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M.C. Mato 《Soil biology & biochemistry》1976,8(1):33-35
Humic-like substances synthesized by Aspergillus sydowi, Epicoccum nigrum, Hendersonula toruloidea and Stachyhotrys atra inhibited indoleacetic acid (IAA) destruction by lentil roots extracts. The fungus polymers were similar to soil humic acids with respect to the effect on IAA-oxidase activity. Model polymers prepared using pure phenols reduced the IAA oxidation too. The relationships between the effect of fungal substances on IAA-oxidase activity and their phenolic units content are briefly discussed. 相似文献
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Isolation and identification of goose parvovirus in the UK 总被引:2,自引:0,他引:2
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Mato I Huidobro JF Simal-Lozano J Sancho MT 《Journal of agricultural and food chemistry》2006,54(5):1541-1550
A rapid capillary zone electrophoresis (CZE) method with direct ultraviolet (UV) detection has been set up and developed to determine the most important nonaromatic organic acids in honey with a really simple treatment of the sample. The determination of oxalic, formic, malic, succinic, pyruvic, acetic, lactic, citric, and gluconic acids has been carried out in 4 min. The electrolyte composition was phosphate as the carrier buffer (7.5 mM NaH(2)PO(4) and 2.5 mM Na(2)HPO(4)), 2.5 mM tetradecyltrimethylammonium hydroxide (TTAOH) as electroosmotic flow modifier, and 0.24 mM CaCl(2) as selectivity modifier, with the pH adjusted at 6.40 constant value. The running voltage was -25 kV at a thermostated temperature of 25 degrees C. The injections were performed in hydrodynamic mode (30 s), and the detection mode was UV direct at 185 nm. Validation parameters of the method as detection and quantification limits, linearity, precision (repeatability and reproducibility), and recovery were also studied. The advantages related to the technique such as simplicity, short analysis times, and low consumption of chemicals as well as the good validation parameters obtained for this method permit it to be considered as adequate for routine analysis in honey. 相似文献
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A. NavarreteR.B. Mato G. DimitrakisE. Lester J.R. RobinsonM.J. Cocero S. Kingman 《Industrial Crops and Products》2011,33(3):697-703
Essential oils are important in the flavour and cosmetic industries. Recently, microwave energy has been applied in different extraction processes to obtain essential oils with quality and yield similar to those obtained using steam distillation, but using less energy and time. The dielectric properties of any given material are of critical importance when seeking to understand its response to applied electromagnetic waves. Nevertheless, data related to aromatic plants are scarce. This paper addresses the measurement and estimation of dielectric properties of herbal matrices as a part of the study of the fundamental phenomenology behind the extraction of essential oils with microwaves. A cavity perturbation system was used to measure the properties of the plant matrix, and dielectric mixture equations were used later to try to reproduce the results obtained. The dielectric properties of partly dried rosemary, rosemary's essential oil and oven-dry plant material were measured at temperatures between 20 °C and 160 °C, and atmospheric pressure, at frequencies of 2450 MHz and 910 MHz. The results show a strong influence of water content on the dielectric properties. A new predictive approach that uses the results from measurements of dielectric properties from the different constituents to obtain effective mixture dielectric properties is proposed. The predicted values of the dielectric constants were better than those of the loss factors. 相似文献
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Evolution of invertase activity in honey over two years 总被引:1,自引:0,他引:1
Sánchez MP Huidobro JF Mato I Muniategui S Sancho MT 《Journal of agricultural and food chemistry》2001,49(1):416-422
Invertase activity is a good parameter for evaluating honey freshness. Invertase activity evolution was determined on 57 fresh, unheated, commercially purchased Galician (northwestern Spain) floral honey samples. All honeys were stored in darkness at room temperature for up 24 months and analyzed each 6 months so as to determine the invertase activity evolution tendency for the first time. Invertase activity analysis was carried out according to Siegenthaler's method and in a simple assay, the latter showing a good precision (coefficient of variation between 0.35 and 0.66%). Initial invertase activity mean value was 163.9 (48.4-251.0) micromol of 4-nitrophenyl-alpha-D-glucopyranoside hydrolyzed/kg of honey/min. After application of the SPSS statistical package, the values of invertase activity showed five types of temporal behavior: exponential (56% of samples), linear (25% of samples), logarithmic (11% of samples), inverse (5% of samples), and quadratic (3% of samples). Linear regression equations were used to predict the invertase activity at 6, 12, 18, and 24 months from the initial Galician honeys' invertase activities; no statistical differences were found between experimental data and the activities calculated from the linear regression equations. 相似文献
