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1.
Haematological and biochemical investigations were performed on 14 koalas with uncomplicated cystitis, 8 with complicated cystitis, 8 with conjunctivitis, 8 with lymphosarcoma, and 14 with miscellaneous diseases. Changes were limited and inconsistent in individual koalas with uncomplicated cystitis and conjunctivitis. In contrast, individual koalas with complicated cystitis were more likely to have anaemia, leukocytosis due to neutrophilia, hypoproteinaemia due to hypoalbuminaemia, and azotaemia due to elevated urea concentration. Although these changes were non-specific they did allow assessment of prognosis for survival and response to treatment. Koalas with lymphosarcoma were invariably anaemic, leukaemic, azotaemic and hypoalbuminaemic. Elevated enzymes (aspartate transaminase [AST]. lactate dehydrogenase [LD] and gamma glutamyl transferase [GGT]) were more common in koalas with lymphosarcoma. Koalas affected by miscellaneous conditions showed variable changes but once again anaemia, leukocytosis, azotaemia, elevated AST and LD, and hypoalbuminaemia were not uncommon. On the basis of these findings a minimal profile is suggested for the investigation of sick koalas and would include haematocrit, total and differential leukocyte counts, urea, total protein and albumin concentrations and AST, GGT and LD activities. 相似文献
2.
de Siqueira A Cassiano FC Landi MF Marlet EF Maiorka PC 《Journal of Feline Medicine and Surgery》2012,14(10):723-728
Animal cruelty is defined as a deliberate action that causes pain and suffering to an animal. In Brazil, legislation known as the Environmental Crimes Law states that cruelty toward all animal species is criminal in nature. From 644 domestic cats necropsied between January 1998 and December 2009, 191 (29.66%) presented lesions highly suggestive of animal cruelty. The main necroscopic finding was exogenous carbamate poisoning (75.39%) followed by blunt-force trauma (21.99%). Cats from 7 months to 2 years of age were the most affected (50.79%). In Brazil, violence is a public health problem and there is a high prevalence of domestic violence. Therefore, even if laws provide for animal welfare and protection, animals are common targets for violent acts. Within a context of social violence, cruelty toward animals is an important parameter to be considered, and the non-accidental lesions that were found are evidence of malicious actions. 相似文献
3.
CA Souza NM Ocarino JF Silva JN Boeloni EF Nascimento IJ Silva RD Castro LP Moreira FRCL Almeida H Chiarini‐Garcia R Serakides 《Reproduction in domestic animals》2011,46(1):e7-e16
The aim of this study was to evaluate the effects of thyroxine administration on morphometric parameters, expression of vascular endothelial growth factor (VEGF) and vascularization in the uterus and placenta and reproductive parameters in gilts at 70 days of gestation. At 150 days of age, i.e., before first heat, 20 gilts were randomly divided into two experimental groups: treated (n = 10) and control (n = 10). The treated group received a daily dose of 400 μg of l ‐thyroxine (T4) in their diet until slaughter and the control group received only typical meals. Before artificial insemination, blood was collected to determine plasma total T4. The gilts were inseminated in the second oestrus and slaughtered at 70 days of gestation. The foetal thyroid follicular epithelium height, number, size and weight of foetuses; foetal myogenesis, corpora lutea number, embryonic mortality rate, uterine weight, placental weight and placental fluid volume were measured. Histomorphometric and immunohistochemical analysis of uterus and placenta were determined. The averages of all variables were compared by the Student’s t‐test. The gilts treated with thyroxine showed significant increase of plasma total T4. At 70 days of gestation, the heights of the trophoblastic epithelium, endometrial epithelium and endometrial gland epithelium were significantly higher in the group treated with T4. The expression of cytoplasmatic and nuclear VEGF in trophoblastic cells and the number of blood vessels per field in endometrial stroma were significantly higher in the gilts treated with T4. No other significant differences between groups were obtained with respect to other parameters (p > 0.05). We conclude that oral administration of T4 up to 70 days of pregnancy in gilts affects the morphometric parameters, the expression of placental VEGF and the uterine vascularization but does not affect reproductive parameters in gilts during early gestation. 相似文献
4.
