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1.
Miori Kishimoto Sa-Hun Pae Naoyoshi Muroya Hirokazu Watarai Hiroshi Anzai Ki-Ja Lee Junichiro Shimizu Motoki Sasaki Kazutaka Yamada 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(1):89-91
Caudolateral curvilinear osteophyte (CCO), an osteophyte at the site of joint capsule attachment on the caudal aspect of the femoral neck, has been advocated as a radiographic criterion for coxofemoral subluxation. The correlation between the presence of CCO on radiographs (radiographic-CCO), the size of the CCO (CCO index) on three-dimensional computed tomographic (CT) images, and hip evaluation using transverse CT images was assessed in 22 Border Collies. CCOs were detected on the radiographs and CT images of 32% and 100% femurs, respectively. The CCO index correlated significantly with radiographic-CCO, but a large CCO index did not necessarily imply that the CCO was visible on radiographs. Hence, radiographic-CCO findings should be used cautiously in hip evaluation of Border Collies. 相似文献
2.
Ikadai H Nagai A Xuan X Igarashi I Tsugihiko K Tsuji N Oyamada T Suzuki N Fujisaki K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(4):325-328
Antibodies to Babesia caballi and Babesia equi were examined on a total of 2,019 horse serum samples that had been collected in 1971-1973 by the National Institute of Animal Health by enzyme-linked immunosorbent assay (ELISA) using recombinant proteins and by Western-blot analysis. Based on the criterion for positivity by ELISA, 5.4% (109/2,019) and 2.2% (44/2,019) had antibodies against B. caballi and B. equi, respectively. The ELISA-positive sera were further examined by Western blot; 30/109 for B. caballi and 2/ 44 for B. equi were positive for native B. caballi or B. equi, but none of them was seropositive for both infections. Based on the results of this study, further investigations should be required to survey horses that have arrived in Japan relatively recently and tick vectors of equine Babesia using ELISA with some recombinant protein, a parasite detection method in an in vitro culture of equine Babesia, and PCR testing. 相似文献
3.
Masaki Okuda Isao Aramaki Takuya Koseki Naoyoshi Inouchi Katsumi Hashizume 《Cereal Chemistry》2006,83(2):143-151
Structural and physicochemical characteristics of endosperm starch from milled rice grains of seven Japanese cultivars used in sake production were examined. Amylose content was 15.2–20.2%, number-average degree of polymerization (DPn) of amylose was 900–1,400, and the ratio of short-to-long chain amylopectin was 2.7–3.5, respectively. The degree of retrogradation of purified starch stored for seven days at 4°C after gelatinization was 20–31%. The degree of retrogradation correlated negatively with the ratio of short-to-long chain amylopectin. The effect of holding time after steaming on enzyme digestibility and starch retrogradation of steamed rice grains was investigated. The longer the holding time after steaming, the greater the extent of retrogradation, and the less the degree of enzymatic digestibility. The decreased rate of enzyme digestibility correlated with amylopectin chain length distribution. Samples with short-chain amylopectin exhibited a slow decrease in enzyme digestibility. It was determined that the structure and retrogradation properties of endosperm starch in Japanese rice cultivars affect the decreasing rate of enzyme digestibility of the steamed, milled rice grains. 相似文献
4.
