The Effect of Exogenous Hormone Treatment on Spermiation in Rhynchocypris oxycephalus (Sauvage and Dabry)     

I.-S. Park  G. C. Choi  D. S. Kim  Y. K. Nam 《Journal of the World Aquaculture Society》2002,33(4):494-500

For the evaluation of hormonal control of spermiation in fish, a method to quanify the spermiation response of mature Rhynchocypris oxycephalus (Sauvage and Dabry) to hormonal therapy is described. Spermatocrit was determined after 7 min centrifugation at 18,000 ± g and sperm density was estimated by a standard hemocytomer method. Sperm density can be predicted from spermatocrit since their relationship is linear as described by the regression equation, Y = 3.68X - 27.18 ( R ² = 0.82, N = 50). where Y is spermatocrit and × is sperm density. Milt production by mature R. oxycephalus was highest at 24 h after injection of 1,000 IU human chorionic gonadotropin (HCG) and 50 μg luteinizing hormone-releasing hormone analogue (LHRHa) per kg body weight. Increased milt production coincided with low spermatocrit and sperm density levels. These results demonstrate that spermiation in mature R. oxycephalus can be reliably evaluated by a spermatocrit method and that HCG and LHRHa are effective in stimulating of spermiation in this species.  相似文献   

Differential expression of 3beta-hydroxysteroid dehydrogenase mRNA in rat testes exposed to endocrine disruptors     

Kim HH  Kwak DH  Yon JM  Baek IJ  Lee SR  Lee JE  Nahm SS  Jeong JH  Lee BJ  Yun YW  Nam SY 《The Journal of reproduction and development》2007,53(3):465-471

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Expression pattern of sulfated glycoprotein-2 (SGP-2) mRNA in rat testes exposed to endocrine disruptors   总被引：1，自引：0，他引：1  

Yon JM  Kwak DH  Cho YK  Lee SR  Jin Y  Baek IJ  Lee JE  Nahm SS  Choo YK  Lee BJ  Yun YW  Nam SY 《The Journal of reproduction and development》2007,53(5):1007-1013

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Identification of embryonic stem cell activities in an embryonic cell line derived from marine medaka (Oryzias dancena)     

Dongwook Lee  Jun Hyung Ryu  Seung Tae Lee  Yoon Kwon Nam  Dong Soo Kim  Seung Pyo Gong 《Fish physiology and biochemistry》2015,41(6):1569-1576

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Superficial digital flexor tendonitis in Thoroughbred race horses: outcome following non-surgical treatment and superior check desmotomy     

KT GIBSON  HM BURBIDGE  DU PFEIFFER 《Australian veterinary journal》1997,75(9):631-635

Objective This study documents the results of non-surgical treatment and treatment by superior check desmotomy in Thoroughbred racehorses with superficial digital flexor (SDF) tendonitis. Design A prospective study was made of 124 thoroughbred racehorses with unilateral or bilateral SDF tendonitis. Procedure The flexor tendons were assessed by physical and ultrasonographic examination before treatment, and the lesions detected in affected tendons were characterised according to lesion type, length and cross-sectional area. Ninety three horses were managed non-surgically and 31 by superior check desmotomy. Recurrent or new injuries were defined as injuries affecting a previously injured superficial digital flexor tendon, the contralateral SDF tendon, or the suspensory ligament (interosseous muscle) in either forelimb. Results No statistically significant difference was found in ultrasonographic lesion severity between treatment groups. Horses managed by superior check desmotomy were 1.3 times more likely to complete five or more races than horses managed non-surgically (95% confidence limits 0.93–1.82). Horses treated surgically were 1.2 times more likely to develop recurrent or new injuries after returning to training than horses managed non-surgically (95% CL 0.95–1.55). Horses under-going superior check desmotomy were 5.5 times more likely to develop suspensory desmitis than horses treated non-surgically (95% CL 1.13–26.4). There was no difference in the time to recurrent or new injury between treatment groups. Conclusion There was no statistically significant difference between treatment groups in the proportions of horses able to complete five or more races after an episode of superficial digital flexor tendonitis. Superior check desmotomy did not appear to offer an advantage over non-surgical treatment in preventing recurrent or new injuries in Thoroughbred racehorses. Horses undergoing superior check desmotomy appeared to be at greater risk of developing suspensery ligament injuries than horses managed non-surgically.  相似文献   

Comparison of the canine corneal epithelial cell sheets cultivated from limbal stem cells on canine amniotic membrane,atelocollagen gel,and temperature‐responsive culture dish          下载免费PDF全文

Eunryel Nam  Naoki Fujita  Maresuke Morita  Keiko Tsuzuki  Hsing Yi Lin  Cheng Shu Chung  Takayuki Nakagawa  Ryohei Nishimura 《Veterinary ophthalmology》2015,18(4):317-325

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Isolation of two anti-inflammatory and one pro-inflammatory polyunsaturated fatty acids from the brown seaweed Undaria pinnatifida     