MR N'Diaye SS Sun SP Fanua KJ Loseth EF Shaw Wilgis BG Crabo 《Reproduction in domestic animals》2002,37(5):305-309
Supplementation of exogenous growth hormone (GH) during prepuberty advances onset of spermatogenesis in boars, but the mechanism of action is unknown. The present study is an investigation of the presence and characteristics of testicular growth hormone receptors (GHR). A total of 36 boars were castrated, three boars every 10 days, between the ages of 10 and 120 days. Testicular membrane preparations of 10, 20, 30, 50, 70, 100 and 120‐day‐old boars were used to determine 125I‐bGH binding and Scatchard analysis. Liver from a 60‐kg barrow was used for comparison. Specific 125I‐bGH binding to testicular membrane preparations occurred in all age groups with the exception of 20‐day‐old boars at levels of 30–40% of liver binding. At 30 days of age the unlabelled bGH at 1.1 ng/tube achieved half maximal inhibition (ID50). Results of Scatchard analysis indicated a single class of binding sites. Binding affinity was 2.89 × 109m with a binding capacity of 12 fmole/mg membrane protein. The results from this study suggest that GH may act directly on the cells of the prepubertal boar testis. 相似文献
5.
JF Silva NM Ocarino ALS Vieira EF Nascimento R Serakides 《Reproduction in domestic animals》2013,48(4):691-698
Although thyroid dysfunction occurs frequently in humans and some animal species, the mechanisms by which hypo‐ and hyperthyroidism affect the corpus luteum have not been thoroughly elucidated. This study evaluated the levels of proliferative activity, angiogenesis, apoptosis and expression of cyclooxygenase‐2 in the corpus luteum of female rats with thyroid dysfunction. These processes may be important in understanding the reproductive changes caused by thyroid dysfunction. A total of 18 adult female rats were divided into three groups (control, hypothyroid and hyperthyroid) with six animals per group. Three months after treatment to induce thyroid dysfunction, the rats were euthanized in the dioestrus phase. The ovaries were collected and immunohistochemically analysed for expression of the cell proliferation marker CDC‐47, vascular endothelial growth factor (VEGF), VEGF receptor Flk‐1 and cyclooxygenase‐2 (COX‐2). Apoptosis was evaluated using the TUNEL assay. Hypothyroidism reduced the intensity and area of COX‐2 expression in the corpus luteum (p < 0.05), while hyperthyroidism did not alter COX‐2 expression in the dioestrus phase. Hypothyroidism significantly reduced the expression of CDC‐47 in endothelial cells and pericytes in the corpus luteum, whereas hyperthyroidism did not induce a detectable change in CDC‐47 expression (p > 0.05). Hypothyroidism reduced the level of apoptosis in luteal cells (p < 0.05) and increased VEGF expression in the corpus luteum. In contrast, hyperthyroidism increased the level of apoptosis in the corpus luteum (p < 0.05). In conclusion, thyroid dysfunction differentially affects the levels of proliferative activity, angiogenesis and apoptosis and COX‐2 expression in the corpus luteum of female rats. 相似文献
6.
FJF Sant’Ana EF Nascimento PF Andrés Laube EJ Gimeno CG Barbeito 《Reproduction in domestic animals》2009,44(6):889-893
The lectin‐binding pattern was compared in the normal and pathological uterus of sows during the ovarian cycle. The following biotinylated lectins were used: Con A, DBA, SBA, PNA, RCA‐I, UEA‐I and WGA. Glycoconjugate labelling showed differences between phases of ovarian cycle and presence of morphologic lesions. Cystic endometrial hyperplasia increased the RCA‐I reaction in the apical region of the glandular epithelium. There was higher intensity of labelling of WGA in the glandular epithelium in uteri with endometritis. In addition, increased Con A binding in the glandular epithelium and mild reduction of UEA‐I reactivity in the glycocalyx of the glandular epithelium were detected in the cases of endometritis. The results of this study show that morphologic alterations modify the sugar pattern in the porcine uterus. These modifications in glycoconjugates may be one of the reasons for decreased fertility in sows. 相似文献
7.
