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1.
Bone marrow samples were collected from the ribs of 20 healthy adult male Iranian camels (Camelus dromedarius). The bone marrow smears were stained using Wright's stain. Blood samples were collected at the same time for routine haematological examination. The development and morphology of the blood cells in the bone marrow of the camels were similar to those of other domestic species. The mean myeloid/erythroid ratio was 1.21, the mean erythroid percentage was 42.7% and the mean myeloid percentage was 52.0%. The mean percentage of other cells was 5.3%. The mean percentage of eosinophils in the myeloid series was higher than in other domestic species. Iron stores, estimated from Perl's stain, ranged from scant (1+) to moderate (3+) but most samples had 2+ iron content. All peripheral blood results were within reference ranges. 相似文献
2.
Hashemnia M Khodakaram-Tafti A Razavi SM Nazifi S 《Veterinary research communications》2012,36(1):47-55
The progressive morphohistopathologic changes, distribution pattern of lesions and ultrastructural characteristics in Eimeria arloingi infection were investigated in experimentally infected kids. The 18 newborn animals allocated to 3 equal groups. Two of groups,
A, B were inoculated with a single dose of 1 × 103 and1 × 105 sporulated oocysts of E. arloingi, respectively. At 7, 14, 21, 28, 35, and 42 days postinoculation (DPI), 1 kid from each group was necropsied for pathologic
and ultrastructural studies. Progressive lesions were present at 21, 28, 35 and 42 DPI in the jejunum, ileum, cecum with fewer
in the duodenum and proximal colon. The oocysts shedding begin between 16 to 18 DPI. Grossly, minimal changes were observed
at 21 DPI as few whitish plaques or nodules and advanced lesions at 42 DPI as pseudoadenomatous pattern in the mucosa and
a cerebriform pattern on the serosal surface of jejunum and ileum. Early histopathologic lesions due to schizogony phase were
including presence of intracytoplasmic developmental stages of the parasite such as trophozoites, immature to mature schizonts
and mild infiltration of inflammatory cells. In late lesions due to various stages of gametogony, the histological pattern
was mainly remarkable hyperplasia of the villi and crypts epithelial cells, eventually developed into papillary projections
of reactive epithelium. The mesenteric lymph nodes showed a few numbers of large schizonts in the cortical lacteals. This
study showed E. arloingi as a highly pathogenic species for kids, the incubation period was 16–18 days and the main target organ was jejunum with
characteristic morphohistopathologic lesions. 相似文献
3.
Glycosylated Porphyra-334 and Palythine-Threonine from the Terrestrial Cyanobacterium Nostoc commune
Ehsan Nazifi Naoki Wada Minami Yamaba Tomoya Asano Takumi Nishiuchi Seiichi Matsugo Toshio Sakamoto 《Marine drugs》2013,11(9):3124-3154
Mycosporine-like amino acids (MAAs) are water-soluble UV-absorbing pigments, and structurally different MAAs have been identified in eukaryotic algae and cyanobacteria. In this study novel glycosylated MAAs were found in the terrestrial cyanobacterium Nostoc commune (N. commune). An MAA with an absorption maximum at 334 nm was identified as a hexose-bound porphyra-334 derivative with a molecular mass of 508 Da. Another MAA with an absorption maximum at 322 nm was identified as a two hexose-bound palythine-threonine derivative with a molecular mass of 612 Da. These purified MAAs have radical scavenging activities in vitro, which suggests multifunctional roles as sunscreens and antioxidants. The 612-Da MAA accounted for approximately 60% of the total MAAs and contributed approximately 20% of the total radical scavenging activities in a water extract, indicating that it is the major water-soluble UV-protectant and radical scavenger component. The hexose-bound porphyra-334 derivative and the glycosylated palythine-threonine derivatives were found in a specific genotype of N. commune, suggesting that glycosylated MAA patterns could be a chemotaxonomic marker for the characterization of the morphologically indistinguishable N. commune. The glycosylation of porphyra-334 and palythine-threonine in N. commune suggests a unique adaptation for terrestrial environments that are drastically fluctuating in comparison to stable aquatic environments. 相似文献
4.
5.
S. Nazifi M. Mansourian B. Nikahval S. M. Razavi 《Tropical animal health and production》2009,41(1):129-134
Thyroid hormones might be able to regulate the activities of superoxide dismutase (SOD), catalase and glutathione peroxidase
(GPX). The role of thyroid hormones in metabolic pathways and antioxidant enzyme activities are well known in many species.
