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R Heinz S.W Lee A Saparno R.N Nazar J Robb 《Physiological and Molecular Plant Pathology》1998,52(6):385-396
It is generally believed thatVerticilliummoves slowly up a plant through spore trapping sites in the vascular tissue in a stepwise fashion. In a resistant plant, rapid defence responses around these sites slow pathogen growth and often restrict the fungus to the root and stem base. In a susceptible plant, the fungus escapes and eventually proliferates logarithmically in the upper stem and leaves. In the present study Craigella tomatoes were infected withVerticillium albo-atrum. Amounts of pathogen were monitored in the upper stems by a PCR-based quantitative assay and S1nuclease protection was used to study expression of a fungal induced phenylalanine ammonia lyase gene (tPAL5) during a 2–21 day time course. Even in resistant plants fungal colonization and PAL gene expression were found to be fully systemic from the earliest sporulation events (i.e. 2–4 days post-inoculation) and to occur in cycles, the PAL gene cycle following the colonization cycle. Peaks of pathogen growth occurred at 2–4 days and 12–15 days, succeeded by intermittent periods of fungal elimination. The results indicated that wilt is more systemic and also more dynamic in terms of fungal population fluctuations than previously thought. 相似文献
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The increasing concentration of cadmium (Cd) in agricultural soil has resulted in crop productivity loss. The activation of the antioxidative enzyme system and its synergy with sulphur assimilation may be required as one of the mechanisms for the alleviation of the effects of Cd. In the present study the activities of antioxidative enzymes and sulphur assimilation were studied in Cd‐treated wheat (Triticum aestivum) cultivars to assess their involvement in determining yield potential. The cultivar WH542 (low yielding type) accumulated Cd to a greater amount in both root and leaf, and also exhibited higher contents of H2O2 and thiobarbituric acid reactive substance and the activity of superoxide dismutase (EC 1.15.1.1) than cultivar PBW343 (high yielding type). The activities of other antioxidative enzymes, catalase (EC 1.11.1.6), ascorbate peroxidase (EC 1.11.1.11), glutathione reductase (EC 1.6.4.2) and glutathione peroxidase (EC 1.11.1.9), activity of ATP‐sulfurylase (EC 2.7.7.4), sulphur content, photosynthetic, growth and yield characteristics were higher in PBW343 than in WH542 in Cd treatment compared to the control. The results suggest that the efficient functioning of enzymes of the antioxidative system and sulphur assimilation helped in alleviating the effects of Cd in PBW343, protected photosynthetic ability and maintained high yielding potential of the cultivar. 相似文献
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Gelfand MJ Raver JL Nishii L Leslie LM Lun J Lim BC Duan L Almaliach A Ang S Arnadottir J Aycan Z Boehnke K Boski P Cabecinhas R Chan D Chhokar J D'Amato A Ferrer M Fischlmayr IC Fischer R Fülöp M Georgas J Kashima ES Kashima Y Kim K Lempereur A Marquez P Othman R Overlaet B Panagiotopoulou P Peltzer K Perez-Florizno LR Ponomarenko L Realo A Schei V Schmitt M Smith PB Soomro N Szabo E Taveesin N Toyama M Van de Vliert E Vohra N Ward C Yamaguchi S 《Science (New York, N.Y.)》2011,332(6033):1100-1104
With data from 33 nations, we illustrate the differences between cultures that are tight (have many strong norms and a low tolerance of deviant behavior) versus loose (have weak social norms and a high tolerance of deviant behavior). Tightness-looseness is part of a complex, loosely integrated multilevel system that comprises distal ecological and historical threats (e.g., high population density, resource scarcity, a history of territorial conflict, and disease and environmental threats), broad versus narrow socialization in societal institutions (e.g., autocracy, media regulations), the strength of everyday recurring situations, and micro-level psychological affordances (e.g., prevention self-guides, high regulatory strength, need for structure). This research advances knowledge that can foster cross-cultural understanding in a world of increasing global interdependence and has implications for modeling cultural change. 相似文献
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Abubaker B. Ali Yan Haofang Li Hong Wu Yan You Nazar A. Elshaikh Gameraldawla Hussein 《Communications in Soil Science and Plant Analysis》2019,50(1):49-64
