排序方式： 共有48条查询结果，搜索用时 316 毫秒
1.
Yoshida H Momoi Y Taga N Ide K Yamazoe K Iwasaki T Kudo T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):663-669
Dendritic cells (DCs) are the most potent antigen-presenting cells that are expected to be therapeutic agents for tumor immunotherapy. In this study, we generated DCs of sufficient number for DC-based immunotherapy from peripheral blood mononuclear cells (PBMC) in dogs. PBMC were cultured in the presence of phytohemagglutinin (PHA). On day 6, large adherent cells with dendrite-like projections were seen, and the number of these large cells with projections increased on day 8. These cells were positive for esterase staining. They expressed MHC class II, CD11b, CD8 and weakly CD4 on their surface. They tended to make contact with lymphocytes under culture conditions. We obtained about 2-5 x 10(6) of DCs from 10 ml of peripheral blood. These DCs phagocytosed HEK-293 cells by overnight co-culturing. These cells generated from PBMC are possible canine DCs and are applicable to clinical trials of DC-based whole tumor cell immunotherapy in dogs. 相似文献
2.
Tsukasa OKANO Katsuhi NAKATA Yumiko NAKAYA Takashi NAGAMINE Manabu ONUMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):637-642
The Ryukyu long-furred rat, Diplothrix legata, is a large rodent
distributed only on Amami-ohshima Island, Tokuno-shima Island and Okinawa-jima Island,
Japan. This animal is endangered as a result of deforestation, predation by introduced
carnivores and mortality caused by vehicles. We performed theriogenological examinations
of 32 male and 25 female Ryukyu long-furred rats carcasses collected from wild populations
on northern Okinawa-jima Island from December 2005 to September 2013. Adult males had
remarkably large preputial glands. Seminiferous diameter of adult was significantly small
(136 ± 28 µm, n=8) from April to August. Numerous spermatozoa were
observed from September through February, and seminiferous diameter was significantly
large (216 ± 27 µm, n=12) during this time in adults; testes length
changed in a similar pattern. These findings indicate that the mating season may occur
from September through February. Size (body length) at sexual maturity was estimated to be
>560 mm in both sexes. From observation of corpora lutea and placental scars, litter
size was estimated to range from 2 to 12 (average=6, n=4). These results provide
fundamental knowledge that will be beneficial for in situ and ex
situ conservation of this rare species. 相似文献
3.
4.
5.
6.
Capsid size distributions of viral communities in the floodwater of a Japanese paddy field under a long-term fertilizer trial were surveyed during the rice cultivation period by using transmission electron microscopy. The capsid size distributions were monophasic, and the median values fell within the range of 50-70 nm. The quartile intervals were narrow from either 40-50 nm or 50-60 nm to either 60-70 nm or 70-80 nm for most samples. There was no clear seasonal variation in the capsid size distribution of viral communities. The difference in size distributions among different fertilizer plots was also not found. Viral communities in the floodwater were predominated by isometric icosahedral forms. Viruses with octahedral capsids and elongated ones were rare and sporadic in the floodwater. 相似文献
7.
Arai KY Tanaka Y Taniyama H Tsunoda N Nambo Y Nagamine N Watanabe G Taya K 《Domestic animal endocrinology》2006,31(1):19-34
In this study, the expression patterns of inhibins, activins, insulin-like growth factor-I (IGF-I) and steroidogenic enzymes in equine placentae recovered during the latter two-thirds of gestation were examined. Concentrations of inhibin A and inhibin pro-alphaC in endometrial and fetal placental tissue homogenates were very low during the period examined, whereas these tissues contained high concentrations of activin A. In both maternal endometrial and fetal placental tissues, activin A levels decreased as pregnancy progressed. Expression of inhibin alpha-subunit was not observed in the placenta using either immunohistochemistry or in situ hybridization. Inhibin/activin betaA-subunit and its mRNA were confined to maternal endometrial glands, whereas immunopositive betaB-subunit was not detected in either endometrial glands or microcotyledons. Cytochrome P450 side chain cleavage enzyme was detected by immunohistochemistry in both endometrial glands and microcotyledons, whereas cytochrome P450 17alpha-hydroxylase/lyase was absent in these tissues. Immunopositive signals for 3beta-hydroxysteroid dehydrogenase and cytochrome P450 aromatase were localized in microcotyledons but not in endometrial glands. Immunohistochemistry revealed that IGF-I was highly expressed in microcotyledons around Day 130, and decreased as pregnancy progressed. Changes in the expression of IGF-I were correlated with the number of PCNA positive cells in the placenta. The present study demonstrated the presence and localized the site of expression of activin, IGF-I and steroidogenic enzymes in equine placental tissues during the latter two-thirds of gestation; the results suggest that activin and IGF-I may be involved in the regulation of placental development. 相似文献
8.
