贻贝采卵试验报告     

楼子康  刘祥生  何义朝  李淑英  张福绥  陈昭华  张秀峰  张乃石 《水产学报》1982,6(2):139-146

为了探讨贻贝人工育苗的规模、数量、茬次等问题，本文对采卵的规律进行了研究。通过在不同季节和时期用变温刺激法来诱导排卵试验，认为在春季应用升温为主的常规综合刺激法，其排卵宁可达到60%以上；在秋季应以降温为主的变温刺激法，排卵率可达到40-60%。此外，文中对不同年龄的亲贝所排放生殖细胞的雌雄比例和采卵量等问题进行了讨论。  相似文献   

Thermal preference and tolerance of green abalone Haliotis fulgens (Philippi, 1845) and pink abalone Haliotis corrugata (Gray, 1828)     

Fernando Díaz  Ana Denisse Re  Zarina Medina  Gerardo Re  Gustavo Valdez  & Francisco Valenzuela 《Aquaculture Research》2006,37(9):877-884

The thermoregulatory behaviour of green abalone Haliotis fulgens and pink abalone H. corrugata was investigated. Haliotis fulgens juveniles ranging in wet weight from 3.0 to 3.3 g and from 28.7 to 30.5 mm shell length and of H. corrugata 2.0 g and 25.7 mm in shell length were exposed to 19°C for 30 days in a flow‐through water system. Temperature preference was determined in a horizontal thermal gradient and was found to be 25.4°C for green abalone and 25.0°C for pink abalone. Displacement velocity was 4.3 cm h⁻¹ for H. fulgens and 12.8 cm h⁻¹ for H. corrugata. The optimum temperature for growth calculated for both abalone species was 24.6 and 24.5°C respectively. The critical thermal maxima (CTMax) of H. fulgens and H. corrugata were determined as a measure of thermal tolerance. Abalones were subjected to increasing water temperatures at a rate of 1°C on 30 min until they detached from the substrate. The CTMax at 50% were 33.6 and 32.0°C for green and pink abalone respectively. The results are discussed in relation to site selection and commercial rearing.  相似文献   

Survey and new insights in the application of PCR‐based molecular markers for identification of HMW‐GS at the Glu‐B1 locus in durum and bread wheat          下载免费PDF全文

Michela Janni  Domenico Pignone  Nelson Marmiroli 《Plant Breeding》2017,136(4):467-473

Wheat, among all cereal grains, possesses unique characteristics conferred by gluten; in particular, high molecular weight glutenin subunits (HMW‐GS) are of considerable interest as they strictly relate to bread‐making quality and contribute to strengthening and stabilizing dough. Thus, the identification of allelic composition, in particular at the Glu‐B1 locus, is very important to wheat quality improvement. Several PCR‐based molecular markers to tag‐specific HMW glutenin genes encoding Bx and By subunits have been developed in recent years. This study provides a survey of the molecular markers developed for the HMW‐GS at the Glu‐B1 locus. In addition, a selection of molecular markers was tested on 31 durum and bread wheat cultivars containing the By8, By16, By9, Bx17, Bx6, Bx14 and Bx17 Glu‐B1 alleles, and a new assignation was defined for the ZSBy9_aF1/R3 molecular marker that was specific for the By20 allele. We believe the results constitute a practical guide for results that might be achieved by these molecular markers on populations and cultivars with high variability at the Glu‐B1 locus.  相似文献   

Efficacy of Chlorhexidine against Some Strains of Cultured and Clinically Isolated Microorganisms     

Odore R  Colombatti Valle V  Re G 《Veterinary research communications》2000,24(4):229-238