Yi Zhang Kounosuke Otomaru Kazunaga Oshima Yuji Goto Ichiro Oshima Susumu Muroya Mitsue Sano Rena Saneshima Yukiko Nagao Aoi Kinoshita Yasuko Okamura Sanggun Roh Akira Ohtsuka Takafumi Gotoh 《Animal Science Journal》2021,92(1):e13600
This study aimed to investigate the effects of high and low levels of energy intake during the entire gestation period on the skeletal muscle development, organ development, and adipose tissue accumulation in fetuses of Wagyu (Japanese Black) cows, a breed with highly marbled beef. Cows were allocated to a high-nutrition (n = 6) group (fed 120% of the nutritional requirement) or low-nutrition (n = 6) group (fed 60% of the nutritional requirement). The cows were artificially inseminated with semen from the same sire, and the fetuses were removed by cesarean section at 260 ± 8.3 days of fetal age and slaughtered. The whole-body, total muscle, adipose, and bone masses of the fetal half-carcasses were significantly higher in the high-nutrition group than the low-nutrition group (p = 0.0018, 0.009, 0.0004, and 0.0362, respectively). Fifteen of 20 individual muscles, five of six fat depots, nine of 17 organs, and seven of 12 bones that were investigated had significantly higher masses in the high-nutrition group than the low-nutrition group. The crude components and amino acid composition of the longissimus muscle significantly differed between the low- and high-nutrition groups. These data indicate that maternal nutrition during gestation has a marked effect on the muscle, bone, and adipose tissue development of Wagyu cattle fetuses. 相似文献
5.
The starches were separated from unripe apples of five cultivars (Criterion, Ruspippum, Red Spur, Skyline Supreme, and Granny Smith) and evaluated using scanning electron microscopy (SEM), gel permeation chromatography (GPC), X-ray diffraction, differential scanning calorimetry (DSC), and dynamic viscoelasticity. SEM showed the presence of round granules as well as granules that had been partially degraded, probably by amylases. The starch granules in different apple starches ranged between 4.1 and 12.0 mum. Debranching of starch with isoamylase and subsequent fractionation of debranched materials by GPC revealed the presence of an apparent amylose, an intermediate fraction (mixture of amylose and amylopectin), long side chains of amylopectin, and short side chains of amylopectin in the range of 28-35.2, 3.6-4.4, 20-21.3, and 39.9-47.1%, respectively. The swelling power of starches ranged between 14.4 and 21.3 g/g. X-ray diffraction of apple starches showed a mixture of A- and B-type patterns. All apple starches showed peak intensities lower than that observed for normal corn and potato starch, indicating the lower crystallinity. The transition temperatures (onset temperature, T(o); peak temperature, T(p); and conclusion temperature, T(c)) and enthalpy of gelatinization (deltaH(gel)) determined using DSC ranged between 54.7 and 56.2 degrees C, between 57.1 and 59.1 degrees C, between 60.2 and 63.5 degrees C, and between 3.3 and 4.2 J/g, respectively. The viscoelastic properties of starch from different cultivars measured during heating and cooling using a rheometer differed significantly. Red Spur and Criterion starches with larger granule size showed higher G' and G' ' values, whereas those containing smaller size and amylolytically degraded granules showed lower G' and G' '. 相似文献
6.
Kitamura S Muroya S Tanabe S Okumura T Chikuni K Nishimura T 《Journal of agricultural and food chemistry》2005,53(10):4178-4181
Troponin T (TnT) is one of the myofibrillar proteins that is easily degraded during postmortem aging of pork. In this study, we determined the N-terminal amino acid sequences of TnT degradation fragments produced during postmortem aging and by m-calpain hydrolysis. The N-terminal amino acid sequences of TnT fragments produced during postmortem aging were EVHEPEEKPRPKLTAP, EKPRPKLTAPKIPEG, and APKIPEGEKVDF. On the other hand, the N-terminal amino acid sequences of TnT fragments produced by the action of m-calpain were APPPPAEV, EVHEPEEK, and APK. These sequences of degradation fragments could be mapped on fast type TnT isoform 2. The peptide bonds of His37-Glu38 and Thr51-Ala52 in fTnT2 were cleaved during postmortem aging as well as by the calpain hydrolysis; therefore, calpain was concluded to have an important role in TnT degradation during postmortem aging. It was also found that the sourness-suppressing peptide APPPPAEVHEVHEEVH (Okumura et al. Biosci. Biotechnol. Biochem. 2004, 68, 1657-1662) derived from TnT degradation could be produced by the action of calpains on Glu21-Ala22 and His37-Glu38 sites. 相似文献
7.