Nevertheless, there is no report describing probable relationship between thyroid hormones status, erythrocyte antioxidant
enzymes and serum profiles of trace elements. This study was undertaken to investigate the relationship between these parameters
in Iranian dromedary camels. Blood samples were taken from the jugular vein of 30 clinically healthy Iranian dromedary camels
under aseptic conditions during 6 consecutive days of summer. The serum was analyzed for serum profile of thyroid hormones,
trace elements, SOD and GPX activity. There were no significant differences in serum thyroid hormones, serum level of zinc
(Zn), copper (Cu), manganese (Mn), iron (Fe), selenium and antioxidant enzymes in different days (P > 0.05). There was a significant
negative correlation between SOD and Fe (P < 0.01, r = -493). There was no significant correlation between other parameters.
In case of copper deficiency likewise the present study, the copper was used to produce more SOD, so there was a decrease
in Fe transportation, and it might be a cause of decrease in Fe amount. On the other hand, a relatively small quantity of
Fe is present in tissue myoglobin, catalase, peroxidases, and cytochromes. So it might be another cause of decrease in Fe
amount. The explanation for these finding is not possible at this moment and further investigations are needed to interpret
these changes. 相似文献
6.
Nazifi S Ansari-Lari M Asadi-Fardaqi J Rezaei M 《Veterinary journal (London, England : 1997)》2009,182(2):315-319
Acute phase proteins (APPs) such as serum amyloid A (SAA), haptoglobin (Hp), and plasma fibrinogen (Fb) are diagnostic and prognostic biomarkers. This study determined the pattern of SAA in traumatic reticuloperitonitis (TRP) in cows, and compared the findings with Hp, and plasma Fb concentrations in the differential diagnosis of TRP using receiver operating characteristic (ROC) analysis. Blood samples were collected from 89 cows: 53 cases of TRP, 16 animals with other internal disorders and 20 healthy controls.SAA and Hp had a similar pattern and magnitude of changes in TRP. Using the ROC method, the optimal cut-off for the diagnosis of TRP was 68 μg/mL for SAA and 0.74 g/L for Hp, with 100% sensitivity and 86.1% specificity; either of these parameters may therefore be used as a sensitive and relatively specific tool for the differential diagnosis of TRP in cattle. The diagnostic accuracy of plasma Fb was significantly lower than either SAA or Hp (P < 0.01). The combined use of these APPs (whilst aware of their conditional interdependence) may not provide any additional diagnostic information than can be obtained using either SAA or Hp alone. 相似文献
7.
Nazifi S Saeb M Ghavami SM 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2002,49(1):9-12
Blood samples were obtained from 12 Iranian fat-tailed sheep during 7 weeks pre-partum, at parturition and 7 weeks post-partum. The lipids measured were cholesterol, triglyceride, total lipid, high-density lipoprotein (HDL)-cholesterol, low-density lipoprotein (LDL)-cholesterol, and very low-density lipoprotein (VLDL)-cholesterol. The concentrations of cholesterol, triglyceride, HDL-cholesterol and VLDL-cholesterol during the 7 weeks pre-partum, at parturition and the 7 weeks post-partum were significantly different (P < 0.05). One week before parturition, the concentrations of cholesterol, triglyceride, HDL-cholesterol and VLDL-cholesterol were higher (P < 0.05) than at other periods. The lowest concentrations of these parameters were observed 2-3 weeks after parturition. In this study, significant positive correlations were observed between the time of sampling (pre-partum, parturition and post-partum) and serum cholesterol (r = 0.22; P < 0.01) and HDL-cholesterol (r = 0.25; P < 0.01). 相似文献
8.
Nazifi S Razavi SM Mansourian M Nikahval B Moghaddam M 《Tropical animal health and production》2008,40(1):47-53
This study was carried out in two observational clinical studies. Study 1 comprised 50 adult crossbred cattle naturally infected
by Theileria annulata. Infected animals were divided into 4 subgroups with different parasitaemia (<1%, 1–3%, 3–5% and >5%). Study 2 comprised
20 adult crossbred cattle naturally infected by Anaplasma marginale. Infected animals were divided into 3 subgroups with different parasitaemia (<10%, 10–20% and 20–30%). In study 1, a significant
negative correlation (P < 0.001) was observed between parasitaemia and superoxide dismutase (SOD). Positive correlations (P < 0.001)
were observed between parasitaemia and lactate dehydrogenase (LDH) and mean corpuscular fragility (MCF). In study 2 positive
correlations (P < 0.05) were observed among parasitaemia and MCF and LDH activity. SOD activity had a negative correlation
with parasitaemia in cattle with parasitaemia lower than 10% but no significant correlation (P > 0.05) was observed between
SOD activity and parasitaemia in cattle with 10–20 and 20–30% parasitaemia. In comparison of both studies we came to the conclusion
that in theileriosis as the severity of disease increased the anaemia, MCF and LDH activity increased and SOD activity decreased
at any parasitaemia, but in anaplasmosis the anaemia, MCF and LDH activity increased at any parasitaemia but SOD activity
decreased only in early but not in advanced stages of disease. 相似文献
9.