Khan MN  Cho JY  Lee MC  Kang JY  Park NG  Fujii H  Hong YK 《Journal of agricultural and food chemistry》2007,55(17):6984-6988

Two anti-inflammatory omega-3 polyunsaturated fatty acids (PUFAs) of stearidonic acid (SA) and eicosapentaenoic acid (EPA) and one pro-inflammatory omega-6 PUFA of arachidonic acid (AA) were isolated from the edible brown seaweed Undaria pinnatifida. SA was active against mouse ear inflammation induced by phorbol myristate acetate, with IC50 values of 160, 314, and 235 microg per ear for edema, erythema, and blood flow, respectively. EPA was also active against edema, erythema, and blood flow, with IC50 values of 230, 462, and 236 microg per ear, respectively. Although AA at low concentrations showed anti-inflammatory activities when measured 10 h later, AA doses of more than 243 microg per ear induced inflammatory symptoms 1 h later. Mature thalli generally had larger amounts of PUFAs than young thalli. The algal blade contained more omega-3 PUFAs than were found in other parts, while the holdfast contained extremely high amounts of AA. Late-season thalli showed increased amounts of PUFAs, especially AA.  相似文献   

Survival rate and growth performance of Cyprinus carpio L. in intensively cultivated rice fields     

N Vromant  C Q Nam  N T H Chau  & F Ollevier 《Aquaculture Research》2004,35(2):171-177

In the freshwater area of Vietnam's Mekong Delta, Barbodes gonionotus (Bleeker), Cyprinus carpio L. and Oreochromis niloticus (L.) are often reared together in rice fields. The survival rate and specific growth rate (SGR) of C. carpio showed a large variance. To understand the underlying reasons for this variation, we used multiple regressions for the variables SGR and survival rate, using the results of eight experiments in such polycultures at the Co Do rice–fish experimental station. The SGR of C. carpio declined with increasing rice density. Interspecific competition with O. niloticus also had a negative effect on C. carpio SGR. When fertilizer or manure is added to the trench of the rice–fish field or when extra food can be given to the fish, O. niloticus seems more appropriate than C. carpio. The survival rate of C. carpio depended on season, C. carpio stocking weight and density and the biomass of wild fish species. To obtain higher survival rates, one should stock fewer fish but of a larger size, after thorough eradication of wild fish species.  相似文献   

Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment     

Dae Young Yoo  Woosuk Kim  Dae Won Kim  Sung Min Nam  Hyo Young Jung  Jong Whi Kim  Choong Hyun Lee  Jung Hoon Choi  Moo-Ho Won  Yeo Sung Yoon  In Koo Hwang 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(1):27-33

In this study, we determined how rosiglitazone (RSG) differentially affected hippocampal neurogenesis in mice fed a low-fat diet (LFD) or high-fat diet (HFD; 60% fat). LFD and HFD were given to the mice for 8 weeks. Four weeks after initiating the LFD and HFD feeding, vehicle or RSG was administered orally once a day to both groups of mice. We measured cell proliferation and neuroblast differentiation in the subgranular zone of the dentate gyrus using Ki67 and doublecortin (DCX), respectively, as markers. In addition, we monitored the effects of RSG on the levels of DCX and brain-derived neurotrophic factor (BDNF) in hippocampal homogenates. At 8 weeks after the LFD feeding, the numbers of Ki67- and DCX-positive cells as well as hippocampal levels of DCX and BDNF were significantly decreased in the RSG-treated group compared to the vehicle-treated animals. In contrast, the numbers of Ki67- and DCX-positive cells along with hippocampal levels of DCX and BDNF in the HFD fed mice were significantly increased in the RSG-treated mice compared to the vehicle-treated group. Our data demonstrate that RSG can modulate the levels of BDNF, which could play a pivotal role in cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus.  相似文献   

Numerical modeling and design of inflatable structures—application to open-ocean-aquaculture cages     

Jeffrey D. Suhey  Nam H. Kim  Christopher Niezrecki   《Aquacultural Engineering》2005,33(4):285-303

Numerical simulation and design of an inflatable open-ocean-aquaculture cage is presented using nonlinear finite element analysis of membrane structures. Numerical instability caused by the tension-only membrane has been removed by adding an artificial shell with small stiffness. The material properties of a fabric material are obtained from tensile tests in both hoop and longitudinal directions, assuming the material is anisotropic. Wrinkling, defined as an onset of compressive stress, is monitored as design criteria. The finite element model is validated using a modified beam theory for the inflatable structure by comparing the maximum deflection and stress. Good agreement is observed between the numerical and theoretical results. A full-scale cage model is created using membrane, shell, and string elements to test the stiffness and integrity of the system. The feasibility study indicates that the inflated structure has sufficient stiffness to be used as the structural support within a fish cage. Based on the parameter study, several designs are suggested.  相似文献