The possibility of using oxidation–reduction potential to evaluate fish freshness     

Tri Winarni Agustini  Masaki Suzuki  Toru Suzuki  Tomoaki Hagiwara  Shoichi Okouchi  Rikuo Takai 《Fisheries Science》2001,67(3):547-549

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Minimal Set of Metabolic Pathways Suggested from the Genome of Onion Yellows Phytoplasma   总被引：1，自引：0，他引：1  

Kenro OSHIMA  Shin-ichi MIYATA  Toshimi SAWAYANAGI  Shigeyuki KAKIZAWA  Hisashi NISHIGAWA  Hee-Young JUNG  Ken-ichiro FURUKI  Masaki YANAZAKI  Shiho SUZUKI  Wei WEI  Tsutomu KUBOYAMA  Masashi UGAKI  Shigetou NAMBA 《Journal of General Plant Pathology》2002,68(3):225-236

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A modified fluorometric method to quantify the concentration effect (pI₅₀) of photosystem II-inhibiting herbicides     

Masaki Hiraki  Wim J. Vredenberg  Jack J.S. van Rensen  Ko Wakabayashi 《Pesticide biochemistry and physiology》2004,80(3):183-191

Chlorophyll fluorescence induction curves of isolated thylakoids were measured in the absence and in the presence of various concentrations of photosystem II-inhibiting herbicides. A mathematical program was applied to simulate the curves. Based on these simulated curves a new method is developed to determine the concentration effect (pI₅₀) of the herbicides. The results of the new method correspond well with reported values in the literature. The method is very convenient and time-saving.  相似文献   

Role of the hyaluronan receptor CD44 during porcine oocyte maturation     

Yokoo M  Shimizu T  Kimura N  Tunjung WA  Matsumoto H  Abe H  Sasada H  Rodriguez-Martinez H  Sato E 《The Journal of reproduction and development》2007,53(2):263-270

Previous our studies have shown that CD44, the principal receptor for hyaluronan, is present on cumulus cells during oocyte maturation. Although hyaluronan-CD44 interaction has been implicated in cumulus expansion and/or oocyte maturation, the full significance of CD44 remains unknown. The objective of the present study was to further investigate the role of CD44 in cumulus expansion and oocyte maturation in pigs. We demonstrate here in that CD44 has a key role in oocyte maturation but not in cumulus expansion. Previous studies have reported the physiological significance of cumulus expansion in oocyte maturation. However, our results suggest that cumulus expansion is a necessary condition for oocyte maturation, but that it is not sufficient on its own. Furthermore, western blot analysis demonstrated that the CD44 of the in vitro-matured cumulus-oocyte complexes (COCs) had a larger molecular weight and more terminal sialic acid, which has been proven to inhibit the hyaluronan-binding ability of the receptor, than the CD44 of the in vivo-matured COCs, indicating that the hyaluronan-CD44 interactions during in vitro maturation might be insufficient compared with those in vivo. The insufficient interactions of hyaluronan-CD44 during in vitro maturation may cause the inferior capacity of fertilization and development of oocytes matured in vitro.  相似文献   

Identification of cDNA for the HSPA2, HSPA5 and HSPA8 orthologs of the heat shock protein 70 family from guinea fowl (Numida meleagris)     

Shigehisa IWAMOTO  Yohei KOIKE  Kazuyoshi HOSOMICHI  Hiromi HARA  Yutaka YOSHIDA  Hiroshi OGAWA  Kei HANZAWA 《Animal Science Journal》2005,76(5):519-524

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Proportions of melanocyte stimulating hormone-immunoreactive cells in the adenohypophysis of Silky fowl and hyperpigmentation-free cockerels     

Shotaro NISHIMURA  Hitomi SHIMODA  Ichiro OSHIMA  Yoshitaka ONO  Kaoru OKANO  Akira ISHIBASHI  Shoji TABATA  Hisao IWAMOTO 《Animal Science Journal》2005,76(6):575-579

Alpha‐melanocyte stimulating hormone (α‐MSH) is one of the main regulators for melanocytes, and the adenohypophysis is one of the major tissues for the synthesis of this hormone. The Silky fowl is a characteristic breed of chicken with hyperpigmentation throughout the body. The involvement of the adenohypophysis in the hyperpigmentation of this breed is not known. In the present study, the proportion of melanocyte stimulating hormone‐immunopositive cells (MSH cells) in the adenohypophysis was immunocytochemically compared between Silky, Red Cornish × New Hampshire (RN) crossbred and Japanese bantam cockerels at 15 weeks of age. After the body and gland were weighed, the adenohypophyseal cells were enzymatically dispersed and immunostained for α‐MSH, and the immunopositive MSH cells were counted. The weights of the body and adenohypophysis were heaviest in the RN crossbred, followed by the Silky and lightest in the bantam cockerels. In contrast, the ratio between adenohypophysis and bodyweight was much larger in the Silky and the bantam than in the RN crossbreed (P < 0.05). The population of MSH cells in the adenohypophysis was larger in the Silky (14.3%) than in the RN crossbreed (8.0%) and the bantam (8.1%) cockerels (P < 0.05). From these results, it was concluded that prepubertal Silky cockerel have numerous MSH cells in the adenohypophysis suggesting a relationship to hyperpigmentation.  相似文献   

Developmental changes in pituitary–thyroid axis, and formation of gonads in leptocephali and glass eels of Anguilla spp.     

