Effects of species and sex on plasma hormone and metabolite concentrations in crossbred Brahman cattle and crossbred water buffalo     

T. Ban-Tokuda  E.A. Orden  A.N. Barrio  R.M. Lapitan  C. Delavaud  Y. Chilliard  T. Fujihara  L.C. Cruz  H. Homma  Y. Kanai 《Livestock Science》2007,107(2-3):244-252

Buffalo meat is tasty and indistinguishable from beef, and has been described as being lean and low in cholesterol in comparison with beef. However, little is known about the plasma hormone and metabolic profiles related to fat metabolism in buffalo, and how their levels are affected by species and sex. In this study levels of plasma hormones and metabolites were compared between species and sexes. Ten growing crossbred Brahman cattle and ten growing crossbred buffaloes (five males and five females in each), with an average age of 22 months were used. The animals were fed on an experimental diet composed of corn silage, brewer's spent grain and a concentrate mixture at a 50:30:20 ratio on a dry matter basis during a fattening period of 6 months. Blood samples were collected every month during the experiment, and were analysed for plasma leptin, insulin, glucose, triglyceride (TG) and total cholesterol (TC) concentrations. Plasma leptin, insulin and TC concentrations increased during fattening and were significantly (P < 0.05) higher in cattle than in buffaloes. These concentrations in cattle showed a big difference between sexes in comparison with buffaloes, and were higher in females than in males. The plasma glucose and TG concentrations did not differ (P > 0.05) between cattle and buffaloes.

The crude fat content of longissimus muscle was significantly higher in cattle than in buffalo. Differences in body fat deposition seem to have an influence on plasma leptin, insulin and TC concentrations differently between cattle and buffaloes.  相似文献   

Permuted tRNA genes expressed via a circular RNA intermediate in Cyanidioschyzon merolae     

Soma A  Onodera A  Sugahara J  Kanai A  Yachie N  Tomita M  Kawamura F  Sekine Y 《Science (New York, N.Y.)》2007,318(5849):450-453

A computational analysis of the nuclear genome of a red alga, Cyanidioschyzon merolae, identified 11 transfer RNA (tRNA) genes in which the 3' half of the tRNA lies upstream of the 5' half in the genome. We verified that these genes are expressed and produce mature tRNAs that are aminoacylated. Analysis of tRNA-processing intermediates for these genes indicates an unusual processing pathway in which the termini of the tRNA precursor are ligated, resulting in formation of a characteristic circular RNA intermediate that is then processed at the acceptor stem to generate the correct termini.  相似文献   

Induction of antinuclear antibodies in rabbits by infection with rinderpest viruses with different degree of virulence     

K Imaoka  Y Kanai  Y Nishikawa  Y Yoshikawa  K Yamanouchi 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1988,50(5):1115-1118

  相似文献   

Lectin-binding patterns in the spermatogenic cells of the shiba goat testis.   总被引：1，自引：0，他引：1  

M Kurohmaru  Y Kanai  Y Hayashi 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(5):893-897

Lectin-binding patterns in the testis of the sexually mature goat were investigated by light and transmission electron microscopy. Dolichos biflorus agglutinin (DBA) and Griffonia simplicifolia agglutinin-I (GS-I) were negative in the seminiferous epithelium, but soybean (Glycine max) agglutinin (SBA), Griffonia simplicifolia agglutinin-II (GS-II) and peanut (Arachis hypogaea) agglutinin (PNA) were positive in the acrosomal vesicle of the Golgi-phase spermatids and in the acrosome of the cap-, acrosome- and maturation-phase spermatids. In addition, PNA was positive also in the plasma membrane and cytoplasm of the spermatogenic cells from the late pachytene spermatocytes to the maturation-phase spermatids. This finding indicates that glycoconjugates containing D-galactose residue appear at this period. Therefore, PNA may be a useful marker of developing spermatogenic cells. Electron microscopically, the positive reactions of SBA, GS-II and PNA were demonstrated in the central dense area (acrosomal granule) of the cap-phase spermatid acrosome, but not in the peripheral low-dense area. These results indicate that the lectin-bindings in the goat testis not only are common features, but also have patterns somewhat different from those in other mammals in such a way that GS-II can be detected in the acrosomal region of all phases of spermatids and that PNA is positive in the late spermatid acrosome.  相似文献   

Effects of heat stress on the redox status in the oviduct and early embryonic development in mice     

Matsuzuka T  Ozawa M  Nakamura A  Ushitani A  Hirabayashi M  Kanai Y 《The Journal of reproduction and development》2005,51(2):281-287

This study examined the association between redox status in the oviduct and early embryonic death in heat-stressed mice. In Experiment 1, non-pregnant mice were heat-stressed at 35 C with 60% relative humidity for 12, 24, or 36 h, and the maternal redox status was verified by measuring the levels of reactive oxygen species (ROS) and free radical scavenging activity (FRSA) in the oviduct, and thiobarbituric acid reactive substances (TBARS) and glutathione peroxidase (GSH-Px) activity in the liver. In Experiment 2, zygotes were collected from mice heat-stressed for 12 h on the day of pregnancy, and their developmental abilities were assessed in vitro, along with the intensity of DNA damage at the 2-cell stage. The TBARS value and GSH-Px activity in the liver, and ROS level in the oviduct were significantly higher in heat-stressed mice, and this increase appeared to depend on the duration of the heat stress. Maternal heat stress significantly reduced the percentage of zygotes that developed to the morula and blastocyst and the total cell number in the blastocyst. In addition, DNA damage at the 2-cell stage was significantly higher in maternally heat-stressed embryos. These results suggest that heat stress induces systemic changes in redox status in the maternal body, and the resultant increase in oxidative stress in the oviduct is possibly involved in heat stress-induced early embryonic death .  相似文献   

