排序方式： 共有36条查询结果，搜索用时 25 毫秒
1.
Morteza Bagheri Isa Abdi Rad Nima Hosseini Jazani Rasoul Zarrin Ahad Ghazavi 《Iranian Biomedical Journal》2015,19(3):183-187
Background:
The variable numbers of tandem-repeat (VNTR) alleles at the phenylalanine hydroxylase (PAH) gene have been used in carrier detection and prenatal diagnosis in phenylketonuria families. This study was carried out to analyze VNTR alleles at the PAH gene in Iranian Azeri Turkish population.Methods:
In this study, 200 alleles from general population were studied by PCR.Results:
The frequencies of VNTR alleles were 45%, 46%, 2%, 3%, 1%, and 3% in studied group regarding 3, 8, 9, 11, 12, and 13 repeat copies, respectively. Statistically significant differences were not found between expected and observed frequencies of VNTR genotypes (P > 0.05).Conclusions:
VNTR alleles with three and eight repeats were frequent, and the VNTR alleles with 13 repeats showed 3% frequency in the tested group. This study is the first report on tested population genetic structure using VNTR alleles at the PAH gene. Key Words: Phenylalanine hydroxylase, Population genetics, Variable numbers of tandem-repeat 相似文献2.
Mohammadzadeh Sedigheh Milla Sylvain Ahmadifar Ehsan Karimi Masoumeh Dawood Mahmoud A. O. 《Fish physiology and biochemistry》2021,47(4):951-960
The use of recombinant gonadotropin-releasing hormone (rGnRH) has very rarely been tested in fish to promote spawning. This study evaluated the impact of recombinant chicken gonadotropin-releasing hormone (rcGnRH) with metoclopramide on the release of sex steroids and final maturation induction in goldfish (Carassius auratus) broodstock. For this purpose, goldfish broodstock was divided into four groups and treated with 0.9% NaCl with 20 mg/kg metoclopramide (Met) (C); 10 μg/kg body weight (BW) rcGnRH with 20 mg/kg metoclopramide (rcGn10); 15 μg/kg BW rcGnRH with 20 mg/kg metoclopramide (rcGn15); and 20 μg/kg BW rcGnRH with 20 mg/kg metoclopramide (rcGn20). The capability of the rcGnRH for eliciting biological response was tested in vivo by evaluating the changes of 17β estradiol (E2), testosterone (T), and 17α, 20β-dihydroxy-4-pregnen-3-one (DHP) and the induced spawning. Blood samples were obtained at 0 h, 12 h, and 24 h after injection. The rcGn10, rcGn15, and rcGn20 treatments induced lower E2 concentration, especially 24 h post-injection. T levels were significantly higher in rcGn10, rcGn15, and rcGn20 treatments 12 h post-injection than at 0 h and then decreased at 24 h post-injection. Furthermore, the rcGnRH tested significantly enhanced DHP secretion in rcGn10, rcGn15, and rcGn20 treatments 12 h post-injection before a decline at 24 h post-injection. No significant difference between the sampling times was found in the C treatment for the 3 sex steroids tested. The results also displayed that rcGnRH at 10–20 µg/kg of body weight can trigger spawning with the highest speed and efficiency of spawning at 20 µg/kg. The obtained results represent a possible strategy for enhancing the artificial reproduction and ovulation of broodstock fish by rGnRH and further support the use of recombinant hormones to promote reproduction in aquaculture.
相似文献3.
