Surgical technique for adrenalectomy in bovines (Bos bubalis)     

M Manohar  A G Singh  J M Nigam 《The British veterinary journal》1972,128(10):lv-lvii

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Inheritance of albinism in certain interspecific and intersubspecific crosses in groundnut (Arachis hypogaea L.)     

S. L. Dwivedi  S. N. Nigam  S. K. Pandey  R. W. Gibbons 《Euphytica》1984,33(3):705-708

Summary A study of the several interspecific and inter-subspecific crosses indicated that albinism in groundnut is recessive and in these crosses is controlled by triplicate recessive loci.ICRISAT Paper No. JA-336  相似文献   

Inheritance of leaf angle in Triticum aestivum L.     

S. N. Nigam  J. P. Srivastava 《Euphytica》1976,25(1):457-461

Summary A 6×6 diallel was prepared to study the inheritance of leaf angle in T. aestivum L. Genetic analysis in terms of diallel cross parameters and graphic analysis indicated the control of additive gene effects in the expression of this character. The results of F₁ analysis were supported by the analysis of F₂ data.  相似文献   

Inheritance of traits associated with seed size in groundnut (<Emphasis Type="Italic">Arachis hypogaea</Emphasis> L.)     

R. Venuprasad  R. Aruna  S. N. Nigam 《Euphytica》2011,177(2):169-177

A new wheat-Thinopyrum substitution line AS1677, developed from a cross between wheat line ML-13 and wheat-Thinopyrum intermedium ssp. trichophorum partial amphiploid TE-3, was characterized by fluorescence in situ hybridization (FISH), sequential Giemsa-C banding, genomic in situ hybridization (GISH), seed storage protein electrophoresis, molecular marker analysis and disease resistance screening. Sequential Giemsa-C banding and GISH using Pseudoroegneria spicata genomic DNA as probe indicated that a pair of St-chromosomes with strong terminal bands were introduced into AS1677. FISH using pTa71 as a probe gave strong hybridization signals at the nuclear organization region and in the distal region of the short arms of the St chromosome. Moreover, FISH using the repetitive sequence pAs1 revealed that a pair of wheat 1D chromosomes was absent in accession AS1677. Seed storage proteins separated by acid polyacrylamide gel electrophoresis (APAGE) and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that AS1677 lacked the gliadin and glutenin bands encoded by Gli-D1 and Glu-D1, further confirming the absence of chromosome 1D. The introduced St chromosome pair belonging to homoeologous group 1 was identified by newly produced genome specific markers. AS1677 is a new 1St (1D) substitution line. When inoculated with stripe rust and powdery mildew isolates, AS1677 expressed stripe rust resistance possibly derived from its Thinopyrum parent. AS1677 can be used as a donor source for introducing novel disease resistance genes to wheat in breeding programs aided by molecular and cytogenetic markers.  相似文献   

Malolactic fermentation in wine with Lactobacillus casei cells immobilized on Delignified cellulosic material     

Agouridis N  Bekatorou A  Nigam P  Kanellaki M 《Journal of agricultural and food chemistry》2005,53(7):2546-2551

In this work Lactobacillus casei ATCC 393 cells immobilized on delignified cellulosic material (DCM) were used for malolactic fermentation (MLF) of wine. Wine was produced using yeast cells immobilized on DCM at 20 degrees C, and after alcoholic fermentation, MLF at 27 degrees C followed using immobilized L. casei ATCC 393 cells. A total of 11 repeated alcoholic and subsequent MLF batches were performed within a period of 1 month. As the repeated MLF batches proceeded, the MLF activity of the immobilized biocatalyst was reduced. Malic acid degradation was reduced from 80 to 2%, pH was reduced by 0.5-0.1 unit, acetic acid concentrations were slightly reduced or remained stable (0.002 g/L), the higher alcohols 1-propanol, isobutyl alcohol, and amyl alcohol were decreased by 84, 23, and 11%, respectively, and ethyl acetate concentration was increased by approximately 56%. Wine samples were analyzed by GC-MS before and after MLF, revealing some qualitative differences.  相似文献   