Biochar has recently received increased attention because it improves poor soil fertility. However, its potentiality to enhance soil physical properties under water stress conditions not yet deeply investigated. Hence, extensive field investigations were carried out to study the effects of biochar addition (BA) with deficit irrigation (DI) on soil bulk density (BD), porosity percentage (P%), soil moisture content (SMC%), soil hydraulic conductivity (K), cucumber yield and water use efficiency (WUE) during two consecutive seasons (2016 and 2017). The biochar treatments were B0 (0 ton ha?1), B1 (10 ton ha?1and B2 (20 ton ha?1), while the DI treatments were 1.0 (W1), 0.60 (W2) and 0.40 (W3) of the reference evapotranspiration (ET0). The parameters were measured at soil depths of 0–10 (d1), 10–20 (d2) and 20–30 cm (d3) for measurement periods of before sowing (P1), mid-season (P2) and after harvest (P3). The results showed that the B2W1 combination gave the highest yield (57 and 45.2 t ha?1), WUE (10.94 and 11.27 kg m?3), SMC (39.2 and 40.1%) in both seasons, respectively. The B2W3 had the highest porosity (47.5 and 46.1%) values at the d1. Meanwhile, the lowest soil BD values of 1.1 and 1.05 g cm?3 were obtained by the B2W1 at d1 for 2.16 and 2017, respectively. Statistically, most of the parameters studied under B2W2 and B0W1 had non-significant differences between them. Hence, the addition of biochar with DI could be an integrated approach to address the drought stress, while enhancing soil and plant properties. 相似文献
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Clemens Scheer Reiner Wassmann Kirsten Kienzler Nazar Ibragimov Ruzimboy Eschanov 《Soil biology & biochemistry》2008,40(2):290-301
Nitrous oxide emissions were monitored at three sites over a 2-year period in irrigated cotton fields in Khorezm, Uzbekistan, a region located in the arid deserts of the Aral Sea Basin. The fields were managed using different fertilizer management strategies and irrigation water regimes. N2O emissions varied widely between years, within 1 year throughout the vegetation season, and between the sites. The amount of irrigation water applied, the amount and type of N fertilizer used, and topsoil temperature had the greatest effect on these emissions.Very high N2O emissions of up to 3000 μg N2O-N m?2 h?1 were measured in periods following N-fertilizer application in combination with irrigation events. These “emission pulses” accounted for 80–95% of the total N2O emissions between April and September and varied from 0.9 to 6.5 kg N2O-N ha?1.. Emission factors (EF), uncorrected for background emission, ranged from 0.4% to 2.6% of total N applied, corresponding to an average EF of 1.48% of applied N fertilizer lost as N2O-N. This is in line with the default global average value of 1.25% of applied N used in calculations of N2O emissions by the Intergovernmental Panel on Climate Change.During the emission pulses, which were triggered by high soil moisture and high availability of mineral N, a clear diurnal pattern of N2O emissions was observed, driven by daily changes in topsoil temperature. For these periods, air sampling from 8:00 to 10:00 and from 18:00 to 20:00 was found to best represent the mean daily N2O flux rates. The wet topsoil conditions caused by irrigation favored the production of N2O from NO3? fertilizers, but not from NH4+ fertilizers, thus indicating that denitrification was the main process causing N2O emissions. It is therefore argued that there is scope for reducing N2O emission from irrigated cotton production; i.e. through the exclusive use of NH4+ fertilizers. Advanced application and irrigation techniques such as subsurface fertilizer application, drip irrigation and fertigation may also minimize N2O emission from this regionally dominant agro-ecosystem. 相似文献
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Nazaré Pinto da Cunha Alessia Giordano Mario Caniatti Saverio Paltrinieri 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2009,38(2):230-241