An ultrastructural study on the Leydig and Sertoli cells in the immature lesser mouse deer (Tragulus javanicus)     

Andriana BB  Kanai Y  Kimura J  Fukuta K  Hayashi Y  Kurohmaru M 《Anatomia, histologia, embryologia》2005,34(3):171-175

Leydig and Sertoli cells of the immature lesser mouse deer testes, obtained in East Malaysia, were observed using light and transmission electron microscopy (TEM). The testes were fixed in 5% glutaraldehyde, post-fixed in 1% OsO4, dehydrated in ethanol, and embedded in Araldite M. Serial semi-thin sections were cut, stained with toluidine blue and observed using light microscopy. Serial ultra-thin sections were cut, stained with uranyl acetate and lead citrate, and examined using TEM. As a result, ultrastructurally, two types of underdeveloped filament bundles were infrequently recognized in Leydig cells, but not in other testicular cells. One type was the underdeveloped bundles of actin filaments (approximately 5 nm in diameter), which were found in the nucleus of Leydig cells. The other type was the underdeveloped bundles of intermediate filaments (approximately 10 nm in diameter), which were found in the cytoplasm of Leydig cells. A multivesicular nuclear body (MNB)--specifically present in the Sertoli cell nucleus of ruminant testes--was infrequently observed. The MNB is situated in the vicinity of nuclear membrane, still in an underdeveloped stage.  相似文献   

Preferential expression of L-type amino acid transporter 1 in ameloblasts during rat tooth development     

Park JC  Kim YB  Yoon JH  Kim HJ  Kim SM  Kanai Y  Endou H  Kim DK 《Anatomia, histologia, embryologia》2004,33(2):119-124

Certain amino acid transport systems play an important role in supplying organic nutrients to each cell and for cell proliferation during tooth development. However, the mechanisms responsible for such actions are unclear. This study demonstrated for the first time that LAT1 and 4F2hc are expressed during tooth development in prenatal and postnatal rats, and that the transporters show cell-specific expression in ameloblasts, which are the epithelium-derived dental cells. LAT1 and 4F2hc expression was not observed in other dental cells of the developing teeth such as odontoblasts and cementoblasts. Overall, these results suggest that LAT1 and 4F2hc might play an important role in enamel formation.  相似文献   

Effects of trichostatin a, a histone deacetylase inhibitor, on mouse gonadal development in vitro     

Mizukami T  Fujisawa M  Kanai Y  Kurohmaru M  Hayashi Y 《The Journal of reproduction and development》2004,50(2):227-235

  相似文献   

Myocardial injury-related changes in plasma NT-proBNP and ANP concentrations in a canine model of ischemic myocardial injury     

Hori Y  Ohshima N  Chikazawa S  Kanai K  Hoshi F  Itoh N  Higuchi S 《Veterinary journal (London, England : 1997)》2012,191(1):46-51

Serial changes in plasma N-terminal pro-B-type natriuretic peptide (NT-proBNP) and atrial natriuretic peptide (ANP) concentrations are unknown in dogs with myocardial injury. The time-course secretory responses between NT-proBNP and ANP or cardiac troponin-T (cTnT) related to myocardial infarction (MI) were investigated in this study. Six dogs were anaesthetised and the left anterior descending artery was ligated. A transient decrease in cardiac function was detected 1h after MI but returned to baseline levels within 7 days and remained so for 6 months. Echocardiographic examination revealed focal ventricular dyskinesis throughout the study. Six months following MI, the left atrium to aorta ratio increased significantly although the relative wall thickness decreased significantly from baseline. Significantly elevated plasma NT-proBNP and cTnT concentrations were detected 1 day after MI and these gradually decreased over 28 days to baseline levels without left ventricular pressure elevation. Plasma ANP was elevated significantly 6 months after MI. The NT-proBNP assay is a helpful diagnostic indicator for identifying asymptomatic acute and subacute myocardial injury whereas plasma ANP concentration mainly reflects atrial dilation.  相似文献   

Conditioning of karyoplasts for producing somatic nuclear transferred gonadal germ cells in domestic chickens     

Kohara Y  Minematsu T  Aikawa T  Kanai Y  Tajima A 《The Journal of reproduction and development》2008,54(3):221-224

The objective of this study was to establish a protocol for generating karyoplasts that can be used to produce somatic nuclear transferred gonadal germ cells (snt-GGCs) in domestic chickens. Karyoplasts were produced by centrifuging cultured fibroblasts from 10-day-old chick embryos at 10,000 x g in the presence of 1.0 microg/ml cytochalasin B. The number of karyoplasts was significantly (P<0.05) higher and the diameters of the karyoplasts were significantly (P<0.05) smaller when fibroblasts were centrifuged for 60 min than for 10 or 30 min. It was possible to generate snt-GGCs by electrofusion of GGCs with karyoplasts produced from cryopreserved or serum-starved fibroblasts. These results indicate that karyoplasts generated from 10-day-old chick embryos can be used to produce snt-GGCs even after cryopreservation and serum starvation of the fibroblasts.  相似文献