利用木耳菌糠栽培元蘑技术的研究 总被引：1，自引：1，他引：0
为了解决黑龙江省每年大量木耳菌糠得不到有效利用的实际问题,并积极探索元蘑代料栽培的新技术,以传统栽培种配方CK为对照,以12%的浓度梯度逐渐用菌糠代替木屑设计6个栽培种配方,等量接种生长状况一致的原种,在相同的条件下发菌与出菇。通过采用测定元蘑菌丝的生长势、满袋天数、原基形成时间、成菇状况、子实体的长势及生物学转化效率的方法,研究了栽培料中不同菌糠浓度对元蘑生长周期、生物学效率以及品质的影响。结果表明：栽培种配方为木耳菌糠36%、木屑44%、麦麸17%、黄豆粉1%、玉米面1%、碳酸钙1%,适宜元蘑生长。其菌丝生长浓密健壮,生物学转化效率为81.96%,比对照高出1.74%,试验证明了利用木耳菌糠栽培元蘑是可行的。 相似文献
4.
Fatemeh Majidi Hadi Bamehr Zohreh Shalchian Mohammad-Reza Kouchakian Nima Mohammadzadeh Ahmad Khalili 《Anatomia, histologia, embryologia》2020,49(3):359-364
Supplements produced by mouse testicular cells (mTCs) and the interaction between cells can increase the differentiation rate of human umbilical cord mesenchymal stem cells (hUCMSCs) into the germ-like cells. We studied the differentiation rate of hUCMSCs into the germ-like cells under effect of mTCs co-culturing. Isolated hUCMSCs from postpartum human umbilical cords were cultured. Then, the expression of mesenchymal (CD73, CD90 and CD105) and haematopoietic (CD34 and CD45) markers of hUCMSCs were confirmed by flow cytometry. Then, the hUCMSCs were cultured in four distinct groups: (a) control, (b) co-culture until D0, (c) co-culture until D5 and (d) co-culture until D10, in order to differentiate into the germ-like cells. After 10 days, the expression of OCT4, VASA, Fragilis and SYCP3 genes were examined by Real-Time qPCR. The flow cytometry indicated a high expression of mesenchymal markers and a low expression of haematopoietic markers (CD73:98.6%, CD90: 99.1%, CD105: 99.5%, CD34: 4.22% and CD45: 2.54%). The expression of OCT4 decreased during the time while the expression of VASA, Fragilis and SYCP3 markers increased in the co-culture with testicular cells (p value <.05). Co-culture with mTCs may be used as an effective method to differentiate hUCMSCs into germ-like cells. 相似文献
5.
Arash Mohammadzadeh Mojtaba Tavakoli Mohammad Reza Chaichi Babak Motesharezadeh 《Archives of Agronomy and Soil Science》2013,59(12):1765-1778
The effect of bacteria inoculation was studied on sunflower growth and phytoremediation capacity in soils contaminated by different levels of nickel. The experimental treatments consisted of four levels of bacteria inoculation – non-inoculated, inoculated by Bacillus safensis, inoculated by Kocuria rosea, and co-inoculated by B. safensis + K. rosea – and four levels of nickel concentrations in the soil – Ni0, Ni150, Ni300, and Ni450 (0, 150, 300, and 450 mg Ni per kg soil, respectively). The treatments were arranged as factorial structure based on a completely randomized design. Results have shown that growth indices, photosynthetic pigments, shoot Fe concentration, root and shoot Zn concentration, and translocation factor decreased as the Ni concentration in soil increased. Shoot and root Ni concentration were higher at Ni450, whereas, the highest Ni uptake by the plant was observed at Ni300 when the sunflower seed was co-inoculated by B. safensis + K. rosea bacteria. Bacteria inoculation significantly increased the plant growth, photosynthetic pigments, and Ni uptake. By B. safensis inoculation, the Fe concentration significantly increased in shoot, while it decreased in root. 相似文献
6.
