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排序方式: 共有176条查询结果,搜索用时 15 毫秒
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Alghamdi AS Troedsson MH Xue JL Crabo BG 《American journal of veterinary research》2002,63(6):880-885
OBJECTIVE: To compare the effect of semen extender and seminal plasma on postthaw motility and filtration through a glass wool-Sephadex (GWS) filter for frozen stallion semen. SAMPLE POPULATION: 7 stallions from which we collected > or = 3 ejaculates/stallion. PROCEDURES: 4 experiments were conducted to evaluate postthaw quality of frozen stallion semen. Kenney extender was compared with glucose-EDTA extender by use of various dilution rates that resulted in differing concentrations of seminal plasma. Stallions known to produce semen with poor postthaw quality were used to investigate whether a particular extender or dilution rate could improve ability of such semen to survive freeze-thaw procedures. RESULTS: Use of Kenney extender as the centrifugation extender significantly improved postthaw motility and GWS filtration, compared with glucose-EDTA. Extending semen at a dilution of 1:3 was significantly better than 1:1 for both motility and GWS filtration. In addition, including seminal plasma at a concentration of 5% in the cryopreserved semen resulted in significantly higher yield of spermatozoa after GWS filtration, compared with complete removal of SP or use of seminal plasma at 25%. Lastly, semen with poor postthaw quality had significantly improved postthaw quality in regard to motility and GWS filtration when semen was frozen with seminal plasma at a concentration of 5%, compared with semen frozen with seminal plasma at a concentration of 25%. CONCLUSIONS AND CLINICAL RELEVANCE: Use of Kenney extender at a high dilution (> or = 1:3) immediately after collection of semen can improve postthaw quality of frozen stallion semen. 相似文献
4.
Stefan Antonsson Gunnar Henriksson Mats Johansson Mikael E. Lindström 《Industrial Crops and Products》2008,27(1):98-103
Lignin residues are available in large amounts as kraft lignin from chemical pulping processes. This lignin is mainly incinerated in recovery boilers. The recovery boilers are often the bottle-necks in the overall pulping process when pulp production increases are desired. Through cross-flow nano-filtration of the black liquor from kraft pulping, a low-molecular weight lignin fraction can be removed thus decreasing the organic load on the recovery boilers. The low-molecular weight lignin fraction furthermore exhibit different characteristics compared to other commercial kraft lignins and represents a new raw material source in novel applications.The low-molecular weight lignin was used together with a vegetable oil to produce a new hydrophobic lignin derivative similar to suberin. The lignin and the lignin derivative was analysed with FT-IR, UV–vis and SEC. The ability of the product to make paper surfaces hydrophobic was also evaluated.The results demonstrate the possibility to make a suberin-like lignin derivative that is potentially of interest in paper-coating applications due to its capability to interact well with wood fibres and make paper hydrophobic. 相似文献
5.
Elisabeth Lindahl Nosirjon Sattorov Sofia Boqvist Izzatullo Sattori Ulf Magnusson 《Tropical animal health and production》2014,46(3):563-569
In this cross-sectional study, we assessed and mapped the seroprevalence of brucellosis in small-scale dairy farming in an urban and peri-urban area of Tajikistan and investigated factors associated with seropositivity. As urban and peri-urban farming is both an opportunity to improve the livelihood for small-scale farmers and a potential public health hazard, studies are warranted to reveal possible peculiarities in the epidemiology of brucellosis in this type of dairy farming. In total, 904 cows of breeding age belonging to 443 herds in 32 villages were serologically tested with indirect enzyme-linked immunosorbent assay (ELISA) and positive samples confirmed with competitive ELISA. Two logistic regression models were used to investigate an association between seropositivity and risk factors at herd and individual level. The herd and individual seroprevalences were 4.1 and 2.0 %, respectively. Herds with a history of abortions were found to be associated with seropositivity [odds ratio (OR)?=?5.3; 95 % confidence interval (CI), 1.3–21.3]. Large herds with more than eight cattle were more likely to be seropositive compared to smaller herds with one to two cattle (OR?=?13.9; 95 % CI, 1.6–119). The number of calves produced per cow (indicating age) was found to be associated with seropositivity. Younger cows with one to two produced calves were less likely to be seropositive compared to older cows with more than six produced calves (OR?=?0.24; 95 % CI, 0.06–1.0). Neither introduction of new cattle to the herd nor communal grazing was associated with seropositivity. This study shows that infection with Brucella (1) is present in small-scale urban and peri-urban dairy farming in Tajikistan and (2) has significant negative effects on reproductive performance in this farming system and (3) that some previously known risk factors for seropositivity in rural farming system were absent here. 相似文献
6.
Katheryn L. Cerny Tom V. Little Charles F. Scoggin Robert J. Coleman Mats H.T. Troedsson Edward L. Squires 《Journal of Equine Veterinary Science》2014
The current field study used thoroughbred stallions and mares from central Kentucky to investigate the occurrence of potentially pathogenic bacteria on the stallion's external genitalia, based on cultures, and investigated the occurrence of bacteria and type of isolate in the mare's uterus after breeding by live cover to stallions with or without positive bacterial cultures. Fifteen thoroughbred stallions and 206 mares from two central Kentucky thoroughbred farms were used during the 2010 and 2011 breeding seasons. Samples for bacteriological evaluation were taken from the prepuce and postejaculate urethra (n = 201) of stallions. Uterine swabs (n = 264) were collected 12-18 hours postbreeding. For statistical analyses, a chi-squared test was used to test the relationship between stallion culture results and postbreeding uterine culture results, as well as the effect of bacterial types found on the stallion cultures with bacterial types found on the postbreeding uterine cultures. Of stallion cultures, 22.4% were positive for potentially pathogenic bacteria, with Streptococcus equi subspecies zooepidemicus (51.1%) being the most common isolate. Uterine cultures resulted in a 29.2% positive rate for potentially pathogenic bacteria, with S. equi subsp. zooepidemicus (90.9%) being the most common. There was no difference (P > .05) in the occurrence of bacteria or type of isolate found on uterine cultures after breeding stallions with or without positive cultures. In conclusion, potentially pathogenic bacteria found on the stallion's external genitalia did not affect the occurrence and type of bacterial isolate found in the mare's uterus after breeding by live cover. 相似文献
7.
