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Jaime Maldonado Pere Riera Marta Sitja Narcis Saubi Enric Espuna Carlos Artigas 罗天海 《国外畜牧学(猪与禽)》2006,26(6):35-38
本文报道了对西班牙养猪密集地区进行的猪流感病毒(SIV)血清学和病毒学调查结果,其目的是检测H1N2亚型猪流感病毒是否和在欧洲其它地区一样也流行于这些地区。在西班牙北部和东部地区100个未接种猪流感疫苗的繁育群采集了600份母猪血清,以进行抗H1N1、H3N2和H1N2亚型病毒的血凝抑制试验(HI);再借助鸡胚病毒分离法和商品薄膜免疫分析法,对采自有呼吸道疾患的225份病猪肺样本进行检测以确定是否存在猪流感病毒。通过HI和逆转录酶-聚合酶链式反应(RT-PCR)方法,再辅以cDNA部分序列的测定,对分离毒株进行鉴定。对血清所作的HI试验表明,在83%受测猪群和76·3%受测猪中至少存在抗一种猪流感病毒亚型的抗体。在受检的600份母猪血清中,仅含抗H1N2、H3N2或H1N1亚型病毒抗体的样本数分别为109份(占总样本比例的18·2%)、60份(10%)和41(6·8%)。从有呼吸道疾患的猪肺样本中分离到12株H3N2亚型病毒、9株H1N1亚型病毒和1株H1N2病毒。对H1N2亚型分离株的神经氨酸酶基因436个核苷酸序列进行的分析,进一步证实了其身份。显然,猪流感依然流行于所研究猪群中,同时一种新的亚型病毒(即H1N2亚型病毒)可能正在开始流行,并且也在西班牙引起了临床发病。 相似文献
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Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland 总被引：35，自引：0，他引：35
Allan GM Mc Neilly F Meehan BM Kennedy S Mackie DP Ellis JA Clark EG Espuna E Saubi N Riera P Bøtner A Charreyre CE 《Veterinary microbiology》1999,66(2):115-123
A porcine circovirus (PCV) was isolated from tissues of pigs with wasting syndromes from Spain, Denmark and N. Ireland. The antigenic profiles of these viruses were determined by indirect immunofluorescence assays using polyclonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and used for the genotyping of these PCV isolates. These PCV isolates were found to be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1). 相似文献
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Maldonado J Van Reeth K Riera P Sitjà M Saubi N Espuña E Artigas C 《Veterinary journal (London, England : 1997)》2006,172(2):377-381
This paper reports on a serological and virological survey for swine influenza virus (SIV) in densely populated pig areas in Spain. The survey was undertaken to examine whether the H1N2 SIV subtype circulates in pigs in these areas, as in other European regions. Six hundred sow sera from 100 unvaccinated breeding herds across Northern and Eastern Spain were examined using haemagglutination inhibition (HI) tests against H1N1, H3N2 and H1N2 SIV subtypes. Additionally, 225 lung samples from pigs with respiratory problems were examined for the presence of SIV by virus isolation in embryonated chicken eggs and by a commercial membrane immunoassay. The virus isolates were further identified by HI and RT-PCR followed by partial cDNA sequencing. The HI test on sera revealed the presence of antibodies against at least one of the SIV subtypes in 83% of the herds and in 76.3% of the animals studied. Of the 600 sow sera tested, 109 (18.2%), 60 (10%) and 41 (6.8%) had SIV antibodies to subtype H1N2 alone, H3N2 alone and H1N1 alone, respectively. Twelve H3N2 viruses, 9 H1N1 viruses and 1 H1N2 virus were isolated from the lungs of pigs with respiratory problems. The analysis of a 436 nucleotide sequence of the neuraminidase gene from the H1N2 strain isolated further confirmed its identity. Demonstrably, swine influenza is still endemic in the studied swine population and a new subtype, the H1N2, may be becoming established and involved in clinical outbreaks of the disease in Spain. 相似文献
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