Subclinical ketosis (SCK) is simply a condition marked by increased levels of circulating ketone bodies without the presence of the clinical signs of ketosis. Subclinical ketosis can cause economic losses through decreased milk production and association with preparturient diseases. Limited information is available regarding the prevalence of SCK in dairy herds in Southwestern Iran. The objectives of this study were (i) determination of the cutoff point of nonesterified fatty acids (NEFAs) and glucose concentrations for diagnosis of SCK using receiver operating characteristic (ROC) analysis, and (ii) determination of prevalence of subclinical ketosis in apparently healthy dairy cattle in Southwestern Iran. From October to December 2009, a total of 100 clinically healthy multiparous Holstein cows (3-8 years old) were randomly selected from 16 dairy herds around Kazerun, Fars Province, Iran. The cows had two-six lactations, with body weight ranging from 500 to 650 kg. Blood samples for each cow were taken at 2, 4 and 6 weeks post parturition and 3-4h after the morning feeding. The optimal cutoff point was set, by the ROC method, to >0.26 mmol/L for NEFA, and < 2.26 mmol/L for glucose with corresponding 82.54% sensitivity and 91.89% specificity for NEFA and 44.44% sensitivity and 78.38% specificity for glucose. Cows with BHB concentrations higher than 1200 μmol/L were classified as having SCK. In 2, 4 and 6 weeks post parturition 63%, 68% and 59% of the tested cows were subclinically ketotic. Overall, 97% of tested cows (97/100) were considered subclinically ketotic in at least one sample period. Thirty percent of tested cows (30/100) suffered from subclinical ketosis in all of the 2, 4 and 6 weeks postpartum. The results suggest that, a cut-off point of 0.26 mmol/L for NEFA concentrations can be used during early lactation for diagnosis of subclinical ketosis and making management decisions for prevention and treatment. Glucose cannot be a good criterion for diagnosis of SCK and it does not appear to be useful for monitoring subclinical ketosis. 相似文献
10.
Mohebbi-Fani M Mirzaei A Nazifi S Shabbooie Z 《Tropical animal health and production》2012,44(2):259-265
This study assessed the changes of plasma vitamin A, E, and C and the lipid peroxidation status of sheep during breeding and
pregnancy under drought conditions. The study was conducted on 105 cross-bred fat tailed ewes, 3–5 years old with body condition
scores (BCS) of 2.5 to 3.5. The ewes were grazing on medium-to-low quality forages during summer and low quality forages within
the succeeding months and had ad libitum access to a mixture of alfalfa hay (40%) and wheat straw (60%) in the afternoons. From 3 weeks before breeding till 1 month
after the introduction of rams, 300 g of barley grain/head/day was offered to the ewes and then the supplemental grain was
reduced to 100 g/head/day. For better synchronization of estrus cycles in ewes, they were isolated from the rams for at least
2 months and then kept in close proximity of the rams for 1 week before the introduction of the rams to the ewe flock. Then,
whole blood samples were collected on days 1, 7, 21, and 120 after ram introduction. Vitamins A, E, and C were measured in
plasma. Malondialdehyde (MDA) was measured in the hemolysate as a biomarker of lipid peroxidation. Plasma progesterone (P4)
was measured in the samples of day 120 for assessing pregnancy status of the ewes. Vitamins A and C showed continuous and
significant declines (P < 0.05) through days 1 to 120. Vitamin E declined only during the first 21 days of the study and remained almost constant
till day 120. MDA concentration increased significantly at day 21. An elevated concentration of MDA was also detected at day
120. The difference between days 21 and 120 was not significant (P > 0.05). A positive correlation between vitamins E and C was detected at day 120 (r = 0.349, P < 0.01). Age and BCS did not affect the patterns of changes. Assuming that the ewes with P4 concentrations ≥2.5 ng/ml were
pregnant, 95 out of 105 ewes (90.5%) were pregnant at day 120 of the study. Under the conditions of the present study with
medium-to-low quality pastures as the main sources of feed, ewes of various ages and body conditions may suffer from oxidative
stress during breeding and pregnancy. 相似文献