhard  Rodríguez  Anabel E. 《Veterinary research communications》2022,46(1):295-302

Veterinary Research Communications - The black-handed spider monkey (Ateles geoffroyi) is a platyrrhine primate distributed in southern Mexico, Central America, and part of South America. Two...  相似文献   

Radial variation in sap flow in five laurel forest tree species in Tenerife,Canary Islands   总被引：1，自引：0，他引：1  

Jiménez MS  Nadezhdina N  Cermák J  Morales D 《Tree physiology》2000,20(17):1149-1156

Variations in radial patterns of xylem water content and sap flow rate were measured in five laurel forest tree species (Laurus azorica (Seub.) Franco, Persea indica (L.) Spreng., Myrica faya Ait., Erica arborea L. and Ilex perado Ait. ssp. platyphylla (Webb & Berth.) Tutin) growing in an experimental plot at Agua García, Tenerife, Canary Islands. Measurements were performed around midday during warm and sunny days by the heat field deformation method. In all species, water content was almost constant (around 35% by volume) over the whole xylem cross-sectional area. There were no differences in wood color over the whole cross-sectional area of the stem in most species with the exception of E. arborea, whose wood became darker in the inner layers. Radial patterns of sap flow were highly variable and did not show clear relationships with tree diameter or species. Sap flow occurred over the whole xylem cross-sectional area in some species, whereas it was limited to the outer xylem layers in others. Sap flow rate was either similar along the xylem radius or exhibited a peak in the outer part of the xylem area. Low sap flow rates with little variation in radial pattern were typical for shaded suppressed trees, whereas dominant trees exhibited high sap flow rates with a peak in the radial pattern. Stem damage resulted in a significant decrease in sap flow rate in the outer xylem layers. The outer xylem is more important for whole tree water supply than the inner xylem because of its larger size. We conclude that measurement of radial flow pattern provides a reliable method of integrating sap flow from individual measuring points to the whole tree.  相似文献