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Toshitaka KANEI Munetaka IWATA Hiroaki KAMISHINA Takuya MIZUNO Sadatoshi MAEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):25
C-C chemokine receptor 7 (CCR7) contributes to cell homing to lymph nodes (LNs). Recent studies reported that CCR7 is also expressed in tumor cells, which correlates with LN metastasis in various cancers. However, the expression of CCR7 in tumor cells is unknown in dogs due to the lack of appropriate antibodies. In the present study, a fusion protein of C-C chemokine ligand 19 (CCL19) was employed as an alternative method to CCR7 antibodies. The fusion CCL19 protein specifically detected CCR7 expressed in canine lymphoma cell lines, which showed active chemotaxis to both canine and mouse ligands. The present study will help further research on the involvement of canine CCR7 in LN metastasis. 相似文献
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Akiko Nakashima Munetaka Hosokawa Shigeichi Maeda Susumu Yazawa 《Journal of General Plant Pathology》2007,73(3):225-227
For the first time, Chrysanthemum stunt viroid (CSVd) was detected in commercial dahlia bulbs in Japan. CSVd was found in 77.2% of the tested plants (Dahlia spp.). In nucleotide sequence analysis, a CSVd variant was detected consisting of 354 nucleotides, which differed slightly
from previously reported CSVd variants.
The nucleotide sequence data reported is available in the DDBJ/EMBL/GenBank databases under accession number AB255879. 相似文献
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Inokuma H Yoshizaki Y Matsumoto K Okuda M Onishi T Nakagome K Kosugi R Hirakawa M 《Veterinary parasitology》2004,121(3-4):341-346
A total of 80 free-roaming dogs on Okinawa Island, Japan, were examined for Babesia infection using the polymerase chain reaction (PCR) and sequence analysis. Of 80 samples, 12 were positive in a Babesia genus-specific PCR. Consequent species-specific PCR for B. canis and B. gibsoni revealed that 5 (6.3%) and 7 (8.8%) dogs were infected with B. canis and B. gibsoni, respectively. Sequence analysis of the PCR products revealed that the 18S rRNA gene sequence of B. canis detected from dogs in Okinawa was very close to B. canis vogeli with sequence similarity of 99.94%. 相似文献
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Shun TAKEO Hiroya GOTO Takehito KUWAYAMA Yasunori MONJI Hisataka IWATA 《The Journal of reproduction and development》2013,59(2):174-179
Age-associated deterioration in both the quality and quantity of mitochondria occurs in
older women. The main aim of this study was to examine the effect of age on mitochondrial
DNA copy number (mtDNA number) in early developmental stage bovine embryos as well as the
dynamics of mtDNA number during early embryo development. Real-time PCR was used to
determine mtDNA number. In vitro-produced embryos 48 h after insemination
derived from Japanese black cows, ranging in age from 25 to 209 months were categorized
based on their cleavage status. There was an overall negative relationship between the age
of the cow and cleavage status, to the extent that the ratio of embryos cleaved over the
4-cell stage was greater in younger cows. The mtDNA number did not differ among the
cleaved status of embryos. In the next experiment, oocytes collected from each donor cow
were divided into 2 groups containing 10 oocytes each, in order to compare the mtDNA
number of mature oocytes and early developmental stage embryos within individuals. Upon
comparing the mtDNA number between oocytes at the M2 stage and early developmental stage
48 h post insemination, mtDNA number was found to decrease in most cows, but was found to
increase in some cows. In conclusion, age affects the cleaving ability of oocytes, and
very old cows (> 180 months) tend to have lower mtDNA numbers in their oocytes. The
change in mtDNA number during early development varied among individual cows, although
overall, it showed a tendency to decrease. 相似文献
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Yoshiki HIRATA Yusuke KATSUKURA Yuka HENMI Ren OZAWA Sayaka SHIMAZAKI Akira KUROSAWA Yasushi TORII Hironori TAKAHASHI Hisataka IWATA Takehito KUWAYAMA Koumei SHIRASUNA 《The Journal of reproduction and development》2021,67(4):257
Advanced maternal age is a risk factor for female infertility, and placental dysfunction is considered one of the causes of pregnancy complications. We investigated the effects of advanced maternal aging on pregnancy outcomes and placental senescence. Female pregnant mice were separated into three groups: young (3 months old), middle (8–9 months old), and aged (11–13 months old). Although the body weights of young and middle dams gradually increased during pregnancy, the body weight of aged dams only increased slightly. The placental weight and resorption rate were significantly higher, and live fetal weights were reduced in a maternal age-dependent manner. Although mRNA expression of senescence regulatory factors (p16 and p21) increased in the spleen of aged dams, mRNA expression of p16 did not change and that of p21 was reduced in the placenta of aged dams. Using a cytokine array of proteins extracted from placental tissues, the expression of various types of senescence-associated secretory phenotype (SASP) factors was decreased in aged dams compared with young and middle dams. The aged maternal placenta showed reduced immune cell accumulation compared with the young placenta. Our present results suggest that models using pregnant mice older than 8 months are more suitable for verifying older human pregnancies. These findings suggest that general cellular senescence programs may not be included in the placenta and that placental functions, including SASP production and immune cell accumulation, gradually decrease in a maternal age-dependent manner, resulting in a higher rate of pregnancy complications. 相似文献
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Elly Kesumawati Takushi Kimata Tatsuya Uemachi Munetaka Hosokawa Susumu Yazawa 《Scientia Horticulturae》2006
The interaction between phytoplasma concentration and green-flowering stability was studied in hydrangea cultivars. Three green and 18 nongreen cultivars were subjected to polymerase chain reaction (PCR) analysis to determine Japanese hydrangea phyllody (JHP) phytoplasma infection. The results showed that JHP-phytoplasma was detected only in ‘Midori’ plants, which have green sepals. ‘Midori’ plants were propagated, and from 29 rooted cutting plants, they were grouped into three types on the basis of sepal color, that is, green (75.9%), blue-green (13.8%) and blue (10.3%) sepals. To clarify the variability in the sepal color of ‘Midori’ plants, JHP-phytoplasma concentration in the sepals and leaves of green-, blue-green- and blue-flowering plants was determined by PCR analysis. The semiquantitative PCR comparisons of 370 bp DNA fragments showed that the JHP-phytoplasma concentrations in green sepals were 16 times higher than that in blue-green sepals. JHP-phytoplasma could not be identified by PCR analysis in blue sepals and leaves. These results showed that JHP-phytoplasma concentration correlated with green sepal stability in ‘Midori’ plants. A histological observation of sepals showed that epidermal cells of blue and blue-green sepals had a dome shape. Otherwise, green sepals were leaflike with flat epidermal cells, and palisade parenchyma cells with numerous chloroplasts. 相似文献
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