Nephrotoxicity and oxidative stress of single large dose or two divided doses of gentamicin in rats     

Kandeel M  Abdelaziz I  Elhabashy N  Hegazy H  Tolba Y 《Pakistan journal of biological sciences: PJBS》2011,14(11):627-633

Gentamicin (GS) is a potent antimicrobial exhibiting concentration dependent bacterial killing. A high dose ofgentamicin (10 mg kg(-1)) is required to reach sufficient concentrations in specific fluids as cerebrospinal fluid and to be effective on antibiotics resistant bacteria as well as treatment of acute and dangerous illness. Using a rat model, the renal toxicity and oxidative stress of administering gentamicin (10 mg kg(-1) daily for 7 days) either in a single dose or divided into 2 doses was investigated. The safety of dose regimens was assessed through oxidant-antioxidant parameters as well as renal function tests. Typical renal damage and high oxidative stress were evident in the control group receiving 100 mg kg(-1) gentamicin daily for 7 days. This was verified by high serum urea, uric acid, creatinine as well as increase in the levels of oxidative stress biomarkers as malondialdehyde, NO, total antioxidant capacity and decrease in reduced glutathione level. At any of the used regimen, 10 mg kg(-1) gentamicin did not provide high compromise for renal functions nor significantly increased the oxidative stress and tissue damage. Based on microscopic lesions scores and biochemical analysis, there were no significant differences between single or two divided dosages of gentamicin at dose rate of 10 mg kg(-1) day(-1). Further studies are required for applications in other animals of human subjects.  相似文献   

Microbiological study and insecticidal potential of purified extract from Streptomyces sp. on the larvae of Galleria mellonella     

Soliman  Mahmoud Abdel Wahab  Hamza  Ali Fawzy  Zahran  Nagwan Fahmy  Bassioni  Ghada 《植物病害和植物保护杂志》2021,128(6):1565-1574

Journal of Plant Diseases and Protection - In the present study, one Streptomyces species was isolated from a soil sample that was collected in Khafr El Shikh Governorate. Actinomycetes are...  相似文献   

Repair and strengthening of timber cantilever beams     

Alaa A. Bashandy  Alaa E. El-Habashi  Ahmed K. Dewedar 《Wood material science & engineering》2018,13(4):241-253

This research aims to study the feasibility of repair and strengthening of timber cantilever beams used in historic buildings. It is conducted to investigate the feasibility of using different valid materials and techniques to repair and strengthen timber cantilever beams in new and historic timber buildings. The study is performed in terms of structural performance as well as historic and architectural values. An experimental program of several different materials and techniques is executed. The results are driven in terms of initial cracking load, crack propagation for the tested samples at different loading stages, deflection values, and failure load for each repaired or strengthened material and technique. The main variables are timber types (new and historic), repair and strengthening materials (steel plates, Glass Fiber Reinforced Polymer wrap, and Carbon Fiber Reinforced Polymer laminates), repair or strengthening techniques (near surface mounted and externally bonded system), and cantilever length (1000 and 1250?mm). Test results indicated that using steel plates, glass fiber wrap, and carbon fiber laminates increases the value of failure loads and decreases the deflection at both; repaired and strengthened timber cantilever beams compared to control beams.  相似文献   

Neuropathogenicity of Equine Herpesvirus 9 in Cattle     

Nagwan El-Habashi  El-Shaymaa El-Nahass  Yasuji Namihira  Hiroko Hagiwara  Hideto Fukushi  Minoru Narita  Akihiro Hirata  Hiroki Sakai  Tokuma Yanai 《Journal of Equine Veterinary Science》2011,31(2):72-77

The pathogenicity of equine herpesvirus 9 (EHV-9), a neurotropic equine herpesvirus isolated from a herd of Gazella thomsoni, was studied in cattle. Seven calves were inoculated intranasally with 10⁵ and 10⁷ plaque-forming units of the EHV-9 P19. Three animals showed brain lesions consisting of glial reactions and perivascular cuffings in the olfactory bulb and the frontal and temporal lobes. Additionally, the animal that was inoculated with 10⁷ plaque-forming units showed neuronal degeneration and loss, as well as nuclear inclusions compatible with herpesvirus. EHV-9 was isolated from the brain of calf 6 and the lungs of calves 1 and 2. The results suggested that cattle are susceptible to experimental infection with EHV-9 and at risk from natural infection from reservoir hosts.  相似文献   

Effect of using Spirulina and Chlorella as feed additives for elevating immunity status of Nile tilapia experimentally infected with Aeromonas hydrophila     

Nagwan El‐Habashi  Sabreen E. Fadl  Hanaa F. Farag  Doaa M. Gad  Abdelgawad Y. Elsadany  Mohamed S. El Gohary 《Aquaculture Research》2019,50(10):2769-2781

The present trial was performed to evaluate the effect of dietary incorporation of dried Spirulina, Chlorella and their mixture on the immune status of Nile tilapia (Oreochromis niloticus) before and after artificial infection with pathogenic bacterium Aeromonas hydrophila (A. hydrophila). Two hundred and forty fish were divided into four groups: (a) a control group fed on a basal diet only, (b) a second group fed on a diet containing Spirulina 15%, (c) a third group fed on a diet containing Chlorella 15%, and (d) a fourth group fed on a diet containing a mixture of both Spirulina 15% and Chlorella 15%. At the end of the experiment (9 weeks), the four groups were experimentally infected with A. hydrophila for 7 days. Antioxidant enzymes, lysozyme and bactericidal activities and histopathological changes were determined just before the challenge test and 7 days post‐challenge. Significant (p ≤ 0.05) increases in fish body protein% before the challenge test and increases in serum antioxidant enzymes, lysozyme and bactericidal activity in the Chlorella and algal mixture groups before and after the challenge test were observed. Spirulina, Chlorella and their mixture groups significantly decreased serum malondialdehyde compared to the control group before and after the challenge test. Using Spirulina, Chlorella and their mixture mitigated the necrotic and degenerative changes induced by A. hydrophila and revealed well‐developed and multiple melanomacrophage centres. Thus, dietary Spirulina, Chlorella and their mixture inclusion in Nile tilapia fish proved to have a protective effect against A. hydrophila infection.  相似文献