Masuda T Odaka Y Ogawa N Nakamoto K Kuninaga H 《Journal of agricultural and food chemistry》2008,56(2):597-601
Lemongrass is a popular Asian herb having a lemon-like flavor. Very recently, potent tyrosinase inhibitory activity has been found in lemongrass in addition to various biological activities reported in the literature. The aim of the present study is to identify the active compounds in the lemongrass. An assay-guided purification revealed that one of the active substances was geranic acid. Geranic acid has two stereoisomers, which are responsible for the trans and cis geometry on the conjugated double bond. Both isomers are present in the active ethyl acetate-soluble extract of the lemongrass, and their IC50 values were calculated to be 0.14 and 2.3 mM, respectively. The structure requirement of geranic acid for the potent tyrosinase inhibitory activity was investigated using geranic acid-related compounds. 相似文献
9.
Analysis of Phytosterol,Fatty Acid,and Carotenoid Composition of 19 Microalgae and 6 Bivalve Species
Risako Hikihara Yasuhiro Yamasaki Tomoyuki Shikata Natsuko Nakayama Setsuko Sakamoto Sueo Kato 《Journal Of Aquatic Food Product Technology》2020,29(5):461-479
ABSTRACTIn this study, the functional components of 19 microalgae and 6 bivalve species were investigated in the context of the application in bivalve feeding and human health food. Principal component analysis was performed to detect any association between the functional components and individual microalgal species or taxonomic group. The proportions of the functional components differed depending on the taxonomic group or species of microalga. The genera Cheatoceros, Thalassiosira, and Isochrysis contained high concentrations of fucosterol and fucoxanthin, which are present in large amounts in brown algae. Diatoms, haptophytes, and eustigmatophytes, which are used as feed for bivalves, were rich in fucosterol and eicosapentenoic acid (EPA); further, haptophytes were rich in docosahexaenoic acid (DHA). In addition, the microalgae associated with red tide, i.e., the raphidophytes, were found to be rich in fucoxanthin, β-sitosterol, and EPA, whereas dinoflagellates were rich in DHA. Seven bivalve species also contained high concentrations of fucosterol, EPA, and DHA, as did microalgae, which were used to feed by bivalves. These results are useful in selecting microalgae effectively as feed of the bivalves. 相似文献
10.
Adachi I Kusuda S Kawai H Ohazama M Taniguchi A Kondo N Yoshihara M Okuda R Ishikawa T Kanda I Doi O 《The Journal of reproduction and development》2011,57(2):262-266
The purposes of the present study were to establish a noninvasive monitoring assay of fecal progestagen measurement to detect pregnancy and to identify the components of fecal progestagens in early, middle and late pregnancy in cheetahs. Feces were collected from 7 female cheetahs and analyzed from 30 days before the last copulation to parturition in 9 pregnancies. Blood was collected from one cheetah. Fecal progestagen and serum progesterone concentrations were determined by enzyme immunoassay (EIA). The profiles of the fecal progestagen concentrations were similar to the serum progesterone profile. Fecal progestagen and serum progesterone concentrations remained at the baseline until copulation. In the mean fecal progestagen profile during pregnancy (92.8 ± 0.4 days; from the last copulation to parturition), the concentrations increased 3-4 days after the last copulation and remained high until parturition. To investigate changes in the components of progestagen metabolites in the tripartite periods of gestation, fecal progestagens were analyzed by HPLC-EIA. Marked immunoreactive peaks consistent with 5α-pregnan-3α/β-ol-20-one and 5α-pregnan-3,20-dione and small peaks consistent with 5β-pregnan-3α/β-ol-20-one were detected. There were no distinct difference in the components of progestagens among the first, second and third trimesters of pregnancy. The hormone assay, as an indicator of fecal 5α-reduced pregnanes, is useful for detecting pregnancy and monitoring pregnant luteal activity in cheetahs. 相似文献