Six Swedish and one Canadian single spore isolate of Drechslera teres f. teres were used to screen 109 barley lines for disease
resistance and to select a differential set of barley lines for use in assessing pathogen virulence. A large variation for
net blotch resistance was found among the 109 barley lines which were classified into four groups, those showing: 1) only
resistant reactions; 2) differential reactions; 3) only intermediate reactions and 4) only susceptible reactions. The European
commercial varieties included, showed susceptibility to all Swedish isolates, but a few were resistant to the Canadian isolate.
The 18- member differential set separated 25 Swedish and two Canadian isolates of D. teres into 14 pathotypes, three of which
made up 59% of the isolates. Only one barley differential (CI 9776) was resistant to all net form isolates. Host selection
on the pathogen seems to be present as all six isolates obtained from cv. Golf belonged to the same pathotype and 4 of 5 isolates
from cv. Karin shared the same virulence pattern. The net form of net blotch (D. teres f. teres) predominated in the sampled
regions and only one of 26 Swedish isolates was of the spot form (D. teres f. maculata).
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
8.
Mats Aastrup Åke Iverfeldt Lage Bringmark Hans Kvarnäs Bo Thunholm Hans Hultberg 《Water, air, and soil pollution》1995,85(2):755-760
Long-term integrated catchment monitoring within the Swedish Environmental Monitoring Programme (PMK) aims primarely at the fate and effects of pollutants in mature ecosystems, mainly protected boreal forests. The measurements that were conducted since the early eighties, with some variation, included deposition, throughfall, litterfall and the chemistry of soil water, groundwater and runoff water.Together with, or perhaps partly due to acidification, accumulation and transport of heavy metals are a serious threat to the ecosystem and in the end to the health of human beings. This paper discuss the behaviour of Hg, Pb, Cd, Zn and Cu based on the experiece gained from the studies in the small catchment areas. The importance of present deposition, soil storage and biogeochemical condition for metal mobility to the transport of metals is discussed The storages of Hg and Pb have been built up during a long time span and are very large in comparison with the flux. The present atmospheric deposition have probably little impact on the flux, which rather is governed by the biogeochemical conditions. There is still a small accumulation of Cd, but its great mobility under acidic conditions makes input and output almost in balance. A reduction in deposition will probably have an immediate effect on flux, which also is the case for Zn. Cu generally accumulates in soil. The mobility and hence the flux are certainly regulated by the biogeochemical conditions, but more detailed studies are required to reveal the general mecanisms for Cu transport within the catchment. 相似文献
9.
Lars Lundin Mats Aastrup Lage Bringmark Sven Bråkenhielm Hans Hultberg Kjell Johansson Karin Kindbom Hans Kvarnäs Stefan Löfgren 《Water, air, and soil pollution》2001,130(1-4):1031-1036
Integrated monitoring of ecosystems (IM) is an international co-operative programme (ICP) to control effects of air pollution and climate change on water, soil and biological systems. It is a part of the Convention on Long-Range Transboundary Air Pollution (CLRTAP) of the United Nations Economic Commission of Europe (UN/ECE). The ICP-IM is undertaken on sites/catchments to investigate acidification, eutrophication and heavy metals with an integrated approach. In Sweden, long-term time series from forest ecosystems, with a long and stable continuity, will reveal trends and changes in processes and enable modelling to be undertaken. Investigations of acidity/alkalinity in relation to mineral and organic acids indicated the importance of atmospheric deposition. Recent results show very high inorganic nitrogen retention (99%), a net loss of sulphur originating mainly from organic horizons, and a high inorganic aluminium content in the illuvial soil horizons which could be detrimental to forests. Forest deficiency could also be caused by an observed ongoing translocation of Zn to deeper soil layers implying a movement towards increased release to surface waters. 相似文献
10.
Regulation by growth hormone of number of chondrocytes containing IGF-I in rat growth plate 总被引:12,自引:0,他引:12
A Nilsson J Isgaard A Lindahl A Dahlstr?m A Skottner O G Isaksson 《Science (New York, N.Y.)》1986,233(4763):571-574
Whether growth hormone stimulates longitudinal bone growth by a direct effect at the site of the growth plate or indirectly by increasing the concentration of circulating somatomedins (insulin-like growth factors) has been the subject of controversy. Immunohistochemical methods were used to explore the localization and distribution of insulin-like growth factor I (IGF-I) immunoreactivity in the epiphyseal growth plate of the proximal tibia of male rats. Cells in the proliferative zone of the growth plate of normal rats exhibited a bright immunofluorescence, whereas cells in the germinal and hypertrophic zones stained only weakly. In rats subjected to hypophysectomy, the number of fluorescent cells was markedly reduced. When the hypophysectomized rats were treated with growth hormone, either systemically or at the site of the growth plate, the number of IGF-I-immunoreactive cells in the proliferative zone was increased. The results show that IGF-I is produced in proliferative chondrocytes in the growth plate and that the number of IGF-I-containing cells is directly regulated by growth hormone. These findings suggest that IGF-I has a specific role in the clonal expansion of differentiated chondrocytes and exerts its function locally through autocrine or paracrine mechanisms. 相似文献