Risk factors for bit-related lesions in Finnish trotting horses     

Kati Tuomola  Nina Mäki-Kihniä  Anna Valros  Anna Mykkänen  Minna Kujala-Wirth 《Equine veterinary journal》2021,53(6):1132-1140

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Matrix metalloproteinase‐2, ‐7, and ‐9 activities in dogs with idiopathic pulmonary fibrosis compared to healthy dogs and dogs with other respiratory diseases     

Merita Mtt  Henna P. Laurila  Saila Holopainen  Kaisa Aaltonen  Liisa Lilja‐Maula  Sanna Viitanen  Minna M. Rajamki 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2021,35(1):462-471

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睾丸支持细胞的功能、分离纯化及鉴定研究进展     

伊敏娜  才文道力玛  陶力  王希生  神英超  杜明  任宏  芒来  格日乐其木格 《中国畜牧兽医》2021,48(8):2947-2956

支持细胞对维持精子形成过程中的微环境起决定作用,它可以通过分泌功能、细胞间连接形成的血睾屏障功能以及吞噬功能等来促进精子的形成过程,其发育异常会导致不同程度的雄性生殖缺陷。基于支持细胞在雄性动物生殖过程中的作用,体外培养高纯度支持细胞可成为研究睾丸两大核心功能-精子发生和性激素分泌功能相关调节机制重要的细胞模型。此外,体外培养睾丸支持细胞也可作为生殖毒理学等新兴热点领域的细胞模型,为评估和研究环境因素对雄性生殖的影响提供便利。因此,作者系统地归纳、总结了目前关于动物支持细胞生物功能的研究及常用的体外分离纯化、培养及鉴定方法,以期为利用动物支持细胞开展雄性生殖领域的研究提供参考。  相似文献   

High performance liquid chromatography determination of ergosterolas a measure of ectomycorrhiza infection in Scots pine     

By B. Salmanowicz  J.-E. Nylund 《Forest Pathology》1988,18(5):291-298

A technique for determining the ergosterol content in mycorrhizal pine roots using HPLC was developed. Pure cultures of different my corrhizal fungi contained very similar and constant amounts of ergosterol, and calculations of fungal biomass in mycorrhizal roots based on ergosterol readings agreed well with results using other methods. The extraction and sample purification were simple and reliable. Consequently, the technique is considered practical wherever accurate estimates of the intensity of mycorrhizal infection are required.  相似文献   

Simple diagnosis of ectomycorrhiza formation and demonstration of the architecture of the Hartig net by means of a clearing technique     

J.-E. Nylund  Asa Kasimir  Agneta Strandberg Arveby  T. Unestam 《Forest Pathology》1982,12(2):103-107

Coniferous ectomycorrhiza was cleared in KOH and bleached in H₂ 0₂ . After the treatment, the structure of the Hartig net and inner mantle could be studied in detail in entire mycorrhizal short roots. The Hartig net and inner mantle were found to be formed by the same type of labyrinthic tissue, a result of morphogenetic processes during mycorrhiza formation. Even early phases of mycorrhizal infection could easily be detected, which makes the method very suitable for diagnosticating mycorrhiza formation accurately.  相似文献   

Identification of Matrix Metalloproteinase-2 and -9 Activities within Intestinal Mucosa of Clinically Healthy Beagle Dogs     

Mohsen HANIFEH  Minna M RAJAM?KI  Laura M?KITALO  Pernilla SYRJ?  Satu SANKARI  Susanne KILPINEN  Thomas SPILLMANN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(8):1079-1085

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The combined effects of enhanced UV-B radiation and selenium on growth, chlorophyll fluorescence and ultrastructure in strawberry (Fragaria × ananassa) and barley (Hordeum vulgare) treated in the field     

Elena Valkama  Minna Kivimenp  Helin Hartikainen  Anu Wulff 《Agricultural and Forest Meteorology》2003,120(1-4):267

