Clinicopathological Phenotype of Autosomal Recessive Cholesterol Deficiency in Holstein Cattle          下载免费PDF全文

T. Mock  K. Mehinagic  F. Menzi  E. Studer  A. Oevermann  M.H. Stoffel  C. Drögemüller  M. Meylan  N. Regenscheit 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(4):1369-1375

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In vitro effects of bethanechol on specimens of intestinal smooth muscle obtained from the duodenum and jejunum of healthy dairy cows     

Pfeiffer JB  Mevissen M  Steiner A  Portier CJ  Meylan M 《American journal of veterinary research》2007,68(3):313-322

OBJECTIVE: To describe the in vitro effects of bethanechol on contractility of smooth muscle preparations from the small intestines of healthy cows and define the muscarinic receptor subtypes involved in mediating contraction. SAMPLE POPULATION: Tissue samples from the duodenum and jejunum collected immediately after slaughter of 40 healthy cows. PROCEDURES: Cumulative concentration-response curves were determined for the muscarinic receptor agonist bethanechol with or without prior incubation with subtype-specific receptor antagonists in an organ bath. Effects of bethanechol and antagonists and the influence of intestinal location on basal tone, maximal amplitude (A(max)), and area under the curve (AUC) were evaluated. RESULTS: Bethanechol induced a significant, concentration-dependent increase in all preparations and variables. The effect of bethanechol was more pronounced in jejunal than in duodenal samples and in circular than in longitudinal preparations. Significant inhibition of the effects of bethanechol was observed after prior incubation with muscarinic receptor subtype M(3) antagonists (more commonly for basal tone than for A(max) and AUC). The M(2) receptor antagonists partly inhibited the response to bethanechol, especially for basal tone. The M(3) receptor antagonists were generally more potent than the M(2) receptor antagonists. In a protection experiment, an M(3) receptor antagonist was less potent than when used in combination with an M(2) receptor antagonist. Receptor antagonists for M(1) and M(4) did not affect contractility variables. CONCLUSIONS AND CLINICAL RELEVANCE: Bethanechol acting on muscarinic receptor sub-types M(2) and M(3) may be of clinical use as a prokinetic drug for motility disorders of the duodenum and jejunum in dairy cows.  相似文献   

Spontaneous in vitro contractile activity of specimens from the abomasal wall of healthy cows and comparison among dairy breeds     

Zulauf M  Spring C  Eicher R  Meylan M  Hirsbrunner G  Scholtysik G  Steiner A 《American journal of veterinary research》2002,63(12):1687-1694

OBJECTIVE: To characterize and compare in vitro contractility patterns of sections of abomasal wall harvested from cattle of 3 dairy breeds. SAMPLE POPULATION: Longitudinal and circular smooth muscle preparations harvested from the antrum and body of the abomasum of 30 recently slaughtered Holstein-Friesian, Brown Swiss, and Simmental X Red Holstein cows. PROCEDURE: Spontaneous isometric contractions of specimens in tissue baths of modified Krebs solution were recorded during a 4-hour period. Maximal amplitude, frequency of contractions, and change of basal tension were used to characterize contractility. Statistical analyses were used to test for differences among time periods, among breeds, between specimen locations, and between fiber orientations. RESULTS: Myoactivity patterns of abomasal smooth muscle preparations are highly variable and differ on the basis of location and fiber orientation. Frequency of contractions differed significantly among time periods for longitudinally oriented specimens with decreasing frequencies of contractions over time. Maximal amplitude of the longitudinally oriented specimens from the antrum increased significantly, whereas maximal amplitude of the circularly oriented specimens from the antrum decreased significantly. Values did not differ significantly among breeds. CONCLUSIONS AND CLINICAL RELEVANCE: Patterns of spontaneous contractility of abomasal wall specimens are not homogeneous. During a 4-hour recording period, maximal amplitude and frequency of contractions of specimens varied significantly with respect to orientation and location; however, spontaneous contractile myoactivity did not differ significantly among breeds. Therefore, breed predisposition for displaced abomasum is not correlated with spontaneous activity of smooth muscle specimens.  相似文献   

Myoelectric activity of the spiral colon in dairy cows     

Meylan M  Eicher R  Röthlisberger J  Steiner A 《American journal of veterinary research》2002,63(1):78-85