Haematological and biochemical reference values were established from 45 clinically healthy koalas. Statistical analysis revealed no significant differences for sex and season of sampling. Immature koalas had significantly higher alkaline phosphatase and inorganic phosphate values, and significantly lower total protein concentrations due to low globulins values. Enzyme reference values tended to be wide and could limit their usefulness in detecting disease. In the reference values for leukocytes, neutrophils and lymphocytes, the inclusion of low values which were not actually seen may interfere with the detection of reduced levels due to disease. 相似文献
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9.
N. Condom M. Kuper S. Marlet V. Valles J. Kijne 《Land Degradation \u0026amp; Development》1999,10(2):123-140
Evidence of soil degradation impeding soil tillage and irrigation in cultivated soils in Pakistan is identified, described and represented in a general process of degradation. Based on a chemical analysis of soil characteristics, it is shown that a more general geochemical degradation process may occur in these soils. Two paths of salinization, i.e. neutral salinization and alkalinization inducing a process of sodification, are identified. The wide range of chemical properties of soils and corresponding geochemical processes can be explained by the great diversity of quality in irrigation water that is taken either from the canal or from the groundwater. The basic module of a geochemical model AQUA (Vallès and DeCockborne, 1992) is calibrated with the help of a study of the soil geochemical properties (identification of minerals, characterization of exchanges) and then used to assess the effect of four different water qualities on sandy and loamy soils. Based on these scenarios, the salinity, alkalinity and sodicity hazard of irrigation water is assessed by taking into account simultaneously the electrical conductivity and the residual alkalinity (calcite-residual alkalinity, residual sodium carbonates) or irrigation water and the soil cation exchange capacity: these three indicators appear the most relevant in the context of the study. Copyright © 1999 John Wiley & Sons, Ltd. 相似文献
10.
Salt balance methods are generally applied in the root-zone and at local scales but do not provide relevant information for salinity management at irrigation scheme scales, where there are methodological impediments. A simple salt balance model was developed at irrigation scheme and yearly time scales and applied in Fatnassa oasis (Nefzaoua, Tunisia). It accounts for input by irrigation, export by drainage and groundwater flow, and provides novel computation of the influence of biogeochemical processes and variations in the resident amount of salt for each chemical component in the soil and shallow groundwater. Impediments were overcome by limiting the depth of the system so that the resident amount of salt that remained was of the same order of magnitude as salt inputs and allowed indirect and reliable estimation of groundwater flow. Sensitivity analyses as partial derivatives of groundwater salinity were carried out according to non-reactive salt balance under steady-state assumption. These analyses enabled the magnitude of the salinization process to be foreseen as a function of hydrological changes linked to irrigation, drainage, groundwater flow and extension of the irrigated area. From a salt input of 39 Mg ha−1 year−1 by irrigation, 21 Mg ha−1 year−1 (54%) and 10 Mg ha−1 year−1 (26%) were exported by groundwater flow and drainage, respectively. 7 Mg ha−1 year−1 (18%) were removed from groundwater by geochemical processes, while a non-significant 2 Mg ha−1 year−1 were estimated to have been stored in the soil and shallow groundwater where the residence time was only 2.7 years. The leaching efficiency of drainage was estimated at 0.77. With a water supply of 1360 mm by irrigation and 90 mm by rainfall, drainage, groundwater flow and actual evapotranspiration were 130, 230, and 1090 mm, respectively. The current extension of date palm plantations and salinization of groundwater resources are expected to significantly increase the salinity hazard while the degradation of the drainage system is expected to be of lesser impact. The approach was successfully implemented in Fatnassa oasis and proved to be particularly relevant in small or medium irrigation schemes where groundwater fluxes are significant. 相似文献