Antagonism of medetomidine sedation by atipamezole in pigs.   总被引：1，自引：0，他引：1  

R Nishimura  H Kim  S Matsunaga  M Sakaguchi  N Sasaki  H Tamura  A Takeuchi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1992,54(6):1237-1240

The efficacy of atipamezole as a medetomidine antagonist was evaluated in pigs. The atipamezole doses (intramuscularly) were 80, 160, 320 and 480 micrograms/kg of body weight, which were one, two, four and six times higher than the preceding medetomidine dose (80 micrograms/kg, intramuscularly). Atipamezole effectively reversed medetomidine-induced sedation, and the optimal action was seen at doses of 160 and 320 micrograms/kg. Recovery from sedation was quick and smooth, and adverse effects such as hyperactivity or tachycardia were minimal with either dose.  相似文献   

Pharmacokinetics and effects on clinical and physiological parameters following a single bolus dose of propofol in common marmosets (Callithrix jacchus)     

Kanako Muta  Takako Miyabe‐Nishiwaki  Kenichi Masui  Isao Yajima  Tomoya Iizuka  Akihisa Kaneko  Ryohei Nishimura 《Journal of veterinary pharmacology and therapeutics》2021,44(1):18-27

The objectives of this study were (a) to establish a population pharmacokinetic model and (b) to investigate the clinical and physiological effects of a single bolus dose of propofol in common marmosets. In Study 1, pharmacokinetic analysis was performed in six marmosets under sevoflurane anaesthesia. 8 mg/kg of propofol was administrated at a rate of 4 mg kg^?1 min^?1. Blood samples were collected 2, 5, 15, 30, 60, 90, 120 or 180 min after starting propofol administration. Plasma concentration was measured, and population pharmacokinetic modelling was performed. A two‐compartment model was selected as the final model. The population pharmacokinetic parameters were as follows: V₁ = 1.14 L, V₂ = 77.6 L, CL₁ = 0.00182 L/min, CL₂ = 0.0461 L/min. In Study 2, clinical and physiological parameters were assessed and recorded every 2 min after 12 mg/kg of propofol was administrated at a rate of 4 mg kg^?1 min^?1. Immobilization was sustained for 5 min following propofol administration without apparent bradycardia. While combination of propofol and sevoflurane caused apnoea in Study 1, apnoea was not observed following single administration of propofol in Study 2. These data provide bases for further investigation on intravenous anaesthesia using propofol in common marmosets.  相似文献   

Effect of dipeptide on intestinal peptide transporter 1 gene expression: An evaluation using primary cultured chicken intestinal epithelial cells     

Yukako Tokutake  Marcin Taciak  Kan Sato  Masaaki Toyomizu  Motoi Kikusato 《Animal Science Journal》2021,92(1):e13604

Peptide transporter 1 (PepT1) is a transporter responsible for absorbing dipeptide and tripeptide in enterocytes and is upregulated by dipeptide in mammals. It has not been certain whether intestinal PepT1 expression is responsive to dipeptides in chickens because of the lack of in vitro study using the cultured enterocytes. This study established a primary culture model of chicken intestinal epithelial cells (IECs) in two-dimensional monolayer culture using collagen gel by which the response of chicken PepT1 gene expression to dipeptide stimuli was evaluated. The cultured chicken IECs showed the epithelial-like morphology attached in a patch-manner and exhibited positive expression of cytokeratin and epithelial cadherin, specific marker proteins of epithelial cells. Moreover, the chicken IECs exhibited the gene expression of intestinal cell type-specific marker, villin1, mucin 2, and chromogranin A, suggesting that the cultured IECs were composed of enterocytes as well as goblet and enteroendocrine cells. PepT1 gene expression was significantly upregulated by synthetic dipeptide, glycyl-l-glutamine, in the cultured IECs. From the results, we herein suggested that dipeptide is a factor upregulating PepT1 gene expression in chicken IECs.  相似文献   

Augmented Repair of an Achilles Tendon Rupture Using the Flexor Digitorum Lateralis Tendon in a Toy Poodle          下载免费PDF全文

Masaaki Katayama 《Veterinary surgery : VS》2016,45(8):1083-1086

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Molecular cloning of a cDNA encoding the feline CD62L     

Nishimura Y  Shimojima M  Tohya Y  Miyazawa T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(1):81-84

We cloned a cDNA fragment encoding a feline homologue of L-selectin (CD62L). The extracellular region of the feline CD62L fragment contained a calcium-dependent (C-type) lectin domain, an epidermal growth factor-like domain, and two Sushi/CCP/SCR domains. The flow cytometric analysis confirmed that the feline CD62L molecule, which was expressed 293T cells, retained an epitope recognized by an anti-human CD62L monoclonal antibody (Leu-8).  相似文献   

Trade-off evaluation using carbon dioxide equivalent and hazard index of a paddy soil with application of organic liquid fertilizer     