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Mirjana Baban Ino Curik Boris Antunovic Mato Cacic Nidal Korabi Pero Mijic 《Journal of Equine Veterinary Science》2009
Besides being well known for their use in classical dressage, Lipizzaner horses are today becoming more and more popular for use in driving sport, with Croatia as the largest breeder in the world. To estimate their phenotypic predisposition for driving sport, this research analyzes the correlation between body measurements and traits of length and speed of walk and hand-led trot in 18 stallions (LS) and 53 mares (LM) by using a digital camera. Statistical analysis of the data concerning phenotypic correlations among the indicated traits has revealed the potential of documenting a large number of structural exterior body characteristics and quantitative measurements in Lipizzaner horses, and technical equipment has been found to be useful in research of stride traits. Higher withers height (WH) achieved through breeding was found to be negatively correlated with the number of strides per second during walk and trot, but positively correlated with walk stride length, especially in LS. Higher values for chest girth (CG) and cannon bone circumference (CBC) were found to be positively correlated with speed, as well as with stride length in LS, and negatively correlated with the number of strides per second during walk and trot in both LS and LM. Because for many years the goal of breeding Lipizzaners in Croatia has been to obtain a bigger body format and longer stride, leading to better results in a driving sport, these findings confirm breeding goals in selection work guided toward better stride length and extended stride as the most desirable traits for driving sport. 相似文献
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Suarez-Luque S Mato I Huidobro JF Simal-Lozano J Sancho MT 《Journal of agricultural and food chemistry》2006,54(25):9292-9296
A capillary zone electrophoresis method for the determination of inorganic anions and formic acid in honey samples was developed for the first time. The complete separation of chloride, nitrate, sulfate, phosphate, and formic acid was achieved with a simple electrolyte composed by 2 mM potassium dichromate as the carrier solution and background absorbance provider and 0.05 mM tetraethylenepentamine (TEPA) as electro-osmotic flow suppressor (pH 4.00). Injection was performed hydrostatically by elevating the sample at 10 cm for 10 s. The running voltage was -27 kV at 25 degrees C. Indirect UV absorption detection was achieved at 254 nm. The detection limit was in the range between 0.03 and 20 mg/kg, and the quantification limits ranged from 1.52 to 20.6 mg/kg. The calibration graphs were linear in the concentration range from the quantification limit to at least 2.5 g/kg for chloride, 0.25 g/kg for nitrate, 0.75 g/kg for sulfate, 1.50 g/kg for phosphate, and 0.75 g/kg for formic acid. Precision data in the honey samples analyzed showed repeatability and reproducibility relative standard deviations lower than 1.4 and 2.4% for migration time and lower than 1.8 and 4.3% for anion content, respectively. Recoveries of anions in honey samples analyzed ranged from 94.4 to 99.8%. Ten honey samples were analyzed to test the proposed method. Mean contents of 260.5, 3.93, 60.5, 139.4, and 209.3 mg/kg were found, respectively, for chloride, nitrate, sulfate, phosphate, and formic acid in analyzed honeys. These results agreed with literature data. 相似文献
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Mato Drenjančević Vladimir Jukić Krunoslav Zmaić Toni Kujundžić 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2017,67(8):705-711
The aim of this study was to determine the impact of two different treatments of early defoliation performed before blooming on: grape yield, chemical parameters, polyphenols content, and antioxidant activity of grape and red wine cv. Cabernet Sauvignon from the vineyard located in Ilok, the eastern continental region of Croatia. Two different treatments of leaf removal (LR) were performed: removal of 3 leafs (T1) and 6 leafs (T2) before blooming, together with control (no leaf removal) (T3) during two years (2013 and 2014). Crop yield and average cluster weights per vine were determined. Density, pH and titratable acidity were measured in must, while the total phenols, total anthocyanins and antioxidant activity were measured in the extract of grape skin and produced wine. The analysis of individual anthocyanins in wine was performed by HPLC method. T2 treatment significantly lowered the crop yield and the average cluster weights, and increased total phenols, total anthocyanins, antioxidant activity and most abundant individual anthocyanins in wine. Defoliation did not affect the other chemical parameters in must, grape skin extract and wine. Vintage year is statistically the most significant source of variability for density of must, antioxidant activity in grape skin extract, as well as pH and titratable acidity in wine. This study has showed that the early leaf removal treatment in eastern continental part of Croatia could be used for the production of smaller quantity of high quality Cabernet Sauvignon red wine abundant with anthocyanins. 相似文献
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