Yuichi Ozaki  Hiromi Okumura  Yukinori Kazeto  Toshitaka Ikeuchi  Shigeho Ijiri  Masaki Nagae  Shinji Adachi and  Kohei Yamauchi 《Fisheries Science》2000,66(6):1115-1122

SUMMARY: Pituitary, thyroid gland and gonads in leptocephali of Japanese eel Anguilla japonica (19.8–32.6 mm in total length), A. obscura (45.0 mm), and A. bicolor pacifica (49.5 mm) and those in glass eels of the Japanese eel were histologically and immunohistochemically examined in order to observe the developmental changes of these endocrine organs in the Anguillidae. The pituitary, consisting of adenohypophysis and neurohypophysis in Japanese eel leptocephali over 22.5 mm, did not contain thyroid stimulating hormone (TSH) immunoreactive cells. Such cells were, however, detectable in the more developed pituitaries of leptocephali of A. obscura and A. bicolor pacifica and in those of glass eels. Conversely, thyroxine (T₄)-immunoreactive thyroid follicles could be detected in all specimens, both leptocephalic and glass eel. Only in glass eels, gonads were found in the body cavity, and these gonads harbored one or two primordial germ cells (PGC) per cross-section. Our results indicate that thyroid hormones (TH) production started prior to TSH production, and that TSH and TH are both secreted during the metamorphosis from leptocephalus to glass eel. Therefore, it is plausible that the TSH–TH axis is involved in the metamorphosis from leptocephalus to glass eel, but not in the early growth from preleptocephalus to leptocephalus.  相似文献   

Seven-year changes in growth and crown shape of Thujopsis dolabrata var. hondai saplings after release from suppression     

Gaku Hitsuma  Takayuki Ota  Tatsuo Kanazashi  Takashi Masaki 《Journal of Forest Research》2006,11(5):387-387

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Long-term growth analyses of Japanese cedar trees in a plantation: neighborhood competition and persistence of initial growth deviations     

Takashi Masaki  Shigeta Mori  Takuya Kajimoto  Gaku Hitsuma  Satoshi Sawata  Masuo Mori  Katsuhiro Osumi  Shobu Sakurai  Takeshi Seki 《Journal of Forest Research》2006,11(4):217-225

The individual growth of tree diameter at breast height (dbh) is analyzed in an even-aged plantation of Cryptomeria japonica from stand age of 45 to 94 years, to examine how the growth of individual trees has been affected by the changes in spacing resulting from thinning operations. At any age, a significant proportion (0.37–0.46) of the variation in dbh growth during a 5–11-year period was explained by dbh at the beginning of the period, probably due to greater leaf mass of larger trees. Next, either one-sided or two-sided competition was added to the model, by calculating the basal area (BA) of neighboring trees around each tree within a given radius or BA for trees having larger dbh than the focal tree within the radius. After preliminary analyses, a radius of 8 m was selected as the critical range for tree competition. Although both types of competition explained a significant proportion (0.09–0.43) of growth variation, one-sided competition was not significant at ages greater than 54 years. Based on the model at 45 years of age, the initial deviation of growth rate for each tree from the predicted rate was calculated and added to the models as a third variable. This raised the coefficient of determination up to 0.50–0.74. These findings have practical significance for forest plantation management, particularly for controlling the growth of standing trees via thinning, to produce high-quality timber in the future.  相似文献   

Dynamic excitation and static loading tests of glulam lattice floor     

Masaki?HaradaEmail author  Kohei?Komatsu  Tomoyuki?Hayashi  Masahiko?Karube 《Journal of Wood Science》2004,50(5):450-454

A wooden lattice floor with high stiffness and damping capacity has been developed to solve noise problems in wooden apartment houses. The lattice floor consisted of Douglas fir glulam beams with inserted steel plate joints and drift pins. To examine the structural performance of the floor, dynamic excitation and static loading tests were conducted on the full size floor. The first and second order resonance frequencies of the floor were 13.5Hz and 27.0Hz, respectively. These frequencies are similar to the peak frequency of a conventional wooden floor and the combined floor fabricated from glued laminated timber and iron. The maximum static load of the floor was 127kN. The apparent flexural rigidity was less than half the value of several floors studied in the past. However, it is considered that the stiffness is improved by constructing panels and this floor has almost equivalent performance. Relative deflection was not affected by the loading history.Part of this study was presented at the International Wood Engineering Conference, New Orleans, October 1996.  相似文献