OBJECTIVE: To describe myoelectric activity of the spiral colon in healthy cows. ANIMALS: 7 lactating Simmental X Red-Holstein crossbred cows. PROCEDURE: Cows were implanted with 7 pairs of bipolar silver electrodes (4 in the spiral colon and 1 each in the cecum, distal part of the ileum, and proximal loop of the ascending colon [PLAC]). Myo-electric activity was recorded during 4 days for each cow. Patterns were analyzed, using computer-based methods. RESULTS: Myoelectric activity of the spiral colon was closely associated with motility of the ileum and PLAC and showed the typical organization of migrating myoelectric complexes (MMC). The MMC in the bovine spiral colon (bcMMC) had a mean +/- SD duration of 188.6 +/- 30.8 minutes and was divided into 4 phases. Phases I and II lasted 11.3 +/- 1.4 and 159.4 +/- 33.3 minutes, respectively. Phase III (duration, 5.4 +/- 1.2 minutes) was characterized by 5.2 +/- 0.9 regular spindles (35.4 +/- 5.4 seconds) and 1 final elongated spindle (137.2 +/- 56.4 seconds). Phase III most commonly (73.8 +/- 16.1%) was followed by phase IV (duration, 173 +/- 3.6 minutes). Propagation velocity of phase III was 4.4 +/- 0.5 cm/min, and 13.6% of bcMMC were incompletely propagated through the spiral colon. CONCLUSIONS: Myoelectric activity of the bovine spiral colon is composed of a recurring cyclic pattern similar to MMC of the small intestine. Data of colonic myoelectric activity in healthy cows will serve as a basis for studies on cecal dilatation and dislocation in cattle.  相似文献   

The influence of microfibril angle on the longitudinal shrinkage-moisture content relationship   总被引：6，自引：0，他引：6  

B. A. Meylan 《Wood Science and Technology》1972,6(4):293-301

Summary Incremental longitudinal shrinkage has been measured on 44 samples of Pinus radiata at each 5% increment of moisture content from 0 to 25%. The samples range in mean microfibril angle from 10° to 40°. The data is presented in the form of a family of curves, of incremental shrinkage against microfibril angle, for each moisture content. This family of curves compares very closely with those derived theoretically by Barber [1968] and Cave [1972] based on considering the cell wall as a fibre composite of cellulose microfibrils embedded in a matrix which swells on wetting and whose shear modulus is a function of moisture content.  相似文献   

Genetic Abnormalities in a Calf with Congenital Increased Muscular Tonus          下载免费PDF全文

N. Wiedemar  A.‐K. Riedi  V. Jagannathan  C. Drögemüller  M. Meylan 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(5):1418-1421

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Furunculosis in Atlantic salmon (Salmo salar L.) is not readily controllable by bacteriophage therapy     

David W. Verner&#x;Jeffreys  Myriam Algoet  Michelle J. Pond  Hardeep K. Virdee  Nicola J. Bagwell  Edward G. Roberts 《Aquaculture (Amsterdam, Netherlands)》2007,270(1-4):475-484

The potential of bacteriophage therapy to control bacterial disease in farmed fish was tested using, as an example, furunculosis of Atlantic salmon, caused by Aeromonas salmonicida subsp. salmonicida.

In vivo testing with Atlantic salmon and rainbow trout (Oncorhynchus mykiss Walbaum) showed no adverse effects, with bacteriophage generally cleared within 96 h of administration by either intraperitoneal (i.p.) injection or oral in-feed.

Juvenile Atlantic salmon were administered a combination of bacteriophage O, R and B (1.9 × 10⁸ pfu fish^− 1) by i.p. injection, after they had been challenged with A. salmonicida subsp. salmonicida 78027, also by i.p. injection. The fish that were injected with bacteriophage immediately after challenge died at a significantly slower rate then those that were either not treated with bacteriophage, or treated 24 h post-challenge. However, the end result (100% mortality) was not affected.