The efficacy of chlorhexidine digluconate was determined against some strains of collected and clinically isolated bacteria and fungi. The efficacy was evaluated either by calculating a minimum inhibitory concentration (MIC) or by efficacy trials according to the guidelines of the European Committee for Standardization. The MIC values of chlorhexidine for Staphylococcus aureus, Microsporum gypseum, Microsporum canis and Trichophyton mentagrophytes were 0.625 g/ml, 12.5 g/ml, 50 g/ml and 6.25 g/ml, respectively. The in vitro efficacy of chlorhexidine was higher against ATCC strains of S. aureus and P. aeruginosa (0.5 mg/ml for 5 min and 0.5 mg/ml for 10 min, respectively) than against clinical isolates (0.5 mg/ml for 15 min and 1 mg/ml for 10 min, respectively). The antiseptic activity of aqueous solutions of chlorhexidine against spores of Bacillus subtilis, Bacillis sfericus and Clostridium perfringens required longer contact times than against the vegetative forms. Nevertheless, 5 mg/ml of chlorhexidine in water–ethanol 20:80 v/v was totally effective against the vegetative forms or spores of these microorganisms.  相似文献   

Infant feeding practices in Xinjiang Uygur Autonomous Region, People's Republic of China     

Xu F  Binns C  Wu J  Yihan R  Zhao Y  Lee A 《Public health nutrition》2007,10(2):198-202

AIMS: To document infant feeding methods in the first six months of life in Xinjiang Uygur Autonomous Region, People's Republic of China, 2003-2004. Some problems with breast-feeding in the area are explained. METHODS: A longitudinal study of infant feeding practices was undertaken. A total of 1219 mothers who delivered babies during 2003 and 2004 were interviewed in five hospitals or institutes, and after discharge were contacted in person or by telephone at approximately monthly intervals to obtain details of infant feeding practices. Multivariate logistic regression analysis was used to explore factors associated with breast-feeding initiation. RESULTS: 'Any breast-feeding' rates at discharge and at 0.5, 1.5, 2.5, 3.5, 4.5 and 6 months were 92.2, 91.3, 89.9, 88.8, 87.7, 86.0 and 73.0%, respectively. 'Exclusive breast-feeding' rates at discharge and at 0.5, 1.5, 2.5, 3.5, 4.5 and 6 months were 66.2, 47.6, 30.1, 25.8, 22.1, 13.0 and 6.2%, respectively. The main problem of breast-feeding in Xinjiang was the early introduction of formula or water. The average duration of 'exclusive breast-feeding' was 1.8 months (95% confidence interval (CI) 1.7-2.0), of 'full breast-feeding' 2.8 months (95% CI 2.7-2.9) and of 'any breast-feeding' 5.3 months (95% CI 5.2-5.4). CONCLUSIONS: Infant feeding methods in Xinjiang were documented in this study and the main problems with infant feeding in Xinjiang are discussed. Further studies are needed to identify factors associated with 'exclusive breast-feeding' and duration.  相似文献   

In vitro enantioselective pharmacodynamics of Carprofen and Flunixin‐meglumine in feedlot cattle     

M. Miciletta  B. Cuniberti  R. Barbero  G. Re 《Journal of veterinary pharmacology and therapeutics》2014,37(1):43-52

The activity of the anti‐inflammatory agents Flunixin‐meglumine (FLU), RS (±) Carprofen (CPF) and S (+) CPF on bovine cyclooxygenases (COXs) has been characterized in feedlot calves using an in vitro whole blood model. The drugs showed equivalent efficacy in their inhibitory activity on COXs, and the rank order of potency for both COX‐1 and COX‐2 inhibition was FLU > S (+) CPF > RS (±) CPF. Our results indicated that FLU is a nonselective inhibitor of bovine COXs, whereas RS (±) CPF and S (+) CPF exhibited different degrees of preferential inhibition of COX‐2 isoenzyme. The rank order of IC₅₀ COX‐1: IC₅₀ COX‐2 potency ratios was in fact S (+) CPF (51.882) > RS (±) CPF (13.964) > FLU (0.606), and the calculated percentage inhibition of COX‐1 corresponding to COX‐2 inhibition values comprised between 80% and 95% was comprised between 57.697 and 79.865 for FLU, 33.373 and 51.319 for RS (±) CPF, and 0.230 and 4.622 for S (+) CPF, respectively. These findings are discussed in relation to the prediction of the clinical relevance of COX inhibition by the test drugs in cattle.  相似文献   