The effect of soil fauna-mediated leaf litter (faecal pellets) versus mechanically fragmented (finely ground) leaf litter on biomass production of rice (Oryza sativa, var. Primavera) was assessed in pot tests. Rice seedlings were either grown in soil samples amended with faecal pellets of diplopods and isopods fed on leaf litter of a legume cover crop (Pueraria phaseoloides (Roxb.) Benth) and a peach palm (Bactris gasipaes) or in soil amended with finely ground leaf litter. The addition of faecal pellets caused a significant and dose-related increase in plant biomass compared to pure soil. Ground leaf litter induced a significantly smaller positive effect on plant biomass development with Pueraria litter > Bactris litter > mixed primary forest litter. In contrast, soil microbial biomass development during the 4 weeks plant test was higher in the soil amended with ground litter as compared to soil amended with feacal pellets. The results show a clear positive effect of the soil fauna on soil fertility and indicate differences in the availability of nutrients from the organic substrates to higher plants and soil microorganisms. 相似文献
8.
Maeda N Inoshima Y Oouchi S Uede T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(11):1493-1495
Surveillance of jaagsiekte sheep retrovirus (JSRV) infection was performed by polymerase chain reaction (PCR) of blood DNA samples collected from 40 sheep and goats in 10 different flocks in Hokkaido, the northern island of Japan. No exogenous (oncogenic) JSRV sequence was detected by PCR in these samples, while the ovine endogenous retrovirus sequence was successfully amplified in all samples. Our paper is the first demonstration of JSRV surveillance in Japan and shows no evidence of oncogenic JSRV infection in sheep and goats in Hokkaido. 相似文献
9.
Naoyoshi Kawano Evangelina Ella Osamu Ito Yasuo Yamauchi Kiyoshi Tanaka 《Soil Science and Plant Nutrition》2013,59(5):659-665
The mechanism of flash flood tolerance was investigated by using a flash flood-intolerant rice cultivar (Oryza sativa), IR42 and a tolerant one, FR13A. The photosynthetic rate of the rice seedlings from both types of cultivars decreased during submergence, whereas the photosynthetic rate of FR13A remained higher than that of IR42. The decrease in photosynthesis in IR42 was partially due to a higher inhibition of the activities of ribulose-1,5-bisphosphate carboxylase, fructose-1,6-bisphosphatase, and glyceraldehyde-3-phosphate dehydrogenase, than in FR13A. After submergence receded (hereafter referred as “desubmergence”), both types of submerged rice cultivars experienced oxidative damage. However the oxidation of the lipids and proteins in FR13A was maintained at a lower level than that in IR42 after desubmergence. On the other hand, the superoxide dismutase activity in FR13A increased more than that in IR42 after desubmergence. Moreover, the content of total ascorbate (reduced and oxidized ascorbates), a main antioxidant increased in FR13A, while in IR42 the content remained low after desubmergence. It is assumed that the photosynthetic activity during submergence may be related to flash flood tolerance. Moreover, flash flood tolerance requires a rapid stimulation of antioxidant systems after desubmergence. 相似文献
10.
Naoyoshi Nishibori Akihiko Yuasa Motosuke Sakai Shinsuke Fujihara Sachio Nishio 《Fisheries Science》2001,67(1):79-83
SUMMARY: Polyamines are widely distributed in nature and known to have many roles in living organisms. We investigated the concentrations of polyamines together with inorganic nutrients during a summer bloom period in the Seto Inland Sea of Japan. Putrescine and spermidine were the major polyamines in the coastal seawater. The concentrations at 1 m depth varied widely during the sampling period and ranged from 2.0 to 32.6 nM and 1.0 to 14.1 nM. Spermine concentrations were much lower than putrescine and spermidine. In addition, other polyamines (diaminopropane, cadaverine, norspermidine, homospermidine, norspermine) were also detected. Putrescine and spermidine seemed to be significant compounds in dissolved organic nitrogen in coastal seawater. 相似文献