In further experiments the effects of oral (1.88 × 10⁵ pfu g^− 1 fish^− 1 daily for 30 days), bath (1.04 × 10⁵ ml^− 1 daily for 30 days) and i.p. (6.25 × 10⁷ pfu fish^− 1) phage treatment to control furunculosis in experimentally infected Atlantic salmon were compared with antibiotherapy (treatment with 10 mg kg^− 1 bw^− 1 day^− 1 oxolinic acid for 10 days), using an indirect cohabitation challenge. No protection was offered by any of the bacteriophage treatments, compared to the positive challenge group, although significant protection was offered by the oxolinic acid treatment. Analysis of samples taken from the trials demonstrated that bacteriophage were correctly administered to the fish and, on occasion, were isolated from fish that had succumbed to furunculosis. It was also shown that bacteriophage resistant A. salmonicida subsp. salmonicida isolates could be recovered from mortalities in all the treatment groups.

The results suggest that, although there were no safety problems associated with the approach, furunculosis in Atlantic salmon is not readily controllable by application of bacteriophage.  相似文献   

Comparison of fecal culture and F57 real-time polymerase chain reaction for the detection of Mycobacterium avium subspecies paratuberculosis in Swiss cattle herds with a history of paratuberculosis     

Selina M Keller  Roger Stephan  Rahel Kuenzler  Mireille Meylan  Max M Wittenbrink 《Acta veterinaria Scandinavica》2014,56(1)

Background

Bovine paratuberculosis is an incurable chronic granulomatous enteritis caused by Mycobacterium avium subspecies paratuberculosis (MAP). The prevalence of MAP in the Swiss cattle population is hard to estimate, since only a few cases of clinical paratuberculosis are reported to the Swiss Federal Food Safety and Veterinary Office each year.Fecal samples from 1,339 cattle (855 animals from 12 dairy herds, 484 animals from 11 suckling cow herds, all herds with a history of sporadic paratuberculosis) were investigated by culture and real-time polymerase chain reaction (PCR) for shedding of MAP.

Results

By culture, MAP was detected in 62 of 445 fecal pools (13.9%), whereas PCR detected MAP in 9 of 445 pools (2.0%). All 186 samples of the 62 culture-positive pools were reanalyzed individually. By culture, MAP was grown from 59 individual samples (31.7%), whereas PCR detected MAP in 12 individual samples (6.5%), all of which came from animals showing symptoms of paratuberculosis during the study. Overall, MAP was detected in 10 out of 12 dairy herds (83.3%) and in 8 out of 11 suckling cow herds (72.7%).

Conclusions

There is a serious clinically inapparent MAP reservoir in the Swiss cattle population. PCR cannot replace culture to identify individual MAP shedders but is suitable to identify MAP-infected herds, given that the amount of MAP shed in feces is increasing in diseased animals or in animals in the phase of transition to clinical disease.  相似文献   

Ectomycorrhizal communities in a Mediterranean forest ecosystem dominated by <Emphasis Type="Italic">Quercus ilex</Emphasis>: seasonal dynamics and response to drought in the surface organic horizon     

Franck Richard  Melanie Roy  Oula Shahin  Christopher Sthultz  Myriam Duchemin  Richard Joffre  Marc-André Selosse 《Annals of Forest Science》2011,68(1):57-68

• Introduction   

Millions of hectares of Quercus ilex forests dominate disturbed landscapes in the western part of the Mediterranean basin. Although these forests are very widespread, little is known about the composition and structure of their associated ectomycorrhizal fungal communities.  相似文献   

Diversity in phosphorus mobilisation and uptake in ectomycorrhizal fungi     

Claude Plassard  Julien Louche  Muhammad A. Ali  Myriam Duchemin  Elvira Legname  Benoît Cloutier-Hurteau 《Annals of Forest Science》2011,68(1):33-43

• Introduction   

Phosphorus (P) is often the first or second element limiting aboveground net primary productivity of forests. Besides low available inorganic orthophosphate (Pi) concentrations, soil may contain high total P contents, as insoluble mineral P or as organic P. Most plants form mycorrhizal associations that improve their P nutrition. Three main hypotheses have been proposed to explain this positive effect through an increase of (1) P mobilisation from mineral P, (2) P mobilisation from organic P and (3) soil exploration and P uptake. However, the positive effect of mycorrhizal symbiosis may be variable with the fungal species forming the association. This could be due to the different abilities of mycorrhizal fungi to mobilise P and/or to take up Pi from the soil.  相似文献