Subependymal Reaction Secondary to Choroid Plexus Papilloma in a Horse     

Paulo Ricardo Dell'Armelina Rocha  Andrea Bertuglia  Elena Biasibetti  Michela Bullone  Alfonso Rosamilia  Loretta Masoero  Maria Teresa Capucchio 《Journal of Equine Veterinary Science》2014

Choroid plexus papilloma (CPP) is a rare, benign, intracranial neoplasm. The present report described a choroid plexus papilloma of the fourth ventricle with unusual proliferative reaction of ependymal/subependymal cells in a stallion. A single, soft neoformation, red-gray in color, with an irregular surface, resembling a cauliflower, expanding from the fourth ventricle to the cerebellar peduncles was observed. Microscopically, the neoformation was composed of a single layer of simple cuboidal to columnar epithelium with a well-vascularized papillary-arboriform pattern. Multifocal subependymal aggregates forming rosettes were observed near the mesencephalic aqueduct (MA). Glial-fibrillary-acidic-protein (GFAP) was markedly positive only in the neuropil adjacent to the proliferative areas of the MA. Ependymal/subependymal reactions secondary to CPP have never been described in equine or human neuropathology. The capabilities of ependymal cells to regenerate and eventually proliferate are controversial and unclear and, thus, require additional investigation to understand the adaptive mechanisms of this rare pathogenic condition.  相似文献   

New contribution on the distribution and ecology of Dendrophyllia ramea (Linnaeus, 1758): abundance hotspots off north-eastern Sicilian waters     

Eva Salvati  Michela Giusti  Simonepietro Canese  Valentina Esposito  Teresa Romeo  Franco Andaloro  Marzia Bo  Leonardo Tunesi 《水产资源保护:海洋与淡水生态系统》2021,31(6):1322-1333

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NaCl胁迫对阿克苏鹰嘴豆种子萌发特性的影响     

毛居代  买热木古丽  原慧  朱江梅 《种子》2011,30(11)

用不同浓度的NaCl胁迫处理鹰嘴豆种子,并研究了NaCl胁迫下鹰嘴豆种子萌发的特性.结果表明,随着盐浓度的升高,根重、根长、根干重、芽重、芽长、芽干重均呈下降趋势,低浓度(10～ 50 mmol/L)可促进种子萌发,尤其是在50 mmol/L时生长最好;而高浓度的NaCl(100～600 mmol/L)抑制种子萌发,和对照组相比有显著性的差异.甚至在400～600 mmol/L浓度下鹰嘴豆种子不萌发.  相似文献   

Equivalent effects of snake PLA2 neurotoxins and lysophospholipid-fatty acid mixtures   总被引：1，自引：0，他引：1  

Rigoni M  Caccin P  Gschmeissner S  Koster G  Postle AD  Rossetto O  Schiavo G  Montecucco C 《Science (New York, N.Y.)》2005,310(5754):1678-1680

Snake presynaptic phospholipase A2 neurotoxins (SPANs) paralyze the neuromuscular junction (NMJ). Upon intoxication, the NMJ enlarges and has a reduced content of synaptic vesicles, and primary neuronal cultures show synaptic swelling with surface exposure of the lumenal domain of the synaptic vesicle protein synaptotagmin I. Concomitantly, these neurotoxins induce exocytosis of neurotransmitters. We found that an equimolar mixture of lysophospholipids and fatty acids closely mimics all of the biological effects of SPANs. These results draw attention to the possible role of local lipid changes in synaptic vesicle release and provide new tools for the study of exocytosis.  相似文献