Background: The Sysmex XT‐2000iV is a hematology analyzer that combines laser and impedance technology. Its usefulness for determining cell counts in canine and feline intracavitary effusions has not yet been studied. Objectives: The objectives of this study were to evaluate the analytical performance of the Sysmex XT‐2000iV for cell counts in effusions from dogs and cats, and to assess correlation with an impedance counter and concordance with diagnoses based on cytologic findings. Methods: Effusions (43 pleural, 23 peritoneal, 6 pericardial) were analyzed from 32 dogs and 34 cats. Total nucleated cell count (TNCC), HCT, and RBC count were determined on the Sysmex and compared with those obtained on an impedance counter (Hemat 8, SEAC). Imprecision, linearity, and limit of detection were determined for the Sysmex. An algorithm was designed using quantitative and qualitative data from the Sysmex to classify the effusions and the results were compared with diagnoses based on cytologic findings. Results: Intra‐assay and interassay coefficients of variation on the Sysmex were variable. Linearity of TNCC was ≥0.993 for dogs and cats, with the exception of effusions from cats with feline infectious peritonitis, which had delta (Δ) TNC values >3.0. In comparison with the Hemat 8, a proportional error was found for TNCC on the Sysmex. Effusion classification based on the algorithm was concordant with that obtained by cytologic examination in 43/72 (60%) samples. Discordant results usually were due to the misclassification of cells with similar morphology (such as mesothelial and carcinoma cells) in Sysmex scattergrams. Conclusion: The Sysmex XT‐2000iV provides a precise and accurate TNCC and has moderate concordance with cytologic findings for classifying canine and feline effusions. Although microscopic examination of effusions is necessary to achieve an accurate diagnosis, the Sysmex can provide preliminary information that may be helpful to cytopathologists. 相似文献
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Nazar A. Elshaikh 《Communications in Soil Science and Plant Analysis》2018,49(2):225-236
Using leaching to remove salt beyond the root zone by deep percolation requires more water to be added and this increases the stress on water resources. We hypothesized that biochar addition could mitigate salt stresses on plants and reduce the leaching fraction. For this purpose, seven irrigation water salinity levels with electrical conductivities of 1, 2, 4, 5, 6, and 7.0 dS m???-1 and tap water (0.75 dS m?1) as a control treatment coupled with three biochar applications at rates of 0% (B0), 5% (B1), and 10% (B2) were used in a randomized design with three replications. Irrigation practices were realized by considering the weight of each pot. The results revealed that the actual evapotranspiration (AET) values ranged from 30.56 to 13.83 L pot?1 for the S0B2 and B0S6 treatments. Also, the results revealed that the total water applied (TWA) for salt leaching decreased following the applications of biochar and increased with increase of? electrical conductivity. The analysis of variance showed significant differences among actual yield (AY), leaching yield (LY), and simulated yield (SY) under biochar addition and irrigation treatments. For B0, the greatest fruit yield (219.4 g pot?1) was obtained from the S0 under LY. Similar results were obtained for B1 and B2 under S0 in LY with maximum fruit yield of 250.7 and 277.7 g plant?1, respectively. Realized leaching fractions under non-biochar ranged from 5% to 78% whereas for B1 they ranged from 4% to 58%, while for B2 they ranged from 3% to 32%. Results of the water-use efficiency (WUE) showed that the lowest WUE0 and WUE1 (0.00 and 5.53 g L?1) were obtained under B0S6 whereas the highest values (7.93 and 11.1 g L?1) were observed under B2S0. Moreover, we found that biochar mitigated salinity stress in Okra by capturing transient sodium ions and reducing ion toxicity, which are preferentially absorbed onto the biochar surfaces, while releasing potassium, calcium, and magnesium ions from biochar into the soil solution. 相似文献
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Epinephelus tauvina (Forskål) has spawned naturally in captivity. The larvae were reared to metamorphosis using rotifers, Artemia nauplii, copepods and minced shrimp meat. The developmental stages from hatching through to metamorphosis are described. Larvae grew from 2.25 mm total length at hatching, to 31.40 mm by day 50. Two mortality peaks were observed: the first between days 4 and 5, and the second between days 24 and 35. The first peak corresponded to first feeding and the unsuitability of rotifers, and the second to the change from feeding Artemia nauplii to copepods, which were not available in sufficient quantities. 相似文献