The possible ameliorative effects of selenium (Se) addition to soil on the detrimental effects of enhanced UV-B radiation were tested on strawberry and barley during 4 months of field experiment in Kuopio, Central Finland. Control plants were exposed to ambient levels of UV radiation, using arrays of unenergized lamps. A control for UV-A radiation was also included in the experiment. Added Se, applied as H₂SeO₄, at the level of 0.1 mg kg⁻¹ soil (low dosage) and 1 mg kg⁻¹ soil (high dosage) increased Se concentrations in plants more than 10 and 100 times, respectively. After 4 months of exposure, strawberry and barley plants were harvested for biomass analysis. Chlorophyll fluorescence was measured using the Hansatech FMS2 fluorescence monitoring system. Leaf anatomy and ultrastructure were observed by light and transmission electron microscope. Several effects of UV and Se as well as their interaction were found, mostly for strawberry, but not for barley, indicating species-specific responses. Our results provided evidence that the high Se concentration in soil had no ameliorative effect but increased the sensitivity of strawberry to enhanced UV-B radiation in the field. Under ambient radiation, Se did not alter leaf growth of strawberry, whereas under UV-B radiation, the high Se addition significantly decreased leaf growth. Strawberry runner biomass was affected by the interaction of Se and UV. Under ambient radiation Se did not change dry weight of runners, but in combination with UV-A or UV-B radiation the high Se dosage decreased dry weight of runners by about 30%. Although the high Se concentration positively influenced on quantum efficiency of photosystem II (PSII) in strawberry leaves, it reduced runner biomass, leaf number and ratio of starch to chloroplast area. This suggests that the harmful effects of the high Se dosage on photosynthetic processes occurred as a result of changes in activity or/and biosynthesis of enzymes, rather than alteration of PSII. At the low concentration, Se effects were slight and variable.Although barley leaves accumulated higher Se concentrations than strawberry, there were no apparent changes in their growth, biomass or chlorophyll fluorescence due to Se effect either alone or in combination with UV-B. However, at the ultrastructural level, an enlargement in the peroxisome area was found due to combination of UV radiation with Se, suggesting the activation of antioxidative enzymes, possibly catalase. Decrease in mitochondrial density in barley cells in response to Se might be attributed to alteration of mitochondrial division. Increase in the proportion of cells with cytoplasmic lipid bodies due to combined effect of UV-B and Se indicated the alteration of lipid metabolism and the acceleration of cell senescence in barley. Main UV-B effects were found, mostly at the tissue and ultrastructural level in strawberry, but not in barley, indicating species-specific susceptibility to enhanced UV-B radiation. UV-B-treated strawberry plants developed marginally thinner leaves with reduced ratio of starch to chloroplast area in their cells, suggesting negative influence of UV-B on photosynthetic processes.  相似文献   

MED12, the mediator complex subunit 12 gene, is mutated at high frequency in uterine leiomyomas     

Mäkinen N  Mehine M  Tolvanen J  Kaasinen E  Li Y  Lehtonen HJ  Gentile M  Yan J  Enge M  Taipale M  Aavikko M  Katainen R  Virolainen E  Böhling T  Koski TA  Launonen V  Sjöberg J  Taipale J  Vahteristo P  Aaltonen LA 《Science (New York, N.Y.)》2011,334(6053):252-255

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Congestive heart failure and atrial fibrillation in a cat with myocardial fibro-fatty infiltration     

Harjuhahto TA  Leinonen MR  Simola OT  Järvinen AK  Rajamäki MM 《Journal of Feline Medicine and Surgery》2011,13(2):109-111

Congestive heart failure and atrial fibrillation were diagnosed in a 4-year-old castrated Birman cat with progressive signs of dyspnea, tachypnea, and lethargy. Echocardiography revealed massive right-sided heart dilatation with ascites and hydrothorax. Electrocardiogram recording showed atrial fibrillation. Medical therapy with diuretics, benazepril, and antithrombotic agents was unsuccessful. The owner requested euthanasia. In post-mortem examination, changes associated with myocardial fibro-fatty infiltration were confirmed. Changes were most marked in the right ventricular wall but with left ventricular involvement was detected.  相似文献   

Peat Biofilters in Long-Term Experiments for Removing Odorous Sulphur Compounds   总被引：1，自引：0，他引：1  

Tarja Hartikainen  Pertti J. Martikainen  Minna Olkkonen  Juhani Ruuskanen 《Water, air, and soil pollution》2002,133(1-4):335-348

Peat was employed as a biological filter material for the removal of hydrogen sulphide (H_2> S) dimethyl sulphide (Me₂S) and methyl mercaptan (MeSH) from odorous ventilation air. Removal of H₂S was possible without the need to inoculate the filter material with oxidizing microbes whereas Me₂S required inoculation for degradation. The removal of Me₂S was decreased as a result of MeSH addition. Liming of the filter material, i.e., addition of calcium hydroxide, increased removal efficiency and activated the oxidation of H₂S without any adaptation period. As a consequence of the poor Me₂S removal efficiency of natural peat, a limed and inoculated biofilter was needed to purify mixtures of gases containing sulphur. The highest H₂S load tested with limed but otherwise natural peat was 136 g-S m^-3 day^-1, yielding a 99 % reduction. The maximum Me₂S elimination capacity with limed and inoculated peat was 175 g-S m^-3day^-1, but removal became unstable when the load exceeded 150 g-S m^-3day^-1. The maximum MeSH load tested was 107 g-S m^-3day^-1, yielding 98 % removal.  相似文献