Shohei RIYA  Ryo IMANO  Jining LI  Huifeng SUN  Sheng ZHOU  Masaaki HOSOMI 《土壤圈》2022,32(6):928-932

<正>Dear Editor,Rice is an important staple food crop in Asia, but the anaerobic soil conditions in rice fields cause emission of the potent greenhouse gas, methane(CH₄)(Le Mer and Roger,2001), and promote the accumulation of arsenic(As) in the grain(Le Mer and Roger, 2001; Khanam et al., 2020).To facilitate sustainable agriculture, using wastewater as an organic fertilizer is essential for reducing wastewater treatment-associated energy consumption and the recycling of wastewate...  相似文献   

A plant vacuolar protease, VPE, mediates virus-induced hypersensitive cell death   总被引：1，自引：0，他引：1  

Hatsugai N  Kuroyanagi M  Yamada K  Meshi T  Tsuda S  Kondo M  Nishimura M  Hara-Nishimura I 《Science (New York, N.Y.)》2004,305(5685):855-858

Programmed cell death (PCD) in animals depends on caspase protease activity. Plants also exhibit PCD, for example as a response to pathogens, although a plant caspase remains elusive. Here we show that vacuolar processing enzyme (VPE) is a protease essential for a virus-induced hypersensitive response that involves PCD. VPE deficiency prevented virus-induced hypersensitive cell death in tobacco plants. VPE is structurally unrelated to caspases, although VPE has a caspase-1 activity. Thus, plants have evolved a regulated cellular suicide strategy that, unlike PCD of animals, is mediated by VPE and the cellular vacuole.  相似文献   

Hepatic n-3/n-6 polyunsaturated fatty acid shift improves hepatic steatosis in farnesoid X receptor-null mice     

Masaaki Miyata  Yuichi Kinoshita  Kouhei Shinno  Yoshimasa Sugiura  Kazuki Harada 《Fisheries Science》2016,82(3):529-536

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Quantitative structure-activity analysis of larvicidal 1-(substituted benzoyl)-2-benzoyl-1-tert-butylhydrazines against Chilo suppressalis     

Nobuhiro Oikawa  Yoshiaki Nakagawa  Keiichiro Nishimura  Tamio Ueno  Toshio Fujita 《Pest management science》1994,41(2):139-147

The larvicidal activity of a number of 1-(substituted benzoyl)-2-benzoyl–1 -ten-butylhydrazines against the rice stem borer (Chilo suppressalis Walk.) was measured. Variations in the activity were examined quantitatively using physico-chemical substituent and molecular parameters and regression analysis. The results indicated that the molecular hydrophobicity and the electron-withdrawing inductive/ field effect of ontho substituents are favourable to larvicidal activity. The bulkiness of substituents at the meta and para positions was unfavourable to activity, substitution at the para position being more unfavourable than that at the meta position in terms of van der Waals' volume. The 2,3–, 2,5- and 2,6-disubstitution patterns were also unfavourable to activity. Reductions in larvicidal activity caused by the 2,6-,- 2,3,5- and 2,3,4,5-substitutions were greater than those induced by the 2,3- and 2,5-disubstitutions. When the sum of contributions from favourable effects is greater than that from unfavourable effects, the larvicidal activity is expected to be superior to that of the unsubstituted compound.  相似文献   

Telomere length and telomerase activity in canine mammary gland tumors     

Yazawa M  Okuda M  Setoguchi A  Iwabuchi S  Nishimura R  Sasaki N  Masuda K  Ohno K  Tsujimoto H 《American journal of veterinary research》2001,62(10):1539-1543

OBJECTIVE: To measure telomere length and telomerase activity in naturally occurring canine mammary gland tumors. SAMPLE POPULATION: 27 mammary gland tumor specimens obtained during resection or necropsy and 12 mammary gland tissue specimens obtained from healthy (control) dogs. PROCEDURE: Telomere length in tissue specimens was measured by use of restriction endonuclease digestion and Southern blot analysis. Telomerase activity was measured by use of a telomeric repeat amplification protocol assay. RESULTS: Telomere length in mammary gland tumors ranged from 11.0 to 21.6 kilobase pairs (kbp; mean +/- SEM, 14.5+/-0.5 kbp) but did not differ among tumor types. Telomeres in mammary gland tumors were slightly shorter than in normal tissue specimens, but telomere length could not be directly compared between groups, because mean age of dogs was significantly different between groups. Age was negatively correlated with telomere length in control dogs but was not significantly correlated with length in affected dogs. Telomerase activity was detected in 26 of 27 mammary gland tumors and in 4 of 12 normal tissue specimens. However, telomerase activity and telomere length were not correlated in tumor specimens. CONCLUSIONS AND CLINICAL RELEVANCE: Telomere length is maintained in canine mammary gland tumors regardless of the age of the affected dog. Measurement of telomere length may be a useful tool for monitoring the in vivo effects of telomerase inhibitors in dogs with tumors.  相似文献