Food additive lactic acid production by immobilized cells of Lactobacillus brevis on delignified cellulosic material     

Elezi O  Kourkoutas Y  Koutinas AA  Kanellaki M  Bezirtzoglou E  Barnett YA  Nigam P 《Journal of agricultural and food chemistry》2003,51(18):5285-5289

Improvements in yield and productivity in lactic acid fermentation by Lactobaccilus brevis cells immobilized on delignified cellulosic (DC) material are reported. The system proved to be more efficient in comparison with the work reported by other workers. Yields of 80 and 100% conversion using glucose were obtained at 30 degrees C in 1 day of fermentation time. Lactic acid fermentation using whey as substrate was obtained at 30 degrees C in 1-1.5 days, resulting in 70% yield, whereas the remaining lactose in whey was converted to alcohol byproduct, leading to a 90% lactose exploitation and 100% conversion. Cell immobilization of L. brevis on DC material was proved by its reuses in repeated batch fermentations and through electron microscopy. A series of 10 repeated batch fermentations without any loss in cell activity showed a tendency for high operational stability. The presence of DC material resulted in a drastic drop of the fermentation time from 48 to 13 h.  相似文献   

Study on the fracture repair of metatarsus in bovine with special reference to homogenous bone plating.     

A P Singh  J M Nigam 《The Indian veterinary journal》1979,56(3):214-218

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ARTERIOGRAPHIC ANATOMY OF THE ABDOMINAL AORTA IN THE GOAT, DOG, PIG, AND RABBIT     

Ambika P.  Singh  MVSc  PhD  Gaj Raj  Singh  MVSc  Dwarika N.  Sharma  MVSc  PhD  Janki M.  Nigam  MVSc  PhD  Amar K.  Bhargava  MS  PhD 《Veterinary radiology & ultrasound》1982,23(6):279-281

Arteriographic anatomy of the major branches of the abdominal aorta of rabbits, dogs, pigs, and goats was investigated. The origin and location of the celiac, cranial mesenteric, right renal, left renal, caudal mesenteric, and external iliac arteries were demonstrated, and their placement was described in relation to the iliac bifurcation and lumbar vertebral bodies.  相似文献   

ANGIOGRAPHIC EVALUATION OF BOVINE FOOT ABNORMALITIES     

S. N. Gogoi  J. M. Nigam  A. P. Singh 《Veterinary radiology & ultrasound》1982,23(4):171-174

Angiography of normal and diseased feet in cattle was carried out. In osteomyelitis of distal phalanx there was a narrowing of the digital artery with diminished vascular supply to the affected digit. An area of bone necrosis was found to be avascular. With overgrown hooves, narrowing of both the medial and lateral digital arteries with ill-defined laminar vessels was seen. The vascular pattern in cattle with cork screw claws was characterized by a dilated tortuous digital artery and a crowded arterial network resulting in increased vascular supply in the affected claw. Abrupt stricture of common digital artery, a crowded vascular network in the area of the middle phalanx and filling defects of terminal arches were demonstrated in animals with exostosis  相似文献   

花生AFLP指纹图谱   总被引：40，自引：4，他引：36  

翁跃进  Santosh Gurtu  S N Nigam 《中国油料作物学报》1999,21(1):10-12

ＡＦＬＰ是一种以ＰＣＲ为基础的ＤＮＡ指纹图谱技术。利用ＡＦＬＰ技术对从国际半干旱所（Ｉ－ＣＲＩＳＡＴ）引进的９份花生抗旱品种绘制指纹图谱,通过引物Ｅ—ＡＣＡ和与之匹配的ＭＣＡＧ和Ｍ—ＣＡＴ,在３００～６０００ｂｐ的范围内共获得１５７７条ＡＦＬＰ扩增产物,每个品种有主带和次带至少７１条之多,其中１０条为多态性的带纹。  相似文献