1980-2009年西藏西北部潜在蒸散时空分布特征及其影响因素 总被引：3，自引：0，他引：3
通过分析西藏西北部潜在蒸散量的时空分布以及影响潜在蒸散的气候因素.结果表明:①西藏西北部潜在蒸散呈现从西北向东南减少的分布特征,最大潜在蒸散出现在夏季,春季次之,冬季最小.近30 a来,除冬季潜在蒸散呈略微增加趋势外,其余均呈减少趋势.气温、相对湿度和降水量呈增加趋势,平均风速和日照时数呈减少趋势;潜在蒸散与气温、风速、日照时数呈正相关,与相对湿度、降水量呈负相关.②影响潜在蒸散的主要气象要素为相对湿度、平均气温和日照时数,风速对冬季潜在蒸散的影响最为显著;近30 a来,风速减小、日照时数减少、相对湿度增加是导致潜在蒸散量减少的主要气象影响因子.西藏西北部地区潜在蒸散的变化存在“蒸发悖论”现象,即在气温升高[0.55℃·(10a)-1]的背景下,潜在蒸散呈下降趋势[10.02 mm ·(10a)-1]. 相似文献
7.
Comparison of the effects of Ham'sF10 and αMEM in combination with FBS or BSA in vitrification/warming solution on quality and viability of sheep ovarian follicles 
下载免费PDF全文
下载免费PDF全文 F Mohammadzadeh L Safdarian F Amidi A Mohammadzadeh K Mortezaee S Mehdinejhadiani A Sobhani S Ghasemi F Sargolzaei Aval 《Reproduction in domestic animals》2017,52(4):551-560
The aim of this study was to evaluate the effects of the two types of media, namely minimum essential medium (αMEM) and Ham'sF10, supplemented with foetal bovine serum (FBS) or bovine serum albumin (BSA) in vitrification/warming solution on the quality and viability of sheep ovarian follicles. Vitrification method was applied for cryopreservation of sheep ovarian cortex using Ham'sF10 and αMEM supplemented with either BSA or FBS. There were five groups: Fresh, Ham'sF10+ BSA, Ham'sF10+ FBS, αMEM + BSA and αMEM + FBS. Samples were cultured for two weeks after warming. Viability and morphology of follicles and DNA fragmentation in follicles and in tissue stroma cells were analysed before vitrification/warming and following one and two weeks of culture. The Ham'sF10+ FBS and Ham'sF10+ BSA groups showed a significant decrease in follicular viability after one week of culture (p < .05 vs. Fresh). Following two weeks of culture, all groups revealed a considerable fall in the number of viable follicles (p < .05 vs. Fresh). There was an increase in DNA fragmentation of connective tissue cells but not in the follicles (p < .05). Our results showed the better application of αMEM supplemented with BSA as a vitrification solution in improvement of cryopreservation effects and maintenance of follicular survival. 相似文献
8.
9.
Arash Mohammadzadeh Mojtaba Tavakoli Babak Motesharezadeh Mohammad Reza Chaichi 《Archives of Agronomy and Soil Science》2017,63(6):807-816
This paper describes the effect of plant growth-promoting bacteria on sunflower growth and its phytoremediation efficiency under Cd-contaminated soils. Four levels of bacteria inoculation (non-inoculation, inoculation by Bacillus safensis, Kocuria rosea and co-inoculation by Bacillus safensis+Kocuria rosea) and four Cd concentrations (0, 50, 100 and 150 mg Cd per kg soil) were arranged as factorial experiment based on a completely randomized design (CRD). Results showed that Cd significantly decreased growth by decreasing the shoot and root length and biomass (p < 0.01). In addition, Cd dramatically decreases photosynthetic pigments, Fe transport to shoot and Zn uptake (p < 0.01). Bacterial inoculation increased Fe and Zn uptake by plants, Cd concentration in the aboveground part of plants and Cd uptake by the enhancement of Cd concentration in plant tissue and biomass production. Results showed that the highest shoot Cd uptake was ?observed in ?inoculated plants by Bacillus safensis at Cd100 (20.35 mg pot?1). However, in average of Cd treatments, the performance of co-inoculation in Cd uptake (13.04 mg pot?1) was better than singular inoculation (10.68 and 12.58 mg pot?1 for Bacillus safensis and Kocuria rosea, respectively). Results revealed that bacterial inoculation increased the Cd uptake performance in shoot and total biomass by 30% and 25%, respectively. 相似文献
10.